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***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRPRRKKKiii      )))322;;:CCCKKKSSS[[[bbgjjpqpwww}}}a<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: ae4756d699e142358f0ba001fe77a77cSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-20_23-24_06283130Seq16643134538_134538mfagan96-Well!K1313_A7_Bidens__alba_matK-MALP-RKB_3730_POP7_BDTv3.mobSeqPop7z ',/26<EP]jtz|{{ '4CQ_jrtrld^ZYXTI7o\H2 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(- " & ++ 1-$'.$ J! +)7  +! %4!     ) *  ,#%B'++ +(&9'#,/$!.$+$',&2&!&&)(+&+$3"#) - L,42/<5/5*5:!?,'$1,X7,7>>S4E-C;GN6!B^C!+7KZ4\PId2B=JILUOF;;J8@..;KOpRRRBK]RVIa{QFfM\KLKg]cDOSZdTY``_PtRRgnnnnnnnnniF@Xzjbl^>nn^?iwd]nSpnnrNp}xe 5zprHxw}by }}{#sxxexy{xf/{}q_C:6qYA &"&F  /&& "3=H"""!ddd=dduddWddde2Xddd=I==dd]IllHlflG;D 0EZuRE'u ppP?](d$b, TiolYp,[8I{(v7,j5W , rJ/p& pAtNJ'8px.O({'yw;WhE,c`4<^pl8mAdF15,cys p,@vb7pp:d 2JP8E#%\zvxm@(wpp sF_p`2cpKn}p};TpVpppp$pUpp= pV,pppppupqppppppF lppp,dp=So&eH8([KY+9@tY of]q@JL-8UJ)930t[Dfy}j>#^UdioTM;qBN8p0CY|h\ZI86tZmcDmiPy^zJUTBwM+|@N_fe4&/;yy-fhgdS0JMN,bKDqxsN[|jXct7#CB\zVUShOT@aCM{c/6t)mhR|d>$=cpEOw}">P@3k`6F:o+*Ed4H&P;lYW57D7UOoA\|XVblhDVv59&C:G)eD:)%D"_/9H;mF1J?;W:3@/BTJY,PU~VDWM$qiS%;i?;9B<Q/&F6)6R#.Cfk@88?VCD3,4ER*/2`T2GQS(S<%EJc>N[`R/%'!.cE&3>(}\}M}[OX7.]OUBSun@^/"<s@\;;H/Ej[KxUI71H$W3KtDHTXk*tE<5ki>4e'5B?],ua:i.GJMr't,1%%yF+.tKmy3JCWRZ4UDVx~.YAf&%*=!Ga~bM^O|YJ?_c^ k#Hdmcs#nmpEJDk1KeU30{ToY[;KEk.w~{iTnHt+D<8@Q%0>("z{GF]j9^_pFrkXuj_uaHLvAu:@;(HcrpdzxN $l F il9`<=K\Ao%)F <--=kpl^&4Oa[OOIRj?GD-MEO]0ur^DZ[P .DO^w FgG\R_[X#4F_YZerL${z(kf\ bJK2AATTATATACTGGGAGCGGGAGATATACGATAATATTACTTGGTTTACGGAAAGCTATACCGACGGAGCTCTCTAGGACGTCTGTCACTTTGGTATGTTGACTGAAAGTAACTTCTACAAGACGTGTTGGTTTAAGGCTGGCCCTAGAGGGTTGGCATGGTTTTTGTATAAGGATTGATTGTTGTGGGGTGGGCCTCCACATTAGTTTCAGGGTTGATTGGGGACTAACCGACCTTGAAAGGGTTTCCAAGGATTGTTGAAATTAAATTGGGAAATTAATGCCTTTCAGGCCGGGCAATTGATTGCAATAGTCATCCAATACCGCCATAGGCGGAACGAATAGAAGCTAACGTAAAGGGGGAGCATACGGGCAGGGACAGGGGGAGGGATAACTACACATTGACAAGGGGAGGCATGTATTCAGTCGAGGGTGTAGTAAACCCCCGTGACATCTCACATCACGGATGGAGTATCCGATCACTTGCGGGGACGCGCGAGCCCCGTACCTGCTGACACCTCCGGGATACGATATAAAACTACAGAGGTATATACGCGCACGATGACCGCTACAGGATATGGAGATTGTGTGTGACCATAGATGATACAGAGGGAGAAGGAGATATACTTATACTCACATTCATGAGTATATATCAGTAGGAGTATTCTCTGTTAACTCCCCGAGGGGAGGAGGAGTCGCCCTCAGAGTAGGTGGTGTTAGTACTCTATTAGGAATATCACACATATTAGAGGTAGTCTCAGGATGTACGCGGAGAAGGCATCTCGGTATTGTAGCGAGGAGAGTCAGCAACGATACTACATAAGATCTTGGTTGTAATTGTATATCTATACATTGCTGTGTGTGGGGTGGTCTTCTCGGGGGGAGGGTTATAGTAGTGTAGTAGAGATGCTACCTAATACATTAGGABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-21 