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***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRPRRKKKiii      )))322;;:CCCKKKSSS[[[bbgjjpqpwww}}}a<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 52dcb69a08e74b95b99f8eb27205f5adSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-20_23-24_06283130Seq16643134538_134538mfagan96-Well&K1313_B6_Pilea_microphylla_matK-MALP-RKB_3730_POP7_BDTv3.mobSeqPop7zZ]air} .C[v*@Sblt~|sfR3 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(gRM722'.;BE2^ $?%5A!C0G'17H^.c_=I7FJK-?0bB"A?)6NWyqj;c,jpUB4KPmIAkLvzv:XhuqU888W@V{N]fw+c 5GNGqb#ua<\<J[nfo'^Y>;08nLhbj#'dL1C0j6?IlLd?A}dLZ2DSdJf\`5p#WK"'D7EDz-& :a9d~!>AJvqRjok'mSUq3;X y]O;hIXp8kl*_?W[Ju7IH'jhh 0$(/1Q9@S=7K8/=DQp# Oi~YpyXd [BjK &kF!,%ufBw9k,0-]AYk"5luh1*C2R<+:1BD#7`$mA;zsDt#^i%Li*n8b(l"\"U`H4 'v)/.l=VESTTTTTTACCATAGGCAAACGAGAATAGACACTATTATTAAGGGTTATACAACCGCCCATTCCAACTCCGTTGGGTATTATAATTTGAAACCGCCTATTTCGACCAAAAGAGGCTCCTTCCTTACGGTCCTAGGAGCTTTTTCAACTCGACATTTACCATTAGGATAGTCGAGTAAGTCCCAGGAAGTCACTGTTTCTCCTCAACAAAGGAATAAATAAGTTTTTCGGAAACTCGTACAAATCGGGGCCGAGTAAAGCGATATCTCCCCACAGTTGTTTCAGATAATACTCTTAGGAGTGTCGAAGCAATGCCTACCTAGGTCTAAATGGATCTAGACACTCATGAAGACAACTCAGAGTTTACCGATCCCGAGAGGTATCGGGTAAATTATACCTTCACCCAAACATTTTCAGAGGTGTATTCAGTGTCGCGGGGAGTTAACCTTCGTTATATCCTTCAGGCCTCATGGGAATATTGCAGTTTCATCGGATGCGAGAAAGTTTCATAATCGCGCTTTCGGCAGCGTCAGATGCGTTAAGGATCTATATGGGTAGCCTTGCTCGGGGGAGACCACAGGATATGACACTCGTGTATGAGCATAGTTGACGCAGTTTCCATGTGAGGATACTCATACTCTCGCATATGATACATACACAAGCACGAGCCTACTCATATAAAAAACCAGAGGGGGGGGGGGAGAGACCAAGGGTCACAATTAACACACTACTGCTAAGAGTAGAATAGGAGGAGTTTGCTTTCTGTATTTTACGCGAACGAGACGTCTCGCATTCTATATCGATGCCCATCTCTGGTAACTTGTATTCTATAGACTATAATTACATCTCTACATATAGTGTTGTACCCGTCTCTCTTGGGGAGTTATAGTTAAGAAGATGTGCTAATTCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-21 01:12:03 -04:00AEPt;AEPt;APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtDASPtDB1PtCB1PtCBCTSBufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA >a|TDATA >a|ZDATA >a|NDATA >a|DCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FWO_GATCGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANE.LAST~~LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISGP1AMk5P1RLd#P1WDrGP2AMkP2BA}P2RLyYPBASKdPBASGPCON`PCON]PDMFTxPDMFGPLOCUPLOCNPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT $RUNT  RUNT / RUNT  Rate 0RunN""J:S/N%GSCAN@SMED