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!!!"""####$$$%%%&&&&'''(((())))***++++,,,,----....////0000011112222333334444555556666677777788888999999999::::::::::;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 5c60a0ef4a4346a9ace40aaa2506e76cSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-28_09-06_06723130Seq16716134697_134697mfagan96-WellK1313_G4_Piper_sp_matK-xFKB_3730_POP7_BDTv3.mobSeqPop7zlqxtghwtZE85<K\ltsj[J<1*'')++("&4;8,     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"                                                                           .xh@A>AO'A҉KB.bcpKB_3730_POP7_BDTv3.mobtermAGAATAATTTGGAGGCATATATTCTATTTATATCATATATCGGATGTATTAATACCGTACCCTATTCATCCAGAAATTTTAGTTCAAACCCTTCGTTACTGGATACAAGACGTTCCCTCTTTGCATTTATTGCGAATCTTTTTATACGAGTATTGTCATTCGGGCAGTTTCATTAGAAAAGAAATTCTTTTCTTTTTCAAAAGGAAAATGGAAGATAACCTTATTCATATATATTCTCATGTATTGATGGGATCTGTTTCCTTTTATCGTAAACATTTCCATTTACGGAAATCCGGGAGCCCTTTTGAAGAGGCATTCTATGGAAATAAAAATCTGGAGAATGCTTGAATGTTTTCGAAGGCTTGGGGTTGTCAAGACCTTCAGATAAGGGAGAACAGGGAAAAACTTGATTCAAGGGACGATCTTTGTGAGAAAGGAAAAAGCATTATCCTTATGGGAGGGATTTTAGTGGGGTCTCACGCCCAGACCAGGAACAATAAGAGACCGTCCTCAATATGGGCCAGGTCAGGGGAGAAAACCCTCCAGAATAATTTGGAGGCATATATTCTATTTATATCATATATCGGATGTATTAATACCGTACCCTATTCATCCAGAAATTTTAGTTCAAACCCTTCGTTACTGGATACAAGACGTTCCCTCTTTGCATTTATTGCGAATCTTTTTATACGAGTATTGTCATTCGGGCAGTTTCATTAGAAAAGAAATTCTTTTCTTTTTCAAAAGGAAAATGGAAGATAACCTTATTCATATATATTCTCATGTATTGATGGGATCTGTTTCCTTTTATCGTAAACATTTCCATTTACGGAAATCCGGGAGCCCTTTTGAAGAGGCATTCTATGGAAATAAAAATCTGGAGAATGCTTGAATGTTTTCGAAGGCTTGGGGTTGTCAAGACCTTCAGATAAGGGAGAACAGGGAAAAACTTGATTCAAGGGACGATCTTTGTGAGAAAGGAAAAAGCATTATCCTTATGGGAGGGATTTTAGTGGGGTCTCACGCCCAGACCAGGAACAATAAGAGACCGTCCTCAATATGGGCCAGGTCAGGGGAGAAAACCCTCC#-4?Rcy -7CL[hs| .7BJXcoy (2?LSbmx'2?LYbmx(4?JUbmx '4=IU`kv%1>IWajt,8DMWdnz   1 = D O [ e r y   , 5 = I S ] k {  , 5 A P \ j u    $ 5 D N [ f r ~    ( 9 K \ q | $/=HWapz %7DS_fu)6HUam{(/=DQ\jz$3FSZju| #:DSky &.>MXdr(3>Rblu*6BJZiy $0:EVkt|$1=KXbq| .?L[hu #.#-4?Rcy -7CL[hs| .7BJXcoy (2?LSbmx'2?LYbmx(4?JUbmx '4=IU`kv%1>IWajt,8DMWdnz   1 = D O [ e r y   , 5 = I S ] k {  , 5 A P \ j u    $ 5 D N [ f r ~    ( 9 K \ q | $/=HWapz %7DS_fu)6HUam{(/=DQ\jz$3FSZju| #:DSky &.>MXdr(3>Rblu*6BJZiy $0:EVkt|$1=KXbq| .?L[hu #.    1  ).4   '8 &/3)',388&;,8 ;33) >>86&'>$ 6+0.;;'1;&$!