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"  '$ ! 3&4(-, & & /  -    - ""5&    !!  !                 #,              "   0 #   #)   "1#       "  !                             & %"*+/5,1?8,29=9<8ZE9;0J51LFVm[WzjmqjahN_ZDeMoNpUNaXUv@]VSMcPKq^mZ@T9U<L7.<>+1'',%(!!+  ~z~zv}}r}         ER- nH             5#"& /HKKp*r.% S *{ sx- !{]@X+1R[ /BR2/ &SYz)@M(:0|}wD.y X>9) sxkchy+rZJ=IF:-3*6VE3>;cT2Q&RFJ)2  *++'1F:HAD?LVMIPhWluwptp~wgeMN9/;2<%1/3%8;3NMC?A<CEG<=.%*5,%    --1#$51    ',/4-'9     *# !,$ 0(A17-!%"++,G;0$03&%)         "$  -9DUJmYbmjlhmqalr`pspwvcghHH.+      #+9:%4',(  )-#$    )    &   $&      ##:#' "0%,%!                                                                                                                                                     %                                                 E,,,III^^^{{{&&&99DDDNXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR2079;;;;:99875320/-+('$#  ]]]SShvv   """...999DDCNNNYXXbbhkktuu}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<;;<;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 29b8c034422d48a7a21b1735eaf9355bSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-06-04_18-54_08173130Seq1255412554_Steier_K1340_matk_Rjmadera96-Well K1340_A4_RKB_3730_POP7_BDTv3.mobSeqPop7zwoiden}l[e)(6=?<7." >D-~~pnvwuzsd]dwn`e} 4Rgqoiegp -Mk }4 % #_)./M5k}qkv|zttmf_XQzIA91*#CrxK K!E$_(*,1&5:=@'AAi?<83.)(#!>.+ 8 D 5 M t P S 6de]br\Rw 9 gCk$"v&(w))r'%D!nxl  b~0>nF8  U(4ANaYb!'C]nrgR8! 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!%  X2K&461H7+7;(+1],GQ6 ZGCQR[F] @Q JC:8CVU/KG:<RUZH'ITa%(%+7US\AS')=7]bF64693#U-<DSD';(L FH1I\ &4 ?<$Sb] N$'Y6,T/;_<UdFLAPy.{A]||(jB6{2B` `K5vV!f a\3\%6VjCz!6;9N"1;:_ /a>'Y'1S2o5\N&> P hNFFwI e Ga?`vvo~{0%Q>[\cktEJ>C?1 MNMB): f[Tk /!%aPO. *01 !9.dA 1R|stiwj8 6rV`#r_}dc"qYw+]l<8Cwje?kHLpR8Rd7,Aw<gpSrgdHX,P8/ Jp{a< pDI8jG8d2"bxp.YL16hZvk)"M{~w+5+vhDR~#~~?;OwibiT@\%|.\G@%0U}fg RE<:dJ*miG6'N9^~oRM^lK<,ND9\rqso!]_ EZS;n<jy\ocATGATGATCANGAGGGTATTTTGAGTAGGGTCTTTTGAGAGGAAACCTCTTTGATGTGAGGCCTCTTTAAATCGGGTACAGGGAGCGACGAGCGTTATAGCTAACACAACGGTTGGTAGTTCATTGGTTCGGCACACTCCAACAACAAGTAATAAAGCAACGCCTGACTCACATAGTAGTCAAATTGGAGTTGAACGCTCCACTAATCTGGCTGAABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-06-04 20:24:36 -04:00AEPt3AEPt3APFN APXV2APrN OAPrV1APrX}}XARTNASPFASPt>ASPt>B1Pt=B1Pt=BCTSBufT!B#CTIDCTNMCTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!B^DATA!BDATA!BDATA!B$DATA #GnDATA #Gn+DATA #GnslDATA #GnDCHTDSamDySN Dye#DyeNDyeN DyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~LIMS!!