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!!!+++444<=<EEEMMMVUU^]]eeemlltssz{z !!!"""####$$$%%%&&&&'''(((())))***++++,,,,----....////0000011112222333334444555556666677777788888999999999::::::::::;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 6d6206d6e6664d66811295ff2873c8baSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-28_09-06_06723130Seq16716134697_134697mfagan96-Well!K1313_G12_Cobaeae_paneroi_matK-xFKB_3730_POP7_BDTv3.mobSeqPop7z #Fp*Tx$V %9W{oTGJYl{~t[7ycI, xHfPOf  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(;(&S/UK-ECO`3*A'OSG;J+5&?D?2=*DKIG">GD8b\>4/`OQ(M_C-'23R[,,1ETZK><J08X(\U;(L<D]F:0#T$(&V]1[S"Q;8LKc'/ObKMS (@B 4J *21Ra2 TG0cJ&I# H =DR@051GM,%'c@?M,UJ.;4SB @ : K<5/F2*<P0YT(0,(XQIGA;!%?.R6^%,P4[%DU5I8X:^%C!bbG_%?bJ0#2AXBWJW4)/5;A8T-)/D(1A&5$;FW3(2Q2UV.EWM:$^Y D<(2\7-2I`2?`ZCb@G @E2  2& ;(9:^&J2[ b@F2 XNa2M9#U#(-2@cQPE#8T0UHbIP+GA<2M?P<(P2!I2(2dZP<(3P<J(SCE!I!C#2AB fJ O _E G (_$_ GYXzRN|5r4$nj y&_/77auA,M.P:%  x0w   sSWN5y+!_ a3M<{%|NxlGctM ZIqdxUXtwlHX6YnRyG!>;MXnhewJ%lXq^b&3+h8i3S7@+W1y:^#_}*ABj*6\WKC~B5$}5 ;88%%F, g6ieJ4~]pf<G6\($ ptgU@Uw3=1 V!bzZC3nJv_?1(YK<I$!%q Zq=%&7r/\^Rktz;@"P/Nm"rgA/R.FnL-e=9=]"4 z}%6/ajZL!'i W?6E+H]ax%Wc(g$FBEd-J cC\%u'wH"8ZtVF\}|  Q(9R.ZAI;-yyVWkC /'>:}1h1S 9uGA(eeS!'  iPkx^   p 6?,9 G!aqwb|/zpWndoA%bXNN]eRPD_cWOTdSj[EnLN@\xy`yahzriyt *x6ZPqezxjbau@k?|:uQ3YU,db_VZ0-W^z$3xp"{cyQ]@e~c]8|v f^riu%jP2D|MY-=>PI1i&Ph}YIOrd7g UqY{Fgq L<b!s vS7*:(8NbyfQ@xg& T(y+By .s|dz lW+aN[AUGO Y]>E BZcg5X[y89WWl#>~8j: QQ QQ      QQ Q QQ   QQQQQ  QQOOO88O8OOOOOO88OOOOOCcCCcCCcCCCcccCCc)cccCR         R  R      RRR          R    R    ))OO))O))OOO)|)|))))OO|||)))OO))O||)))(|||)OOO=))|||||||)))O))||||Y|)|C))))))))|))))O|O\|ppF/!"M,p]ppwWc p 4IEKdp|p^*/pp<v7!G8Nkp%AM.C-W+_Hhqip]Z;2S7s_Rkty"(G8aBk8G8Mdu8L S<%X{+ 6^E$<=Yed8w6_waHC[786(,n#/bBWufp1?( v|p8j/Sr~pRB0}P(<V,B,oP,d402"fHQdD]Qpx{]%.X@Dq 0,pypp k|Y,,DppQp8ghm|dxB8QCgU7k*,~BT,dU 5i\ 1 ==Px@lj<!