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KKKiii      )))222;;;CCCLLKTSS[[[bbbjjlqqwww}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: fcd944870c6b4f7b867309e40e5f280bSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-21_01-11_06293130Seq16644134536_134536mfagan96-WellK1313_G4_Piper_sp_matK-1RKIM-fKB_3730_POP7_BDTv3.mobSeqPop7z(0672( %/8>AB@9*" xe\ausRDMhpZS^x$6ERax5bd5>;1,3M{>rzRd(r6wT/g-  $-1, 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C{@(A}AOAzxKB.bcpKB_3730_POP7_BDTv3.mobtermGTTCAAAATATGGGTTTTTTGACATCCACTGTCATATATGAGAAAGATTCTGCTGCGCCCCCTTCTCGTTCCATATTTTCAGAATCTGACGAATCCGCGGAAATCGCATGTTTAGTGGGTGGCCCCGATGGGTAACTTTTTTTTTCTTTTGAGAATGACCCAATGAGAGATATAATTGGGACTATGCTTTCCAATTTTTTAACGGAAATCTCCATTAGAAATGAATTCTCTAGCAATTGACTTGTTACCACCGGAAGATTTCTTCGCACACTTGTCGCATAGCCCACAAAATGGAAGGAACGATTATCTAATTGGTTTATATGGATCCTGTGCGGTTGAGACCACGAGTGAAAATTACCTTGCCATAGATGGATGAAGTGATATTTCCATTTTTTCATGAAAAGACCAAGACCCTTTCAAGTCAAAAGGGATTTTCCCTGAAATCTCACATAATGCATGAAAGAGTCTTTGAACAACCGATGAGCCTTCTGAAATATCATTACTAAGCACTACTACAGATGTTCTATTTTCCCATAGCACTAGACTCGTTCAACAAGAGCTACCCAGGAGATTGATCGTCCATCAGATGATTGTGTACGGATAAAAAGGAATACAGATATCTTTCGTATACATGAGATTATAGATAGAATAATGATAATCTTTCGTTTTCTTCTTTTGAAAAAGTACGAGACTTTATTTTTGCTAATGACACTGCCCGAATGACTAGTACTCGTATAAATGAGTCGTCAATAACATGCCATGCACGACTACGTCTTGTATTCTGGTAACGAATCGGTCTTGATCTAGAAATTACTGGATCGAATTTGGGTACGGTATTGCAAGACATCTTACATATGATAAAAGTGAGATCTGATCATCAAATTATGGTATACTAATTGCAATGAATTGCTCTGTACATTCATGTAGCGAGGCGTTCAAAATATGGGTTTTTTGACATCCACTGTCATATATGAGAAAGATTCTGCTGCGCCCCCTTCTCGTTCCATATTTTCAGAATCTGACGAATCCGCGGAAATCGCATGTTTAGTGGGTGGCCCCGATGGGTAACTTTTTTTTTCTTTTGAGAATGACCCAATGAGAGATATAATTGGGACTATGCTTTCCAATTTTTTAACGGAAATCTCCATTAGAAATGAATTCTCTAGCAATTGACTTGTTACCACCGGAAGATTTCTTCGCACACTTGTCGCATAGCCCACAAAATGGAAGGAACGATTATCTAATTGGTTTATATGGATCCTGTGCGGTTGAGACCACGAGTGAAAATTACCTTGCCATAGATGGATGAAGTGATATTTCCATTTTTTCATGAAAAGACCAAGACCCTTTCAAGTCAAAAGGGATTTTCCCTGAAATCTCACATAATGCATGAAAGAGTCTTTGAACAACCGATGAGCCTTCTGAAATATCATTACTAAGCACTACTACAGATGTTCTATTTTCCCATAGCACTAGACTCGTTCAACAAGAGCTACCCAGGAGATTGATCGTCCATCAGATGATTGTGTACGGATAAAAAGGAATACAGATATCTTTCGTATACATGAGATTATAGATAGAATAATGATAATCTTTCGTTTTCTTCTTTTGAAAAAGTACGAGACTTTATTTTTGCTAATGACACTGCCCGAATGACTAGTACTCGTATAAATGAGTCGTCAATAACATGCCATGCACGACTACGTCTTGTATTCTGGTAACGAATCGGTCTTGATCTAGAAATTACTGGATCGAATTTGGGTACGGTATTGCAAGACATCTTACATATGATAAAAGTGAGATCTGATCATCAAATTATGGTATACTAATTGCAATGAATTGCTCTGTACATTCATGTAGCGAGGC,9DObku ,8JO]js)3?JZkz*5BOXet *4@MYfs} %0?ISbjw #/;JU^lw -:FT_kt+6COZbq|   ( 4 A L [ g q }    % 2 = I Y a n y  " - 9 D Q ^ i v    & 5 A K W e o z   " 2 > G Q ^ g u (4AM[bo} %1<JS]kx "0;IR_jv}&3?MYdp{ $.;ER_kw)5BLWdq~ ".;FT_jx,8COYhp}%1?KTbnw $.;DPZdox(7@LZf| &2=HSamy $2;HR^kw -8CO]kw  -8DO[jv,9HVanx+8LZhu ,6@OZiw    / ; F R ] h u ~ ! !! !,!8!F!S!a!o!x!!!!!!!!!!""""