%LNTD2LsrPaMCHNMODF FMODL3730NAVGNLNE`NOISPP1AM P1RL CP1WD P2AMP2BAP2RLSPBAS[PBASPCONkPCONPDMFpPDMFgPLOCPLOC3PSZE`PTYPPXLBRGNmRMXV1RMdN RMdV1RMdXRPrN۹RPrV1RUND RUND RUND RUND RUNT 6RUNT 7RUNT  1RUNT Rate RunN""S/N%HSCAN3SMED SMLtSMPL eSPACMSPAC`SPACMSVER4.0SVER  SVER,,fScal@Scan3TUBEA4Tmpr<UserphARphCH~phDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN OAPrV1APrX}}XARTNASPFASPt>ASPt>B1Pt=B1Pt=BCTSBufT!B#CTIDCTNMCTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!B^DATA!BDATA!BDATA!B$DATA #GnDATA #Gn+DATA #GnslDATA #GnDCHTDSamDySN Dye#DyeNDyeN DyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab"nFVoc"]FWO_GATCFeat.."/GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~LIMS!!%LNTD2LsrPaMCHNMODF FMODL3730NAVGNLNE`NOISPP1AM P1RL CP1WD P2AMP2BAP2RLSPBAS[PBASPCONkPCONPDMFpPDMFgPLOCPLOC3PSZE`PTYPPXLBRGNmRMXV1RMdN RMdV1RMdXRPrN۹RPrV1RUND RUND RUND RUND RUNT 6RUNT 7RUNT  1RUNT Rate RunN""S/N%HSCAN3SMED SMLtSMPL eSPACMSPAC`SPACMSVER4.0SVER  SVER,,fScal@Scan3TUBEA4Tmpr<UserphARphCH~phDYbigphQLcphTRphTR6}k1$AZE]bZ&[VGgPH/#8|.IQK%=G{}(-TPrbJ32Qy]xwY>2hpu:In8nZZ*W]U_I[Ddg/.9u2jDTWy2>y^q3Th$Y thENWNv3[My I3)yfuq.$>2tp(kA;M4[{nYD3RhW~^N@.]nUHw>VihGUNM4p[K2:eMvdQ0kAr2GTrhV KVSFsA b5}8 v/,J6v`ZeW\j//u@8Q CKOjLH#[,bTmq/*7ag%gcmeLMaCG !9W&a:OcPC]LVn!<|'{XW,@|sF4GG\}d M;Y@iGtX[3>>B%P^gWyR;uIkrE-O)\rS|5qk`T1jdRC WIFM/|RB"H?+Qg.Rd+ol1+a,|&bX*F8Ba+b8EYWC.%oKO> `VsIefaqQl&A6PG>}Oujd+[Y.m/wBN $`?"*J[x5FaGwpBT9\qwH=qd)B*vH%lF1?HOeCvpN^3jNRjnMgx*&`R@){wJUo6X?/WZ#&a:OcP$SXtmx+9Hgn<_swW^$buJrUlM;_}$A>e6bZ( iu2Zv&1`Z=WcDArh]|Ko5qS+SP7T]TcSI*$6ey(B~beaA({]/wQ2a52rW.X}=1m)h>VO@A$^Rc[6R#7Jh=VEgM9I@O5+znJO!p8Npd-(bcnP6GZ]#(lCKOMQw T"S2Mmn[Ca,k +9}WQ@UzbaI23ttrRXc|LVn!<|'kU$3g;Z6Obl% eIe5_@{&]t[XS_Q7u2&q6X+9mvH%lFivFUUsDG+TK#6] TRFM/|gEmpt^14t.N^n"AQcGf(c,sV_#r[KIxLqRJ4ir$GG\}d MfaqQl&A6PG>}O!p8Npdpu#Wc4hAZG|JWIKT+}tr2LxCmqL}4|Mde_'61Qve])%,8=0?})jZ>zir8rP p'Ic62ir$1.XH`HO5QqE`|mwP@rB ]:]HE^OXv&nP_[O~}<"&eG1h3/[zGq;)y6}c8dgp3^|o:Xz zFA\|5DhECel7B]%jxxsWD@xG57'9\47./D?*&qDp-o*Cwh1nk-~4Kb}wLD7X{Mf4-gAEPt3AEPt3APFN APXV2APrN OAPrV1APrX}}XARTNASPFASPt>ASPt>AUDTdd0B1Pt=B1Pt=BCTSBufT!B#CTIDCTNMCTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!B^DATA!BDATA!BDATA!B$DATA #GnDATA #Gn+DATA #GnslDATA #GnDCHTDSamDySN Dye#DyeNDyeN DyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab"nFVoc"]FWO_GATCFeat.."/GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~LIMS!!%LNTD2LsrPaMCHNMODF FMODL3730NAVGNLNE`NOISPP1AM P1RL CP1WD P2AMP2BAP2RLSPBAS[PBASPCONkPCONPDMFpPDMFgPLOCPLOC3PSZE`PTYPPXLBRGNmRMXV1RMdN RMdV1RMdXRPrN۹RPrV1RUND RUND RUND RUND RUNT 6RUNT 7RUNT  1RUNT Rate RunN""S/N%HSCAN3SMED SMLtSMPL eSPACMSPAC`SPACMSVER4.0SVER  SVER,,fScal@Scan3TUBEA4Tmpr<UserphARphCH~phDYbigphQLcphTRphTR