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'  /  $'               #J%d,,,III^^^{{{&&&///DDDNNNaaavkv      ***333>>>GGGGGGRGGGGGGRGRGRRGGGRRRRRGRRRGRRRRGGRRGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRGRRRRRRR;SSSKKKiii !!!+++444<=<EEEMMMVUU^]]eeemlltssz{z !!!"""####$$$%%%&&&&'''(((())))***++++,,,,----....////0000011112222333334444555556666677777788888999999999::::::::::;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 334f769eb72d4f0ba6d459b472e69a33Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-28_09-06_06723130Seq16716134697_134697mfagan96-Well(K1313_C1_Peperomia_pseudoalpinia_matK-xFKB_3730_POP7_BDTv3.mobSeqPop7z(*+(! 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TTATTTTTAAATAGATATAATACTTACATTTGTATGTGACGATATTCTCATAGTCTAGAACATTCCTCCAGGTATGGATGGACGACTTCAGCGGCACTCCAAGCCAATAGGGGGCGTCGGCGCACCTAAGGCTACTCCCTATACGAGTGTATGGCCAGTTGTACAGCATGAATACATCGGAGGTTCGGGGGGTCGACCCGGTAGGTATAAAGAGACGCTAGTTAGTGCCATATAAGCAACGTTGTGGCAAGAATCCTGTCGCACCTGCCGATTATCGCGTGGCACACGGATATCAGTCTATCGACGAGTTTACGCTTAGGGAGAACAAGTCAGGGTATTTGTAATTTCAACTTCTACGGTTAGGGCCTCTCCGTTTTTCTGTTTTTTTTTCACTTACCTGAGGAATCCCACTTCTTGTGACTTAATGGTCATGCTCATCTTCCGTGAATGGTAGTTTCCTGGACTTGCCACATAAGCCCGATACTTGTCTTCGGAACGTACCTAGTTCGTATCACAGTTGTCTCCCTGAATGGATTCGGAGGGTCGCAAATGGATAATCGTATACCATGGGAATTATTGTCTGAACTCTAATBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-28 11:09:33 -04:00AEPt@AEPt@APFN \/APXV2APrN 6APrV1APrX}}6ARTNASPFASPtASPtB1PtB1PtBCTSBufT PPCMNT 6CTIDP|CTNMPCTOwPCTTL 6CpEPDATA@DATA@DATA@DATA@DATA PDATA PDATA P-DATA P*}DATA /x^\8DATA /x^(DATA /x^DATA /x^yDCHTDSamDySN 6dDye#DyeN6oDyeN6tDyeN6yDyeN6~DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 5EVNT 6EVNT6EVNT6$FWO_GATCGTyp!!6CHCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~#LIMS!!6LNTD2LsrPaMCHNMODF6MODL3730NAVGNLNE`NOISP1AMPSP1RLPP1WDPP2AMP3P2BAPPP2RLPPBASPPڃPBASPP3PCONPPcPCONPPPDMF\PDMFPLOCPsPLOCPPSZE`PTYPPPXLBRGNmPtRMXV1RMdNHPRMdV1RMdXH`RPrNPBRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT  -)RUNT  Rate 67RunN""PRS/N%SCAN@SMED SMLtSMPL))[SPACMSPACSPACMSVER4.0SVER SVER,,5Scal@Scan@TUBEC1Tmpr<UserphARphCH.phDYbigphQLcphTRphTR&bThis is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN \/APXV2APrN 6APrV1APrX}}6ARTNASPFASPtASPtB1PtB1PtBCTSBufT PPCMNT 6CTIDP|CTNMPCTOwPCTTL 6CpEPDATA@DATA@DATA@DATA@DATA PDATA PDATA P-DATA P*}DATA /x^\8DATA /x^(DATA /x^DATA /x^yDCHTDSamDySN 6dDye#DyeN6oDyeN6tDyeN6yDyeN6~DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 5EVNT 6EVNT6EVNT6$FTabFVocFWO_GATCFeat..GTyp!!6CHCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~#LIMS!!6LNTD2LsrPaMCHNMODF6MODL3730NAVGNLNE`NOISP1AMPSP1RLPP1WDPP2AMP3P2BAPPP2RLPPBASPPڃPBASPP3PCONPPcPCONPPPDMF\PDMFPLOCPsPLOCPPSZE`PTYPPPXLBRGNmPtRMXV1RMdNHPRMdV1RMdXH`RPrNPBRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT  -)RUNT  Rate 67RunN""PRS/N%SCAN@SMED SMLtSMPL))[SPACMSPACSPACMSVER4.0SVER SVER,,5Scal@Scan@TUBEC1Tmpr<UserphARphCH.phDYbigphQLcphTRphTRPy]]~I8[e0MSayQckycji[&`&h2hA:DCDFIt~FRi^aL7(p~XVG ;W/0'o ocDGRFT16Y-e+&5%`7lZ!O)>C9iu2Zv&1x5roz;P]pDuYLDK2NB&_SF`|z&mWAeAT%{AB02oGhnW"KuQtb'J!>yHPx%bg*p/YgnCCHY<6(mu y-WLa)&:\.~x|MI>"J'8O[,_a2'Kdb"U"=C!(3by{,ty\bH?6g%3tk?$[WturW>4GPQEdrB-8'i2{ysPl`A?^NHEDX+9m#k:X=<;`O|g+4b|U:h t$~!(mO_YT[3T+/qIlE]&l8EE.Om,\eyDWYI!8U-o9D4lxXW[uTs|$@^]?\Hif3qE`HoG83:8\_pf|r,!b&l]uD=O?HS|G;~ip@2&Rsn!)&*yOD5`@CAIx*l*wa:t"@>oW~!Eb4;,}|O[nYI.xv^$beP *"udrVLgJRv)1mfc4hAC?-4$&LkfG;7oF`|zvn6&pg;gjmMUwch[}k@_ajFoW~&WZ$>tSEH[!]fKOvx4G!?`T/Hf.X7GtX6|k.j,PCH-p}*4d#R:c@ZIIC"&;UXU8x[K"4#O2C.98xOrz6 ?63_baI23|7hhSc%bVm? 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