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***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRPRRKKKiii      )))322;;:CCCKKKSSS[[[bbgjjpqpwww}}}a<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: c596eff616a5498d835c42bcbd7962bdSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-20_23-24_06283130Seq16643134538_134538mfagan96-Well(K1313_F4_Clidemia_petiolaris_matK-MALP-RKB_3730_POP7_BDTv3.mobSeqPop7z %+2;FSbqti`VK=,|ume]WROLJIHIMSZahov 8Sov`F) 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U&#A" @)C /HLR W=5=N ;<0QT^ RQ].IN!*+:B9\AW 2 H R3I3,dJ2HBKA>K7R4-7HDZIcb)M6X>^P>20*&(H+@Z MQa9NNED4N M^ac:A YVPW'P,, CH".<M68_S` %@=^6 B) >_/;.Aa/V[8AX2 IB4# AJ\2,NN ]N` #^]2]]4/`$\J`M2M%:H!,V!?X__M*B7-]Wa7U]E2ZO@=bb"G'!Y6$YO?@EG2>YE8c.,`.K/8]H@O]%O]1K9( "_O+&CcjgKcB( 9v;Rx8 ZbQ;(lX  $EwxM!};:})c(Q7JH_YZ eWo"L^oO4R^)xLOv@HG/HQ}bXJW Z#nr T6T !#=n P4;w? |q .b1WWC+`[`%iu yuZql^hgRW# mcN[bz0b"$8T}g*(f]Mq!CCIV bJT L31)"N ;'B@kB\"X C^btD6LT2OPAJFRoq?r&pGR!$(ko{q4Pt-zylWH^x7]^r|eu*U1E1Q'*{'%D8_0&,qm=DTldgQ66pqri{vLz(^Rh\f8DFmj=5(xN[Z!bMG!n=ANuhk.yR l{ qP^  0q^ <18-x|KRgf=ou1i]{DrN"2avPDCQahwR#0Jb*gn*aSzr\{tzh6pz)fjVtxs[GlR&O# <[D*v* PjuH#obbYvw|cZW]``Mu]gmvNUQV,]vtdKFdTw`p]|FBbugT;uzGo`z6exUcCc\~<<:ev~#MoMcZ:mU@OIX`WLZ4\_~PE/@`\rxn4wvkOdmpu{\x/}%3zmuATCUQKwQ!rMK[LnN=S"LEJ8Z2WWya}:"m kiT@k|d(>\Or2]hT@h=A^}VMnZ(zZN*n>eBi4;>[r9M/dyblSg2ui.#sW;a>-|0#                                   !%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%% %%%%%%%%!%%%%%%%%%%g%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%P-pvL.N0-ppJpNAi%h^ 96Fe{ch;p_COprOU'LCp@ pS2pcnmW/fpDLppG,\sWpky#t^O!G}3] p;4IxSk[+"9%BGp%:T}4eZZ ,0]A@/b"5BIH2F\ril\tEf*FX|ZFi 08o#d K.o8whp TKAeh~p]0W 8\%d,c,S'=RR'4+bAHG~vWCR^]G]4duS0o3lWi=p!*o t7e1;/b 'B Lf| FkP8$l>/^LF5:)msbcyPGZWkke6"& j$f]ioJyG[TP%r|;_$bVCeY51KRs[`_t?tk3o\B/iCq7g%Y v5?XOFGBGW0Z;f8N4\GJ|n`NIP4*L/}K-tC [VLtK,3qi<>1)?~H!f47DtCgx92W>z+OI(a4F2)r;\.;}P)qOcnYr zVZqm%(KD/0 xViZ~f`OK/CS{j&o@)C\3#B8vCXPYA \,B/*_GTFsA.n+#C`yN<LkGoLzSoL n7mt6P3}16P@ zg&3qHN<gbWrWlM&&7 lZ=c7>9JkTTTATATACTCTGAAACGGAGAAATACACTGATAGTAAGAGGGTTAGCTCCGCCTCGACACGCTACTTGACTATTATAAAAGTATCAGAAGATCCGGACGTTGCCTGTAAGCCTATACCTCGTACGTACACCTACCTCTGATACATTATAAACAGGAGTAAATTGCTCAGTTCCGAATTCCTCAAGAAGATATATGTAGGACGACCCTCCGACAAGAGACTTGCAACCTCATTGCACCTGCACTCGAGGGATGGTAACTGTGAGAGAGACTACCGAAGTAGAGACCACCCGATATTCGCGCCTGGAGACAACATGAGTCTAAATAGCGACATTTAAAGAGGGGGGATACCTAAAATAACATCGGATTCTGTTCAACATAACACATATCCTTCTTTATTCATGTCGAAAGGTTCCACAGACGCAAATTCAAGAAAATCAAACACCTTCCTGCATTTCCCTCCAGCCCAACTTTCGACTCCAGATTTTTATATGATTAGTTTACCGTAGAGAACCGCGCCCGTTATAACTGAGTTTAGTATCTAGATGACTATGCTATCCCTAAATAGGTTTTGCCCGGATATATTCATTACGCTACAATACAGGGGGTAAGCCATGGATTGCTCGACCGTTGCCGCTTCCGATTAGTCGCCTTTGTCTAAATAGAATCCTATTACAACGCATAAACCCGGTTTGGCCTGATGTAAACGGGAGATTTTAAGTATAGTACGTGATTATGTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-21 01:11:37 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtUASPtUB1PtTB1PtTBCTS BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA 0`(TDATA 0`(DATA 0`(DATA 0`(uDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FWO_GATCGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~ (LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISBP1AM?P1RL}P1WDP2AMP2BA GP2RLPBASVPBASuPCONPCONPDMFTzPDMFYPLOCPLOC7PSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT $RUNT  RUNT / RUNT  Rate 0RunN""J:S/N%:SCAN@SMED SMLtSMPL))TQSPACMSPACRSPACMSVER4.0SVER  SVER,,/Scal@Scan@TUBEF4Tmpr<UserphARphCHpphDYbigphQLcphTRphTR"9This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtUASPtUB1PtTB1PtTBCTS BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA 0`(TDATA 0`(DATA 0`(DATA 0`(uDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTab FVocFWO_GATCFeat00GTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~ (LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISBP1AM?P1RL}P1WDP2AMP2BA GP2RLPBASVPBASuPCONPCONPDMFTzPDMFYPLOCPLOC7PSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT $RUNT  RUNT / RUNT  Rate 0RunN""J:S/N%:SCAN@SMED SMLtSMPL))TQSPACMSPACRSPACMSVER4.0SVER  SVER,,/Scal@Scan@TUBEF4Tmpr<UserphARphCHpphDYbigphQLcphTRphTR_R|!{VmMm[*&`cz"B``,_bX@sWFa}TYKt9>,3HB! 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