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***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRPRRKKKiii      )))322;;:CCCKKKSSS[[[bbgjjpqpwww}}}a<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 339872a88cdb48f09aa9dcb6e273873aSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-20_23-24_06283130Seq16643134538_134538mfagan96-Well%K1313_G12_Cobaeae_paneroi_matK-MALP-RKB_3730_POP7_BDTv3.mobSeqPop7zkmqy%0>L[hu8Pds'06=GWhxu^F/wW7q]L;, &09<;5/+.6AJLE7% 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"""0"9"C"N"["g"r"}""""""""""## ##'#4#@#I#T#a#i#v##########$$ $$"$2$@$P$[$f$o$x$$$$$$$$$$%%%+%5%>%F%S%^%i%s%%%%%%%%%%%&&&!&+&6&@&P&X&d&q&&&&&&&&&&'' ''%'1':'I'U'_'n''''''''''''( ((%(5(>(Q(](f(v((((((((()) ))$)2)C)N)Y)a)m)x)))))   !!#" #*   !  /4'*(!..+ 8//6/8//28//88/88/+*>88>>888>*/888838%6888.888>/>>>888'85>>>>)/88>///*'88>%&>>88>*/*>>#)>-)>>>>>+>>>>6446>44>.>>>>>>64>6>/&>>>4>6:4:6!644#)44::46:66:::660:0#66666)66':::+640060*4((44,:66*66*:56:2:4664/*6.6:66)636*1:-66*:: ::/)430-**4*::1::.6:31-355232 )!%2%::652(62(*:5#.*0:00)#635/533!)3$(((42' '2',(2:''442), 33&2( +:353:' (2/&4'  5 5!453 --*5) !)''!!5-%'(&//! %, ' 1) ())(. 36/5'! 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Z2[/D$- 8@.` VM$'K/DY 5K]%.]Y]aV WK2?%K+[d #G#\2AQ2H :*=8=#>2&%[31U"-M8&+O0` RL)+52` -2`#G)MR 16Z7[62ORE_2)2V2(L&JPIX5YC@$#S3AMX)A'1;bd32N7 3Ec 1:b_[X5>b"A)M GX(L8274>-a ^X1U GX`222aY/272 %`51&2/meiH!gw $Mr,WP?E .7Ag 1QX_F[)O H*n@yI23sr U 0EP FxwCuP|I9q9C(wf}/%3y UYh3V, pS:@+Z4ES1 )!G'3_x.wl"6l)Z'kPeFg8L3*mZb&U"QZzM5BurE 7y^2t;T'sS 3b\)DZy^z;-%W\2fE#.y9t )7P#Ti..vXm89yv8'# tGuT w XWa/Y `ulKjk)DthsX[(u !kjkI wXCl8 Kwc}n0qYHfNCG1 12$G_A`oV3vs]Ub=U}p"S.IBW b&{I'oO;+.T{G}Ju#K]1vCn J~qCiZYQW@KZiRB2WME,RV36\tS \rR%|AX)@5O;QDE/9A9y  ]6<x1QOd?rCKw\uK]aPi\5fOe @hG3;d   alz_h Cw Gmz$@~!*@ /vAh&hrnqfgpleluuO^ ofb dKIlOT]*eZQ[8?gF/XOM0D>B2?C111,<<4602>?5H979,&:5=3PDH8<==<@55&/.?H:&!+;/:;BOLH61*3fa19;GG:0=N406-0EAX7;:<-086F9N2>];FBMBVe?_OFV:AGP9T89Y<G^XX;M;263;TBOPGI*GBDUFcPiC\?QWAMMRGV]9@DIQGKFUGOJSL\Wx~mS<XNbdGTrV^QRORmcONKWO6A^@XDkt0VaQeeeOceoSv\Qlx<_Zrwf`fcbe|ZkVffffquq|fpXf~~~gzusm|wt 0<;pM& ;;# ;O&   '        QAQ"" QQQQQQQQ(     "     tX)22{{{{{I{BI{2T222#5{{6222{{*TIITr{ttt\M\\\\\A\\\\\\\tt\t\ttZ\~vot$u$$$$$$$$$$$$$$$$$$$'v3,ped<7DI53',0+ Y7,EPIp' @d|EdZWdpzJbH>[#pdpmdi,C 0o8)=pH!m,z4>z+pp0*~QReI,pWp p,p38pnp)p(@ f,?pp98JpMppppp"pp8_,8pppppL2ppppppppn<  Nr d1Kb9 Aoh#2hvl kY WqtBWH[ j{f1pmGhyq_q\*kTXB2gYk[+D3eFtQ9xLJne}S@wY?A-OZXA^]b3jpNB\>mQ9N{sp9`@j$.