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###///::9EEDOOOYYYccclllvvy~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: d0fd09f0864b4873809c13f51223e7dfSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-06-05_00-57_08213130Seq1255212552_Steier_K1339_matk_Rjmadera96-Well 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"!#).36763-' !+3884,#  "##"#$%$$#%).342,$   " "'('$""$)--*"     (,-+'" &/9BHIC8+(7DNQMD90+-4=CE?3# $5CJLHB<60*"#&&%#""#$$$$&*/452)  &)'     $(%               !   #%"    #"!'*)$ $*'                  "!     !!   %+,(!  &+,*)*,/1/)   #(+-+%             "#             ")+&  './)   &+)       #&! $063)  '13-     (2=HOQK?/   '-/0.,*(&#  +34+         "#!   *25/%'-.*#   $##.670$    '1770%   '-,%    &'#  %($                               "+.,%  ',,("&2>GKIB:3//011.(     %*/21,%"#            !.55." "$!       &03-   !"!  ""            %&#    %(&                   #%$   !!              "%%"      %&$  #064*      #%"                                           #!                          "                   "''$   "                                         */-'      !!   &(#    "%&$      )+%                       %,+"                              !&$                                         #)+'      %&$                      "#"      ""%(%     !"                                                   @@AmAHA KB.bcpKB_3730_POP7_BDTv3.mobtermTATGATATCACCCACCCCGACTGTTGTAGTACCTGGGAATTCTGTTCCCCCAGGCCCCTATAAATGTGAATCACGCTCCCACCGCCCCCACGGGGCCCGCCGGCGTCAATTTTTTTATCTCGCTATGATATCACCCACCCCGACTGTTGTAGTACCTGGGAATTCTGTTCCCCCAGGCCCCTATAAATGTGAATCACGCTCCCACCGCCCCCACGGGGCCCGCCGGCGTCAATTTTTTTATCTCGC#8MZgz(6JQ^q -EZh&6Rbnz!,7Lam !-=GS_kv!.7CS`nz /;GT^mv#8MZgz(6JQ^q -EZh&6Rbnz!,7Lam !-=GS_kv!.7CS`nz /;GT^mv                                                        529S06#@&8UH5<^@EG0=6; JA]5^.$!+9$+CV=QE&'NR'+.W>AM[UF[;D>^4I^E7R</B/^L_7MW7ZD65BTQ=[W-< _b@Q@69W~BZ >Dz!HM! |  k9LlLJKX0spBM8<5A=#"csa] T*Vt&Kws% a<`=M;Iiit4]I[IZ ..0*G1N [21 7dpfd dJV,Ct_:,g`H=Yd!-(=} Q,d3Osx/88d;\LNFMUKvKk5EKp>H5<G|O.{21.OP7^34Fk n\ACATTATATGGGGTAAAAATAAAAAAATAATGGAAAAGTCCTGTGATTGTGCCCGTTACTATTTCAAAGTGAGTTTCATGTAACTTTATCTAAAAGGGCAGCCTCCACCAAACAGATCTCGCGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-06-05 02:28:44 -04:00AEPt3AEPt3APFN APXV2APrN ¯APrV1APrX}}¸ARTNASPFASPtbASPtbB1PtaB1PtaBCTS BufT!B܃CTIDSCTNMYCTOwsCTTL |CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!BDATA!BDATA!BBDATA!BDATA #G4DATA #G4,$DATA #G4sXDATA #G4DCHTDSamDySN ]Dye#DyeNhDyeNmDyeNrDyeNwDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNT EVNTFWO_GATCGTyp!!