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!!!"""####$$$%%%&&&&'''(((())))***++++,,,,----....////0000011112222333334444555556666677777788888999999999::::::::::;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: d36d7e40743c4b43a521f75b1782c9afSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-28_09-06_06723130Seq16716134697_134697mfagan96-WellK1313_E2_Commelina_sp_matK-xFKB_3730_POP7_BDTv3.mobSeqPop7z&YtcJIxP : ( o E , r  2  r  m e ~ b  1 7{tw5+AN-=9C LT*n7*i9(@&I 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"         (*$  &&         *kW@1AAGAA KB.bcpKB_3730_POP7_BDTv3.mobtermCGGCCCCGTTCGGCTTTCCGGTCGGTCCTTCTACTGTCCGCAATTAGTATACCCACCAGACCCCTCGATTGAGACTGAGGCAAACACATCAACTTTTGGTCCGCGCGTCCTGACTTTAGTGCAGGGCGCCATGATCTGGTCGAATTCCCAGCAAGTGGGCTCTACCAGGATTCAGGCGTCTCGAGGTAAGCTGAGCCGCTGTCTGCTTCGAGGCCGGGGGGAGGAATAGGGAGAAGGGATAAAACAGCTGGTAAATGCTCGACATGAATGGACCACGCGTGACGCGGTCGTGAGGATGGGGCGGTAGGGGGGAGCGGCCTAGAATGGCAGAGCTCGGGGGCAGCGGGAGAGGGATGCGCGGAGGAAATAAGGAAAGGCCGGCCCCGTTCGGCTTTCCGGTCGGTCCTTCTACTGTCCGCAATTAGTATACCCACCAGACCCCTCGATTGAGACTGAGGCAAACACATCAACTTTTGGTCCGCGCGTCCTGACTTTAGTGCAGGGCGCCATGATCTGGTCGAATTCCCAGCAAGTGGGCTCTACCAGGATTCAGGCGTCTCGAGGTAAGCTGAGCCGCTGTCTGCTTCGAGGCCGGGGGGAGGAATAGGGAGAAGGGATAAAACAGCTGGTAAATGCTCGACATGAATGGACCACGCGTGACGCGGTCGTGAGGATGGGGCGGTAGGGGGGAGCGGCCTAGAATGGCAGAGCTCGGGGGCAGCGGGAGAGGGATGCGCGGAGGAAATAAGGAAAGGC'5BMVcoy #0>FT`kz'3AKW`lx 1@K\l}#.<FT]ju$3GVgu(5GXgs1<GS`iu';GS^i|   $ 3 ; N \ p y  ) 5 D L W d m z   # 0 < J [ i u    , ? M [ f s   & 7 F O \ l x  .:IU`qy );IVgt );HZl{%3?HWfx &4BKZfr)6ARa'5BMVcoy #0>FT`kz'3AKW`lx 1@K\l}#.<FT]ju$3GVgu(5GXgs1<GS`iu';GS^i|   $ 3 ; N \ p y  ) 5 D L W d m z   # 0 < J [ i u    , ? M [ f s   & 7 F O \ l x  .:IU`qy );IVgt );HZl{%3?HWfx &4BKZfr)6ARa                                                                                                                                                                               $ N/J=LPL+?A#:J&<8;A8HB2( X'7B"!4I5 @7F%LDKK6'?S,D9F)>1$<>L.?51Nb!YMQBB.J'^TU<U"-_\ <a>4$2^H./d_A?+X*XNK863 Q#X6Q=XVG8 -!U+U,$0_])Z\Y*27VMA 9$TC/?E  6?!