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')-=+))'   '                                     (    &                                        #$%&'()*+,-./0123456789:;<=>?@ABCDEFGHIJKLRSTUVWXYZ[\]^_`abc#$%&'()*+,-./0123456789:;<=>?@ABCDEFGHIJKLRSTUVWXYZ[\]^_`abcE,,,III^^^{{{&&&99DDDNXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR2079;;;;:99875320/-+('$#  ]]]SShvv   """...999DDCNNNYXXbbhkktuu}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<;;<;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 8579cdef317e416ba55c8cf407964978Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-06-04_18-54_08173130Seq1255412554_Steier_K1340_matk_Rjmadera96-Well K1340_C11_RKB_3730_POP7_BDTv3.mobSeqPop7zA@><::;=>=94/--/244432110-)%##&)-1233456641-)%"!"%(+-..--,,,+)'%$###$%%$#!!%),./.-,*'#$'))('%%&'(*+-./.+'# !&+0343211234467898520/048<@BCEFGGFDCDFJORTTSRRTVXWUQNNR[emqpkc][^emtz|~"FkG,[~f{ 6 m7[ m !A!Q! y.kv#4L { 3mDP)\TmJh F  7s ' 1\NG9 8 Ed 2Q45 (V%NwG/bi&p]Z4    #7Og~q^L</'#$&)**)&#  *=Y{}aK=9;CO_rnT;%  $#   $'('%"  #')++*)'$!    $+3;BEFDBA@?><83/+($!&+./.,+++-..,)%  #&('$ !$$"  %-24310//0148<@DEC>70)"      "##!       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A @lA"hAL JAbKB.bcpKB_3730_POP7_BDTv3.mobtermCATTAAAATTGCGGTTCTGTCGCATCGATCTTATAAAAGATGGGGGGGGGTTCTGGCTTCCGCCATCCGTCCATAATCGATCGGAGAACCCGCCCGGACGCTTTACTTTGGAATGCCTCTGCTTTACAAAATCCTTTTGCCAGATCCATTGAGGAAAATTGGAACTAGGTCCGGTTCTTTTTAGCATATTTTAAAGGGATCGACCCTCTGGCTTTCACCCGGGTGGATCGCTCTTTTGAACAACCCAAGGGGGGAGGAGTGGGATTTGGGTTTATTGCTTTGGGGGAGGCCTAATCACGTGATGCGTAGTTCCGACGCATTTATCCCCTTTTTATTGACAGAGAATGAAGAACCAGGGGGTATCCAGCATCAAAGCTGGGGACACACCACCAGACCACGAACCTCGTGGTGAATGTTAAGAGTCATTTGTTGCCAGGAAGGTATCATCGGCAAACGAAAAAGTTTTATAGTGAAGATAGTACAAGACCAGGAATCAACCCATCATTAAAATTGCGGTTCTGTCGCATCGATCTTATAAAAGATGGGGGGGGGTTCTGGCTTCCGCCATCCGTCCATAATCGATCGGAGAACCCGCCCGGACGCTTTACTTTGGAATGCCTCTGCTTTACAAAATCCTTTTGCCAGATCCATTGAGGAAAATTGGAACTAGGTCCGGTTCTTTTTAGCATATTTTAAAGGGATCGACCCTCTGGCTTTCACCCGGGTGGATCGCTCTTTTGAACAACCCAAGGGGGGAGGAGTGGGATTTGGGTTTATTGCTTTGGGGGAGGCCTAATCACGTGATGCGTAGTTCCGACGCATTTATCCCCTTTTTATTGACAGAGAATGAAGAACCAGGGGGTATCCAGCATCAAAGCTGGGGACACACCACCAGACCACGAACCTCGTGGTGAATGTTAAGAGTCATTTGTTGCCAGGAAGGTATCATCGGCAAACGAAAAAGTTTTATAGTGAAGATAGTACAAGACCAGGAATCAACCCAT(4DNYmy)>P[dlw 6Q^hx $,<Sbm}%/:DM[l{ !,7AM\pz(8FThq!6KXalv ,7ES`iu   ' 3 < G Q ] g r }   ! 0 < M a n {   * < E P \ n z   , @ K U d q y  ( 6 B L W g p 1AO\ix $5@P]ju0;FR^iv"-7EWix +7BP[cr|,:GUgs&/<M[iv'4AM[jt.;IR]iy&8DT_jy)6@N_jw(4CM\kw (5(4DNYmy)>P[dlw 6Q^hx $,<Sbm}%/:DM[l{ !,7AM\pz(8FThq!6KXalv ,7ES`iu   ' 3 < G Q ] g r }   ! 