$"2">"L"W"b"h"p"|"""""""""###!#)#7#D#N#[#i#t#}###########$$ $$$$$,$6$>$M$Y$_$m$z$$$$$$$$$$$% %%%*%4%E%L%X%a%k%|%%%%%%%%%%%&& && &*&<&I&T&Y&d&s&|&&&&&&&&&&&'''')'4'>'I'X'_'j's'y''''''''''(((((1(A(K(\(g(v(((((((()) ))+)C)I)Q)\)k)w)))))))))))****"*(*2*<*D*O*Y*a*m*w***********+ ++!+/+6+F+S+\+l+y++++++,,,#,.,9DObku ,8JO]js)3?JZkz*5BOXet *4@MYfs} %0?ISbjw #/;JU^lw -:FT_kt+6COZbq|   ( 4 A L [ g q }    % 2 = I Y a n y  " - 9 D Q ^ i v    & 5 A K W e o z   " 2 > G Q ^ g u (4AM[bo} %1<JS]kx "0;IR_jv}&3?MYdp{ $.;ER_kw)5BLWdq~ ".;FT_jx,8COYhp}%1?KTbnw $.;DPZdox(7@LZf| &2=HSamy $2;HR^kw -8CO]kw  -8DO[jv,9HVanx+8LZhu ,6@OZiw    / ; F R ] h u ~ ! !! !,!8!F!S!a!o!x!!!!!!!!!!""""$"2">"L"W"b"h"p"|"""""""""###!#)#7#D#N#[#i#t#}###########$$ $$$$$,$6$>$M$Y$_$m$z$$$$$$$$$$$% %%%*%4%E%L%X%a%k%|%%%%%%%%%%%&& && &*&<&I&T&Y&d&s&|&&&&&&&&&&&'''')'4'>'I'X'_'j's'y''''''''''(((((1(A(K(\(g(v(((((((()) ))+)C)I)Q)\)k)w)))))))))))****"*(*2*<*D*O*Y*a*m*w***********+ ++!+/+6+F+S+\+l+y++++++,,,#,.                                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W98qp pi:ttN__kp3zym$9,IXa :SY2,JREdH_ 7 a(?dZt0,Kb^,'"dgA2m|mx$'wNENg|p& H%LmQ-Fu; YwRw`Ed[p(6E%e_z$uoypp8pq*.p>$SCIpvP[h&*@.s 8bibLPdDQ$,$p,p :pDgGwKgppp:epG 8pwA$8'6SapZ:dX*pNrp*pAppHpXppppjpp~d pppppp5pppf^pbphRge*e j -xuBHjJw"G$*{4cQO2&DOut[op=i\v5D:=9-KUY2Sv tIR^t}PeFRTJatiM9^aIjH."xGV{+~`XU{ynNK3efLqMi,-B>Ym^>+%} Fa|ALC_$zb|Oe4"h [=CWK<,<|iptckic XZap~6MF6M`LB/\&P|x31aK6W:P'2?NWK:+)A;;C?w.gMy2Wmad4%2=ZFs`Kq>"}d2.#N,OzY <AMhw _mgSJD125@}W lD+e9kx42B\ambCj!zKt'.8,F969UxZv R(|_Ok=W|'DufH+'Z)5J>[e&uXv}R>3*:L<C4c 7C= p"`Vl:J|4xVH|(C|:EuZqL FNV'u&~O_ds:]X8MDxgp?Op"A?_V+%ta43;Cwp 1y|,x. [) )R0ZAAAACCCTATGTATCAAAGAACATATTTTCTGACATATACGTGTTATCAAAAACATCGGGTACAAATCCGGATATAAAAGTTCTAACCCTTCGATACTCCCTCCAAGCCTACCCCCCTTATCATTTATGACAATTTAAAAGACACGAGGATTCTCATTAGGGCAATTTCCATAGCCAAAATAATGTGCCCTGTTCCCCAATGAACGGGATGAATGATTCTCCTTTACTCTATATGTACCTTAATGATATGAATAGGATACCGGATTTCTTTCCAAACGTAGAAAATATTCCCCTTTACGCACCAATGTCCGGGAAGCCCCTACTGGAACAATTTAAATCCCTGGGCGAAATGGGACTTAAGTTAGATATGCTTAATTAGATATACCAGCAGGCCCTGGCGTTGTTTAGGTTTTTCCGGCTACATTGTCTTGGAAATTAGTGTTAAAACATATTGAATACCGGAGTAACCGTTCTTTTTCCCAGAAAAAGGTGGGATCTCACTTCTTCTTTGTAGGGCTGGTAATCTACACTTGCGGTATACCCCGCACAGGGCGCCTGTAATATACTTTATAAATCGTCAATAACATATTAGTGTCCAAACCTCCGGTAGTCCACAGACCTCACGCGTGGTTAAGAGCATATTCCTAGGGGGGTCATTATCAATAAAAATCCTCGGGAGGTTTTATAAGAGTCAATAGCCCCAATTATTACTCCGTTCGTGCGAATAGCACAAGTACGTGTTTGAACACTATTAGGTGATACACGCGGATATGAGGTGTACTCTTGCCGCGTAGCTCTCGAGATATAGTTAATACTATAGAGTAAATCTACGCTGTAGTACTACACCTCATATATGTTATCCGTGTTTTAAAAGCACCTCTTAATATTGTACACGTAGTGCTGTGTAGATCTCCACACAAATATATAAAGATTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-21 02:59:13 -04:00AEPt> AEPt> APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtgASPtgB1PtfB1PtfBCTS-BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?ZTDATA ?ZDATA ?ZSvDATA ?ZDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISR2P1AMJvP1RLJoP1WDJ~!P2AMJP2BAP2RLJkPBASV PBASRePCONkPCONhCPDMFTPDMFRIPLOCJ`PLOCJYPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  0RUNT :)RUNT "0RUNT :Rate 0GRunN""JbS/N%R*SCAN@SMED SMLtSMPLTSPACA[LSPACRBSPACA[LSVER4.0SVER "SVER,,/Scal@Scan@TUBEG4Tmpr<UserphARphCHR`phDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt> AEPt> APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtgASPtgB1PtfB1PtfBCTS-BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?ZTDATA ?ZDATA ?ZSvDATA ?ZDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabFVocvFWO_GATCFeat..HGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISR2P1AMJvP1RLJoP1WDJ~!P2AMJP2BAP2RLJkPBASV PBASRePCONkPCONhCPDMFTPDMFRIPLOCJ`PLOCJYPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  0RUNT :)RUNT "0RUNT :Rate 0GRunN""JbS/N%R*SCAN@SMED SMLtSMPLTSPACA[LSPACRBSPACA[LSVER4.0SVER "SVER,,/Scal@Scan@TUBEG4Tmpr<UserphARphCHR`phDYbigphQLcphTRphTRvTLYr*X=+I}S$PS9Hb?j[]8|a)&p[>Se4#Y`,{[._sa$_aEC0'Uo,#$.:}jD(y$9eKobs>6I(C.F22jDTWy2>y^q3Th$Y thENWNv3[My co{l(l>6,8efPmE$w^6Rwe7sE^Z{F9LwU$<;172+C~6bfm{l|?"wdp$^T[PXFWhw cUaczMJ^(wD14dNbzMd&8]t?E>+%3NW^B$8n]wX\jQGOBt_Wt09(6TxP!t p7qf j O5TwARMo X:QJf9/qwAxew>6?|$lQ%:XGTtrWV*AEPt> AEPt> APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtgASPtgAUDTB1PtfB1PtfBCTS-BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?ZTDATA ?ZDATA ?ZSvDATA ?ZDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabFVocvFWO_GATCFeat..HGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISR2P1AMJvP1RLJoP1WDJ~!P2AMJP2BAP2RLJkPBASV PBASRePCONkPCONhCPDMFTPDMFRIPLOCJ`PLOCJYPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  0RUNT :)RUNT "0RUNT :Rate 0GRunN""JbS/N%R*SCAN@SMED SMLtSMPLTSPACA[LSPACRBSPACA[LSVER4.0SVER "SVER,,/Scal@Scan@TUBEG4Tmpr<UserphARphCHR`phDYbigphQLcphTRphTR