*IKI-)pv2J*8F~5Myh_ \ v:{[ ca1"eGnX&TZI(AGH 0Y E,;lzv#3 <cZfZ1J%zU,>PbEtDn")XO>r'x/YxEx4B407T}I(? |>u).qymm[|N6"q;S~dygGA,}0vQ@ U 9Z*nOZi4MY@p@{~u`&>@4) 1O9ZzWW-TgRaO|x2;cDWOAqw^)a FGzr01blenp  :|+YldSpV;R KVyb@LesqK LXI`Qj&;jt JB M|1IOl ~~]Dub SG3]uAeBb4qA`M5E g|tvJ2r*`nY:ar6zL,\[Mx6}AATTTTCCTTATTGACATATGATATACGCCCATTAGGGCGCGATGGATATACGAGAGGAATAGCAACATATCCCTCACTTAATAGTGTAACGTACAAGATGCGAGCCTGGGGAATACGACTCGTCGACGTACGTAATAATAATCAATCTACGCGTATGGAGTGGGACCTATTAGATGAATACCTGGCTAATCGTGCCAATCCCCCAGGGGAGGTATAGAGAGAAAAACTGGCTCTGATTGGCTTCGTACCCTACGAGATAGGCGCTAACTCCCGCCGAGATATAATGGGCCTCCGTATCATCCGTAAAGGAGCCACCTCGGGTCTTCGGGTAATCTCAGCGAGGGTCATTGGAATTTCTCAGTAGTCACCGATGACAATTTCCAACAATTTATTTTCTTATTTATCTAAATCTGTTGAAACGATGTTCCCGGAGTTGATAGTATCTATGTTAGTCCTTACGGTTTATCCATAGTTTTTACACACAGCATGCGTATGAGAGAATTTTGTGTTTGTCGAGACACCGATGTATGGCACATTTTCGGGTTTGTGTTCACACTACGTTTCTTTATGTGGGCTAAGTTAACTCGGGATATAACCATAGGGGGTCGCGTAAAATTGCGAGAATATTTTCTTCTATCTAGTCTTAGACCGGTAGAGGCACTTTATAACCTTAGTTGTACCGACGTCTTCGGGGAATATCATATTTGGAAAGAAAGAAGCCCGGATAAGATAGGTCGCTTCCAGTAAATCGGGCCGCCTTGGCCCCTATGAGTAAGAGTGGTTGTCTCCGATCATAAGGAGAAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-28 11:10:54 -04:00AEPt@AEPt@APFN \(APXV2APrN 6APrV1APrX}}6ARTNASPFASPt ASPt B1Pt B1Pt BCTSBufT PPCMNT 6CTIDP|CTNMPCTOwPCTTL 6CpEPDATA@DATA@DATA@DATA@DATA PDATA PDATA P-DATA P*}DATA /B^\1DATA /B^DATA /B^9DATA /B^wDCHTDSamDySN 6dDye#DyeN6oDyeN6tDyeN6yDyeN6~DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 5EVNT 6EVNT6EVNT6$FWO_GATCGTyp!!6CHCFGCEHCFGHCFGHCFG==InScInVtLANETLAST~~;LIMS!!6LNTD2LsrPaMCHNMODF6MODL3730NAVGNLNE`NOISIP1AM%JP1RL%JP1WD%J8P2AM%JP2BA%%P2RL%JPBAS%%١PBAS%%|PCON%%PCON%%ZPDMF\PDMF`PLOC%JPLOC%JPSZE`PTYPPPXLBRGNmPtRMXV1RMdNHPRMdV1RMdXH`RPrNPBRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT  -)RUNT  Rate 67RunN""PRS/N%ASCAN@SMED SMLtSMPL""[SPACMSPACYSPACMSVER4.0SVER SVER,,5Scal@Scan@TUBEG12Tmpr<UserphARphCHwphDYbigphQLcphTRphTRWThis is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN \(APXV2APrN 6APrV1APrX}}6ARTNASPFASPt ASPt B1Pt B1Pt BCTSBufT PPCMNT 6CTIDP|CTNMPCTOwPCTTL 6CpEPDATA@DATA@DATA@DATA@DATA PDATA PDATA P-DATA P*}DATA /B^\1DATA /B^DATA /B^9DATA /B^wDCHTDSamDySN 6dDye#DyeN6oDyeN6tDyeN6yDyeN6~DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 5EVNT 