0 < M a n {   * < E P \ n z   , @ K U d q y  ( 6 B L W g p 1AO\ix $5@P]ju0;FR^iv"-7EWix +7BP[cr|,:GUgs&/<M[iv'4AM[jt.;IR]iy&8DT_jy)6@N_jw(4CM\kw (5              "    "3"#                                                                                                      "    "3"#                                                                                        [;(MLZL0F /.NAP0a&SIICaD`CAaI0O "I"a<_9BI 3 "G*K6]G S"BDX!0>-?7.,6RP!!)>.,-JJU[?'b;A3OI#a/^.4?NS@GKE;L@1%aWK(M3:^@)  @JE0+H20?EZ\O]# &=I;-/AGF/W\<!15=MYW3FX2'AGXCH/3 2 [L6@LXHK(O>W':[bKJ1:L? B$K023XQP,]S O 6D<^T+PD5.7 _*V3.'_C-CNV@JQT>>43H-#/T]&8%^0N_[a-D D`1?E(T8D T5,1I%DBY7TID >H3Q+N)1 7#6MX,[M!\SI_cKX .,bL8#>2O%<9/AJP0Y X[!4FB-KN!6 , 1J/NaG`MN- L<:<R=/8;Zb@ G7\CN,M)JKLL("N e3C<3z^R7*6?I"Z  ;d~!ri?@\\u+sJLKdO!lQC4:! pJ.Q ts1!^c:3B5Qi'FA6eD%`c") zZ3DM GLx\w2*/?_ #x<uA*FS| tbfV =J[b6HNfKpmR:% yAXKD_mC!w!`YL$ce g-L2t:hV -Yg'=zER s#qC v7T. g0dh)] QyI%TM%=|& "YDD[mP# 7 z/E]7y|4*{_: wMx g ( ' C6Wi).\ktSO@RE"4#JQNt(B":  &"=K/H_@ew I3 +f{NR`7 2** E$0O K80/ TY=;=93,G" ^7   / %2QL 6X3 c&M6; QY9Y8,7p}E~p7n"hC} 7 =906YV:gp{1L8 S*j \I)^zwK~^q\} 0]9$V\T+m(HhaM#2+i[1&d :vdQ-E!P6 0fe\Eu0~LVm1#VNgnUf"^v)`?iek#4IY#RdkN>d=~wj(vsttYn_hW3 $A;r!AH~}%\H~,uD!4Sz#[mP^V,!iFR56CioC! W q{ 7E~^MZ0oLD5C+On.Rij!f4|/w8r4 hrmy~gU   mm^+qRB*Ab4ehA6(]yD#;A> !* #BSssSQ+7z'wYBD$8|\ /d3H*h3\m!eOw i}l]58KF ]'MZFQXS.$ zoNmx{P-}Nz: &w  gUSN@~~Ogt7QAIW6|'cW2"h@^];g=b*Q^8^o;'rrD%hOI}A8GTAATTTTGGTGATGGGAAAAAATATATAAGATATCTCTGGATTAAAAAAGCTCCCGAAAGCAAGATGTCTATATTCAATCTCAGTGTTGATTGAAACGCGCACCTAAGAAGGGATTAGATTGACCAGGTTGTGGCAACAAGACCATGAGAGAAGTTTGGAACTTGTCTATACTGCTCACCCTAGTACCAAAGCTTAATCTTTAGTCTACTGCCCGCTAGAAAACACAGAAGACCGGTTCATTATATGACCTAAGAACTGTCAGAAGTTTGAATGGTGGCCCTCTTGCAGAGCCAACTAAATCTTCGCAAGGACGGATTACCTCTTTTAAGACTCCTTGGCGTGTCCTTAGCTTTCAATTCTGTGTTTTCTTTTTGGAAAAGGTGCAGGAACAGTAGGCCCAAAGTCCTAAGCCTAACTAGCATGGCCTGGTTGTATTCCAATCACATACGCTCGCGTGGTCACAACGTTATGTATAGTGCGTTATAAGAAGGCGTCTATCGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-06-04 20:25:37 -04:00AEPt3AEPt3APFN hAPXV2APrN /APrV1APrX}}8ARTNASPFASPtxASPtxB1PtwB1PtwBCTS)BufT!BCTIDCTNMCTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!B>DATA!BDATA!BDATA!BDATA #GqDATA #G-DATA #GtDATA #GDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT rEVNT ~EVNTÊEVNTÝFWO_GATCGTyp!!