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6 0 . : 9 ) 8 2 * $ 0 ( & ( " . ; 1 8 . K % U 0 . = @ / C 5 - < A 4 ( > G 3 X E E V > D A ? = / 2 . . 9 A ? 3 0 0 , / . = 2 2 @ , ' 3 6 ; 0 + ' . 2 ' ( $ / - 6 D . ; ! % 4 2 2 7 H D : G L C T E V C G L O Z T C B ; L F U K K F > F ? 4 9 " 5 > 0 > L 4 3 A 2 ] C V L I X W f e l i x h w f i { m r W T L S A : 2 - 1 + 1 2 2 - 0 D 8 1 . : , ) ) 8 5 0 A : 8 I ? M E G A @ L 8 K J 6 ? 6 B L I B @ 3 7 + + 2 I 1 * 0 - * 0 < . ; / . 2 # : , 2 ) 8 G O B I b S F N W I [ _ O m p U M M J 8 @ N 3 : 8 B L 8 < 3 P V \ ; N > Z P T R W j R P Y B F d L l ^ [ e ] } m d s o e \ D X D G 4 = 5 & A & - 5 3 ' 8 0 . ) " - 7 8 0 ( 1 , ) 6 - 5 6 + % ) + , ) ' - 3 " / $ * + % P B 1 % 2 @ : B E 3 - ? & < 6 7 8 N A + < 6 9 / < . % ! 7 ) 6 7 - 2 / ; / ! * ( * & 1 & 1 3 1 * 4 - / " + 3 7 - : < A + E < I ; 6 G ; : U K H F L J N . X Q R P \ D H @ 6 W @ ; : 8 ' . 9 ' 7 ; ' 6 / 2 @ > 9 [ _ c ] t h t x z ~ n u u | x c R A E 9 / 9 : F 5 1 ( * 5 0 : ' 5 / 1 . & 6 + # $ ! ( 0 ) # % + 0 4 ? ? / / 8 = < 5 , 9 ? : G H ? S E Z M - B G Q E N ; B I . ; 6 F 4 ? 1 N V \ G W M L M U J W A J S W p \ b _ m n r w l { | ~ p _ R ? O ; E 7 ; A . 5 5 ! : 5 7 , : % 3 # C ( 6 5 ; > F Y G b ] c k t M g d Q Q W - ( @ @ 8 6 1 5 5 F @ ( " * . & ' # 0 , , , . 7 0 1 + 7 ) ) 0 - 1 6 % 7 $ 3 0 7 + . , 1 ) $ 1 - 6 4 5 ( 6 6 2 E ; . 0 E > $ B 0 F Q J N : 3 @ 6 B 5 * 0 2 , < + / - / ) ' - $ 4 ( 0 1 0 ( ; N > 8 ? ? = > 9 ? I ; 4 * F A , 8 . 1 0 ; 1 % 4 ; ? D N @ 5 A < I T P C R T Y K Q : M T Y _ d f Z e \ ] d ] C O N G G Q N T ^ f p l | m h m d u c ~ x T ] C P = P D - = G ? O = 3 ( < 4 > 3 0 , / . , L 6 " * 8 $ < = ; < + : < ; @ 3 $ ? $ : 9 ) + . < 4 / 0 : O * / H @ @ 8 = I L U T > _ N U T ^ V O M [ L L ? G ; T N K 8 9 C Q A 2 9 H 1 1 H < @ . 4 4 $ & " 9 / $ = : 5 > / @ O b V m j l Y m [ Y _ S O Y F E G 7 > 3 > 4 J @ 6 $ 8 8 2 7 . = F 5 ( ? 1 - : . 1 ! . ) 0 3 - 6 C 6 + > G I \ A I 9 J C D G = 8 @ 9 9 ( 5 8 3 ( 7 * / 7 ! : ; E 9 4 * 8 C ; < " - ( ) + ' = 2 9 : * / - ' " * 8 * 2 4 % 3 @ 3 * 9 6 ? D L m _ p i i u h b O M T b R 5 @ T 6 L U U T H [ A I e L ^ ^ V ` L 0 N A J 6 H G C @ N ? 9 ? 0 4 ? , * 8 - 1 7 C G C & * < C F H Z O h p d } r t p j ~ x u u s g g Z K K G E ? E & / B 2 < 2 7 9 ' 8 : . . / 0 2 ? B 5 6 5 7 2 3 9 5 : 1 C C 7 K ? C 1 P > < G Y O G T K A A I D H I * 9 K B < < . # & 3 ; ) / % O . < 3 # ? 6 : P < L E W f f F l m ` \ ` l ] U E r { v y { m ] V J G M < > ' ' 9 $ 2 ) ( 7 # $ 4 , 0 ( 7 > ) / . + 4 4 , , # 4 1 A # 9 4 * 4 8 0 L 4 C ; M Z Y H 5 B B A 0 = 4 ) 1 3 * 6 B : 1 - $ C = 6 / : 2 < 9 N ; V E T C J F ? S ; B > U E ; N I b E E 4 / . = / ) " ; 0 ! 