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E,,,III^^^{{{&&&99DDDNXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR2079;;;;:99875320/-+('$#  ]]]SShvv   """...999DDCNNNYXXbbhkktuu}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<;;<;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: f7c9bd00f6294f8bb38f28db06930de1Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-06-04_18-54_08173130Seq1255412554_Steier_K1340_matk_Rjmadera96-Well K1340_A9_RKB_3730_POP7_BDTv3.mobSeqPop7z5r'f2<w   R$  d  % X " $   6 g P 6 o w 1 F +  ^ (o&5B'-|7n & g e Q'8NLZ", Ie ? c _ `LYt    P 6rC/U2c   bxP  hm)"bMU`wcX5tJE NDt+o0"KYG 7 { AGP~,_(PA&x4(/L ?""-@9GVndq z8~}w)k]3M%=Y/"- 7OX z z G}E)I`;r9!(2C=KZjwzugVBD4L% SS ,11-& R ~85A>5+&%+:V LtsE 8?npJ'A-`k&T^E M % c *N;$Gy:!#.1'@hDixnS;7Sud! |$BC*yW;%/Y[/:KI5%Y~IX:$g"D T+YhX5 R\9$13',[s[WnbXwqeP7&'>dzL&Ny);0|NNY9]Yj{~lK' 3d:@0kD8WiiW:  $>QVK4<[qxo\G826?L_z Bi|zgM5+2Lr)?Tcg_K2 AYT%\/B=sA)4 ""! #" >UOIoB D)D\jhX=   $8A;.)>tHS2}fh{*;/WL`f<#G}&<D-}K,!&1:8+&W^/ /]q{;C } &R ,, ZDYdht0lE<ba#\sKNV! 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"  %$ %$  ! 1FRO?( dFE@ATAG[A)_KB.bcpKB_3730_POP7_BDTv3.mobtermAACGAGGGAAAAGGTGAGGTATATACGAGATGTAACGGCTGTAGAGGGTAGGAGTTTCTTGCATAGACACCCCGGCCGTCACCCCTAAAAAAAACCGAATCGGCAACCCGGCCGGAGGGAACGGGAAGCGAGGCACGTGAGGAATGTCAATTTCGCTAGGGACCCGAAGAAGAGGGGGGGATGAGGTAACGTCCCCCAAGTAGGCAATTGGATAAAGTGGATTTTCTATTGTGGCTCGGACCCCTGACAACGAGGGAAAAGGTGAGGTATATACGAGATGTAACGGCTGTAGAGGGTAGGAGTTTCTTGCATAGACACCCCGGCCGTCACCCCTAAAAAAAACCGAATCGGCAACCCGGCCGGAGGGAACGGGAAGCGAGGCACGTGAGGAATGTCAATTTCGCTAGGGACCCGAAGAAGAGGGGGGGATGAGGTAACGTCCCCCAAGTAGGCAATTGGATAAAGTGGATTTTCTATTGTGGCTCGGACCCCTGAC(1<GPVhr{!-6CKR]hv !)1?JR\ix",;FWdp},6?KVds *8DR^ht'1;JZcp| );ESbn{ .8EP[gu    ! , 7 B N W g r    & 1 > O ^ j u  !(1<GPVhr{!-6CKR]hv !)1?JR\ix",;FWdp},6?KVds *8DR^ht'1;JZcp| );ESbn{ .8EP[gu    ! , 7 B N W g r    & 1 > O ^ j u  !      %                     "  "                             %                     "  "                         PH2^Y: & , VM^:0Z$\QA9bbNJPa6_AY41 N ] `[LS _%Ba]AX U\S#(Q5bY0X aEVdP 1W1Gc^PRN& ZF3( ;&_/b* T EXV_c!, a^Y Rcb CL7AY- 1!&YOXJ_Z^$Y%8UG,U#&JG8Z8%W29+CcN%!E8&J `)H*B#2a[2%;.RGU82c . = < " r| Gb  B+ 4  8" #>eu$mxt!Qq6C-eVV:=7 Yo&tm 8OK+Fa.= 6bY bA)VUC? nV| 3u_1wm Q8kf L%Z-i & Hs0c v<*VO -o8l9,t L&[NJKv Y9 (4oC 46OOPC+a[#m5qR/f[XjR|Xk^Z{35cF(Z$s$C#>" 5Y R !D.(,pMysZ%&p1 psp~3ppppuU^W7x,ook1.19!(Mp0gcv FV$0~%.r-,8X#R KC*zLX~,?,pgd3h  | j h  ,     u <|9JtI e UX4Q MEi;z>T)LBzHkq>js}pVdUk|jCk@@ud=`dA]h 4sNSRWd6gmH:CCAAGAAAGGGGAAGATAAAGGGATGTGAGATAGGTATGGAGGAGTAAGGAAGAAACAGGTTTCGCCACATAAACGGAGTCTTTTATTTGGGGGGTCGTAAATAGCGTGACAGCTCCACCCTCTAGTAGCTTATCTCATTAATTACAGTCCCCACACCTTCCTAGACCCGGCGAAAACAAGAAGTAGCTTTCTTTTAGGAGGACTTTAACCTACGGAGTATGCGCTGTAGTAACTCGTACGGAGGAGGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-06-04 20:25:25 -04:00AEPt3AEPt3APFN APXV2APrN OAPrV1APrX}}XARTNASPFASPtASPtB1PtB1PtBCTSBufT!B#CTIDCTNMCTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!B^DATA!BDATA!BDATA!B$DATA #:FtDATA #:Ft+DATA #:FtqxDATA #:FtDCHTDSamDySN Dye#DyeNDyeN DyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEOLAST~~LIMS!!