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(    )   *                 ,22(3<:''76:51/1%;'  n,,,III^^g{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR}@RR KKKiii      )))222;;;CCCLLKTSS[[[bbbjjlqqwww}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 3815229f8c724cddafeddc7cc49cf19bSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-21_01-11_06293130Seq16644134536_134536mfagan96-Well*K1313_C9_Tradescantia_zebrina_matK-1RKIM-fKB_3730_POP7_BDTv3.mobSeqPop7zJ- ]  1 ^ f ZIomcq#lZSK+FeDg- ?/?wt48"y0   R  j ; 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'372&  -43) 8N\^UC.  %9FF9# )NkvjL( '/* 0YzwU, #)# "/64*      %&! ).* #./( %/2.#,DTVI2  '.+  -4/"%?U_ZF,%174* .<B<-  "*,&  %%      ,5986541+2M_bWD2($%$  *?LJ<'    2JWTB)  ,;B>0 2@C:)  ,TurM E|j8 #($  %'  &)"  -:=5% -77, $&!  !:Q^\M6  &/1,  !%"   ,ANNA,       9LTM:#     @oo@  ## >\mlY: +8;3" 5diP@YKA\ANAKB.bcpKB_3730_POP7_BDTv3.mobtermGGGCCCTTAAATCTCCCCTGTTTAAGAAGCATCTTGATACCAATGGTAGCAAGGAAGCAAAACGGGGAGGAGTAAAGGGGGAAACTAATGAATCGGACACGAAAGATGATGTCATGCTTTAGAGTAGATCTAGCTTTGCCTATCTACTGGGATTTGGTGACAAATGTCCTTTATCTTGTTTGCGAACTTAAACGTCTTCATGCTGCAATTCACGAGTTTGTTTTCGCTGCCCATTGTCCTCACACCGAATGGAGTTCCCGTTCGTGGCAGGGTTGGGCCCTTAAATCTCCCCTGTTTAAGAAGCATCTTGATACCAATGGTAGCAAGGAAGCAAAACGGGGAGGAGTAAAGGGGGAAACTAATGAATCGGACACGAAAGATGATGTCATGCTTTAGAGTAGATCTAGCTTTGCCTATCTACTGGGATTTGGTGACAAATGTCCTTTATCTTGTTTGCGAACTTAAACGTCTTCATGCTGCAATTCACGAGTTTGTTTTCGCTGCCCATTGTCCTCACACCGAATGGAGTTCCCGTTCGTGGCAGGGTT)6CKW^is| &/9GU`kw!/8CNXhu~ !+8EL^lw~ !)4?IVit$3:HP^gw"-8HUcmu(8KXix !,9BMYdo{   $ / : F M Z g s   & 1 = H S ` j }    * 4 ? N [ k t   ! 4 ? O ] g o }   )6CKW^is| &/9GU`kw!/8CNXhu~ !+8EL^lw~ !)4?IVit$3:HP^gw"-8HUcmu(8KXix !,9BMYdo{   $ / : F M Z g s   & 1 = H S ` j }    * 4 ? N [ k t   ! 4 ? O ] g o }                                                                                                                                 AP.'E0R8$1? 9<6J]_%```Y!F^+]>b/aN$:,AN3'-5#[C"K6Ea^ I,PCW2N^=8!R2*Y#W[1D^.K6!:N$(d&`SF >Z:a0FO:XC!.R_+25#=#bU3\*4#&!.[YON61,B!=1]ZL5 +RR,&+7![/Z-+SZ4Yb=+T(\@UB-P75\ZcYO]5;"P5aXR_W>%DJ\'bV%$!LE[8*'<ON&0N:&KBC6  - MN 7c( (4(CIZF PQCD|7:tl1 ov5v4nzer4 c5z9wr))h1_Seb*.#2_f*sfA0G<%O~Zbjz'3]Xj(@3`sBD`9!owG?d?/Q/^A}-GpRS;?mpmFC)/K~+ *ls55s@N0IZ ( jC$UbC l'OT=~x)SxdwTE/19/Y$9.ie  (;'PT%-%oX0 E  mZ M|i,9Ncw.op&; jZt ,l[ h][n,D&1mvpyNW!yz uZuqdv{Z?|T8P8 A llagL,&?j$3=lA }1s2dO7t pjq%JtJUBZ #Y^[u5Yw&5vuV-is [(EkO-S^}zJWv$f/hiL+(. pVfq ;>_Vr| ;5n!q=m.UM:{~:]qZf%ZH\0mJ( U; WNzO!n8.