2 1 2 / 5 ; % / $ - ( 5 * $ + 6 - A 1 F F A F X f ] ~ Y u e j b x b ^ S P @ I K T 6 T ' @ 7 / < 9 + 9 . : 5 ) 4 + 5 ) 6 3 0 2 / + : " 7 / - 1 5 7 ' ! : Q < @ = : B A K R R S L B G 9 Q = = 1 < 9 ; * @ 3 0 ' < % - 3 * ' 1 = 6 1 9 B B F M V Q l m U r x ^ d c j \ h Z a a W U Y ; C @ R 5 4 7 2 7 = + * + + ( * 3 & / 4 & ) > ) + # 8 : H F S C c T m d d T c n O U f W Y s \ c c o d Q Q R < S T D = L & 8 7 % ; J 9 : < 8 = 0 J B 4 ; 9 ; E 1 - , > C + , < 0 ( * 9 1 - ) ! C 7 6 A K I 7 9 M A S [ M R N @ S P 8 B B b T _ = I N C K ] y n i l j d d a X W V Y I O Y U N g a m s h r ~ g q { t t s s x k q z y s j y s s u [ i W \ K ` D @ U ; ? 8 1 = T 4 A . 6 9 9 1 , $ # ? - 8 - 2 3 8 < , ' 0 . 2 < ' . 0 8 / < 8 ( ) % ( + $ $ , # = ) * 4 $ ( 7 1 / 8 9 8 F F Q P : 6 L F J 2 G B Q : 1 T A M 6 C @ H D L S B = < M E 6 P L B L F < 4 C : D B E * D 3 < / 5 @ = 2 A ? . F H k a o q d p r b d Q X G 9 G 3 - = 5 ( * % ' 0 ( / / 0 0 2 / 5 5 ) 2 ) 6 3 : A L ^ T J Q z U d r H U R S B B H D L K 3 G 6 0 8 - ' % 8 ; 5 H 0 : < ? 4 . / 3 & = 3 6 1 1 7 9 & 0 5 - D 3 C - 4 5 1 5 # / 3 + & . 9 % 8 5 5 5 ' 4 < 6 = & " * 0 / % 4 * 2 3 6 2 @ I N M D H I N _ u e T m d b Q m t k s b i ^ a b g S m w t T u s f g x m h X = G Q = ! ( + 7 $ * & % 4 ! $ 0 < > @ / ) ; 6 K S W E X a z w ~ ~ x s y | ~ h q b i > < Y ^ < - 1 4 0 7 # : @ 0 $ 5 ! 1 5 C # > I 1 8 & . ' A 3 E 1 B 8 4 = C R . E ; Z I A F 1 > 5 C N 8 @ = D I A / 6 ? ; C < 0 \ W A H 8 E M 8 D J D H I ] U G G I R ` = C L R E Y R a Z a _ m f n ` u b j H W L 7 I L . : ' 4 A 6 9 ; 3 % % 6 # * 0 ' 2 $ 5 H D 3 < I 7 : O L G ; H 2 E A D K B 0 7 5 ; N 7 @ 6 3 B 9 . D N < @ 8 > / Q / 5 0 < 0 ' / 5 ? ( 1 / , 6 8 . + 8 ; 5 G 9 9 H H = M B F K Q = S T R Q A H E A X I X \ z m [ m Z y ~ p n b _ W | _ ] m u Z v c d k d f k j U \ F S @ T N < P X O R I B M R k f i n p { s h a d U k d W Q O U [ ` E < * ( : . " + % , ' - . $ ' . ) ! . # ) + . L Q - 9 5 7 A N < < L 5 : 6 K B Q I 7 F 6 9 @ . 5 + % - 2 ! / % " , ) % ( 1 " 3 / ' - ( Q ; ; : G ] U H Q Q Q c _ U j p o o c c o ~ b s p _ q g o o p i h v t i w w y l | j g | t c o U u W _ q c L P I E C 1 + / 2 ! ' 2 1 + # " & 1 0 ! 0 4 , : . : 1 D > 7 # 4 , > @ / @ . ? # * > . 4 ; D % ' " + ' * / ) . ) 0 4 : , 7 F 9 C ; 4 : $ J Y Z > < D < X ? O g d g U U O W Y Q U ] X @ H M c F I N I I A 3 : 9 S A Y e g \ g L c O m i O \ M = E 4 J C G A [ a c F g y v n c n k q ] a Z Y V [ Z m J W e [ T } c d v l p v y u b X S X [ Y V V P l O b [ e u y v } ~ u l o k S T @ G I 4 5 $ 0 1 / / + 2 / : - 2 ) ? < $ # 7 0 - % / " 1 ) B . 