( QMWS[6&!\[G*A@ O$*I.=>7Y#&$TX];VcE&U. JRK4 O1U KM cbU6<#`0;^ 3&F=\;8CFD*?T[ "/4'3);*< HS[ @F,b ?C]= c26>%63PDGTDG<'4Z  ayg4 ?   TO6 0W*P 4^ ht(" (/%oJf0fm>z[3BqnukVQSSQ>sj9v s/;s*1k87T%~b,(DE" =M(^/ "*&9$JOD;C?-~J%!`A_=iyPNa}u=*77cHq}2l17Z{p {3jJ:wJy4ru$nCP/SJ{.|a?Yjn ["DN~4dFjW4ly#7 ORoH36Lzc1w;Ck;C*8MB y@m:FOf "#Rfi2$H  2#%:y   ?hEpN- sppVV,t/ S=/+t$(&8kt0;(J {&g0G&c$HCIuF=( U;x43.LmMc x,-vYgD%hsVU 3D`y gXpG(9,+,dUl)vY8tEx2/} nwZsT^ 5hpKZfJXsQqNsFINk9)KVa 6;v%u   <>hC)[,:N a  $#(le1^JBj&>T,IkyQ]r \/3-OuH-s#UK6vju5n_uW$6\Mac p*0r87c:IxkUHtC*ghj| MP5S2)%NRlmh Cf8$qIp`?2_$~MAp-#x#'SO(Dq='v8<#/nhz8jG#3*s~,$N)yDWE0(:} | |mCfEGAAAAATTCCTTTTAACTATTAAATCTGCCGATACTGGTTACTACTTGGATAATCTATTCTTTTGGACCGCGTTGCTTTTGCCCTCGCAGCCTGGACTTGATAGCGTATGAATACGCTAGCGCCAAATTGTCACATGAAGTTTTCCGTTTTAGGACTTTGCAATAGGAACACGCTAACCTCTCGTCAGGCGAAGTTTTTGCATCTGCGTCTTCTTTAAATTGATTTGGTAAGCCTTCCCCTTTTTCAGGCTGCTTGCACTAGATGAGCATCCAATTTAAGCCATGTCCGTGCGACCATACCTCAGTAATTCCGCTTAATATATGGCCGGTTCGGATTTCCGCAATCTGAGATATCTATAAAGTACGGGTTTTTTTTTGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-28 11:09:30 -04:00AEPt@AEPt@APFN \$APXV2APrN 6APrV1APrX}}6ARTNASPFASPtASPtB1PtB1PtBCTS7BufT PPCMNT 6CTIDP|CTNMPCTOwPCTTL 6CpEPDATA@DATA@DATA@DATA@DATA PDATA PDATA P-DATA P*}DATA /_\-DATA /_;DATA /_IDATA /_yWDCHTDSamDySN 6dDye#DyeN6oDyeN6tDyeN6yDyeN6~DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 5EVNT 6EVNT6EVNT6$FWO_GATCGTyp!!6CHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!6LNTD2LsrPaMCHNMODF6MODL3730NAVGNLNE`NOISmP1AMzdP1RLzpP1WDzXP2AMz@P2BAzz4P2RLzLPBASzzPBASzzؠPCONzzPCONzz|PDMF\ PDMF؄PLOCzވPLOCz۔PSZE`PTYPPPXLBRGNmPtRMXV1RMdNHPRMdV1RMdXH`RPrNPBRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT  -)RUNT  Rate 67RunN""PRS/N%eSCAN@SMED SMLtSMPL[SPACMSPAC}SPACMSVER4.0SVER ,SVER,,5Scal@Scan@TUBEE2Tmpr<UserphARphCH؛phDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN \$APXV2APrN 6APrV1APrX}}6ARTNASPFASPtASPtB1PtB1PtBCTS7BufT PPCMNT 6CTIDP|CTNMPCTOwPCTTL 6CpEPDATA@DATA@DATA@DATA@DATA PDATA PDATA P-DATA P*}DATA /_\-DATA /_;DATA /_IDATA /_yWDCHTDSamDySN 6dDye#DyeN6oDyeN6tDyeN6yDyeN6~DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 5EVNT 6EVNT6EVNT6$FTabFVocFWO_GATCFeat..RGTyp!!6CHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!6LNTD2LsrPaMCHNMODF6MODL3730NAVGNLNE`NOISmP1AMzdP1RLzpP1WDzXP2AMz@P2BAzz4P2RLzLPBASzzPBASzzؠPCONzzPCONzz|PDMF\ PDMF؄PLOCzވPLOCz۔