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"?       d,,,III^^^{{{&&&///DDDNNNaaavkv      ***333>>>GGGGGGRGGGGGGRGRGRRGGGRRRRRGRRRGRRRRGGRRGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRGRRRRRRR;SSSKKKiii !!!+++444<=<EEEMMMVUU^]]eeemlltssz{z !!!"""####$$$%%%&&&&'''(((())))***++++,,,,----....////0000011112222333334444555556666677777788888999999999::::::::::;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: ae068c3622634ee5a5ff9897675eaf25Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-28_09-06_06723130Seq16716134697_134697mfagan96-WellK1313_G11_Eugenia_sp_matK-xFKB_3730_POP7_BDTv3.mobSeqPop7z(*-049@HQY][O<& $5@D@80,/5<?;1$#".E[lrmaSHDIWj~|}1^ *Hj06,yO'kL,xiXKEIS]a\PC8322/&   #)07>DIQ^s !/D^vxcPDCKWgy :cfD& mI#tmouvmZA+ ,>LRN@/!".:CIKJJJJHD;0#  #&$%5IZfhaTB1"#:` Av';7g.rXE;8994+  />KSTNB1  D{ K{{aF) vi\N@4*%%*2:<7+ "#    Ev>dXoK3# E4|sX4L'#++#  $P:={@!wV6  @{-!8'VgJ3  !(*& #*(  !/63) !3MkH@?6nK+ (+&           '+' %)&   9Z~z^H90,($     4Lkck"}fbo{^H:2,)'&'&#&.1,!      $%$!  9Rfqvy~X3     8Z3=-V3+;\xpliffhmrtn_H. !-456=OnpJ- ! !;OVN9  &3CSdsmV=$ $))#   #175* ! )Mty]?$ !+243.'    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"           #                                      `>T^1C #VD8/8`Z3DDA'1R6X;K% FI@I/PbKW"STK.,#K-J H "OD;[O?I^5@bJH C-Z!#0+F/$<]LT0'74/KD8E'R8)WM4K`(;P_O8%]0X#="ZR> ?%S.F " b#CK.R]LAH2F"Bb`F9+%!@d$@$HG$2%<+O% C?%b?>,^E ? %2MD?338Z!E0 $;[;V)8` D)\ ;)5H;2M2)J3)2)H>;)*742-_Z*,P6a'Q=FW.2N*:5*:G*J#2*;O*T^*Q*:2S*#:P:*:*2`** ' p$D[PG3QuM{/>*c/z%GEp:ZP~FMh;ft 6/6'E | $#v>t(eb-2^:m1C]Dw=p5Wg;P FzdhT r^4l?} ?O {rTB -\o=h)ltS5u'&a +,bGiiXr.I'_aRp T^uo/tG\m?V)-\53d.zX*@]_Ik,aMrw,Aw /DxosT+F7{4VdX|}etZff8 S2xiM_NpHmgl_>X<<d6"yM0 KL?"g_NLdr^*$ %&7ZNjHu?S-^&Ud67&Jjm/B x&/epS?5,0+~|P01p0(!e&XP 8a 88$*~J8=a@D4p]mR8]kp#Mxp(?/]8L;#j/%pA=h*q7  f, .#8 ,fb  !|j_sUp\3p=:]*~b f#P=p Xl c4TDgiv&}Vr7ZdTiDyd]xOq`[[G{kR5l9}V/U>Nqxf} g2L4G1xRl}5#@^)7\$|+C{M`c]!5Y U, ;m9M6$jK+*D;|"Q0TtTO_LW.=V#Sn]%E}SXA8-++3BS|fzDn$!6x@6JTt/mi:>R!P[+tGGGGTTCTTCTAAATAGAACTAAAGGCGTACATATAATATGTGAAATATGTACCTGCGTACCCACACAAACAGATCGGTATCGTTGGACCAGAAGGCTATACCCAGAGGAACAGCTCCGGGATGGACCTAGCGTCCTCAAACTAACGAGTAGCAGTTGAGTCCTAGACGATGACGGTTAATTCGAGGCACACGCCCTCGGTTGAGAATAATATTGTGATGGATATGACAGGCTTGTGAGTGCGGACGGGTAACTATCAGCTGGGGCTGGCTAGGACTGTCCGCATGAGGAAGACCATCTGCTTTGCATGGAGGAGCGGGATACGGGTATAGGCCGTGAGGGAAAACCAAGGGGTCGTTAGTGGGTAATGGGCAABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-28 