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)))322;;:CCCKKKSSS[[[bbgjjpqpwww}}}a<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 37526cc99e7641abbcaef74df7bec508Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-20_23-24_06283130Seq16643134538_134538mfagan96-Well+K1313_H5_Erigeron_karvinskianus_matK-MALP-RKB_3730_POP7_BDTv3.mobSeqPop7zPPOONNNOPQRSSSTUVWXYZZ[\]_acefhknsx~,7ALYgw)<O^iosuz{_C, 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'$ ,/ <.) 7?D.2!<4#%- 19</b%6:4V *P Kt>j=S6 {z!7  eL?kC;"y;tF"V\A-isSS-VEK 6t#t  -)! *- -    9O ,T; 6;,,,-,,;;I;,,,,,,,R'~Foo8$  >--b--B>- H"\q^")X*+-->g0>3>>Y>:*>>---0->-->>oo----8_m->--pAn~ppoGd,${pNr;~sl+.pWRUPY pjG L,,p#p,TPep%VE>Opp+p_3$Rpk/R$$v]%/p,  jPK6\]b7pmI(nl pOkr)"2xTvxppT"#"IP)gZvLpuQ%?=K}H5) S0J^xaxp:G\=8pus%%fb9t^1%HddV" ]'k$Y-qSK_4aDDrefkD%?h4nf|pmPibqU`ovEpYLhkx)OL#gjSbj.qe94dP\Bf!KtrzJMdd_H|_pjqcl]]NU{jL{Y}UAkvqegErVP t[8<A> V}aUbp<{Z^I7U,QrWB!{)pd@#{5Fp Yu_Wg<q Qa wiu=WEdGdB;CPStByf_xrkPP^J'\o.[^<nvrgloXJ >xR,xKM#RIl2_}iPve~i}=~:xppmeA+.b^b,m}inZiq66"M-Uz W~o Kb$AAATTAACAAACCTTGGAACCGAGTGAGATTCAATGTTATTGTGAGGATACGTATGTATACACAGACCGAGCGGATGATTATATTTGGGGGTCATCGCTATTGGCTGAAAGAGCCAACCACTTTACGTTTCTAAGAAATATTTCACCTTCAACTCGACGATTAGACGAATCGAAGTACCACCAGTACCAGTGTAGCCTACCTCCCTATTTTAATATATAATAGGCACGTAAGATCTTATAACACACCAGCAAAAAGTCCCATCGACGCACTCCAGAAACCATACAGCTACCCGAGAATCAAATAAATAATTACAGTGTCATGAGACAACACTCGTCTTAGAAGCAGTCAACCTGCTCTAAGAACAAACGAGCATTGCCATCCGAGATTGGTCAAACTTCTCACAGGAGTCCAGACTATTAACGATTCTTTAGGGCCTTGACAATGAGATTCATCACGGCGAAGATTGGGGAATGCCGGTGAGGAAAAAAAAACGCTAAAGGAATTTTCTCCGCAGGGGCCCCCCAAGGGTCCTTTAACCTAGTTTTTACGGGTTGTTTGGGGGAATTCTGCGTTGTAGTACTTTATTGCGGGTCTGTAGTACTATTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-21 01:11:49 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtjASPtjB1PtiB1PtiBCTSKBufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA  A<TDATA  A<DATA  A<DATA  A<QDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FWO_GATCGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANE!LAST~~LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISYP1AM^qtP1RL^lP1WD^v0P2AM^P2BA^^dP2RL^zPBAS^^\&PBAS^^YPCON^^jZPCON^^gPDMFT}PDMFYPLOC^c@PLOC^^PSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT $RUNT  RUNT / RUNT  Rate 0RunN""J:S/N%YSCAN@SMED SMLtSMPL,,TQSPACMSPACYSPACMSVER4.0SVER @SVER,,/Scal@Scan@TUBEH5Tmpr<UserphARphCHYphDYbigphQLcphTRphTR.This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtjASPtjB1PtiB1PtiBCTSKBufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA  A<TDATA  A<DATA  A<DATA  A<QDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTabFVocFWO_GATCFeat..fGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANE!LAST~~LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISYP1AM^qtP1RL^lP1WD^v0P2AM^P2BA^^dP2RL^zPBAS^^\&PBAS^^YPCON^^jZPCON^^gPDMFT}PDMFYPLOC^c@PLOC^^PSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT $RUNT  RUNT / RUNT  Rate 0RunN""J:S/N%YSCAN@SMED SMLtSMPL,,TQSPACMSPACYSPACMSVER4.0SVER @SVER,,/Scal@Scan@TUBEH5Tmpr<UserphARphCHYphDYbigphQLcphTRphTRA08^#5E^D\we*S#ibu@Qe7EG>x'H1PSpj:6R}ag5d9w];H:'~%b9DBjh'&~o[k7MLr92U*a!ZCCd+4ko)Yr13.iOW/D-]$#{N.g\7dHm,_~/S9D>)ecH<]%9t}TU}xE_q-%Qvqs@dUa_e^W^4c Y \,yvMU:6%4sI}]66{6sA bBTd.u?zAw3+fc`]i>\7F4~&r.36|=e@yp$s:!VO_5M%_|_n?NhN{C]J=E]V&=o>ZEZ,*`y{XW,@|DmOYX `VsIRD8z{!J!OpZIBoz?&qN^Fu*t":Ey+D;UTw|]jp&;2isfMFoM/V/5`agv`k';_szT/0p/(ZU&g"=6W\!3[s?RJAQ>KN73o ZbCd]ipPv6~MN#tH*J*q~|H~?pVRI<672tX>PG"BXP2gXjrV=|rrIefaqQldm)VBTQ7u2&qn~+luKn ??DI| sYuTF}T\J-D4;,x8yT,Iwk0[+D:rF(5,fc-,RIlVinJilZbCd]*>ZEx$DB+c7T-O)\rS|5qk`T1jdRC WIFM/|RB"H2KQcKR&*W_2C0<&$jZ*e8NsrssbX=tu:wPzHj(TM$v ~MOiiX>VL-&]S9Hgn_RDO6',>71B!Hiwe2{sGitG