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!!!+++444<=<EEEMMMVUU^]]eeemlltssz{z !!!"""####$$$%%%&&&&'''(((())))***++++,,,,----....////0000011112222333334444555556666677777788888999999999::::::::::;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 4dfdc737cf5143aabb7b02d52904a4a6Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-28_09-06_06723130Seq16716134697_134697mfagan96-Well#K1313_C7_Hidalgoa_pentamera_matK-xFKB_3730_POP7_BDTv3.mobSeqPop7zYZao-i '35;Ox;x_ d p< o<gz458rB !77%bwM>S< Z 15XJ  TO 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H R ^ i y   ! , : K X g w   ' 4 @ R e s  ( 3 B T a r   $ 1 A N ^ g t *6CWfp~     % $   ( " 1+"  "  !         1+                                                 % $   ( " 1+"  "  !         1+                                            F2Q#I-QCG0, "=X$F^-$?$<WU*,])]EI [,(AB+OL6SQM*OF*ZBHGHZ L$ H#Y% ,7 .P"KYZ 0 '8G"` 9d Y: [T"(49OY-,>LP>A;<N] #K< F--C@*W/Y)V ZO F)]1` V8'7 1UJ9!&(,F]D6 G5bDL&S=;)Z!XY 1\WR)[RL>CX&% P<EM/XF0Z)Y<VW:b5J.?5>,CA];M!2P8`]6 1&.Sb!Z)MA?I< %&)[FGFK:>[3 @ 6><k?U e8jv `8V7/CQ ST@3W%c&_Gu|Mbk7AZ ^yXRmF=X`3(B<a`/:Kk6JjQn(T!]!f0 m5ZoXmEQuE`TNu %U Pd[!%H%q3`n#F_'  Pl'>X4w h HFs @YXW $b'*Q{mPiru|}zhB^8lovtoaI .dC_,8UzzO0F`Sb  >Yv@5"[c(D:|) s[ Q7[sP$@O>&,zc#62*WI16R9LeI. + u{ %F.X_p-pdIpEp#,?papp,GH%C$ U\ p)Yes{5(Aj?8#S?+MG cdj~uE02J<~]iRxeK>kd/la: n42*~(laIN7'Z  q kJZ R u',*f"S`H U \}vm-_S3W.5R %!9NL5}E ui(,1XNf7 6N}fcP+pT<A?W<T]F#Bqa9ayoVDvH.18~.;$ doPrMlA@R YP(LJjXj1T(3[lf;er nzQVQQ[zk+zmVQ!F$eZBPj&zym[AATTTTTTTTGTTTTGTTATATCGGAAACACACGCCTATGCAGGTTATATTACTAGTTCATGGGCTTCTGGAGTTAGTAACGTTGGACTCCGTACTTTCTACTTTTGAGATTGCCGTCACAATGCCCCGAAAGATATCAATCTATAGTGTTACTGAATCCTTAATGTAGAGCGGAAGATGCGGCCGGTTTCGCTTTCCGGGAATAGCGATTACCGCTTCAATACCCGTGGTGAATGTGCGTGCCACGTCCGTTGGCGACGGGGTGCGGAGGGAAGCCACGGTAAAAGACCTGGGCGGAGGGGGGGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-28 11:10:28 -04:00AEPt@AEPt@APFN \*APXV2APrN 6APrV1APrX}}6ARTNASPFASPtASPtB1PtB1PtBCTSBufT PPCMNT 6CTIDP|CTNMPCTOwPCTTL 6CpEPDATA@DATA@DATA@DATA@DATA PDATA PDATA P-DATA P*}DATA /_\3DATA /_KDATA /_cDATA /_y{DCHTDSamDySN 6dDye#DyeN6oDyeN6tDyeN6yDyeN6~DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 5EVNT 6EVNT6EVNT6$FWO_GATCGTyp!!6CHCFGCEHCFGHCFGHCFG==InScInVtLANE;LAST~~qLIMS!!6LNTD2LsrPaMCHNMODF6MODL3730NAVGNLNE`NOIS؛P1AM1bP1RL1bVP1WD1bP2AM1bP2BA11@P2RL1b|PBAS11PBAS11PCON11%PCON11PDMF\PDMFزPLOC1bݒPLOC1b0PSZE`PTYPPPXLBRGNmPtRMXV1RMdNHPRMdV1RMdXH`RPrNPBRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT  -)RUNT  Rate 67RunN""PRS/N%ؓSCAN@SMED SMLtSMPL$$[SPACMSPACثSPACMSVER4.0SVER