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 " '  #                    d,,,III^^^{{{&&&///DDDNNNaaavkv      ***333>>>GGGGGGRGGGGGGRGRGRRGGGRRRRRGRRRGRRRRGGRRGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRGRRRRRRR;SSSKKKiii !!!+++444<=<EEEMMMVUU^]]eeemlltssz{z !!!"""####$$$%%%&&&&'''(((())))***++++,,,,----....////0000011112222333334444555556666677777788888999999999::::::::::;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: e8a630e676d54d71a0af637806f021bdSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-28_09-06_06723130Seq16716134697_134697mfagan96-Well"K1313_C8_Verbena_litoralis_matK-xFKB_3730_POP7_BDTv3.mobSeqPop7zHUC")29<;3%}wromjhe`XL;' "&!     *<@5  #>NL84?:'7bM *2* qOKd 8Znx{xst6ydYN6 K"-:4XslraOQfjN><DQ_mxvdK2 &298. 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"          !!!%%$        "$$"! #%#   #-1-" "&%                                                                                                                         !                                                                                                                        %OI@OAADA KB.bcpKB_3730_POP7_BDTv3.mobtermAACCGTTTTGGAGGGCATTTTTCACATTTAATTTTGTATTAGATATACTAATACCCCATCCAGTGCATGTGGAAATCTTGGTTCAAACTCTTCGCTATTGGGTAAAGATGCCTCTTCTTTGCATTTTTTACGATTCTTTCTCACGAGTATGTATTGGGATAGTGTTATTACTCCACGAAAAGTAGTCCTCTTTTTCAAGAGAACAAAGTTATCTTATCTAATAATTTCAGGAGTGGATGATCCTTTTCGCTTCACGAGCAATTCTTGACGACAACACTCGGAGACTCTGACATCATTCAGGAAAAGACGCTGGAAGTTTGGAAGCTAAGGTTTCGGCAACACGTGGTCAGGAGGAGCGCTTCGATAGGACCGAAAAACGTGCGGGTCAAGGACTCTCTGGGGTGAGAATGGGAGCTACTGCATTGTGGAACGACATTCGGGGGGTTCTCCCCGGGGGTCAATACCATTTCGGATATCCCTGATGGGGCGACAAGAGGGGCATGCACCTCCCGCAGGAACAGTCAGAAAGGATTCGAATAGATGCAGGAGGAGGCGGCCTTGGGCCAAATCCCCGGTCTAAACCGTTTTGGAGGGCATTTTTCACATTTAATTTTGTATTAGATATACTAATACCCCATCCAGTGCATGTGGAAATCTTGGTTCAAACTCTTCGCTATTGGGTAAAGATGCCTCTTCTTTGCATTTTTTACGATTCTTTCTCACGAGTATGTATTGGGATAGTGTTATTACTCCACGAAAAGTAGTCCTCTTTTTCAAGAGAACAAAGTTATCTTATCTAATAATTTCAGGAGTGGATGATCCTTTTCGCTTCACGAGCAATTCTTGACGACAACACTCGGAGACTCTGACATCATTCAGGAAAAGACGCTGGAAGTTTGGAAGCTAAGGTTTCGGCAACACGTGGTCAGGAGGAGCGCTTCGATAGGACCGAAAAACGTGCGGGTCAAGGACTCTCTGGGGTGAGAATGGGAGCTACTGCATTGTGGAACGACATTCGGGGGGTTCTCCCCGGGGGTCAATACCATTTCGGATATCCCTGATGGGGCGACAAGAGGGGCATGCACCTCCCGCAGGAACAGTCAGAAAGGATTCGAATAGATGCAGGAGGAGGCGGCCTTGGGCCAAATCCCCGGTCTA&?Qajt{ (1;KZgu &4>HUbly"2:IWdlw +7EQZepz  +8CN\eq}  ,7BN[dr+8?LV_lw'4<EO^lw  ' 0 @ M _ o |  - < N Z f n {  ' > L V h t   , ; K [ l   ) 5 @ J U g p ,<O]m*<K_j{)7LUcpy.