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!!!"""####$$$%%%&&&&'''(((())))***++++,,,,----....////0000011112222333334444555556666677777788888999999999::::::::::;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 4d774d9ee46541c5b847bb65054373ccSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-28_09-06_06723130Seq16716134697_134697mfagan96-Well'K1313_A10_Catopsis_sessiliflora_matK-xFKB_3730_POP7_BDTv3.mobSeqPop7zWa<!$<[y|z{qe\SI:"ue[O?, 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"*-*#                               ""   ""                                                            (-/-("     ')%  &-/-&    "#                                                                                                                                                                                                                                                                                                                     -o@oA'AA9XA!-KB.bcpKB_3730_POP7_BDTv3.mobtermTGAAGCTTTTAAGAGGCAAATTATCACATTTAATTATGTGTCAGATATACTAATACCCTATCCTATCCATTTGGAAATCTTGGTTCAAATCCTTCAATGTCGGATCCAGATGTTCCATCTTTGCTTTATTGCGATTCCTTCTCCACGATATCAGATTGGATAGTCTCATTACCCGAAGAAGGGTTTACTTTTTTCAAAAAAATAAAAGAATATTCGGTTCCCATGTATTTCTTATGTAGCTAATGGAAGTGGGAGCGTTTGCTCCGGAACGATCTGCGGATTGCGATGAGGCTAAAGGAACCTTCAAGGGCGAGTACAGGCTTTTGAAGCTTTTAAGAGGCAAATTATCACATTTAATTATGTGTCAGATATACTAATACCCTATCCTATCCATTTGGAAATCTTGGTTCAAATCCTTCAATGTCGGATCCAGATGTTCCATCTTTGCTTTATTGCGATTCCTTCTCCACGATATCAGATTGGATAGTCTCATTACCCGAAGAAGGGTTTACTTTTTTCAAAAAAATAAAAGAATATTCGGTTCCCATGTATTTCTTATGTAGCTAATGGAAGTGGGAGCGTTTGCTCCGGAACGATCTGCGGATTGCGATGAGGCTAAAGGAACCTTCAAGGGCGAGTACAGGCTTT%07P]jt}+3>N]iu#+8CM[hq (6CQ]jt} &0<JS]gu}%2<GP]ht &3AHUblx#0@M[gq~)9DO[jv   " 2 = G P Y f s }   $ 0 9 C S [ d p ~   & 4 ? M [ h q ~    ( 4 ? H R ^ j s ~   - < J Y g u  #,8IT^ft%07P]jt}+3>N]iu#+8CM[hq (6CQ]jt} &0<JS]gu}%2<GP]ht &3AHUblx#0@M[gq~)9DO[jv   " 2 = G P Y f s }   $ 0 9 C S [ d p ~   & 4 ? M [ h q ~    ( 4 ? H R ^ j s ~   - < J Y g u  #,8IT^ft        0#  &0& *+8!)/,883;+8, ;+, ;>;.(6 2($6$$;&'&$  "      "$                                                      0#  &0& *+8!)/,883;+8, ;+, ;>;.