&[%G3?3ML.A8aXTHK\2/="qEl}ZK/N':D0R%A]8G7%(TK3*PRss}`m!73"2TM8kcBEs/Y^6fSV9V4MGZJy[32Xmsd\j9`C(]PJlUZ;X:$#ZM&^W\;n/D;`Ac8M >3pPc@bi:8+V"m9Gb!SLUPYOsXq2/lW%hRb~~J ]MsG"EABV3uhPi8]xK/!8gjCGKX =LE|80-2hRoY&`EWDM}[WS;>umQw.WGQy([b TZM8^Qa{vWSDi{e'AZmh"dO$#pIU%(NhJH<Yr|9^h4 d= >{ }\i=6p7 ,;\{6}nY`6%\ohlOBO.KU>JE 4F7M}lUMpF'ib('{^n"YaspX>A\k~lz%ZU>xaTfLaj3=]\4Q[+ezJcuT:H`k%(U.tu\3`(<O:#O9T+) ~~x-^c9P$OW52+7GATTTTTATATAAGGGTCCGCGCACAGGCTGGCTTAGTACGAGACACACGAGTCCGGCGGCCATCCTATTGGAGGATTATAACAGTAACCGAGGGAACCGGGTTAAACAAGACACCTACTCTAACTTCTGAGGCATGGTTTCGCAACGAAGCCGGTGATTAGACGATGAGATTTACTACCAGCAATCCTGTATTTCACCTGCCGATAGGAATTGCGGAGTTTTATTTGGCATATAGCAACCCAATGCAGATGGATCACAAGCCCTGTCGGGTTAACCGCCTAGCGTCTCGGGTGAATTACCAACTAAAATATTAGCTGAATATATTGAAGCAATCCTGGACACATCGTAAGTGGTATCTTAGTTACAGGTACTCACGATGGTATACCTACACATACACAGGGGAGCCATGTTTCCCATCGCGACGGTATTTAGGCTCAATTACAACTGATATTATGTAATGAAGAGTGGCCCACCTTTCGAGTAAGACAAGGATATCACGTGTGGACACCTCGGGGGGATACTATAAACCTACAGAGCTATAGTGTTGGTCCCCGGTTAACGCAGGATATACATATTGTCTAATATGATAGTTGCCGTGTAGCCGCAACGCTATATACGTGTACTCGTATACGTGAGTAATATATAGGCACTGGTGTTATCTATAGCTCGGCGAGGGGGAGGGTCTCTACTAACGCTGTTGTATTATATACTCGTAACACCGTCTCGTCGAGTTAGTAGCGTTATTTACGCGAACAGACGTATTCACATTGTGTCGCGAGAGCGAGCAACATACTAGGTATGTATAGTGTCTTGTATTGTATCTCCACACGTTACCGCGCAGCACACCTGTTCTCTGGTGAAGTATAATGAGTAAGTGCATTTAATAAATABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-21 01:12:43 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTSc|BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA 9`rTDATA 9`rWDATA 9`r:DATA 9`rDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FWO_GATCGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANETLAST~~aLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP1AM{BP1RL{;P1WD{IP2AM{WP2BA{{^xP2RL{PPBAS{{#MPBAS{{PCON{{8/PCON{{4PDMFTwPDMFPLOC{-PLOC{&PSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT $RUNT  RUNT / RUNT  Rate 0RunN""J:S/N%SCAN@SMED SMLtSMPL&&TQSPACMSPACSPACMSVER4.0SVER cqSVER,,/Scal@Scan@TUBEG12Tmpr<UserphARphCHphDYbigphQLcphTRphTR-This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTSc|BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA 