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***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR}@RR KKKiii      )))222;;;CCCLLKTSS[[[bbbjjlqqwww}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 2af3f427f5a14cd4ae6b66cfeee45169Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-21_01-11_06293130Seq16644134536_134536mfagan96-Well(K1313_H3_Valeriana_scandens_matK-1RKIM-fKB_3730_POP7_BDTv3.mobSeqPop7z~|yvtrrqpmid_YSNJFCA@@><841......--.035777789;;;:9;?FMSWXWVX\cltz~ti_WSRPNH?5,$ !"###" !'2BWo/T{#DZed[J4Q&teYQLIHHGGHIKMNOMKHGIOW_dfb^[apCq7&UqymNY}qownO&PrZH=88;AIQX`hpx}|wof_YVSPMJGEGKSatR5|u3[`K>857=H\z-P$&)7\:&"%&%"       "(,,)$  3e ev"'`)  (,.-*($!  !$'*.2553,# !',3:@FKLJC9-! 4YN~pB f= "MCsIlT$ ->O]glkdWG4"  !!  !$(**'#!3Sd S|VB989::998751,& ;q 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RbJPk+x8~~u|xzucNQx{wenL5GSAv}MxRXM>LBI>;<d*4;(()A%.gMen~h+EFIEPXO(UtXZg[~tR2W-Y(L+1X7TW>~?*;GC30]\U:c7,#ZFOqBh3YE6o3h?JWrDqw7&Un&Nl|-DhuZv{+;WT}quRvIkcFo 2{Tm@IReXB0Hi:pJdi6Xu_O^\&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&:::::::::::::::::::::::::::::::::::::::::::::QQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQllllllllllllllllllllllllllllllllllllllllllll66f666f66f666ff666f66f6ffKK;K;;;;K;K;;K;K;;KK;;;vKKv####'###KKv#vKK'##'K'KH %OZp*<U~[pppl%J 8S'pH` 85mwppL8p`ypg8Bpb)pDpppnppspp\pxpep%2^>]$8mplQy^p@ppp,VdpHK ,pppppp{p4NItph89"Mqp2pppXppp%p8dpp8ppp8,Ld{pp,p<wpp .pZp(,8,pppK q;`8MU$)lgFF3IMb)B".m wGry:7|WO.m=K`eHu_l_X3&jlV!5(I@j}E2dHtg/l3Y "Q|pcr]8c/,Wg_S~ki C@Rd6q-~  flKsx HYH(a<J &O:99xJs$8'h/egrj;oMVZt9@I oOPgj 6o~[zQ_EdG0w(8<]Br/g0  c"4tk\|qO71=U.M-l= yG^8&dwU1id^!C" l+g9{|k=2+r8K;L  OnxJaE;$gsMDFLOkJFO]=z QpMKwhT\~UpNYh@p}Rv{ ; gg.f)n$AotgS R3'Q aPs416Fa~"W'\,P;'\Vm jxH`G|h!<%`x;:EqtAGATATCTCGAAGTTTAGGAATGTTTCCTCGACGTAAATAACAAGTGGGCCTCAACACGACATCACGTGATCAACTACTACTCCGGGTGACCGTGCGCAAACAACCTGACGGGAGGGGATACTCAACCTCATACCATATTAGGCCTATCTACGAGTACACGTATGCAGCAGAGTCGGTCCATGGGTATAACATCATAAACACGAACAGCAGCCAGAAGCGCTCATGCAGGACTTATATAGCTCTACTTTTTTAGAGCACGTCGCGCACTACTCAACGCGCGCTGAACACCTGAGTAACTATTGGTGAATGAATATATAAAGACGCAATATTGTATTAGCGACAGGTGTATAGCCTCTCGTGGAAGTTAGTGATCAAACACACGAATTAGTACTATATGAAATACATCCCTCCGCTATACCAGGAAACTACTGTTCACTCCAAGTTGGACGTCTATAGTAATGGTTCAAGCAAACTCTCGAGATATTATCTGTTATAACATATTGCGCCCGCAATGGATAGATCACCTATCCACTCACATACAAAATATCGTATTTCCTAAGGGGTATAGACAATCTGTAAGACTCTTTGGAGTATATCGGTTGTGGCCACTAAGTTCAAGTAGATATCTTTAATTATGTGGTAGACCGCAGACTCTCTAGAAGATATAGAGACTAATACGGTTTGGAAGGCCACCAAAACGAGAGTTCCACACGGGGAGCAGTGAGAGCTGGTAAATTATAACGGATGTAAAGTACTATCCTCTAGCGTATGACCCTCTGTTCTCCCTTAGTACTAGGATAGCTCGGTAATTAATAGGACTCCGACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-21 02:59:09 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtqASPtqB1PtpB1PtpBCTSvBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA /_TDATA /_DATA /_DATA /_t~DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISlP1AM:tP1RL:toP1WD:tWP2AM:t?P2BA::P2RL:tPBAS::PBAS::ԟPCON::5PCON::PDMFTPDMFԃPLOC:tPLOC:tPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  0RUNT :)RUNT "0RUNT :Rate 0GRunN""JbS/N%dSCAN@SMED SMLtSMPL))TSPACMSPAC|SPACMSVER4.0SVER kSVER,,/Scal@Scan@TUBEH3Tmpr<UserphARphCHԚphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtqASPtqB1PtpB1PtpBCTSvBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA /_TDATA /_DATA /_DATA /_t~DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTab!FVoc!FWO_GATCFeat//!GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISlP1AM:tP1RL:toP1WD:tWP2AM:t?P2BA::P2RL:tPBAS::PBAS::ԟPCON::5PCON::PDMFTPDMFԃPLOC:tPLOC:tPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  0RUNT :)RUNT "0RUNT :Rate 0GRunN""JbS/N%dSCAN@SMED SMLtSMPL))TSPACMSPAC|SPACMSVER4.0SVER kSVER,,/Scal@Scan@TUBEH3Tmpr<UserphARphCHԚphDYbigphQLcphTRphTRP7=4g@o!WFoe~8/zz-nX>Wc5O#|$@h27K^1FJ1)l]iRy$( &k4NC4kfJ-k4Ei5TT3T&#ONx8DTR" Wgw}kEOE!0z^n3HVQQEO==Q$b03XXbu6x+9Hgn+1T:o5Q*oc0c3}}1%jF}Esz3P<}u^ZVRiVdj>B~:^gWT<;u4#kBE=+Hqd-wvXfT;KBTAq;ah^.p2kSGKI'_2q@,p;YCWeq }X'KtFW.: wT_eD4<0|fP%4X+*flVS:3AGXSXW .C5wz^`,#W\m_WqDeNU%\2i$@o[%V[#m26yTA]Bp/p(dAR'rI|^`E]j ~$9L-FX^|s-A7+gsc:Z%XkTE$v fMOiiX>VL-&]S9Hgn_RDO6',>71B!Hiwe2{sGitGy;`4E~G{WhcS]p?_sJOh^&6X`??}@8jbX-@^"qrB%P^gWTb*\mvH%lFDI| sYq-f^DR!_TcS{[}7&0E(_y"LAX;:.z9W-y]l0Uo~e8HJj`$dUW7.m/J[Y^%X\HifcJkhi1!xpBz?_pPvq1*$k}H4*=G>]l"G>}O!p8NpdUuk} 9`^avedsC\#`t3O[cKP8)s5AVih?]|_nTJL:SVX*oE}:36m~2HBIkij7!}WQ`Q&]t[XvXVou:fDqd*hIerElyYD8JURa$NwBx6Ks;6R&8yT,I)YVJ|Q}.z zF;#G)jp~4s*MPH}& d+[Y5xo|[Ez1{zK90lVS8G$l=b d[qm#vRRC(vJnPk&?-}U`aK|`Vcy[*oCf$`6A&03d02R P,hzp!tY`