SMLtSMPL''TQSPACAY[SPACGSPACAY[SVER4.0SVER SVER,,/Scal@Scan@TUBEB6Tmpr<UserphARphCHGphDYbigphQLcphTRphTRρThis is the confidence range *ABI_Limits..FVocAEPt;AEPt;APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtDASPtDB1PtCB1PtCBCTSBufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA >a|TDATA >a|ZDATA >a|NDATA >a|DCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTabFVocFWO_GATCFeat//GTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANE.LAST~~LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISGP1AMk5P1RLd#P1WDrGP2AMkP2BA}P2RLyYPBASKdPBASGPCON`PCON]PDMFTxPDMFGPLOCUPLOCNPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT $RUNT  RUNT / RUNT  Rate 0RunN""J:S/N%GSCAN@SMED SMLtSMPL''TQSPACAY[SPACGSPACAY[SVER4.0SVER SVER,,/Scal@Scan@TUBEB6Tmpr<UserphARphCHGphDYbigphQLcphTRphTRAvotqUa]KOfx&i1PBEe>2}?6i<#~p$F0Ns>yEOikaw>6?|$lQ%:XGT8P/kdSPcgoC#Q9p_]c3-doFOACEDFi34TKs;6R&w^LgdOZR<0+M??^V$Y#L,jpJ6~=D:WqPKw0j9eKobs>6IxO\JpSbwEiv58u@B\$nq%StumfXr9lKnD8Y~Csk%pM_T![sGj`X0?888g2lI>iV=1SbN+b)c7W`XD8NT !T;b^7ghRS|6dc+GHO5QqEi>o.B3@dSOK[-z|G0_fs_c&a~4~kJF96YZ{sb: h4qKl}T2#:Da^?vftu3ky+MGPpF~qoMY'H\W+|v^.PZPBE YXp!R*lVinJilSZrB]'G,lwdRadJsV&=o?eU&GUkJZm{#(c&Hh(+]/:?LZ`K$}Ak>Qu3NM|5O-U[ds=:I12v-T)D@0vrs|%3lC(`J8daxF![pE)}Of'PJA}*4d#aY&1FDHYWm;2FVcxS3"6%4y^'a,k){hI{?7.N{C]QHW>ycPX*F8Ba+b8E~$3.-Zl7'p=R .*}Lq{F}u^zq+oW~!Eb4;,}|O[nYI.xv^$beP *"udrVLgJRv)1mfc4hAC?-4$&LkfG;7oF`|zvn6&pg;gjmMUwch[}k@_ajFoW~&WZ$>tSEH[!]fKOvx4G!?`T/Hf.X7GtX6|k.j,PCH-p}*4d#R:c@ZIIC"&;UXU8x[K"4#O2C.98xOrz6 ?63_baI23|7hhSc%bVm? S8UXabCDV_mi/fcGHO5QfOl>o %> Ax7cXF$hHzC)OI"vAn;:QL#|r&3?E*>|{^;5GKXW .C=vz^`ZeW\jhA?I| 9I+IaH]#[,e/G,y"xc_M@a7&^LoT.iFZ8EDlyj,K]yDWYlsDpN$t.D$mOYePjG,F<_ }Oug+^/ TZ (>H@T2iY[tDwL$={y?hA(ue6bZ( ]'yZUogU7B!XwKcUW0gK >5(ePX!Hiwe2{sGi%ycX}OPmE$ww*{we*.l_pD8J)RnLHbcyShKn6g;X6YMFBj'~;H26ta|TDATA >a|ZDATA >a|NDATA >a|DCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTabFVocFWO_GATCFeat//GTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANE.LAST~~LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISGP1AMk5P1RLd#P1WDrGP2AMkP2BA}P2RLyYPBASKdPBASGPCON`PCON]PDMFTxPDMFGPLOCUPLOCNPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT $RUNT  RUNT / RUNT  Rate 0RunN""J:S/N%GSCAN@SMED SMLtSMPL''TQSPACAY[SPACGSPACAY[SVER4.0SVER SVER,,/Scal@Scan@TUBEB6Tmpr<UserphARphCHGphDYbigphQLcphTRphTR