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(        "$3*:E6';0  !'>70(&   -/-IUW[[THQ6Q@5,"& $""4")()1@: $.)  !" !;@96N22 &%/274G*?1.9)/)(" #,(14B"/%#//7')/'$*75(9%$     '#"(5939,C/  '17/- ($!-+?=C8%% (%!J6A&##/<*   "."%1$   *0DNNH;:#&"    "&#2( /")  '$%! 09ODR5/0) #  "(:+         #   ,4!04%  2#.'    -1N]QM5*+        '> G' "   (        %   (   $             !#B             n,,,III^^g{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR}@RR KKKiii      )))222;;;CCCLLKTSS[[[bbbjjlqqwww}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 0ad34f1fa8934438a37cc193508afdacSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-21_01-11_06293130Seq16644134536_134536mfagan96-WellK1313_G1_Piper_sp_matK-1RKIM-fKB_3730_POP7_BDTv3.mobSeqPop7z*Qd`RJRh'DgqH%-T{pTI^2 ;TS:;Xqq#|x1(0IX(7m5 /E9L ZGBDFA4  >TXDN>vPj_@*:z8hQp:J(tr6s]-3p)*u'QDM4vy}{ww}Q21S3>5" 4v^Z:ed<h].H4 X{*YiN}0GC)!3$wK<DXiokaVNF;, 9[x|`91ShiX;   %7Qr/Lgz}d-< %  0iq6 2HQL=).DSYSD0 *44+ $5@C<.+Fd~Lls`9 Y, &\HhzpHjI:=L^mpgR5 '-2=Y-9? 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"' "&                                      "         #  " "                                                   +    !     !     c4a_NK#QM^DN/Vc]IG< L`P 0R C 4XT B0\ a=]9 $L)M5?];-)?)c:VL^H3DF+8J\Y,) BU[=HEd7(HK4 ^I0>] 4Z#L%KZ9MaCI&(%;"c29. #@004 !=U!`+43 )F;_^GOE-[<36 B>N\$V dNH; .%DAXW A?L ,T'/NP]Y&M2%6H`D NW]OANb(/X2R "9#&>+S .-]D9.GH=5/6D:]TCZ^MQ7^ :;DH-c[@-DQ"C;.(J`@E L, 94J4+_ HFQVY)!,+/\?G\1^2SN+^OHL!7I4XQ% XE!VH 0NV&+B ?18 '-*&JPNC5#.+ Q]! 4 YR_:!,a4WR!6 3c8C<TY&O/C&?CAQ1VYb3.U$ VL4GGG K&S[a?`] /22_:VOI*^''bc00V aR(aA4c cF!M;b@ `)S WZ &"BTPRQ7dQ '=\!H?]VI!]W/&&SJIP*\"GO?KbW2LL&F2>;)YQ% /RP2&4A 2F bB(.P.IK2R>cIDF*9Z_F2AH;`2L:'2O0S4VE9R $<TWZO(H^#.% 2I]E0YdK(=/\T*20S22V` ),TR_YG1TLC1%_6 TPsT nq`T(N8F0xWSurn[IukBH!YA..`l_pk1!fv_joM+GNqPaFB+Y:6?%/6W[0g>x7B\^8c]y^_t,x?:B~l*h\3w 1c3?x{l<D=+U{&+CuZ@ ;svScY^&-}h1[ kGb[=.jG+c&1 T  Td5O ` 'N}5Uw0nqsU7k sq/k3R`ueh"fT)TL'5s7/bAVu:HP+`<?~R](7|]+<vE=J&Uj$[BkAW2H= GYL\x A4YHI? $pH]'%36O?Q_|$TDa1> YL^@u2 }Y/&' FSt\exXI^J\8.# }eKv vnE L%n9!r0WIL*5m m/PHY?2uX.XN=ryY]D+M j5! :!- $9  R  )  $.) Zt 7*5 ),CY gV 05J!9= 9T+K+l"At59*? - ]'*e5 `FVQ1Q#0}? 