PSZE`PTYPPPXLBRGNmPtRMXV1RMdNHPRMdV1RMdXH`RPrNPBRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT  -)RUNT  Rate 67RunN""PRS/N%eSCAN@SMED SMLtSMPL[SPACMSPAC}SPACMSVER4.0SVER ,SVER,,5Scal@Scan@TUBEE2Tmpr<UserphARphCH؛phDYbigphQLcphTRphTR]nd3ghlc T-* +Wt3z{0bInTor@tbfD7E)0Ie/AK8N'nsv&'Y?sxWlZN.YRz!#fn2%FT!sz^2h3)-I5d~)Uk}`:r&xvr_WG\|8 Xo2h{/S9~i,EAx8amb#QJU0iHAj}W2yv!a!<}B]rQHfKbVU}Uz1p=|(LZjp7xMO9j;q6TB(2JLvuUlN1@#vQ!TMF4FprjDp*K0.]+0Oj!Q4aK{WlVO:}mldKV6 |fnh7{bey6INt5+y|`}__';_/A@(#jFnjUZ'KvNIGQ5ENI@[wQi^*w% enqb[*jG3T%ycX}%z/q1wv;{?kJ:hAqTFU-vbNhsrU5-uvCbbJ_<$AJu"#phI*=ASt&3Vu]f*yQGOBt_Wt01CY"IHys<7qL}4|MdwYc_`k:!.tko2KWtAcP@>/D?*Q| -4KD@4ir$G%1` b&}3672tX>VH~?p?}@8jVFTm[Po)9}P5O-!b+Smfc4P8U5ZfoQe=N^tpups'Mvn5mr+8/Yb~;HR;LJcM>(\_ 6(8bb(nJy:]@|Q$OEH:6\"B-Y@iGtX,P> u)v=i57~+lF{YTl_{1#(7GDT`h3/[zr|R5/*7ag%gcmeaooUzbaI232)O~P@abHBa+b8EY59D4udrV|gwCiOfFc':Z^j?F?hECel7B]%j'kjWceOSx+C!K}Z'BNp{ e,&o.jrm$v5GK:.\_'[wA#Q9Tc T-*<^k'nwi5'S;.xhKn6g;X6YMI){wclQ[>A{%i/"@>oW~!.eHB,}&s-@M+9mW'>YP~jM.9Wl[Z,9AEPt@AEPt@APFN \$APXV2APrN 6APrV1APrX}}6ARTNASPFASPtASPtAUDTB1PtB1PtBCTS7BufT PPCMNT 6CTIDP|CTNMPCTOwPCTTL 6CpEPDATA@DATA@DATA@DATA@DATA PDATA PDATA P-DATA P*}DATA /_\-DATA /_;DATA /_IDATA /_yWDCHTDSamDySN 6dDye#DyeN6oDyeN6tDyeN6yDyeN6~DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 5EVNT 6EVNT6EVNT6$FTabFVocFWO_GATCFeat..RGTyp!!6CHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!6LNTD2LsrPaMCHNMODF6MODL3730NAVGNLNE`NOISmP1AMzdP1RLzpP1WDzXP2AMz@P2BAzz4P2RLzLPBASzzPBASzzؠPCONzzPCONzz|PDMF\ PDMF؄PLOCzވPLOCz۔PSZE`PTYPPPXLBRGNmPtRMXV1RMdNHPRMdV1RMdXH`RPrNPBRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT  -)RUNT  Rate 67RunN""PRS/N%eSCAN@SMED SMLtSMPL[SPACMSPAC}SPACMSVER4.0SVER ,SVER,,5Scal@Scan@TUBEE2Tmpr<UserphARphCH؛phDYbigphQLcphTRphTR