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!!!"""####$$$%%%&&&&'''(((())))***++++,,,,----....////0000011112222333334444555556666677777788888999999999::::::::::;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 1d3232ef2600475c8d65b39bccfe0bfbSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-28_09-06_06723130Seq16716134697_134697mfagan96-Well K1313_G6_Chamaecrista_sp_matK-xFKB_3730_POP7_BDTv3.mobSeqPop7zbcflxyxzutuxwrgYK?62/,'       "/670$   )1551+((/:IWdoy 9m,gA`twgJ) 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"""!$!$!!="$"$WGxJ:LHxxxMxMxMxMMJxx`xxxxJMMxJMxJxxxJxMxxMxJMxMJxx~xMJMMMMMMxxMxJMMMMJMMMxJ~JMMJxMJxJxxMp8Lpp0op,aokddT9l^p)p,g\p,ppppppp,dpprp<,D,ppp,78pp ppdp1dd}ppppp{ppdpgVpLp8"lp6pkppp8X+ppd`,pJppdp8ppO,86,dp,8t  %'Pl6X!tVZ&Z*Ux@V"!yrSl bvi[N\MAG^x_dimpQrcylwnN+>cI?W0E TBZXFE/B-FD`4[8;^B8 q}]`cbwhMna\Wg7a~t/_}U1ihmpa"9]_Qp#^@8! L?%jDcGQ0vRu~Ygf>+BO |>A'rSb2xyIBlt7ygoVb9^9;|^913-1xIT;3l@.&:<`k+O3j<nA9/u{qmaHexBAr;5 Ua^2 AAT oif|Ct-Qh2>H[fCdU,_QE^U@C?}CC=)a15@p<W\NW`!i3Sy=q:)f\&03Y, iRER| A"R 0\2`w~ 64f^@o%}2DB%l;N[T"IkxhB)C1- CY|leO}&J*[szy=FEj<2>{eh^`IA?7|g&RRm ()a_z$Q LATTATTCACCGGTGAAGGTAGGCGCATACATCTGATATGTGAAATATGCACTTAGATTCCAGTCTAAACAACTAGGTAACATTGAACTCAGGCGATTAGACCCTGACGATACCGGCACCTGCAAGCACATAGGGATGATCGGACCCTACTTGTACCAGTAACAGTATTGTCGTAACACTAGGGTTTACATAGCTAGTCCCCCACTGGGTGTTATACGATAATAGGTCTGGTTCTATATTAGACCTATGTACGCGTATAAGTATCTCTCCCTCACCCTCTGTGTCACTATCATCCTAAGCAATACTCATATCATCTAGTGATCGGGGGATAAGTAGGAGAAATACGCTTTATAGTATACCCTTGAGGTGTCAAATATGAAAAAACTCTGTACAGTCTATTGAACGGCGAGACTCGCACGCTCAATATTTTATATATATAGTTAATATATACTCTCGCCACGGATACACGGTGACCATAAGTCGGGGTCAGTACTCTATCTATACCCTATCGCTATCTTACGCATATGCGTTGTCTTCGCCAGCATATACGTATCCACGGTATGGCGTAACAAACAGAAGGTTCTTAAAAACGTTGCGCCGATCCTCCTCTGTTGCCAGCTCTACGTACCGTATTACTCGTAAGTTATGTAAGGGGAGAAGCATATAGGGGAATATTCTCTTCTAAACATCTCGAGAGTAAAGTGTATTATAAACATAATATGTGCGCTCTGTCGAGAGCAGCTACATGATATTAGAGACATAGGGCGCAGTATGCACGTCGCTCTAATACATATATTCCTCTACCCCGAGAGGATCTAGTGGATTTATCTCATTATAATGTATAAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-28 11:10:02 -04:00AEPt@AEPt@APFN \'APXV2APrN 6APrV1APrX}}6ARTNASPFASPtASPtB1PtB1PtBCTSBufT PPCMNT 6CTIDP|CTNMPCTOwPCTTL 6CpEPDATA@DATA@DATA@DATA@DATA PDATA PDATA P-DATA P*}DATA /_\0DATA /_>DATA /_LDATA /_yZDCHTDSamDySN 6dDye#DyeN6oDyeN6tDyeN6yDyeN6~DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 5EVNT 6EVNT6EVNT6$FWO_GATCGTyp!!6CHCFGCEHCFGHCFGHCFG==InScInVtLANE$LAST~~ZLIMS!!6LNTD2LsrPaMCHNMODF6MODL3730NAVGNLNE`NOISpP1AMMP1RLM P1WDM?P2AMM sP2BAMM P2RLMPBASMMPBASMMأPCONMMPCONMMqPDMF\PDMF؇PLOCMPLOCM=PSZE`PTYPPPXLBRGNmPtRMXV1RMdNHPRMdV1RMdXH`RPrNPBRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT  -)RUNT  Rate 67RunN""PRS/N%hSCAN@SMED SMLtSMPL!![SPACMSPAC؀SPACMSVER4.0SVER SVER,,5Scal@Scan@TUBEG6Tmpr<UserphARphCH؞phDYbigphQLcphTRphTR0ZThis is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN \'APXV2APrN 6APrV1APrX}}6ARTNASPFASPtASPtB1PtB1PtBCTSBufT PPCMNT 6CTIDP|CTNMPCTOwPCTTL 6CpEPDATA@DATA@DATA@DATA@DATA PDATA PDATA P-DATA P*}DATA /_\0DATA /_>DATA /_LDATA /_yZDCHTDSamDySN 6dDye#DyeN6oDyeN6tDyeN6yDyeN6~DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 5EVNT 6EVNT6EVNT6$FTab'>FVoc'-FWO_GATCFeat//&GTyp!!6CHCFGCEHCFGHCFGHCFG==InScInVtLANE$LAST~~ZLIMS!!6LNTD2LsrPaMCHNMODF6MODL3730NAVGNLNE`NOISpP1AMMP1RLM P1WDM?P2AMM sP2BAMM P2RLMPBASMMPBASMMأPCONMMPCONMMqPDMF\PDMF؇PLOCMPLOCM=PSZE`PTYPPPXLBRGNmPtRMXV1RMdNHPRMdV1RMdXH`RPrNPBRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT  -)RUNT  Rate 67RunN""PRS/N%hSCAN@SMED SMLtSMPL!![SPACMSPAC؀SPACMSVER4.0SVER SVER,,5Scal@Scan@TUBEG6Tmpr<UserphARphCH؞phDYbigphQLcphTRphTR4{,|Qv%_F}K7T]Zj7y!R}|MCZ9zvr3H}T"mqD-WeDmq$a~8;R/;8-b#DI#k>4L\\W'KFxz2jA&owbor~Mu^yu6v;(vJ4;iD4ZI,nG9T+8@/Sco6m~2HBI."#9?yv`R& T5E0ml{W7;rRU)>`V2]<|'kp?_sDk`457^^s?92>@iG-@,P> u)v/|vu~CM#Y\Vd*U5aO/CsINi@C,{s2fo;LY&S2Bjr<|Ate1}1"/"@-bWF4oFo;X.: wT_fuwQEnfD~VJ(/on}sF{AR8=(V-OGC#!^MrrG]#Q<7B)L"i;k+}t#26$2!dF5A8o/4wL+}2o~*,p""#]eq }X'KD7I 9_[*?bCDkSOj!Q4M"}JMzR9JK[Yl)d762H#A#Q9U,Z:X=<;q4n%DSi^tpuze2#Xxiy?,1OPh~wA[h@tC]"#]}l~e,SXtmI^#lW4?/D:Q64Pot pN9IlVOY(xs/b@j/.=y`6@{u4f\TP/h;ZQkwqzFGP7T]V%R5;MmN2CgGz^nLoT.iPtj glX ,cW`e;E;qK|-Vz^b;0|>kij7!}WQ`QEJ9."G>}O!:i1At*ldAT/n@6TdO{7+VDSHM6FNmMOx^.EW<#G@njH[=K{_"#k6l2WeH?W~jHe(VZ#qKGG;1m.Y.PhkWgw/|9vAMV^@Yo]p&qn~+lU,YTl_{1#p{XPTF}cyy3of)`|!-Vt2`\wmj773Ku\@\=5NDzU4oD7N**s$[-33.BkS7> YlGcRb[xy8J9tG>udrV=y`6@WBktK3c*6?FYT7~U+U*rR_"qaf/h7kZ5UPh_,zi8*rInqsOUDzjX%Z=N*+WDdA7+gsc\Ny|7OU+NU[xinJilZbCd]'p8Npdpu#Wc4hAC?-4$&FkNp&iJ-omMOxjYa/k#Pb~;H26t{_"#kI>b0FcS==6X12Dg8YauBQBhwD~EG7&o.janJiRzq`6\qwHN02T(vW3TA=]Ik'2g|ea}wKShKn6g;X6YM%gcmeY_@h.<$.^ka@GpSC>lVm? ##k0$y!TW3nG6`VX+*flVOY(xs/b@j/.cgoC#Q9UP_`G\U5ZfoQe=N^tpu&ofW)NKo-j2Z.bB E:Yh^n@EK5llmeDATA /_LDATA /_yZDCHTDSamDySN 6dDye#DyeN6oDyeN6tDyeN6yDyeN6~DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 5EVNT 6EVNT6EVNT6$FTab'>FVoc'-FWO_GATCFeat//&GTyp!!6CHCFGCEHCFGHCFGHCFG==InScInVtLANE$LAST~~ZLIMS!!6LNTD2LsrPaMCHNMODF6MODL3730NAVGNLNE`NOISpP1AMMP1RLM P1WDM?P2AMM sP2BAMM P2RLMPBASMMPBASMMأPCONMMPCONMMqPDMF\PDMF؇PLOCMPLOCM=PSZE`PTYPPPXLBRGNmPtRMXV1RMdNHPRMdV1RMdXH`RPrNPBRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT  -)RUNT  Rate 67RunN""PRS/N%hSCAN@SMED SMLtSMPL!![SPACMSPAC؀SPACMSVER4.0SVER SVER,,5Scal@Scan@TUBEG6Tmpr<UserphARphCH؞phDYbigphQLcphTRphTR