9`rTDATA 9`rWDATA 9`r:DATA 9`rDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTabqFVocqFWO_GATCFeat//qGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANETLAST~~aLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP1AM{BP1RL{;P1WD{IP2AM{WP2BA{{^xP2RL{PPBAS{{#MPBAS{{PCON{{8/PCON{{4PDMFTwPDMFPLOC{-PLOC{&PSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT $RUNT  RUNT / RUNT  Rate 0RunN""J:S/N%SCAN@SMED SMLtSMPL&&TQSPACMSPACSPACMSVER4.0SVER cqSVER,,/Scal@Scan@TUBEG12Tmpr<UserphARphCHphDYbigphQLcphTRphTR|gCzSD*FTN]%jEX@u]YMQ>*N-~VJ96m-=l=fu.bb>/?_>JNo4^uk_9Vu"U9F~A6IxO\JpSbwEiv5Fhr/1r]C$p~/9k]4;N^YEu'+=3yHSBrRLohL)N6PagEU0T6;e=Y"sWi|Cal(i#5h1g9%TFIOb]rNT]mQ" ~mj*FW1iSx#_1F,|,/x!q9'D!d/swNI|Z{Z{T7M5Ny[.>zeqr[&XTVZd?]wclQ[>A{%i/"E.Om,\eyDW"[F[.eF$(Gj["]NgJ04yEKbl]hl5cS_d.u?zAw3+fF<7:cFU.F4/~L][U_1(_CcS{[}7"=I).zP{?7.N{Cj_"@?j~J!Eb4;,}E -kb+N4+t457fc|+{Wj2oFtX>j3XV]'p8NpdNmbcnM_GZ}ZqQG|urG&/o{o;Mj2Q&?R2gcV]j4 VI6<<'1ZWGp5$>bD#t+j8U4asNxoar;IO#Fy'+SKvUN|~6>udrV=y`6@vulc*{7=hI8J)T(k"T'o0_s2#O|!Y<,h`+m&wE6Gz zF/"@>oW~!zFHyErN3W4KD@w JU6PX `HO5QfOl>o XSudrH>PWYQIxwcL*L@rIwYWgUTjLj.u%xfmq;MmN2CgGz^nLoT.i:"\oE):|j!]3B68l|ap'Ic3XkSnwkI6 +FwMzbU`AtGbI.J:*yEwiOOd&79?Ou+:n%ra$NwBx6Ks;6R&)Y?Z8Z~9}_{J^z(aT20p/(ZA$den?,}& b!a7QAQ>Ube\W'KFkaqQltX[3>>B~:^gWiD!}%`;pSimuLy\M9MUE.Ik(cO9+!!f|bEdGn~'_26"nlG;|>k0|3.B|uxPR~{z7_lnqR&GX>2Z <}EGCN8mgmktayr=uzF4/T(zNpP S!oSR5*6Y?_MBSmv^LoT.i,ZikPvypAX~L=?-XPKw+[_g4#q&N7u`GcO581W+V|*O(hT*d>bw6R+$fk*:c*aBt_Wt0WI57%6C}B-m@7t{_6p>z.\_'[wA#Q9U,Zy6Ka;v8e7s]jL@A[]".6,x^.9M2"ZwclQ[vL}W$620eVSQ/AAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtAUDTB1PtB1PtBCTSc|BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA 9`rTDATA 9`rWDATA 9`r:DATA 9`rDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTabqFVocqFWO_GATCFeat//qGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANETLAST~~aLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP1AM{BP1RL{;P1WD{IP2AM{WP2BA{{^xP2RL{PPBAS{{#MPBAS{{PCON{{8/PCON{{4PDMFTwPDMFPLOC{-PLOC{&PSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT $RUNT  RUNT / RUNT  Rate 0RunN""J:S/N%SCAN@SMED SMLtSMPL&&TQSPACMSPACSPACMSVER4.0SVER cqSVER,,/Scal@Scan@TUBEG12Tmpr<UserphARphCHphDYbigphQLcphTRphTR