<HCFGCEHCFGHCFGHCFG==InScInVtLANEKLAST~~ LIMS!!…LNTD2LsrPaMCHNMODF ¦MODL3730NAVGNLNE`NOISOfSc 0P1AM{P1RL{P1WD{P2AM{ P2BA{{ P2RL{PBAS{{vPBAS{{PCON{{XPCON{{PDMFPDMFPLOC{PLOC{PSZE`PTYP{PXLBRGNmKRMXV1RMdN 5RMdV1RMdX>RPrNRPrV1RUND RUND RUND RUND RUNT 9 RUNT RUNT RUNT 9Rate 0RunN"")S/N%SCAN3SMED SMLtSMPL SPACMSPACSPACMSVER4.0SVER  SVER,,Satd 0^Scal@Scan3TUBEC9Tmpr<UserphARphCHphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN ¯APrV1APrX}}¸ARTNASPFASPtbASPtbB1PtaB1PtaBCTS BufT!B܃CTIDSCTNMYCTOwsCTTL |CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!BDATA!BDATA!BBDATA!BDATA #G4DATA #G4,$DATA #G4sXDATA #G4DCHTDSamDySN ]Dye#DyeNhDyeNmDyeNrDyeNwDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNT EVNTFTabFVocFWO_GATCFeat..GTyp!!<HCFGCEHCFGHCFGHCFG==InScInVtLANEKLAST~~ LIMS!!…LNTD2LsrPaMCHNMODF ¦MODL3730NAVGNLNE`NOISOfSc 0P1AM{P1RL{P1WD{P2AM{ P2BA{{ P2RL{PBAS{{vPBAS{{PCON{{XPCON{{PDMFPDMFPLOC{PLOC{PSZE`PTYP{PXLBRGNmKRMXV1RMdN 5RMdV1RMdX>RPrNRPrV1RUND RUND RUND RUND RUNT 9 RUNT RUNT RUNT 9Rate 0RunN"")S/N%SCAN3SMED SMLtSMPL SPACMSPACSPACMSVER4.0SVER  SVER,,Satd 0^Scal@Scan3TUBEC9Tmpr<UserphARphCHphDYbigphQLcphTRphTRV"O`x83E> u|#s\e?j(FsCaIqh?|cz2_'`g7FDKtRHSK%}jx7Z]et|?%9y-.^_Nx@rs{6`V5r@AKl&O3mjP/kdSP0i=qd)B*HU\A^O$['G=3/NwBx6_/<6R[Ule,rSBP^'6]`^ kS\KQp n$l^Wg#>z-nn[Y7!QnS,6dJX!Hiw8XcsG!}PlX87)*i`N-0Xwtej_@q3Luz>.9u2fkNM2y~'p9'+ez^.-QlMaP E"aeDa}r`DLXY_NwH3[`o9W'KFxz2jA<|JnFh@*;Wl&B2K4]J7U{GaC@LW&-3DLVzMymbERZpfP5!"{#[B]}P.Ea/YaWeDmAX,Pz;+hj3+'?762Z`K'HACd}Fb[q5FaT3"cX}49W+!'BA 0uz2H98$m$%MVL\bynhbe/M#yKA#2rBArc{_@=KKR"\pI2qpWA~FT~~I!8U{~D@*Wi4GG\}d _o[x}sT!lZbCd]'wyJA}*4d#0Tl_U56_n&)-%TwKBTAq;vn}4|MdwYc_`.oU.Ja$*o][:0&MSDDBI^#.gh]3uPGlOOtvU&_'SF+:bB;0FbH@O!p8NpdNmbcnPl'Z9dEe/D?*{|D$haygK >xP `v?{}I8w$YWmOyl}$rv/8NpFQlUAT/n@Z-I+TwM"f^@/(-[mMOMh7&05OPh~wA[h@tFt(#jyO~6X~3"F*+#dgU[_5:W^$OufcGHO5QfOl>o ZbCd]'GE#!#hmmfc4.=+I}S$o:/bEmKB6KP8)s5AVihx_7&OMb=KuRZ+kM7yW@"SD=y!.qK|-o*u0#|UUQ1V? MlYXjlnK[Yl`A #pJE,#VuU4zr4v4&An4/z4{ZLn*mW$Y+>0N}5!){mnjH[v]Eb,8HW>ycPJ~FT,{1:Kn6?_s2n`457^^sIdwz@{&]t[X'(A(f'PJA}*4d#kE_GY@ZWeoPTF}cyR6A;{IF}o-jAS_7ea_';_/Ft(#jvlj,DmZnyI;!H|x]0oPGlOG!>RE.$2<2oYWA8aM|It}@FB@68 EoH6iAC?-4$&)-%TwK[*oCfty5lYMI){mnjH[$.}RZ#3|>oW~!zFHyErN3W4KD@|Q$OEH< z!$w1QjXaM~PG"BXP2gXBrRLohi\`hlZZdBc'}_~:H^)!,8Kp/(ZA$den?a+b8E|W,K4vqb>H\R~.i58f{sGi8AS_9\qwH=qd)B*vH%lFUT;fQGE-`x&iG_C26R&^8o5`h]RIUwch[}k@_a'>yj6m}Pz%W%43q*)_RhGlB^PY6C}B-m2yy>T\3>>B]'ngg61C`wWsV&1RPrNRPrV1RUND RUND RUND RUND RUNT 9 RUNT RUNT RUNT 9Rate 0RunN"")S/N%SCAN3SMED SMLtSMPL SPACMSPACSPACMSVER4.0SVER  SVER,,Satd 0^Scal@Scan3TUBEC9Tmpr<UserphARphCHphDYbigphQLcphTRphTR