SVER,,5Scal@Scan@TUBEC7Tmpr<UserphARphCHphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN \*APXV2APrN 6APrV1APrX}}6ARTNASPFASPtASPtB1PtB1PtBCTSBufT PPCMNT 6CTIDP|CTNMPCTOwPCTTL 6CpEPDATA@DATA@DATA@DATA@DATA PDATA PDATA P-DATA P*}DATA /_\3DATA /_KDATA /_cDATA /_y{DCHTDSamDySN 6dDye#DyeN6oDyeN6tDyeN6yDyeN6~DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 5EVNT 6EVNT6EVNT6$FTabTFVocCFWO_GATCFeat..GTyp!!6CHCFGCEHCFGHCFGHCFG==InScInVtLANE;LAST~~qLIMS!!6LNTD2LsrPaMCHNMODF6MODL3730NAVGNLNE`NOIS؛P1AM1bP1RL1bVP1WD1bP2AM1bP2BA11@P2RL1b|PBAS11PBAS11PCON11%PCON11PDMF\PDMFزPLOC1bݒPLOC1b0PSZE`PTYPPPXLBRGNmPtRMXV1RMdNHPRMdV1RMdXH`RPrNPBRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT  -)RUNT  Rate 67RunN""PRS/N%ؓSCAN@SMED SMLtSMPL$$[SPACMSPACثSPACMSVER4.0SVER SVER,,5Scal@Scan@TUBEC7Tmpr<UserphARphCHphDYbigphQLcphTRphTRom;PsqD57%6C}:*SM|aZA /GAjrS$I),_A#$O7{_O[T6S$gI{cTKcaza(RO[mOEW<#GnP Qc$@)kOie&>2 xX|L=6XO?Tve!@;wW14^M1}Gu9v_QjXa*`)l@.u!n`%Sz*1fCy:*0avFwn&K2fE[p!CODa)x+7E2]Lv3?%diR)6/lA^5ehJb@4}%P!SNmWT/OKOvx4G!?`U!9@mwyy>T3pb}045@u:r*nOrozc{G[ZI,|Q|T{yK{1P3;fTR[fRr;-YVJ|Q:{+ z9X=5>2jDTWy2>y^q3Th$Y thENWNv3[My J1\QWi.HhrjcngzE<=#d1a-cF~bvHN+MzL6L!<7J8_M4;-6`Zdg~P_F7a;A{%ipoWeV&jJDeVE+hj3EYWC.%obB`!3xrM?"Kml#@jC#zhJuR\l2=,A 0wz`&1ZG|JW/e2FVc2(_CSR{[}7"=Iw<1C{_@6tKR"\pI2r>6Z~=}F)h>xgY5"KBv'o>s1HT&}J39OQJMeP> A(_GZE?RVuU,ZBE=+^k42g|5[S*.JXs2M*;N/VMR{7gmUo:e.s$;_CP)DzEC>3f9bzL4z`=~KmZlqNPNsd}FlnqNUot6bN=bW}@Kp]R[gF{\)lOY@ZWeoak"T'o0_s2#O|!f|bEdG~_ {JH:,bQ2a5|OjAZeXVm? >S]T'_C:Or4^7~s?K90lV)XHT1yqX6\qwH=qd)B*#k)lO[vHd55-z]T1jzLx2&,x}ka03(_yb;E26?^V$Yeaejp~K]yDWUhcH[X]Lu:ZqLNDI 7&o.1r9v[?|$}~?V'i~?RVuU,H/`WajZUTw|(F}K7T]T%E+D0.98xOrz6 ?63_1bTio0SbXW,VE+,[Ol% eIe]kij7!}WQ`QEJ9."G>}O!:i1At*ldAT/n@6TdO{7+VDSHM6FNmMOx^.9,(B~b4O{[=Ku%9tWa-cZ,\|b=-j%`3EFilpAEPt@AEPt@APFN \*APXV2APrN 6APrV1APrX}}6ARTNASPFASPtASPtAUDT gB1PtB1PtBCTSBufT PPCMNT 6CTIDP|CTNMPCTOwPCTTL 6CpEPDATA@DATA@DATA@DATA@DATA PDATA PDATA P-DATA P*}DATA /_\3DATA /_KDATA /_cDATA /_y{DCHTDSamDySN 6dDye#DyeN6oDyeN6tDyeN6yDyeN6~DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 5EVNT 6EVNT6EVNT6$FTabTFVocCFWO_GATCFeat..GTyp!!6CHCFGCEHCFGHCFGHCFG==InScInVtLANE;LAST~~qLIMS!!6LNTD2LsrPaMCHNMODF6MODL3730NAVGNLNE`NOIS؛P1AM1bP1RL1bVP1WD1bP2AM1bP2BA11@P2RL1b|PBAS11PBAS11PCON11%PCON11PDMF\PDMFزPLOC1bݒPLOC1b0PSZE`PTYPPPXLBRGNmPtRMXV1RMdNHPRMdV1RMdXH`RPrNPBRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT  -)RUNT  Rate 67RunN""PRS/N%ؓSCAN@SMED SMLtSMPL$$[SPACMSPACثSPACMSVER4.0SVER SVER,,5Scal@Scan@TUBEC7Tmpr<UserphARphCHphDYbigphQLcphTRphTR