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!!!"""####$$$%%%&&&&'''(((())))***++++,,,,----....////0000011112222333334444555556666677777788888999999999::::::::::;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 5f64dcf813464c2eaca6dbff44e4b4d9Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-28_09-06_06723130Seq16716134697_134697mfagan96-Well!K1313_D3_Thunbergia_alata_matK-xFKB_3730_POP7_BDTv3.mobSeqPop7zPNMMPU_kw}zgXSYi|r^J8) ,4899;?FLMI<+ 'Dd ;n*7@GLONH=->oO{ 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> G CEhO ooq|H{[C-Ohzj/;oR0bufnKkCurkU#"aV&y[X.Rt?F;,M>$q 4SP+qjh*z(BEho"N;SE=5`rYfL$HI~`C1MkJhmz\{d3R=.``gP'9$]Mjl mqDvTJ<hUy^Aohf9Tv#;r?^cFo<y7[^L@T853B7., > =So*'^3&[z85j3G& 8R$:+KFj b?2_+=nqrH=R qh;;@tZ<|e)!LN5Pk-/q"0BsIYB|O(mBy-K{^]W{YHib|*8 :@=UMEq`}c}bqxoQ8xt0jE'3W45..,$&RoMH`b96]tBO(5a*%:%0 %OM??98[B+Z&y<iR(}k!G 7P(oI7}ex$rAqFV) M='G [ fN;5.<Y)b1ta @ATTTTTCCAATTGAAAACAGGGGACACACATCTAAATTGTAACATATACACTCAGGATACATGGCCAACACGTTCGTAAAAATGCACGTACGAAACGATAGGTGAGGCTATACGACAACACGGCGGCCCTAAGAAGACTAAAACTCTTCCTGTAGCGCTAGATTTATTAACAGAACACGTCGGAGCGCGGCTGCTGAACCCCGTGATCGCACTCTAGGACGAGGAGGTATCTGATAGTAATCGTACTCCTATATTACACGGATGTATCAGATACTTAAACAAAAGCGACAATCTGCGGGAACTATGGTCACTAACTATATCTATAAACTCCTGTGGGCAGCGAGCAAGCGTCTAAATCGACAGCCTATACACTGTCATGAGAACGTCCCTCATTGTTCTGGAGTGCCACCGCAGATTTAGTTCAGTGAGCGTCTTCGGCAGTTAATATAAGATTAGCGGAGTTTTTAAATCTAGCGAGTGCAGGCGGCGCCCCTTAAGTACGATAGCTGGCACATGGTGACACAAAGTCGCATAGTAAAGTTTCCGCGCATATTTCGGAGAGTATACAATAGGCGACAATACTACGTATAAAGTACTTAAACAGCGTCACATTGCAATCAGTTTCAAGTACTACTCTAGTTACACCGGACATCATTACGTATGGGTGCAGCATTCTGGGTAATAGCTCTTGGGACAATCTTCGCGTAAGTTCCATATGCCACATGTTGCTTCCGCTACCAGATTATGTATAGATATATACGAGAGTCGATTGCGAGTCTCATCTATAATGCGCGTCTACACCCGGGAGGTTCTATGTGTTATTCTGTAATTGAATABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-28 11:09:50 -04:00AEPt@AEPt@APFN \(APXV2APrN 6APrV1APrX}}6ARTNASPFASPtASPtB1PtB1PtBCTSBufT PPCMNT 6CTIDP|CTNMPCTOwPCTTL 6CpEPDATA@DATA@DATA@DATA@DATA PDATA PDATA P-DATA P*}DATA /_\1DATA /_1DATA /_1DATA /_y1DCHTDSamDySN 6dDye#DyeN6oDyeN6tDyeN6yDyeN6~DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 5EVNT 