>sR;R\ets7wpuTCAGGACCCCTGTCTTTGATGGCCTAGGTTCCGCATTGGTTTTGTTGCTGGGCTTTATTCGGGCCCTGATGAGGGCCCCATGGGAAGCCACCAGCCTAGGTCGTTTCTTGTCTTCTAAAAGAGAGTACATGTTTACCCTTATCACTTGTTCCACACTACGATTTGACTACCATATGGTCCATTTCCAAAGCCGTCAACTCGCGCAGTTGAATTCCTGGGTCCGCGCGCCTTTTGGTCAACAGGCGTACGACCGCCCAGTTGCTCCTCGGATAAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-21 03:00:17 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtcASPtcB1PtbB1PtbBCTSBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA 0`TDATA 0`DATA 0`DATA 0`tDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANEKLAST~~wLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP1AM$P1RL$ݱP1WD$P2AM$AP2BAeP2RL$PBAS3PBAS!PCONܟPCONۍPDMFTPDMFPLOC$iPLOC$EPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  0RUNT :)RUNT "0RUNT :Rate 0GRunN""JbS/N%SCAN@SMED SMLtSMPL++TSPACMSPACSPACMSVER4.0SVER SVER,,/Scal@Scan@TUBEC9Tmpr<UserphARphCHphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtcASPtcB1PtbB1PtbBCTSBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA 0`TDATA 0`DATA 0`DATA 0`tDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabZFVocIFWO_GATCFeat..GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANEKLAST~~wLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP1AM$P1RL$ݱP1WD$P2AM$AP2BAeP2RL$PBAS3PBAS!PCONܟPCONۍPDMFTPDMFPLOC$iPLOC$EPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  0RUNT :)RUNT "0RUNT :Rate 0GRunN""JbS/N%SCAN@SMED SMLtSMPL++TSPACMSPACSPACMSVER4.0SVER SVER,,/Scal@Scan@TUBEC9Tmpr<UserphARphCHphDYbigphQLcphTRphTR]nd3ghlc T-* +Wt3z{0bInTor@tbfD7E)0Ie/AK8N'nsv&'Y?sxWlZN.YRz!#fn2%FT!sz^2h3)-I5d~)Uk}`:r&xvr_WG\|8 Xo2h{/S9~i,EAx8amb#QJU0iHAj}W2yv!a!<}B]rQHfKbVU}Uz1p=|(LZjp7xMO9j;q6TB(2JLvuUlN1@#vQ!TMF4FprjDp*K0.]+0Oj!Q4aK{WlVO:}mldKV9 b.nhb{\ByX.]t5+y|`}__';_/A@(#jFnjUZ'KvNIGQ5ENI@[wQi^*w% enqb[*jG3T%ycX}%z/q1wv;{?kJ:hAqTFU-vbNhsrU5-uvCbbJ_<$AJu"#phI*dlSAuR6uk}H4A@hB^4D0e|NY*R2wt\CH8@BpCmhKTI#daC.?bFW&a:OcPX*F8BjhsDgn_RJ4ir$GE1Xz.?e Ml1}ssb[l"G>=?]p&k6v)ukO"yA&d}8qQgE5]U'j*-[~!Tq*nEg8Z~KI'_26?^V$Yeaejp~{>ZHm? >#veu]@|Z5hf6]X?K90#qN8>o XS_xP=O_l;mq;;9VgO(_y"`k:!.tkSY4E2 (# +6~j"zpnIy:]GG3_!./Kr)YW [xinJil]ZCd]'DZNu@v0kc "V&S$~=}F)h>;-T)RJTZmU)P? sIe]b0j[de7#=hM>Wn$sNxoarLE@f2kdZU/0m]ECGKXW .C5wz^`ZdD\4ApZTFS$oa$Fl@i2go~WR&^.|bEdG;_{8@hh/WZ#3|5O-U[dUDNxGq@p'Ic62v^&Gd\{diO}L6>U{mwYBTW .Nio8-I1]f\+P4avFwn&K2f_bA[]/|;vn5mr+8/Yb~;H7.N{CG2CQ>b{`$dbG_?lE8$kabCMV0|>kij7!}WQ`Q&]t[XS:.\_'[wA#Q9Tc T-*<^m9,NzVDSBrRL8~f)NKU|t2#