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!!!+++444<=<EEEMMMVUU^]]eeemlltssz{z !!!"""####$$$%%%&&&&'''(((())))***++++,,,,----....////0000011112222333334444555556666677777788888999999999::::::::::;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 9f39420b8e9e4bf0a83054a2a102460aSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-28_09-06_06723130Seq16716134697_134697mfagan96-Well K1313_A9_Pilea_pubescens_matK-xFKB_3730_POP7_BDTv3.mobSeqPop7z +?Teqya6  sX>'5Rs-U*FUVH10Ic{,f_-T%rM#yrjd`\XTOKIJKNOOMIE@;5.%  &2=GPW^chlnnlga[WVX_k}|piinu|~yrkebbdfhe\N<+'-/*"    '2<BB<4/2A[{~vrneU?( &5BGE<.)361'  ?cC qN9S*(5:3# '., #++#  ->HG<* (Aa  tM- +>O[aa\UNJHLWi,J""'/zF) &+,(! 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R0- X cS#%oITvVpho[ZMPJ]bV]Fn@xO+?UY,L(\t$+)="W?D%.R*7@1d;5I &*62L4a65N$~/F=9133,2*2$&G8`11W3>q l"'0F"24!3-HdpppIpV3mH:OwUzUi=p[d1?klpUpEpMgGd~W}Ne_W87ppplRB*p,E8p?HYCuNpr<n[^pQ,"pdw}uJjdp1Opylid,,~\,$ 3aA17,>Ap,8k[!p,\(d,,FT%)6K8 PdLdddd8%$ Od q#J3dy5{d[p0|E|yHVIK? { <* PAv:v$e\  iqg7rLwg;4Lb?T`tes\eice[~IagJe9G9h0]2y.)Pp s^/O1}P} Cl\~sG.fp VZ77BZv&zN]_*r8iA$!m~* {S<9dz `OW,@m (+" 0r p6fPG1')]9$ BuZ)'Hc%-@sj@N":% K^:t{ps9^% s:: 8[E%S)NS[% M QS}Bd"v-$Hn0nM|?n=.DM%+f|2dCbc{j9=usAATTTTCCATTGATACTAACAACATACATGAATATGTAACATATGCACTTAGGGGCTAGGATAGACATGGAGGTAAAAGTGAACTCTGGACACACTGCCATGGTCGATACGACTCCGGCATGTACCTAAGAGATACAAAACCTCGCGACTAGGCGTAATCGTAGAGGCCGAATACTTCGGGGGGAGGGACCCCTACCCGGGCGGGGGGGTATAGGAGGGGGGGCGTCGTGCGTGCATGCGCTGATGGGTTAGGACAGGATCGGTCGTACAGGGGCGGCAGAGAAATCGTGATAGGAAGTCGCAGTCAGCGCGTGTGGGGGGAGCGAGTTGAATATTAAGGGATAAGTGGTGCTTTAAAGGGACCAAGCGCGGGATAGGGCGGGAGGGACGTTTCTGGGGCCGCGCATGGAGGGTCGTGGAAATTAGTTTTATTTGGGAGACAATTCCACCTGAAGGGAATCATTTTCCGGTGGAAAAAGGAGGTTTCCCGTCCACTGTGATAGCGGATTAGTCGTCCGTCGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-28 11:10:50 -04:00AEPt@AEPt@APFN \'APXV2APrN 6APrV1APrX}}6ARTNASPFASPtASPtB1PtB1PtBCTS/BufT PPCMNT 6CTIDP|CTNMPCTOwPCTTL 6CpEPDATA@DATA@DATA@DATA@DATA PDATA PDATA P-DATA P*}DATA /r^\0DATA /r^DATA /r^DATA /r^xDCHTDSamDySN 6dDye#DyeN6oDyeN6tDyeN6yDyeN6~DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 5EVNT 6EVNT6EVNT6$FWO_GATCGTyp!!6CHCFGCEHCFGHCFGHCFG==InScInVtLANEOLAST~~LIMS!!6LNTD2LsrPaMCHNMODF6MODL3730NAVGNLNE`NOISP1AM UP1RL CP1WD gP2AM P2BA  P2RL yPBAS  PBAS  PCON  :PCON  1PDMF\PDMFPLOC PLOC  