(6 2($6$$;&'&$  "      "$                                              )LE &N8$7R>I\*&N) U>1'^^53X$ W@M&`=N$ 4'HA2(F=41 DM/ B?-c!TY5X <M3EVNU&.[ %<; BF#,J0&X/1OIY+,^LI)G I 5G?U9!L[1%$M<c@16 / '2G3\K>V/T&8dJ'FU"a: "FB"6B( W<"%7-"B 6a;;O;#G2V;P.R2;;;)):^2)2*K;)2W7_A!7852/ 0N1U8C2,CGIEYCQ82, 8KM63,40b._ ]&=F2,8.2M?  Sa@pvK~u;dh^WqF1c<*'v;AC0}n%D,E.Pqx/iVJQrtEr(2__i14m:!.whJDMV0}*'FLHoSd.;@ ,\>7I8(L`$HQ#66[;*mmexcK]LT"rPUU{$WU? ( "{ 7GzqxB H0 > AZ 0NP[uRTddZ\UO{e]VAhUUTMo@*?HO89dF$7@cgp]vI)@\0^TMVAJE_vaQSEGaRk%[CZUeEu > l`y25H6-i2H]p+R{xi;VdJGZ_,.,Rpt_A,dsp&A!R+B p)gb7-4qMh G[J XYrVL 5gxa,s)U_p;2,5d dVdd ,b,^dKf'.f8`pdy)%b ]u 1* $)6}a[x^,CDU @?TX/=k]_DyWcY%}q}(n:@G>X4 yS, `Rdwb ElapKxih7R#+Qw si3A GDlK|GZsbw4Bk77$# SmTfYx $;^UEiF;bVWATTTTTCCCATTAGACACGTAGTAACAGACATCAATATGTGACCTATAGTCTTACATACTATTCCAAACACGTTGGTATCATTGAACGTATGCAACTGTGGCATAAAGTTATGAGGCATCGGTAACCTAGAGAGAAGGCAACTACAAGATAATAGAGTAGAGAGGCACAACTGGCGGAGGTCCGACCGGCCCCGAGGGGGGGAGCCGAGCCTAGGCTGGTGTTAAGGAGCTGGTCAGTTGACGGCCGGTATTTTTTTGCGTGGTGCTGGACGCGCTGTTTAGAAAGAAGATGGTCGAAGCATGGTCTTACGCGAAGAGATGAAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-28 11:10:51 -04:00AEPt@AEPt@APFN \.APXV2APrN 6APrV1APrX}}6ARTNASPFASPtASPtB1PtB1PtBCTSBufT PPCMNT 6CTIDP|CTNMPCTOwPCTTL 6CpEPDATA@DATA@DATA@DATA@DATA PDATA PDATA P-DATA P*}DATA /q^\7DATA /q^DATA /q^DATA /q^xDCHTDSamDySN 6dDye#DyeN6oDyeN6tDyeN6yDyeN6~DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 5EVNT 6EVNT6EVNT6$FWO_GATCGTyp!!6CHCFGCEHCFGHCFGHCFG==InScInVtLANEPLAST~~LIMS!!6LNTD2LsrPaMCHNMODF6MODL3730NAVGNLNE`NOISP1AMDP1RLDP1WDD*P2AMD:P2BADDP2RLDPBASDD>PBASDDPCONDDPCONDDߒPDMF\PDMFPLOCD PLOCDڂPSZE`PTYPPPXLBRGNmPtRMXV1RMdNHPRMdV1RMdXH`RPrNPBRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT  -)RUNT  Rate 67RunN""PRS/N%׿SCAN@SMED SMLtSMPL(([SPACMSPACSPACMSVER4.0SVER SVER,,5Scal@Scan@TUBEA10Tmpr<UserphARphCHphDYbigphQLcphTRphTR)jThis is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN \.APXV2APrN 6APrV1APrX}}6ARTNASPFASPtASPtB1PtB1PtBCTSBufT PPCMNT 6CTIDP|CTNMPCTOwPCTTL 6CpEPDATA@DATA@DATA@DATA@DATA PDATA PDATA P-DATA P*}DATA /q^\7DATA /q^DATA /q^DATA /q^xDCHTDSamDySN 6dDye#DyeN6oDyeN6tDyeN6yDyeN6~DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 