4v{"2@3&( !82K'%  S\8(/:n\))=$@)_u]5i/-!E[ILIh )T$O>?soPqY25@O,B2zs"B8>W8Of? c Fr*8N1&H)bBa !8J'(sMDx0 )A*&veE/D >''''''''''''''''''''''''L''''''''''#''''<{{{{{{{{<<<<<<<<<<<<<<<{{<{{<{<<<<{<<{<<<<<<<<<<<<<<{<<<<<e<<{<<<<<{<<<<{><o<<*^44BV XZ?Am]:wkh- P$Zy &>u::P,JdU#']p!`h~^*>pFopQpTSSJFdo1N^'_ 1@ {Xe<}ZR~7q{k#p{dDq_Ra/VJiO)0 gzlvF Hr8<a':}DhJ1O%/T8dE+_j^&z|Y",z|scf(X:\j@f70Z~-dL+( )Y+, +H2HHR)+!W:0n 8gZQkkQId:r*JY/?u!i'sh=#O<S;GzV/} <8) LID:*{M<.7K1[S>"?q[uS~WL[cdg:zXFT):Xiied_#zFR(;}!  (|/ oN7u - W5td&XJ1v6V>IO<?Y]]4m$[j"'N0f52[%nTy.S>_Dl m2)OLq}Gcw:"n .k4{%g,B>r 'E YlwYt0C!'7XmFf@A`\'v2GCCCTTGTCGAAAGTTTCCCAAACAGAAAGTTTTCTCCATATGCGAAGTTTTCCACAACCGTCGGTCAAAAATCCGGCCATTATACTGATAACCTTTCGATCCTGGTTTCAAGCCGGTTCCCCCTTGACATTTATGGCGAATTATATGTCACGATCATACTCGTTACGGCATGGTGCGTAGGCAAAATAAGGTCGCCTGTTCCAATAGGCAAGCAATCCAGGAGTCTCCTACACGCTATAGGAGGCTGCAGCATATGAAAGGGAAATCGGAATTCTTTCGATCCGCATCCAATCCAAGCTTTTCGGACCAGTCTCCTGGAGGGCCCTGCTATCCCAGTTTAACAAAATGGGTAGTTACACTTCCGCTAGACATGTTTGGTGAGCTATGCCCCGATGCGGTAACAGTGTTTAGTATTTCGGCTAAATGGTCTGGGAAATTAGTATGAAGCCGTATGACTGCCGGAGAGACGGAGTATTTTTAAGGGGGAAATGAGCTTCTGCCTCTTTCGGTGTGGGGAGATGAAATGTATATCTCTGGCAGAGGGAGCACAGGTTGCGTAATGTAGTCGGTGGTGAATTGTAACACCATATTAGTTTGTCATTACAAGGGTCATTCCGGCGAAGGTTGCGATTTCTTAAGAAGTATATTTCTGGTGTTTGGTTCGCAAAACAAAGAGCACCGGGAATAGGGGTTGAGACCCAAATAGTCTTAAATTGTTTATGCGAATGCAGGTAGGTAGAAGAAAATAGGGTATGTTTACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-21 02:58:53 -04:00AEPt>AEPt>APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1Pt~B1Pt~BCTSBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?x~TDATA ?x~ӲDATA ?x~RDATA ?x~ђDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~8LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISPP1AMnwP1RLhP1WDtiP2AMMP2BA?P2RLz[PBASSPBASPPCONePCONbPDMFTPDMFPPLOC\PLOCVPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  0RUNT :)RUNT "0RUNT :Rate 0GRunN""JbS/N%PSCAN@SMED SMLtSMPLTSPACA_hHSPACPSPACA_hHSVER4.0SVER SVER,,/Scal@Scan@TUBEG1Tmpr<UserphARphCHPphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt>AEPt>APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1Pt~B1Pt~BCTSBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?x~TDATA ?x~ӲDATA ?x~RDATA ?x~ђDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabFVoc FWO_GATCFeat..GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~8LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISPP1AMnwP1RLhP1WDtiP2AMMP2BA?P2RLz[PBASSPBASPPCONePCONbPDMFTPDMFPPLOC\PLOCVPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  0RUNT :)RUNT "0RUNT :Rate 0GRunN""JbS/N%PSCAN@SMED SMLtSMPLTSPACA_hHSPACPSPACA_hHSVER4.0SVER SVER,,/Scal@Scan@TUBEG1Tmpr<UserphARphCHPphDYbigphQLcphTRphTR&BQ{6Q2\gDT]U'Q>lW+UfyKnY18ds]`?Ne4sX$9WK]}DV&[>:)II;?1EFiRCwh1nk-~4Kb}wLD7X{u./uX8?;pPJwiO*$]_`#?QI}yn%/k%S>p=6"4#OvR#egxy{RB0+T.h^n@2dFllj_odF4N`j`$]O|QK:jin'Dg!~^ZUS+%+M;8)l@.u!n`%Sz*1fCy:*0avFwn&K2fE[p!CODa)x+7E2]Lv3?%diR)6/lsXW}0{vMa`HUX;FEX[Y7!QnS,6dJX!Hiw8XcsG!}PlX87)*i`N-0Xwtej_@q3Luz>.9uoW~!Te4;,}aD[h_R9wQi:A,b?Az{fzVS:3AG}X`AL:2ZE?RVuUvcPkga]FU-`Vn!<|'t*?_:xslBEBuECcO58QiyZ$wpor9Sqwg5j*"gt:>rQ`aE| ?C-~%SPPmlg?)|>QIv5?-Y.pa6:!.tkj^4E2rW.X}=_;!;`sEY5_}4]'Z5P%?p MfaqQleA./q}.}:>JWT|+adJsOlG6]}fWG\|J_KpdFm~;[cMmN2CgGz^n7a!.t;Z\39/hV6XD3Xm?;;DpT73@|ir$1.XHT+O}L6>U{mwY':8 vjgow^;hiksyP:r~64L&eG1H+KB6KP8)s5AVihwYc_`koGhnWe^kQtA+UZ~en?,@-nveo*uhGlB^PY6C}Afz@jXaM~63(|}jGPNYQIxw}H{-*<^k'":?S`,j.uFTc%{M*AqbEdG9}j~:HzpWI23Z;|T92xN:9h[OrJ=M=*b0jj8/FJrlM;8'i2{js.>`AzOpPWT<;u4@"Vd*dzF4/T(wfT;GAze[6m[X1EDE?\g}_t6L>BZuGZ-p@vddD\4ApZTFS$oak"T'oA_AN;)oX6YMI){mnjH[F.A[s:>ZCCd+4k,Z)lO[vHds<-F3x[R2LxC{IF}oH,k#_'>XA[.VJz$*#sejp~DsK2FtSEH$*YYo%rF(5,fX!Hiwe2{sGiSVqrj/.3~V'z(Z=kT/n@6TdO{7>kOptpuze/WR/X2EtERPV]O8oe])%,8Ea-cZ,f*F8B,}&8)TK7BKv'xPoQ`OY}]66{qT[sEF($}v4"/VAEPt>AEPt>APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtAUDT.B1Pt~B1Pt~BCTSBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?x~TDATA ?x~ӲDATA ?x~RDATA ?x~ђDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabFVoc FWO_GATCFeat..GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~8LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISPP1AMnwP1RLhP1WDtiP2AMMP2BA?P2RLz[PBASSPBASPPCONePCONbPDMFTPDMFPPLOC\PLOCVPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  0RUNT :)RUNT "0RUNT :Rate 0GRunN""JbS/N%PSCAN@SMED SMLtSMPLTSPACA_hHSPACPSPACA_hHSVER4.0SVER SVER,,/Scal@Scan@TUBEG1Tmpr<UserphARphCHPphDYbigphQLcphTRphTR