2 4 . F 0 2 1 & . 0 F : 0 - 1 ; < D G O 4 F , B ; > 9 3 @ > + J 8 A P ; X h p v z q p P R H P F 1 2 W ? C ] S P a [ e \ ] S j [ S O @ ` B ? / ' E / / 1 - 8 6 $ ! ) ' > = ) . 3 , 8 3 # / 0 + 1 7 7 - A : ) D B C 6 C Q 2 b T O _ _ v q z g l l q z ~ Y ` j K Z F T M 2 ' ! 1 , , 2 1 6 2 % ' 1 & 2 ) 3 ; , ( . F , + > ( - 3 * C + 9 C 2 . * 8 0 2 2 ! & , + @ 1 % 7 7 Q D F : D F Q V P P = = 7 X W T C N ] J e U V j ` j z p d a x v } X w q n k k n e W [ > M Z D R N U [ = P P C b X R d o { o x u r f d X U h L H K F E J K A G Q @ O L ` K Y x d a r W v u x t ] u Z h d N 6 9 V U J . A % ( = 4 ! A / > 7 K G > = 0 < 3 3 B : 5 A 5 2 1 ? C 1 P 9 H ; U N R L ` Q H V r i ` i U W p Y e g ] z | y z y y \ i Z l 6 N D N 7 H H F + Y < ; 2 C C ? ? L * ? C E D 9 1 H 0 : 9 6 5 B ; : ; S D J Q Q U B M H O ` S \ S < C W > G Y : H N f \ b k v p ] l q n X f i S 3 I X ; 7 < 4 E D # 1 < + ) , * 3 7 0 ) . = C ( / 3 8 ! * 3 ' + : , 2 ' 3 $ ! ( - * + D % / 4 # . 2 6 3 & * ) 8 + ' 9 ( / ( ( / 6 J ' B ' : , 9 1 % - 6 : ! * 0 ? ( , $ B ' < ( % 3 ) 8 F ; 8 9 9 I v W M O [ n _ ` ^ ~ i U [ j T S X T V I Q W _ X ] [ a W K B L 7 I O ; J H F > B A " 4 2 ? 8 * 7 ( / ! E 1 1 " ) ( ' + % ( . 4 / A ' " * = < F ? E = R 7 Y b e f p M L K H M Y O 8 Q D E P V K O N o X _ j E S ^ Z _ h H X Z j ] X M C O R d Y Y Y h Z _ O Y ^ [ H L J Q C O I L 9 < A G ; 3 A ? H L S N R p N c r } w { n h k ] ^ L J ^ Q L W M Q E C > B ; : ) : 4 8 0 7 9 0 3 8 5 , ; 0 A 0 ! - # 1 6 5 ! - ) ' # / & $ ( # ? 8 1 < 0 @ . @ [ F V V l _ j j ^ d o u h Y t [ m c Z c a U a { \ d X \ N F ? @ J 9 D Y T J E X H V \ N T S E g m _ N C [ Y Q j b _ w l h r l | p h | p g } r | q } { } ] d u d o j ` p g w t z s Z k V X Y ` Q Z A @ 7 Q : D 4 > 5 6 ( $ - - 2 . A * 0 ' < ) - + . 7 4 7 C D : L C E ; 7 C H I [ 7 H C J N W O q Y \ U p ^ U U f X O X P G S ? 1 > 8 3 4 + ' . / 3 / " & 5 , * - 1 ( . , + 1 # @ ! = ; 4 . , 9 : / ( 4 2 J O K = L T ; ] P Z I c j ] v g w x l z { } w ^ Z U R E B 0 = I 6 C F B . + 0 1 0 9 2 % * * ' - + ; & * . $ ! ! * 1 ? ' ' ! & 0 , ' # " + " ) % - : - # ) & 3 4 , $ ( 1 * % ! 3 # 0 $ / ' ' , 0 % # < + " # % , , % > ) ( 2 < 0 0 = S D , > K G 4 Z Q I S g d b a P V L p Y X d y ` \ n k q y u } o v j u l ` g e Q T B Y R G H G P T a \ 6 P c L g e i g h w i q | b U d N Z V R F I + 0 @ ? 4 6 0 & 3 ; Q # ? 1 , 5 & B , 4 7 - 4 1 A 2 ; I B F ? . 9 ? 6 ? : ) - % 0 1 2 F 3 ? + " + 3 , ( ! ( + ? , 7 ) ( , $ 1 * . 1 1 - - 3 = 5 1 < 6 6 4 3 . A 5 D S @ : B M R U R F Z = M t U P X e U g m [ U N [ K b ^ Q [ H P H 2 2 7 ? 