5EVNT 6EVNT6EVNT6$FTabFVocFWO_GATCFeat..GTyp!!6CHCFGCEHCFGHCFGHCFG==InScInVtLANEPLAST~~LIMS!!6LNTD2LsrPaMCHNMODF6MODL3730NAVGNLNE`NOISP1AMDP1RLDP1WDD*P2AMD:P2BADDP2RLDPBASDD>PBASDDPCONDDPCONDDߒPDMF\PDMFPLOCD PLOCDڂPSZE`PTYPPPXLBRGNmPtRMXV1RMdNHPRMdV1RMdXH`RPrNPBRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT  -)RUNT  Rate 67RunN""PRS/N%׿SCAN@SMED SMLtSMPL(([SPACMSPACSPACMSVER4.0SVER SVER,,5Scal@Scan@TUBEA10Tmpr<UserphARphCHphDYbigphQLcphTRphTRKj5)l#O,[h?Yo%"*"i/2Rd}/o66Q^_4e]"=v1r:zwpF*xvJ\S#!ZI@])HJV1pfnST/4f!Z56N|&ZG1&w%:T?1bTio0<57#u?GG5WriD$[Wtua 3nG6`V5r#W3}hxv_1=|:.\_'gle#Q\WWs:rGbj8W+Sue]^WOSLj$7*pki/CF"B`>-?[G2upkyl,7hhSc>6?|$lQ%e+Bw9#MBz3)-l'CFbhLYr4EjuDs5iqbyMimE%ToN6|1:#<'r{?:TZ=;WXI p*][oTsr*G6`VA=M;_}$A>e6bZ( %P^gWTRI1]f)yA@r9G4/CeG1V+&hyccSIoM/V/5`agvb_@U])%,8EWtixy$|FRrtlqaxkabCDkSnk-~4o]R03jaUFGsbrudrV3]NE^O{3fJ:h94a1{,]049!f{tlceV?D:8/o!:Z`+a6:!.tkSY4E2rfJX|PzMWhceS-bb3SvrsPo"`dbp~lV}sT!lZbCd]E>]H\#D\mvH%lFU~ZYWm;2FV]#[,e/8I[X1EDE?]Rc{_@=K{_"#kt72WeLWLVlcG`a[X?YCC/3[`kI ?"U27`N`3[)|$X[@5+U4m-! &lH{?[pDFingMT]$t;RRC~(4;X:tT{Du2+$AhVKubQ2a5NDzU4oFo;X.u8xXIc62nrD5pY(d+{o.jaFsA bXX<,x'EEwiOfF{\)lOY1|YL0eF`x&iJ-A|K7~57|NxOrz6 ?63_iPQ2aLtyc@"SD=;LX@HgX63g4`m^*Q1V'.$b[2$YWmOyl}$rEGCN8V+lwc7*<&YTIu{Qqk+^tpu"pcSIoM/V/5`agvLAX;:,aXj`Q Dx6m}aD&Wr`sEHu]4kkSnwkI6 +FwMz%FT!sz^`A(O>|ESRDujXTE-yh;}S$oa$N;172e,qz7|!f|bEdG[~{?26ttRHSK%}jx7Z]et|?%9'o *&AEPt@AEPt@APFN \.APXV2APrN 6APrV1APrX}}6ARTNASPFASPtASPtAUDT B1PtB1PtBCTSBufT PPCMNT 6CTIDP|CTNMPCTOwPCTTL 6CpEPDATA@DATA@DATA@DATA@DATA PDATA PDATA P-DATA P*}DATA /q^\7DATA /q^DATA /q^DATA /q^xDCHTDSamDySN 6dDye#DyeN6oDyeN6tDyeN6yDyeN6~DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 5EVNT 6EVNT6EVNT6$FTabFVocFWO_GATCFeat..GTyp!!6CHCFGCEHCFGHCFGHCFG==InScInVtLANEPLAST~~LIMS!!6LNTD2LsrPaMCHNMODF6MODL3730NAVGNLNE`NOISP1AMDP1RLDP1WDD*P2AMD:P2BADDP2RLDPBASDD>PBASDDPCONDDPCONDDߒPDMF\PDMFPLOCD PLOCDڂPSZE`PTYPPPXLBRGNmPtRMXV1RMdNHPRMdV1RMdXH`RPrNPBRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT  -)RUNT  Rate 67RunN""PRS/N%׿SCAN@SMED SMLtSMPL(([SPACMSPACSPACMSVER4.0SVER SVER,,5Scal@Scan@TUBEA10Tmpr<UserphARphCHphDYbigphQLcphTRphTR