6EVNT6EVNT6$FWO_GATCGTyp!!6CHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~xLIMS!!6LNTD2LsrPaMCHNMODF6MODL3730NAVGNLNE`NOIS9P1AMDP1RLDP1WDDP2AMD P2BADD4P2RLD$PBASDD۰PBASDDlPCONDDHPCONDDPDMF\PDMFPPLOCD|PLOCDPSZE`PTYPPPXLBRGNmPtRMXV1RMdNHPRMdV1RMdXH`RPrNPBRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT  -)RUNT  Rate 67RunN""PRS/N%1SCAN@SMED SMLtSMPL""[SPACMSPACISPACMSVER4.0SVER SVER,,5Scal@Scan@TUBED3Tmpr<UserphARphCHgphDYbigphQLcphTRphTR)wThis is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN \(APXV2APrN 6APrV1APrX}}6ARTNASPFASPtASPtB1PtB1PtBCTSBufT PPCMNT 6CTIDP|CTNMPCTOwPCTTL 6CpEPDATA@DATA@DATA@DATA@DATA PDATA PDATA P-DATA P*}DATA /_\1DATA /_1DATA /_1DATA /_y1DCHTDSamDySN 6dDye#DyeN6oDyeN6tDyeN6yDyeN6~DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 5EVNT 6EVNT6EVNT6$FTab&\FVoc&KFWO_GATCFeat//&GTyp!!6CHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~xLIMS!!6LNTD2LsrPaMCHNMODF6MODL3730NAVGNLNE`NOIS9P1AMDP1RLDP1WDDP2AMD P2BADD4P2RLD$PBASDD۰PBASDDlPCONDDHPCONDDPDMF\PDMFPPLOCD|PLOCDPSZE`PTYPPPXLBRGNmPtRMXV1RMdNHPRMdV1RMdXH`RPrNPBRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT  -)RUNT  Rate 67RunN""PRS/N%1SCAN@SMED SMLtSMPL""[SPACMSPACISPACMSVER4.0SVER SVER,,5Scal@Scan@TUBED3Tmpr<UserphARphCHgphDYbigphQLcphTRphTRU[lWQXb^kOptpuD:\E+mHY`/&mayX9'-vyNJRA=57AMjCW`D/z\|DX&_lp2]m"]P"F77sI_6sKGol83p{ ]GxNu~)B*HUrG\W\lYT`M=4T*R7R8~f)NK[>Id`Po<_+MWQ}VX*oE}`Z&oRLV;);-ags5)9mW'>YP~jhM^,%bkb |{cokB}qHqHStXK#XTE-=[c}Sn2M5FiK&=RSbXW,62DmOYX/D?**s$[-p+D42F7f6QuA!9@1%ilJG82G [7P^E$62:gt]pu<~$%KP$cKOvx30S{s;%dF5A8o/4w;+(2o~*,p""#]eq }X'KD7I 9_[*?bCDkSOj!Q4M"}JMzR9JK[Yl)d762H#A#Q9U,Z:X=<;q4n%DSi^tpuze2#Xxiy?,1OPh~wA[h@tC]"#]}l~e,SXtmI^#lW4?/D:Q64Pot pN9IlVOY(xs/b@j/.=y`6@{u4f\TP/h;ZQkwqzFGP7T]V%R5;MmN2CgGz^nLoT.iPtj glX ,cW`e;E;qK|-Vz^b;0|>kij7!}WQ`QEJ9."G>}O!:i1At*ldAT/n@6TdO{7+VDSHM6FNmMOx^.EW<#G@njH[=K{_"#k6l2WeH?W~jHe(VZ#qKGkK1jVYLt)kWgw/|9vAMV^@Yo]p&qn~+lU,YTl_{1#p{XPTF}cyy3of)`|!-Vt2`\wmj773Ku\@\=5NDzU4oD7N**s$[-33.BkS7> YlGcRb[xy8J9tG>udrV=y`6@WBktK3c*6?FYT7~U+U*rR_"qaf/h7kZ5UPh_,zi8*rI.~s6Unm5X|r=F%+E p*=tu:wP?-1S-4L7=xU;C,6KP8)s5AVihwYc_`]:L]{RO7K57:=&CaF-B]%jyeY5+!hs|*>H~?p?}@8jaU(]y"/:Boz?xNu~)B*#qc3D44[_o5qS+SP7T]^q;,`h?&03S