üHCFGCEHCFGHCFGHCFG==InScInVtLANE[LAST~~(&LIMS!!LNTD2LsrPaMCHNMODF &MODL3730NAVGNLNE`NOISOfSc<NP1AMP1RLP1WDlP2AM"DP2BA&0P2RLXPBASPBASPCONPCONPDMFQPDMFPLOC PLOCPSZE`PTYPPXLBRGNmRMXV1RMdN յRMdV1RMdXվRPrNݙRPrV1RUND RUND RUND RUND RUNT 6RUNT 7RUNT  1RUNT Rate ðRunN""ݩS/N%SCAN3SMED SMLtSMPL ESPACMSPACSPACMSVER4.0SVER )SVER,,FSatd<^Scal@Scan3TUBEC11Tmpr<UserphARphCHphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN hAPXV2APrN /APrV1APrX}}8ARTNASPFASPtxASPtxB1PtwB1PtwBCTS)BufT!BCTIDCTNMCTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!B>DATA!BDATA!BDATA!BDATA #GqDATA #G-DATA #GtDATA #GDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT rEVNT ~EVNTÊEVNTÝFTab8 FVoc7FWO_GATCFeat..7GTyp!!üHCFGCEHCFGHCFGHCFG==InScInVtLANE[LAST~~(&LIMS!!LNTD2LsrPaMCHNMODF &MODL3730NAVGNLNE`NOISOfSc<NP1AMP1RLP1WDlP2AM"DP2BA&0P2RLXPBASPBASPCONPCONPDMFQPDMFPLOC PLOCPSZE`PTYPPXLBRGNmRMXV1RMdN յRMdV1RMdXվRPrNݙRPrV1RUND RUND RUND RUND RUNT 6RUNT 7RUNT  1RUNT Rate ðRunN""ݩS/N%SCAN3SMED SMLtSMPL ESPACMSPACSPACMSVER4.0SVER )SVER,,FSatd<^Scal@Scan3TUBEC11Tmpr<UserphARphCHphDYbigphQLcphTRphTRMr,c],~u:L< zFefcXM7WX}lna] 5EX6R2? Cq4GPQFG&908]/eJ!`WBV*O.:U q<6(mu y-WPv8UUCEDFi3V[,A4#OvRio5z{,'~E{}P.Ea}_eaejp~0,{VBtn&gqr;zuhGlB5-S6C,nJ3qo8Mew.KzQ&:C=oq#g%rl(Lii$sL9,X/5`w=yBimJEIoM/V,<|ag&<{[;NR7Q@(f\f6!h7nL%v@g=?k)1mVeP *" }O^:gol%`MvO0Tl_U5TYe0tpJ_~7L3"6%4y^1Y03@a7&^LoT.iP$^=5NDzU46LD&YHE -kb+N4+0|k 1{sIRD8z{&]t[Xd:< dG#$w17h+eJ/n=Y?iQ7wDUfS*rR_V?7Zb0I.9?D%QlmdT.+6P-2ZE<}qjM>Pz;+hj3jT$3o%+0|5Q1V?yRl<`QV-AMV^@c O*Pv4QX`J!hh1 ~i|]-@[3>>B~:^gWT<;u4#kBE=+Hqd-wvXfT;KBTAq;ah^.p2kSGKI'_2q@,p;YCWeq }X'KtFW.: wT_eD4<0|fP%4X+*flVS:3AGXSXW .C5wz^`]vWs*D_rj|YnG|urG&/o{o;Mj2Q&?R2gcV]j4 VIW&VS)TKQoB+BhwD~ '(CwM@B8{A)PS_W .Nio8m*1fCy]_^avep"iK]0T;7[3VT%7;H{Y+]B7b~;HnkKuaXj`Q Dx6m}>7:I12 d+[Y5xo|[Ez1{zK90l_v]M;~6bI.J:*yEwiOOd&79?Ou4[Y{zsyN}@*ohj4TIx*&`_M%Y_bO?C!.tk,8EE.Om,NeLVWYHjD(y$9eKobs>6I(C.FbZ?`gAEPt3AEPt3APFN hAPXV2APrN /APrV1APrX}}8ARTNASPFASPtxASPtxAUDTffGB1PtwB1PtwBCTS)BufT!BCTIDCTNMCTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!B>DATA!BDATA!BDATA!BDATA #GqDATA #G-DATA #GtDATA #GDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT rEVNT ~EVNTÊEVNTÝFTab8 FVoc7FWO_GATCFeat..7GTyp!!üHCFGCEHCFGHCFGHCFG==InScInVtLANE[LAST~~(&LIMS!!LNTD2LsrPaMCHNMODF &MODL3730NAVGNLNE`NOISOfSc<NP1AMP1RLP1WDlP2AM"DP2BA&0P2RLXPBASPBASPCONPCONPDMFQPDMFPLOC PLOCPSZE`PTYPPXLBRGNmRMXV1RMdN յRMdV1RMdXվRPrNݙRPrV1RUND RUND RUND RUND RUNT 6RUNT 7RUNT  1RUNT Rate ðRunN""ݩS/N%SCAN3SMED SMLtSMPL ESPACMSPACSPACMSVER4.0SVER )SVER,,FSatd<^Scal@Scan3TUBEC11Tmpr<UserphARphCHphDYbigphQLcphTRphTR