>Tet"5AJ`n| $4<MZhv )9DR_iv~ '2EO^iw-8HQcm| ,5?M[gw"2?IQ]iv%0<JXbt"1=GS[co"0=MXcn| %.?FX`i|"+9BN\n}&?Qajt{ (1;KZgu &4>HUbly"2:IWdlw +7EQZepz  +8CN\eq}  ,7BN[dr+8?LV_lw'4<EO^lw  ' 0 @ M _ o |  - < N Z f n {  ' > L V h t   , ; K [ l   ) 5 @ J U g p ,<O]m*<K_j{)7LUcpy.>Tet"5AJ`n| $4<MZhv )9DR_iv~ '2EO^iw-8HQcm| ,5?M[gw"2?IQ]iv%0<JXbt"1=GS[co"0=MXcn| %.?FX`i|"+9BN\n}     /):" !2'3'3,;;&#"'+>+ ;';)1+ 33$.&3.!2!"!&+1'$6'2.(#   $"'".2..  " ++$                                                                                                                         /):" !2'3'3,;;&#"'+>+ ;';)1+ 33$.&3.!2!"!&+1'$6'2.(#   $"'".2..  " ++$                                                                                                                    C(<7*XU?.F 9HS=M"*C107VWS\(\3`Z& ]9:5 =!A_R@M0/=LP!a 4d9H&bLUU%:K8/'cEH$0"+6*NZ\,Y&'(a"PC3DMC Xdb9 V;&N'=B+P*9N^_'G:Ac":_(^G0AV/5 S 9M @;!S V1 G3L^A2P\XL Hb 6PHc > 'HX3P8-E^O Q6XaSM`KBHH$4ETI?-WY?Z`C-UJ'F95/]Q8>^#PTO2EOX1W*<X:'QF%@MJ!KK> 6EJ=1O])8c_591 10V S R<_E .63O-^W SF KV-5R\L+.:51/.I KW 0AA201PQ 'NJL_ B &26H K PA=Lc]? U K]7CL3\JB;U DWT0M J-,U=T-? 21S "5D9QO=KLR#3HVR7!@1P&]TE* 3 7W;>'Vc3DA]FW E#)6Z*= +AF4@6OH+%`\H0d^L7) Z5 F{YB@_|c<IsF,!$0t!n_FKE# OX %dL34j>C8~@Hd3C% fXyEwAx"!xQ#aQ"Q/P65I/0HTe)}6P[#g`*VT~Gk8b9@`|9ygSNrL&4f'>uiq$<?& &y7wiP + rG&o515]Z9 #+X[./$#Mr7(O  :r)7HHu8Y+Z0~D/ Rx#|w( z LzoG& +*iIevP5 ZI/V}hsi2<fGSfu ":kd9hBz9TIk}{i [:qO+_jgt.Q75eo=9;;i7?Uxh^_ a`#|x!P2h ^Vq\|\g]^<L`4bK@L8AHT9>g:G=Q79A1Z&/QE9V%E*)Rl?02z:aTf 4DdSPOGJ`A]N;RW((m:PS[P7'LC1j@k*/:-#*)PUQ<XG~Rd_0D||25% nS.~709I<H-iSIsxK2[Dx%Ba^0 D  bB(8% X|{`j/-9SdlR|!o@.9? Zt`A T6v0M2da}(B=h9/Io\Ik[ JDe8!BCGOeQr`H4<fyR.x9> 5N@oN#K94u+Kh?k`49Xw,mpUFNt|SpRcP*p~1:WRr9pp XY2sE5Aj8 AYZ,?O1j23.i2|KihD0n+ ,K#pdh@Ez23p#Rc!qSFzWi-qj[{WOcZxNk3~p/LB8iFn wkX `Hiatt`8y5)g{- 4 `F@M% @a@zqY0)H ,\)(TO7Tu5;3#pFni|%! QpT]["{, +? ,T.-2vl.@TSw@!@!I7y&$fiR\9%jG,yF@id[WWcM+O`4k2 {/d(^;.+\ {MB1:5$Qo4NoEd$WI83\% V>eRWl69xh^jifsOAS<xr|Ts7 (LMR'O&"I<6)3X6Us]BSXE5 4J`tkoFg- o (& @)%D r >|UlwP"cZFw$HK"b&[O|,+M'IV84Pu;~uU?o~rlwa qEyVU+3gFfdA@oi$,_UI { nRTTTTTCCAATAGACAACAGAGGACATACATTAGCATGTAACATATACTCTTAGGTTACAGACCGCGTATGTTGGTAAAATTGCACTTACTAATCGGTGCCCTGGCGAGACGACTAAGCGATGTCCCGAACAATAATCAATCTGGAGTATGCGGAACAATACCGTGATAATACTGGACGGGGCCGTATTATCCCCAATGAGATTTGGGAAATAGCCTCTGTTATTACCTTCTTCGAATGCTAGAACCCATTGGTTGTGCAGTCCTCATTAATTCGATTCTATGTTTCTAACGGAGTAGTCTACCTTACGTTCTTGCCCCATTGTCTCTGCAAGGGACGGCTTGTGTCGATTACCTGCATAACTTTGTTTTTTTGGGGTGAACTACTCGCGACCAGACTGTTTCAAGAGGATAATCGCTGGTAGCGTGTAGTGCCTTGATTCTTTTGGGGGGGGAACATGTCTACGACAAGGCATGCGTAGGATATTCTGATAGTCCATTCGGAAACTTCTAGTGGAACGTGAGTCAGGTACCGCTCGTGTTGATGGATGAAGATGAAAGGATTTGGGTTAAGTTTTCGCCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-28 