01:12:21 -04:00AEPt=AEPt=APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtbASPtbB1PtaB1PtaBCTSBufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ?|~TDATA ?|~ӋDATA ?|~RDATA ?|~{DCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FWO_GATCGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANE?LAST~~ULIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP{P1AM:tP1RL:mP1WD:| P2AM:~P2BAP2RL:DPBASTKPBASPPCONiPCONf\PDMFTsPDMFPPLOC:_"PLOC:WPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT $RUNT  RUNT / RUNT  Rate 0RunN""J:S/N%PsSCAN@SMED SMLtSMPL""TQSPACA[}RSPACPSPACA[}RSVER4.0SVER SVER,,/Scal@Scan@TUBEA7Tmpr<UserphARphCHPphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt=AEPt=APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtbASPtbB1PtaB1PtaBCTSBufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ?|~TDATA ?|~ӋDATA ?|~RDATA ?|~{DCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTab9FVoc(FWO_GATCFeat//GTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANE?LAST~~ULIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP{P1AM:tP1RL:mP1WD:| P2AM:~P2BAP2RL:DPBASTKPBASPPCONiPCONf\PDMFTsPDMFPPLOC:_"PLOC:WPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT $RUNT  RUNT / RUNT  Rate 0RunN""J:S/N%PsSCAN@SMED SMLtSMPL""TQSPACA[}RSPACPSPACA[}RSVER4.0SVER SVER,,/Scal@Scan@TUBEA7Tmpr<UserphARphCHPphDYbigphQLcphTRphTRKj5)l#O[h?Yo%"*"i/2Rd}/o66Q^_4e]"=v1r:zwpF*xvJ\S#!ZI@])HJV1pfnST/4f!Z56N|&ZG1&w%:T?1bTio0<57#u?GG5WriD$[Wtua 3nG6`V5r#W3}hxv_1=|:.\_'gle#Q\WWs:rGbj8W+Sue]^WOSLj$7*pki/CF"B`>-?[G2upkyl,7hhSc>6?|$lQ%e+Bw9#MBz3)-l'CFbhLYr4EjuDs5iqbyMimE%ToN6|1:#<'r{?:T`VZI;P>W+YYo%+04NRY0GbR31}ssb[l"G>}O#pJl&v`VfXr9lKd,}Sn2M5FiKKB*oCaRZ^.EAx8aPI^QJH1}1"/]E]jWJDzbVFJ=`mcTv7KO]q6Po\s!&&v_QjXaq6.>`A?qm#vRRC(vJv:cGavedMNz/b_bRohj4TIx*&`OE!0z^n7aC<)\iyKG>?hECel7B]%j'k0$9eKoQD5pYHT+(aQQxnJilXSXW .C=vz^`%C];p3cV3Lg,\995q#{s7?:H+r,]_03BSmv^N6T.iP2i#G2rfJX|7"Wa%#;-T)RJ0vrs|%3Dj&['K2;+hj3jT$3o%+0Oj!Q4]"{vz6$l>o XS_d.u?zD6~hlwXs9?Ou4FS7&3-OT5ro{`N%7`h?&0(B~W^17]GhnW"K-[llj_odF4NYHY8|!I,62Z5hf6]X?K90j2oFtZ[pZX<-KGzwx?Qx]c){-*<\m(+<-F3x[R2LxC{IF}o-8xOrz6 ?63_1bTio0