>; ;+ 8;. 833.$0.-",0""2660         "                                                                                          1  ).4   '8 &/3)',388&;,8 ;33) >>86&'>$ 6+0.;;'1;&$!>; ;+ 8;. 833.$0.-",0""2660         "                                                                                       :N%OMF;= 4\<G CRW Q+LG39WJS6D4#M*RILI&L G<X]>> ? MX2DMZ_OB(E cNV]]#RA:+]3O?0+HMS-R1&O"(6F6W#G%.&ZL%&--"WAN;`7$;9UcTGK7AH8+M/O290) E;$M0H-BC1ZV@ .J@>c c.AX;ULQG\[R#b_;'X4%9Q *%:6H1 #]5]U>IJDJ_.0+[?AJCB7@IQ ]'86U?@*S](PE*C<Q8I]LQ!2* c XHOJ2AZ > ]7` DcVP! ! ?'C"G1OM7ZaFbM;4\X1I*BCS$@``]PCSBQ 2 c.)!/B*QFFJ<'HA=+S[*45%,**NL9P/$[ I!:7GO!^A"D1bX c[+,4FUJ9Ha 3 $TJZ9_;H%7FR%EM.WH/V)/Q?$1AK#B[)Z) U 9V %@U>J%QW<a>:Q3,HE8>4J/Q%< ""93'~G 9H8^Ph$aFpjbeZLLy6 =dNNiO:<*B0cbFr{/$+i6`^(0fL;%&sx (+r2N^{|6] w9i 8.#\x"5D|2'W"u0%2iGvrT[%z=r XCrg$3OZ%F4 ?0bsRixAvZ$qwe B~ E ?.#~TU"2C18v4s cX(qU\Ikj<p#VCNP?X !-SBb+G 5i`se C;wQo)m,Cq$uX'J<qd@rY-e>,D$*RE)*8S ]JVxxrOkxtj_YeSw@O[Y7J2+BN5J.z)g0:Q.TE"BphU%6YCMHiWFEc;B1!bK^I?-Ad8j*NY*4$96Dd_:J.- : e5=G} 6Ks9"6ul~J bYF418W`;y.3@6JR`ql{81Qd'C].M4P[@L.U [LI!:7d!El%;Z#;<.R<au-?bcJ73$Mu< l1< %*+\47F.P+,P]$>ui>[&Jd]T :AwG$]A%-$7"80aaO6OjboTu7$* 1|`19"=9)R U>-S)F!A?RmecO l-&4 y&?7j["ncp+pppW ``TA>W:pXpILi+>1,UxXbHZQz 0+GpC[*$AQo"CcN=,8g9-"i1 ;:nCp |,O!_@Hs>2N=Y8,pZndGh>tOCLLA?@pe)8K(.gyp tGw.,v>pwj5mhp*RpG@%:6\z 4z|p]f>mVA-E8g K ~i 7^ CL5nwgQ?l_lEe<j<[X[YG!qwLCKF:G4gfS hWMaeiV[zV:-SW42lb {WNW}XUQ~xdqu@/DckP6\ =JcM^ |y#D y!VZ *5  V+uWyanrttCvlGxezH-S= IahpG$6;9T;!] MN)BT"jj]RN\\/ &\ #,N3M`$o,W` QMZrIznT(,FC6]i|&"MC7P$m4{;dzk)6@o!Qs^R|GATTCTTACCTTGACACATATAGTCTACATATAACATATAGAATATGTAACTAGATAGCAATCCAAACAGACCGGTAAAATTGCACTCTTGCCTTGCCACCCTACACGCCATGCTTGGTGGATGCCCCTAACGAGTATCAACCTATTCGTGTAGTGGTAATCCCGCAGGATCAAGCCTGGACTTACTCCACTCAACCTTTCCAAGGGTATAGGATATTGGCATACTCATATCGAGGCTTATGTAAAGAAAATATCTAACTTACGGTAGCGTGCATCCCTTGAAACGATCTTCGTATAGAGGACCCACGCCGATTTCGTCTAACGGGAGGGCCATCTTCTTGGCTGATTTAAAAACACGGTCTGCTTTCGATGACGATTGCCATACGCGATTGATTTTTTTTTTTCTCGTTGGGCGAAACATGATCCCAATGAGGTTCGGTTTTACCCTCTGGGTGACCGTTTGACGCCTCTTTTCTCACGCAAGGTGGAGATTTACGAGGATTTTGTCGTCACTGTGTTTGAGCCATCATTACCGGTTTTTATGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-28 11:09:44 -04:00AEPt@AEPt@APFN \ APXV2APrN 6APrV1APrX}}6ARTNASPFASPtASPtB1PtB1PtBCTSBufT PPCMNT 6CTIDP|CTNMPCTOwPCTTL 