6EVNT6EVNT6$FTab"FVoc" FWO_GATCFeat..!GTyp!!6CHCFGCEHCFGHCFGHCFG==InScInVtLANETLAST~~;LIMS!!6LNTD2LsrPaMCHNMODF6MODL3730NAVGNLNE`NOISIP1AM%JP1RL%JP1WD%J8P2AM%JP2BA%%P2RL%JPBAS%%١PBAS%%|PCON%%PCON%%ZPDMF\PDMF`PLOC%JPLOC%JPSZE`PTYPPPXLBRGNmPtRMXV1RMdNHPRMdV1RMdXH`RPrNPBRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT  -)RUNT  Rate 67RunN""PRS/N%ASCAN@SMED SMLtSMPL""[SPACMSPACYSPACMSVER4.0SVER SVER,,5Scal@Scan@TUBEG12Tmpr<UserphARphCHwphDYbigphQLcphTRphTROp]C$xX+lkfc40-fKbVUg|=a1tirD:Z5A>9^kZdg~WI|Mb;eX}"if32#/P[~W!Fy^q3Th$Y i ^UO ]ddc}?8d)sGi=&MQJ1r]ZvZ~h6dYHa?yh;}S$PSD`^BnBCmJ1y;MmN2EmIz^{5te.ERbE2(5?fqcdsTF]lOTz9NKA\@:AV^V4?HV;mUCBM[?|$}d& 'iJ4~CubW_]?ZC'T3T&#ONx8LkV}d7?#)io5`N0W`,#Xr_<.Fi1A+7x,"L-2QvA- d+[Y.me|[{0%4dc{Ae`ox%CR"lyV?:w02"_pgr:vrG\W\lWQ7M3gptQ>?";!8L[2Rr`qHT^1R=goQS-CDiyX^UM]N=?W.ss$T!D@4ir$Gj~`? MlYXjlnK[Yl`A #pJE,#VuU4zrfv4&Aza/.9{|mnVNW+eD>0N}5!){mnjH[v]Eb,8HW>ycPJ~FT,{1:Kn6?_s2n`457^^sIdwz@{&]t[X'(A(f'PJA}*4d#kE_GY@ZWeoPTF}cyR6A;{IF}o-jAS_7ea_';_/Ft(#j1DjU5]DtmXY#agsC!.:QI5&.XRz+*}Lq&Wi1Cf]x@4}@i\q^*u&XH4l_rDXW"&dEg3 dkIW~L?n[Y<,h`\wmj7a;A{%i6#a)lmeudrVLgJRv)1mfc4hApvaUGxP=O_l;mqy=Y`ge#cme/^i1>QM/PN6M|J#2Lv'PH[R)O1M]^2nrD5pY-7cRb[8)FTM~j3XV=K35-x>;u4,ZBE=+HqdszeOf^DR!_T6#qLQ6?ZdwYc_`kc.}O!p8Npdpu#Wc4hAZG|JWIKT+}tr2LxCmqL}4|Mde_'61Qve])%,8=0?})jZ>zir8rP p'Ic62ir$1.XH`HO5QqE`|mwP@rB ]:]HE^OXv&nP_[O~}<"&eG1h3/[zGq;)y6}c8dgp3^|o:Xz zFA\|5DhECel7B]%jxxsWD@xG57'9\47./D?*&qDp-o*Cwh1nk-~4Kb}wLD7X{Mf4-gAEPt@AEPt@APFN \(APXV2APrN 6APrV1APrX}}6ARTNASPFASPt ASPt AUDT01B1Pt B1Pt BCTSBufT PPCMNT 6CTIDP|CTNMPCTOwPCTTL 6CpEPDATA@DATA@DATA@DATA@DATA PDATA PDATA P-DATA P*}DATA /B^\1DATA /B^DATA /B^9DATA /B^wDCHTDSamDySN 6dDye#DyeN6oDyeN6tDyeN6yDyeN6~DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 5EVNT 6EVNT6EVNT6$FTab"FVoc" FWO_GATCFeat..!GTyp!!6CHCFGCEHCFGHCFGHCFG==InScInVtLANETLAST~~;LIMS!!6LNTD2LsrPaMCHNMODF6MODL3730NAVGNLNE`NOISIP1AM%JP1RL%JP1WD%J8P2AM%JP2BA%%P2RL%JPBAS%%١PBAS%%|PCON%%PCON%%ZPDMF\PDMF`PLOC%JPLOC%JPSZE`PTYPPPXLBRGNmPtRMXV1RMdNHPRMdV1RMdXH`RPrNPBRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT  -)RUNT  Rate 67RunN""PRS/N%ASCAN@SMED SMLtSMPL""[SPACMSPACYSPACMSVER4.0SVER SVER,,5Scal@Scan@TUBEG12Tmpr<UserphARphCHwphDYbigphQLcphTRphTR