%LNTD2LsrPaMCHNMODF FMODL3730NAVGNLNE`NOIShP1AMKP1RL[P1WD ;P2AMP2BA P2RL +PBASPBASPCONcPCONkPDMFpPDMFPLOC{PLOCPSZE`PTYPPXLBRGNmRMXV1RMdN RMdV1RMdXRPrN۹RPrV1RUND RUND RUND RUND RUNT 6RUNT 7RUNT  1RUNT Rate RunN""S/N%`SCAN3SMED SMLtSMPL eSPACMSPACxSPACMSVER4.0SVER SVER,,fScal@Scan3TUBEA9Tmpr<UserphARphCHphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN OAPrV1APrX}}XARTNASPFASPtASPtB1PtB1PtBCTSBufT!B#CTIDCTNMCTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!B^DATA!BDATA!BDATA!B$DATA #:FtDATA #:Ft+DATA #:FtqxDATA #:FtDCHTDSamDySN Dye#DyeNDyeN DyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab FVoc FWO_GATCFeat.. GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEOLAST~~LIMS!!%LNTD2LsrPaMCHNMODF FMODL3730NAVGNLNE`NOIShP1AMKP1RL[P1WD ;P2AMP2BA P2RL +PBASPBASPCONcPCONkPDMFpPDMFPLOC{PLOCPSZE`PTYPPXLBRGNmRMXV1RMdN RMdV1RMdXRPrN۹RPrV1RUND RUND RUND RUND RUNT 6RUNT 7RUNT  1RUNT Rate RunN""S/N%`SCAN3SMED SMLtSMPL eSPACMSPACxSPACMSVER4.0SVER SVER,,fScal@Scan3TUBEA9Tmpr<UserphARphCHphDYbigphQLcphTRphTR-Dq.$+W x:H-p9bgh`U*lO6viO$@I{-!`:ZPV2fI&$yNihB:Drj{H,:MX]#=c$DfqcdsTF]luKgk]35FQ6nB!X`7.w>6?xHf.QR9JK[YlyjSu30&reA .3zqg94$4oAM%/^=f:hN{+eqL}4|Md'ac_`k(U.*C]J=E]1|eKW3CNIG2gbXW,VE+0&'7%4X+*fl979v$sp3@=?]p&q!R;{?kJ:h+p?H(+Q;T`h3/[ze/G,y"o-,(B~b) e:=8}R"wKgpjm2D%~4r8h98&>LbsMvF(wD1RuM]\K+dIo&!i_&,? W]H6X+9mvH%lFU?-4$&FkNp&iJ-[qr[&Xj|82Z~9}j~:HzAb9ECQ/}Z&['K2;+hj3ST?0sKG'BNp3JWTX+9_e VO[O~}4}XfSap<#2_<2R57}?hik?]wJUo6X?/WZ#&a:Oc!X|eDNJ{s3x>rb TQ3U'.QR0Iel1}ssb[l"G>=?]p&qqQIxw}H{-*zir8rP p'Ic62nFT16Y=*"KzM ZmnHN{$VyJIit5+y|`}__';_/A@(#jFnjUZ'KvNIGQ5ENI@[wQi^*w% enqb[*jG3T%ycX}%z/q1wv;{?kJ:hAqTFU-+%3N;KBYgn8&AzfG&nak'eaK|`h3/[zeqr[&Xj|82Z~9}__';_/e"^=0`VZI;P>W+YYo%+0|5Q1V?ORJedjY{mwYBTU;CxhKn6[MHSVi220b_'{aCA{%?_CjFDhECel7B]%jxxsWDy4<|O{^Mm[*&`_@-|T+a6oGhnWe^kQtA+UZ~en?,}&Dg8YauBQBhwD~EG7&o.jax]CR"lWW .C&qN^Fu*tw9E^u4[Jn\9-s1tog,s48tNhA&!qH$AEPt3AEPt3APFN APXV2APrN OAPrV1APrX}}XARTNASPFASPtASPtAUDTdd.B1PtB1PtBCTSBufT!B#CTIDCTNMCTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!B^DATA!BDATA!BDATA!B$DATA #:FtDATA #:Ft+DATA #:FtqxDATA #:FtDCHTDSamDySN Dye#DyeNDyeN DyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab FVoc FWO_GATCFeat.. GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEOLAST~~LIMS!!%LNTD2LsrPaMCHNMODF FMODL3730NAVGNLNE`NOIShP1AMKP1RL[P1WD ;P2AMP2BA P2RL +PBASPBASPCONcPCONkPDMFpPDMFPLOC{PLOCPSZE`PTYPPXLBRGNmRMXV1RMdN RMdV1RMdXRPrN۹RPrV1RUND RUND RUND RUND RUNT 6RUNT 7RUNT  1RUNT Rate RunN""S/N%`SCAN3SMED SMLtSMPL eSPACMSPACxSPACMSVER4.0SVER SVER,,fScal@Scan3TUBEA9Tmpr<UserphARphCHphDYbigphQLcphTRphTR