11:10:29 -04:00AEPt@AEPt@APFN \)APXV2APrN 6APrV1APrX}}6ARTNASPFASPtASPtB1PtB1PtBCTSBufT PPCMNT 6CTIDP|CTNMPCTOwPCTTL 6CpEPDATA@DATA@DATA@DATA@DATA PDATA PDATA P-DATA P*}DATA /_\2DATA /_:DATA /_BDATA /_yJDCHTDSamDySN 6dDye#DyeN6oDyeN6tDyeN6yDyeN6~DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 5EVNT 6EVNT6EVNT6$FWO_GATCGTyp!!6CHCFGCEHCFGHCFGHCFG==InScInVtLANE<LAST~~LIMS!!6LNTD2LsrPaMCHNMODF6MODL3730NAVGNLNE`NOISZP1AMC+P1RLCP1WDCP2AMCP2BACCCP2RLC7PBASCCPBASCC؍PCONCCbPCONCCPDMF\PDMFqPLOCCPLOCCPSZE`PTYPPPXLBRGNmPtRMXV1RMdNHPRMdV1RMdXH`RPrNPBRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT  -)RUNT  Rate 67RunN""PRS/N%RSCAN@SMED SMLtSMPL##[SPACMSPACjSPACMSVER4.0SVER SVER,,5Scal@Scan@TUBEC8Tmpr<UserphARphCH؈phDYbigphQLcphTRphTRhThis is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN \)APXV2APrN 6APrV1APrX}}6ARTNASPFASPtASPtB1PtB1PtBCTSBufT PPCMNT 6CTIDP|CTNMPCTOwPCTTL 6CpEPDATA@DATA@DATA@DATA@DATA PDATA PDATA P-DATA P*}DATA /_\2DATA /_:DATA /_BDATA /_yJDCHTDSamDySN 6dDye#DyeN6oDyeN6tDyeN6yDyeN6~DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 5EVNT 6EVNT6EVNT6$FTabiFVocXFWO_GATCFeat..*GTyp!!6CHCFGCEHCFGHCFGHCFG==InScInVtLANE<LAST~~LIMS!!6LNTD2LsrPaMCHNMODF6MODL3730NAVGNLNE`NOISZP1AMC+P1RLCP1WDCP2AMCP2BACCCP2RLC7PBASCCPBASCC؍PCONCCbPCONCCPDMF\PDMFqPLOCCPLOCCPSZE`PTYPPPXLBRGNmPtRMXV1RMdNHPRMdV1RMdXH`RPrNPBRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT  -)RUNT  Rate 67RunN""PRS/N%RSCAN@SMED SMLtSMPL##[SPACMSPACjSPACMSVER4.0SVER SVER,,5Scal@Scan@TUBEC8Tmpr<UserphARphCH؈phDYbigphQLcphTRphTRKat)ecH<]O9Y}TU}xE_q-%Qvqfkv4)xJt;["*Kky#!#fn2lsa!ly^pd1v/gg61C`wWsV&&?-}U`aK|`Vc.O_l;5)F}oP5?z#`g5 xv*6.HNWZnPnG7+6'UH[2g[jWcgnW6Obe"`dbB-Y@iGu2tZG+F7u2]Hn~+luKnPv@+Idp"iK][UcydLC2fo;LY&0]g0b) e:=Us1HB+{fU2sA>i@8/(8\_pzN02%B4O7yr[q?Ib\f.KuDL0G_CPzFtSEH$*YYo%@rs{6`Vs=y I/ax]]?EZp9}:>Ju6v;(vJnPkGr#d#"0Koiw87=6"4#O|!fEW<#G@njH[=K}2?Yk".Om,4oFo;X.pHwT_v^GgPm#8gtKM@BS/h#*vC\R=Jg$-KqN^Fu*te9r=u4TYe0+Vsp@O3sT6#f 6Y?03t0mP@*26G !9W&a:Ocdmo-JmE;9xxsWD@TZmU)P? sz{fzVSA]!lZbCd]'n,J6v`ZdD\4A,h{|dpwM5rm;p=_A26O1HnYDx8_b_l4P3LFRt( g>::#Q[80_qmS8ULWcu\s|UN7\j7,'@_QWpcw!@_f,? 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