11:10:53 -04:00AEPt@AEPt@APFN \#APXV2APrN 6APrV1APrX}}6ARTNASPFASPtASPtB1PtB1PtBCTSBufT PPCMNT 6CTIDP|CTNMPCTOwPCTTL 6CpEPDATA@DATA@DATA@DATA@DATA PDATA PDATA P-DATA P*}DATA /]^\,DATA /]^DATA /]^DATA /]^xZDCHTDSamDySN 6dDye#DyeN6oDyeN6tDyeN6yDyeN6~DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 5EVNT 6EVNT6EVNT6$FWO_GATCGTyp!!6CHCFGCEHCFGHCFGHCFG==InScInVtLANESLAST~~LIMS!!6LNTD2LsrPaMCHNMODF6MODL3730NAVGNLNE`NOISP1AMvP1RLvP1WDvP2AMvP2BAvvP2RLvPBASvvPBASvvOPCONvvPCONvvPDMF\ PDMF3PLOCv'PLOCv;PSZE`PTYPPPXLBRGNmPtRMXV1RMdNHPRMdV1RMdXH`RPrNPBRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT  -)RUNT  Rate 67RunN""PRS/N%SCAN@SMED SMLtSMPL[SPACMSPAC,SPACMSVER4.0SVER SVER,,5Scal@Scan@TUBEG11Tmpr<UserphARphCHJphDYbigphQLcphTRphTR/LThis is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN \#APXV2APrN 6APrV1APrX}}6ARTNASPFASPtASPtB1PtB1PtBCTSBufT PPCMNT 6CTIDP|CTNMPCTOwPCTTL 6CpEPDATA@DATA@DATA@DATA@DATA PDATA PDATA P-DATA P*}DATA /]^\,DATA /]^DATA /]^DATA /]^xZDCHTDSamDySN 6dDye#DyeN6oDyeN6tDyeN6yDyeN6~DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 5EVNT 6EVNT6EVNT6$FTabFVocFWO_GATCFeat..GTyp!!6CHCFGCEHCFGHCFGHCFG==InScInVtLANESLAST~~LIMS!!6LNTD2LsrPaMCHNMODF6MODL3730NAVGNLNE`NOISP1AMvP1RLvP1WDvP2AMvP2BAvvP2RLvPBASvvPBASvvOPCONvvPCONvvPDMF\ PDMF3PLOCv'PLOCv;PSZE`PTYPPPXLBRGNmPtRMXV1RMdNHPRMdV1RMdXH`RPrNPBRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT  -)RUNT  Rate 67RunN""PRS/N%SCAN@SMED SMLtSMPL[SPACMSPAC,SPACMSVER4.0SVER SVER,,5Scal@Scan@TUBEG11Tmpr<UserphARphCHJphDYbigphQLcphTRphTR0ZNch>st_@U])%fD1,;0{U[%~#mXYV<{#-$>]/mU>p>X|.8As*5.@h23|=&u@B\$nq%StumfXr9lKnD8Y~Csk%pM_T![sGj`X0?888g2lI>iV=1SbNfPZ2V~oLSHmR/+3p+M*JgbUA6WJ?eee1K/kx=gc1/kdSPaH~Cd*9+cUV&=o?eUKGM5J_32&L"gr[&Xo-,(B~bjOe:=fQSWMMd^ *7G[kgYTbLINI{$^(R[mPo1Cd}FxQPw#vpkMSvYTEl^Z-I-{0vTNiBx["#\+?%cc/99Jhp_nQoR<^II^kx^Ctc|[W(F}z2g[jWcg;Z6Obe1Cd}Fp82)XaM~6>udrV|gwCiOfFc':Z^j?F?hECel7B]%j'kjWceOSx+C!K}Z'BNp{ e,&o.jrm$v5GK:.\_'[wA#Q9Tc T-*<^k'nwi5'S;.xhKn6g;X6YMI){wclQ[>A{%i/"a-cZ,f*F8B,}|O[nY?KPQh3f9bzL4=@qsQ0=r$'PN`!$*l3JlO[,i#MQ9$5/qNE^O{?kJ:hAZG|JW>K-fU'C,ComMOxkYEZ&dAr_s_be,{Re\opejp~4o-D7I 95[Xv,Kho|[E,f`C:F__diM(]y"/:Boz?&qE^OXv&n7`[qD|3UveGf+Ko[!AcS#yK9YtI)YVJ|Q}.z zFTK(>VbUZeFHl{+9 nVI! 4J'BNpuaT/}}1%j-]Tsz^e9Su3]w