6CpEPDATA@DATA@DATA@DATA@DATA PDATA PDATA P-DATA P*}DATA /_\)DATA /_7DATA /_EDATA /_ySDCHTDSamDySN 6dDye#DyeN6oDyeN6tDyeN6yDyeN6~DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 5EVNT 6EVNT6EVNT6$FWO_GATCGTyp!!6CHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!6LNTD2LsrPaMCHNMODF6MODL3730NAVGNLNE`NOISiP1AM @P1RL @P1WD @P2AM @P2BA  P2RL @\PBAS  ڼPBAS  ؜PCON  |PCON  \PDMF\ PDMF؀PLOC @PLOC @PSZE`PTYPPPXLBRGNmPtRMXV1RMdNHPRMdV1RMdXH`RPrNPBRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT  -)RUNT  Rate 67RunN""PRS/N%aSCAN@SMED SMLtSMPL[SPACMSPACySPACMSVER4.0SVER zSVER,,5Scal@Scan@TUBEG4Tmpr<UserphARphCHؗphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN \ APXV2APrN 6APrV1APrX}}6ARTNASPFASPtASPtB1PtB1PtBCTSBufT PPCMNT 6CTIDP|CTNMPCTOwPCTTL 6CpEPDATA@DATA@DATA@DATA@DATA PDATA PDATA P-DATA P*}DATA /_\)DATA /_7DATA /_EDATA /_ySDCHTDSamDySN 6dDye#DyeN6oDyeN6tDyeN6yDyeN6~DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 5EVNT 6EVNT6EVNT6$FTabFVocFWO_GATCFeat..GTyp!!6CHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!6LNTD2LsrPaMCHNMODF6MODL3730NAVGNLNE`NOISiP1AM @P1RL @P1WD @P2AM @P2BA  P2RL @\PBAS  ڼPBAS  ؜PCON  |PCON  \PDMF\ PDMF؀PLOC @PLOC @PSZE`PTYPPPXLBRGNmPtRMXV1RMdNHPRMdV1RMdXH`RPrNPBRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT  -)RUNT  Rate 67RunN""PRS/N%aSCAN@SMED SMLtSMPL[SPACMSPACySPACMSVER4.0SVER zSVER,,5Scal@Scan@TUBEG4Tmpr<UserphARphCHؗphDYbigphQLcphTRphTRU[lWQXb^kOptpuD:\E+mHY`/&mayX9'-vyNJRA=57AMjCW`D/z\|DX&_lp2]m"]P"F77sI_6sKGol83p{ ]GxNu~)B*HUrG\W\lYT`M=4T*R7R8~f)NK[>Id`Po<_+MWQ}VX*oE}`Z&oRLV;);-ags5)9mW'>YP~jhM^,%bkb |{cokB}qHqHStXK#XTE-=[c}Sn2M5FiK&=RSbXW,62DmOYX/D?**s$[-p+D42F7f6QuA!9@1%ilJG82G [7P^E$62:gt]pup8$(}fe0&TiY*R2wt)jdd08o8:"]R_s_be1}1"/"E.Om,P>bH6X12g[jWcgiQF4Np3`4 x'gJx%*{#ODYnWZjdO{7vMjL3ro{`N%7`h?I8rBArIUwch[}k@_a'>yj6m}P,PHq S]*Cy KJFU'.Q1db{wMzR9v!w2l89+uTE#!#hmmfc4hACTT3T&^%FyPC,61h67{=Y`gI)_|_n?NhN{CwN1d^MT9HUsg9T[1reHuMrkc[j]/7<("Hf.Q*s`AtGHrd+v/,J6v$NUvc*xGZ]k'lCKOMQwKdLC2fo;LY&f&){t~1{[q?Eb,8`a)}qjM>PCNIG2gc*?7kx`rxPo"`dbB-8'i2{q6.>`"/OCf7uihukWy4=?6vtfQgsk>H~pL"A~oTq}}89u"HQ\Vxzhh{!U)P? sIRD8z{KJ[.z^`A(uKN73B!Hiw8X&L>o.B3@dSO/qg616B4#ky`[qDr_WG\|[S*.JJof6[[HhEg5{og]|_}e])%O1"@7hhSc%bVm? : wT_RuhGlB^PY6C}Afz@jXaMuF_QJO!GE#!#hmmfc4.=+I}S$oa$fU~oM6CqwToN668d#