PSZE`PTYPPPXLBRGNmPtRMXV1RMdNHPRMdV1RMdXH`RPrNPBRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT  -)RUNT  Rate 67RunN""PRS/N%SCAN@SMED SMLtSMPL!![SPACMSPACSPACMSVER4.0SVER $SVER,,5Scal@Scan@TUBEA9Tmpr<UserphARphCHphDYbigphQLcphTRphTR*ׁThis is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN \'APXV2APrN 6APrV1APrX}}6ARTNASPFASPtASPtB1PtB1PtBCTS/BufT PPCMNT 6CTIDP|CTNMPCTOwPCTTL 6CpEPDATA@DATA@DATA@DATA@DATA PDATA PDATA P-DATA P*}DATA /r^\0DATA /r^DATA /r^DATA /r^xDCHTDSamDySN 6dDye#DyeN6oDyeN6tDyeN6yDyeN6~DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 5EVNT 6EVNT6EVNT6$FTabFVocxFWO_GATCFeat..JGTyp!!6CHCFGCEHCFGHCFGHCFG==InScInVtLANEOLAST~~LIMS!!6LNTD2LsrPaMCHNMODF6MODL3730NAVGNLNE`NOISP1AM UP1RL CP1WD gP2AM P2BA  P2RL yPBAS  PBAS  PCON  :PCON  1PDMF\PDMFPLOC PLOC  PSZE`PTYPPPXLBRGNmPtRMXV1RMdNHPRMdV1RMdXH`RPrNPBRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT  -)RUNT  Rate 67RunN""PRS/N%SCAN@SMED SMLtSMPL!![SPACMSPACSPACMSVER4.0SVER $SVER,,5Scal@Scan@TUBEA9Tmpr<UserphARphCHphDYbigphQLcphTRphTRA08^#5EN^D\we*S#ibu@Qe7EG>x'H1PSpj:6R}ag5d9w];H:'~%b9DBjh'&~o[k7MLr92U*a!ZCCd+4ko)Yr13.iOW/D-]$#{N.g\7dHm,_~/S9D>)ecH<]%9t}TU}xE_q-%Qvqs@dUa_e^W^4c Y \,yvMU:6%4sI}]66{6sA bBTd.u?zAw3+fc`]i>\7F4r&\.36b=Jhyp%~:!VO_5M%_|_n?NhN{C]J=E]V&=o>ZEZ,*`y{XW,@|DmOYXm`s6)\R5y=Y`g%gcmea_';_/e"^=0UA!%EeKOWQ`QV-@[3>>B)u:[owQC(vJnPE*F;2_/gYFi$t_SF`|zvn=Y`1&!AuJUo6;^F6@_=!)}W}oo-o;X^`>EY$y!TW3nG6`VX+*fl{iG>A.8X:B?r@i$!^h{we**,ACT;fQGE-`x&i)6JWT|*0_fs$X=<;#(":?S`,j.uFTcM#yKA#2rBA++m}Il|NUq|1c$|(w7?*,CqB (_e:RJ4ir$G(~}d M]f)C^DZ=<7:cmj^k-T0M5[S*.JpxcSX`"lef5U_9}|Ate1}1"/bW>y&bC]LVn!<|'k0|3.B+0Oj!Q4X+*flt78{uCS]n?KGxDIBG(XX7&ZoMQR,9hLAEPt@AEPt@APFN \'APXV2APrN 6APrV1APrX}}6ARTNASPFASPtASPtAUDTB1PtB1PtBCTS/BufT PPCMNT 6CTIDP|CTNMPCTOwPCTTL 6CpEPDATA@DATA@DATA@DATA@DATA PDATA PDATA P-DATA P*}DATA /r^\0DATA /r^DATA /r^DATA /r^xDCHTDSamDySN 6dDye#DyeN6oDyeN6tDyeN6yDyeN6~DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 5EVNT 6EVNT6EVNT6$FTabFVocxFWO_GATCFeat..JGTyp!!6CHCFGCEHCFGHCFGHCFG==InScInVtLANEOLAST~~LIMS!!6LNTD2LsrPaMCHNMODF6MODL3730NAVGNLNE`NOISP1AM UP1RL CP1WD gP2AM P2BA  P2RL yPBAS  PBAS  PCON  :PCON  1PDMF\PDMFPLOC PLOC  PSZE`PTYPPPXLBRGNmPtRMXV1RMdNHPRMdV1RMdXH`RPrNPBRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT  -)RUNT  Rate 67RunN""PRS/N%SCAN@SMED SMLtSMPL!![SPACMSPACSPACMSVER4.0SVER $SVER,,5Scal@Scan@TUBEA9Tmpr<UserphARphCHphDYbigphQLcphTRphTR