9 , 8 ( = $ & % 8 6 . L < = B 3 I > I c h ] F D D P J 8 7 0 2 0 E 1 3 @ 5 0 : 2 : ( 0 ? ? % ( ' 1 ( 2 & 3 : C 1 M = Y V M Q Q G f Z C [ [ S W n Z c f U l u ~ h y o | | | v t ^ Y ^ N Q F : ; I . / ; ; 9 # ' + . $ # ! ( ! ) # * ! , - + ' ; $ $ ) , 2 ! 0 7 / & # . 4 ) $ ' ' $ + 4 : 3 * . * & ) " $ ( 1 - / 0 ) . 0 . & ( / . / C # ; J N 6 > a ? H ; = ; D F @ 2 / ) F & I * + 1 ( 9 % , ( + , , + 5 0 ) . 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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 374e6e5c3b1e48c6b8ae01cff1a742dfSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////
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00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-06-05_00-57_0821 3130Seq 12552 12552_Steier_K1339_matk_R jmadera 96-Well K1339_D12_RKB_3730_POP7_BDTv3.mobSeqPop7z < : 8 5 1 . / 4 ; C I L J E > 8 6 8 ? H R X Z X T Q O P R V Y \ ` d i o t z #6I^t >d(Ty'V-] .Y{^<vK `C%xbTMJGB;1#mXG6"
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KB 1.4.1.82018-06-05 02:29:00 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN O APrV 1 APrX } } X ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT ! B # CTID CTNM CTOw CTTL CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ! B ^ DATA ! B DATA ! B DATA ! B $ DATA * U8 DATA
* U8 9 DATA * U8 DATA * U8 9 DCHT DSam DySN Dye# DyeN DyeN
DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE Z LAST ~ ~ ~@ LIMS ! ! % LNTD 2 LsrP a MCHN MODF F MODL 3730 NAVG NLNE ` NOIS 9y P1AM 8 ] P1RL 8 V P1WD 8 d P2AM 8 sl P2BA z P2RL 8 l4 PBAS =H PBAS 9 PCON R PCON OT PDMF q PDMF 9 PLOC 8 H PLOC 8 @ PSZE ` PTYP PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN ۹ RPrV 1 RUND
RUND
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RUNT 9 RUNT RUNT RUNT 9 Rate RunN " " S/N% 9q SCAN 3 SMED
SMLt SMPL e SPAC AZ* SPAC 9 SPAC AZ* SVER 4.0 SVER SVER , , f Scal @ Scan 3 TUBE D12 Tmpr < User phAR phCH 9 phDY big phQL c phTR phTR 1A This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN O APrV 1 APrX } } X ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT ! B # CTID CTNM CTOw CTTL CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ! B ^ DATA ! B DATA ! B DATA ! B $ DATA * U8 DATA
* U8 9 DATA * U8 DATA * U8 9 DCHT DSam DySN Dye# DyeN DyeN
DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab $ FVoc FWO_ GATC Feat / / GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE Z LAST ~ ~ ~@ LIMS ! ! % LNTD 2 LsrP a MCHN MODF F MODL 3730 NAVG NLNE ` NOIS 9y P1AM 8 ] P1RL 8 V P1WD 8 d P2AM 8 sl P2BA z P2RL 8 l4 PBAS =H PBAS 9 PCON R PCON OT PDMF q PDMF 9 PLOC 8 H PLOC 8 @ PSZE ` PTYP PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN ۹ RPrV 1 RUND
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RUND
RUNT 9 RUNT RUNT RUNT 9 Rate RunN " " S/N% 9q SCAN 3 SMED
SMLt SMPL e SPAC AZ* SPAC 9 SPAC AZ* SVER 4.0 SVER SVER , , f Scal @ Scan 3 TUBE D12 Tmpr < User phAR phCH 9 phDY big phQL c phTR phTR k]hiqU"T;#6Rl7U?D0^k,h/fP)} ~&]Dn?IO?p[X!m6ul0${N|X-N&W-dOsW&wzl+<,,pCws^D\we*S#ibu@QhCe3fGX.!6k|TIx*&`cz_GbJE6=W!(/tj glOM5cWL N*hbDv.ItJ[DIBG(XX7&EUU=:+i,S#|n[MVCE N|Xe1oH/`Wa?iITwM"f^@/(y8|o5c;9Vge!Yz]/d*sd6S<;1,;P}J&,39:8 !r|XquYFo'h]oP0c{yl1}ssJGH"GrM7VpCJ]wQ4=AY+-^m]ZOQM.jCPf{w&BEe>2fkNM2y,'~9'+ez^.-QlMaP)BuWZr%"[ T]Wm\y}4r(>8~Vz7}0eq/V-AMV^@5oOC&qq<6(m=':Z^u4|8e7s+]i@CHbBzH+X-]3l!LZ+UE^W?*QU/N'4;M&bX*F8Bhrb8EY|3.B+0T{!Xf] _ocT)FTX>j3XV=K3]1wv;{?kJ:h,r4[-T0M5[S*.J-j~W7.5k|_-S_7}_ooUzbaI23|5njUZ'KD7I 9xxsWD{xvrL>DI6pebvM<$AJT[Mo"$+r:y``#6E12 d+[Y5xo|[EG\}d hfz6i8Z26bb+i/_wg=A#Q9nPk@_[O~}<"&eG1h3/[ze~!Tq*nEg8Z~d_1{63_iGbN+7F= E"ae-F*r`s#T)3KO]h^* `v?{}I8w$YWmOyl}$rv/qwnF){xD\4AFUTT3T&^%FyPC,_,qz7A*k|_LhvYZ&}IlEp&wST~$M(DG?oLY7n nk0WC.%obB`!3<-,RIlVy8J9UTBe$-s:goT|0\d&POhAFDHYWm;2FVDp,CD4;,x8yT,I)rw&^LoT.i792C|5( J!Eb4;,}&v-T)D@4ir$GG\}d GoJWT|+adJsOlG6]}fWG\|J_KpdFm~;[cMmN2CgGz^n7a!.t;Z\39/hV6XD3Xm?;;DpT73@|ir$1.XHT+O}L6>U{mwY':8 vjgow^;hiksyP:r~64L&eG1H+KB6KP8)s5AVihwYc_`koGhnWe^kQtA+UZ~en?,@-nveo*uhGlB^PY6C}Afz@jXaM~63(|}jGPNYQIxw}H{-*<^k'":?S`,j.uFTcS[7}?e78SG9}_HtV2}2?Yk6]DZSPW~VW<77U AEPt 3 AEPt 3 APFN APXV 2 APrN O APrV 1 APrX } } X ARTN ASPF ASPt ASPt AUDT f f 7 B1Pt B1Pt BCTS BufT ! B # CTID CTNM CTOw CTTL CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ! B ^ DATA ! B DATA ! B DATA ! B $ DATA * U8 DATA
* U8 9 DATA * U8 DATA * U8 9 DCHT DSam DySN Dye# DyeN DyeN
DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab $ FVoc FWO_ GATC Feat / / GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE Z LAST ~ ~ ~@ LIMS ! ! % LNTD 2 LsrP a MCHN MODF F MODL 3730 NAVG NLNE ` NOIS 9y P1AM 8 ] P1RL 8 V P1WD 8 d P2AM 8 sl P2BA z P2RL 8 l4 PBAS =H PBAS 9 PCON R PCON OT PDMF q PDMF 9 PLOC 8 H PLOC 8 @ PSZE ` PTYP PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN ۹ RPrV 1 RUND
RUND
RUND
RUND
RUNT 9 RUNT RUNT RUNT 9 Rate RunN " " S/N% 9q SCAN 3 SMED
SMLt SMPL e SPAC AZ* SPAC 9 SPAC AZ* SVER 4.0 SVER SVER , , f Scal @ Scan 3 TUBE D12 Tmpr < User phAR phCH 9 phDY big phQL c phTR phTR