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!!!+++444<=<EEEMMMVUU^]]eeemlltssz{z !!!"""####$$$%%%&&&&'''(((())))***++++,,,,----....////0000011112222333334444555556666677777788888999999999::::::::::;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: eb27dcd7c3a94a009672f84d05a75324Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-28_09-06_06723130Seq16716134697_134697mfagan96-WellK1313_A7_Bidens__alba_matK-xFKB_3730_POP7_BDTv3.mobSeqPop7z$@\x3LZcq4EC3-Pvu^?BtZNB)y5sL* %Hq )S I~ugnR1[ @OUW]jxuV%hQ8D:mS9.;9+ 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KH!O1O"E@K1:F)#>* X] 639P 68]QX( E+ 1D A-$$BA8)?1,CS KXR /R H8:L'(TYG:T);@/+?]L +=dQA3Y#OF .&!:T]669X[_W'/LIG:<FcLK3Wb OH!DB-Q& %_T**5]`)+! .\G=7; MR94JG\< W^_;5'VCO&J=CIL4[ 7T#'Q1#]d+())D^C!:]%T8ZY^2c.?#&\[&/:)d* 2*( @RV)V+7 +V[2MD/2OI=D\$LMFLN-a92M' #HbM6^#2&()+8&O>`M$F"_ I 22<^H2[!FR#3+6V2'KEX.RL'2>%2B_U2#G T2K2^-2; XS7!9222dJ124X4P(047%GN&L-,WJ211%><E5>8,'OY 09%M"Y 6ZY CLY2 MUSK>S!+PF42C,N @;2 19MJ<3(O' >3=`A#L ,FLG 2 2D,P :2 -\7d22R!22Z2-Q"c12C1\>ZBB!XI"DA2J";V4U2Rf/1 bm|*  t,"btfZau\3l* 1LZZBn`a&x;xy8"_s U44@5YB\!k>xv-$+]z`7Io^VXp8"+0/n.\B:kE[:k:AzA{qyuU 3++LnXLSVm}$nwmY>v7KOx)^{NA:sI{(2X/A,{BU6~.rf3 'p5l\cb69P1#/\G}s;9Jz['wBLL,Re{G\b~ [W!,%[q3 $ gZ>Aq;\7696A8; 0?K`y>9tgCR?|g ",;ny}p[6N(L3 @;EfM Z(=Y2QN oi_bgpF!Z\ +zEq.K$&j4#  p}dr3  Fmh7P}3  #Xx*_ 0"@hRZq1]F7"wmv`OPg_NFPCDC8MTCQ]'P2ICa>{~A5.4"9S 2 23, ";`x( >#*;K&' $!:% )G+A9,/48!THLT  + < $ 1/' '#3#Q9)8)"R?#m%.# 0X4C)!Vq4)U5 T/+=2&G$ 7JR,  %e#<J +1HN<#'.  4`@W4;(UB#3%K.,B> lH8?#-C$&W/4M#M 7 Q42;8C'''#(/''&'>)0091(_ :\  K 200@ rNF/@;Y   CXwq@   8  CCC #:TCC:  :CC`CCCY :CCCCCbb&&bRbbbb&&RRRRf9RRjbLbb&1RL&&&&R#nbbb^888,W^88WWWMW^^Y7^^^^^aWW^^^7@88WWWz'^^Y^____dllH\\\:ve?dllv\\,\\d__ }{hldddddl\dyyyy@yyyWOyLWMBcWWvWWy]Q7V]]]{w]+wM+?JE~oDp%Xpmpe 9Olb*l&  ~EZP 3p @[BI fdpS^lpZ!ajIK#H'e9v*e:f5S7P'UN:82pd@C8NpdZppE,o@d$dbTp,8J>fZ1^y@c0B>]F,U,@7p9b([8+~Sti^d;pcppp[aKyppxc%pdpp8,uCpp %$14D0a? k a,<p6Sy_5R1CixM=FX3SJNqwMG2y[mAD@L3\TN 9I]Ua^|yVag^V}/8ypnQ1byJ7ZqL[vfxv%n=.gsTC3T`&ujLh`l{L1JIO`PX96HV8^#&bM0q2`/{ms>}bE-Q" E6[H V[lp$PuL(<V9t9Liw}[b-TmSG8HU{vW{N{^.f oW4? ]q ^XmI[%LH@nh)O%)AMUwoE{cPQRuLNrpw#P1LK5/c}Y C 9?fo/[$>S>{ q/NT%ER|Og<:lm&\:C>$FV4% &%vmvCNs!q/ zU,:22ATTTTTTCTATTAGACAACTCGGGACACAAATTAGGATGTAGGATATGTTCCTGGTTCACATACTAAAGAGGATGGTAGCATTGGACTCGAAGAAAGATGAATTACTCGCTACGCATTATGCATTTACATGGGCCTAATAAATCTTCAATTCCGTCAGTCACCATATTGGACCACTACAGGGGCAGCTTCGTGCGAGCCCTCATAAAAAACGTTGATTTAGGATTGGCTAATCAGGCGTCGGGACGCGAACGATGGCCCCTTATAACTGGCCATCCAACAAGAAATTACGGGAAGAAGAAAACGGGGGAGAAGCTTATCCAACTTCGTGCGGAGAGGTTGTAGAGGCGCGGGCGGGCTAGAGCGTAGCCCGGAGGTGTCAAAGGGCCCGCGGCGCCCTGGTACGGACAGATGAACGGCTACTCGGGTACGAGTAGGGATTATCCAGTTGCGTAGGGAATCGCGCGGCGAATCGATGAAGCACGTAAAGGGGAGTAGGTACCCCACCCAAACTAGGTCCAGGTAGCATAAAAGCATCCGTTGACAGAGGCAGTCATAAGGTATCAGATGGGCGAATAGGCATCAGTGTAGAGACGGTCAAGATATTGACCGTGTGCGAGCGTCTCATAAACACGCTGGCCACTGAGTGTACATAAAACCTAACTTGTAGGGAGGTTATTTATGGGGATATGTCTCGTGAAAACTGTCGCTGGGCATAAATGAGACCTGTCCGCGABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-28 11:10:32 -04:00AEPt@AEPt@APFN \$APXV2APrN 6APrV1APrX}}6ARTNASPFASPtASPtB1PtB1PtBCTS3BufT PPCMNT 6CTIDP|CTNMPCTOwPCTTL 6CpEPDATA@DATA@DATA@DATA@DATA PDATA PDATA P-DATA P*}DATA /y^\-DATA /y^DATA /y^DATA /y^yDCHTDSamDySN 6dDye#DyeN6oDyeN6tDyeN6yDyeN6~DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 5EVNT 6EVNT6EVNT6$FWO_GATCGTyp!!6CHCFGCEHCFGHCFGHCFG==InScInVtLANE?LAST~~LIMS!!6LNTD2LsrPaMCHNMODF6MODL3730NAVGNLNE`NOISP1AMP1RL P1WDP2AMP2BA P2RLTPBASPBAS0PCONBPCONdPDMF\ PDMFPLOCPLOCPSZE`PTYPPPXLBRGNmPtRMXV1RMdNHPRMdV1RMdXH`RPrNPBRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT  -)RUNT  Rate 67RunN""PRS/N%SCAN@SMED SMLtSMPL[SPACMSPAC SPACMSVER4.0SVER (SVER,,5Scal@Scan@TUBEA7Tmpr<UserphARphCH+phDYbigphQLcphTRphTR.^This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN \$APXV2APrN 6APrV1APrX}}6ARTNASPFASPtASPtB1PtB1PtBCTS3BufT PPCMNT 6CTIDP|CTNMPCTOwPCTTL 6CpEPDATA@DATA@DATA@DATA@DATA PDATA PDATA P-DATA P*}DATA /y^\-DATA /y^DATA /y^DATA /y^yDCHTDSamDySN 6dDye#DyeN6oDyeN6tDyeN6yDyeN6~DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 5EVNT 6EVNT6EVNT6$FTabFVoc}FWO_GATCFeat//NGTyp!!6CHCFGCEHCFGHCFGHCFG==InScInVtLANE?LAST~~LIMS!!6LNTD2LsrPaMCHNMODF6MODL3730NAVGNLNE`NOISP1AMP1RL P1WDP2AMP2BA P2RLTPBASPBAS0PCONBPCONdPDMF\ PDMFPLOCPLOCPSZE`PTYPPPXLBRGNmPtRMXV1RMdNHPRMdV1RMdXH`RPrNPBRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT  -)RUNT  Rate 67RunN""PRS/N%SCAN@SMED SMLtSMPL[SPACMSPAC SPACMSVER4.0SVER (SVER,,5Scal@Scan@TUBEA7Tmpr<UserphARphCH+phDYbigphQLcphTRphTR.iTr8p|_1{63_`EHsMT@OmAmDe8HR/D2GcA13woF$1PI?p":*Xq)apMf4S><3$G,lwd-l#frL?*Y\FX`@kjJ}B~xGH$i*8j|]]u.ibuILW&-3DLVzMysP!b`}9(2&Sy'"jet?[)1-lHLl}gF$eD:91VuT(A\&C9":?ZEq1hu$#k:X=<;3On&+VN;172e,qz7|!Y<,h`Fzmj7Ul*8mlqRZ9Kn#_[6! 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