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? @ A B C ? @ A B C d ,,,III^^^{{{ &&&999DDDXXXaaavvv ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR B)8=?@@@??><;;976521/-)($" ] ] ] S S S v v v """../99BDDNONYYYbcblllvvu}}} < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: fdfc867428084c538aaa4e9740dcc8d5Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////
////////////////////////////////////////////////////////////////////////////
//////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB
AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA
AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA
AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA
AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-06-05_02-28_0822 3130Seq 12551 12551_Steier_K1339_matk_F jmadera 96-Well K1339_F9KB_3730_POP7_BDTv3.mobSeqPop7z
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& - 5 = F O W _ g o w Bq*o'UxxO3Z"
/W~Z0 v f [ T P N O P R T U U T R O J D = 5 . ' !
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( . 0 , $ @+kA AG6A KB.bcpKB_3730_POP7_BDTv3.mobtermCCAAGAACCCGGTCGCAGTGTCCTCGAACAGGAAAAATCTCCGCTGGATTAAGTGGGGTCCGGGGGGAAGTCTTTTATTTATAAATTTTCCCGCGTGTAGTTGGGGGGGTTTTATTAGAAAAAAAAAGAAGTTCGGCCAAGAACCCGGTCGCAGTGTCCTCGAACAGGAAAAATCTCCGCTGGATTAAGTGGGGTCCGGGGGGAAGTCTTTTATTTATAAATTTTCCCGCGTGTAGTTGGGGGGGTTTTATTAGAAAAAAAAAGAAGTTCGG % 9 H V j v %4;NYfv&@a#;Sg !4DQdr.@Tdv
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nX17s.#Z[ $`ER*HnW;\pK@Z5]W$.{*bz8_x&$ $ ^ # / M 4 + pp GezSP {OQ 10B -d 7;s rF *4 A`<8>V qNKh_ [ h[ " (h k~cN + ^<!7 > d P c < V
0 0 " 2 7 q V 4. `ZUR !tDsw`2ce'}72VP%3 @A\n"R(I 'uk)" 6e}AAGTTTTAAATAGATAGAAAGAAATTTGTTTTTTTTGAACAACTCTCCCATGAGATTTGTTTTAAAAGGACTCCACGAAATATGGACACTTATAAGAGCTAGTAAAAATCGACTCGTATTCGGTTGGTTGTGCTCTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-06-05 03:59:46 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN § APrV 1 APrX } } ° ARTN ASPF ASPt V ASPt V B1Pt U B1Pt U BCTS
BufT ' N { CTID K CTNM Q CTOw k CTTL t CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N DATA ' N DATA ' N " DATA ' N p DATA # GF DATA
# GF ,8 DATA # GF s~ DATA # GF DCHT DSam DySN U Dye# DyeN ` DyeN e DyeN j DyeN o DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! 4 HCFG CE HCFG HCFG HCFG = = InSc InVt LANE E LAST ~ ~ ] LIMS ! ! } LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS OfSc r P1AM P1RL P1WD P2AM
P2BA P2RL PBAS PBAS E PCON PCON u PDMF PDMF ) PLOC e PLOC U PSZE ` PTYP s PXLB RGNm C RMXV 1 RMdN - RMdV 1 RMdX 6 RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT : RUNT 0 RUNT 7 Rate ( RunN " " ! S/N%
SCAN 3 SMED
SMLt SMPL SPAC M SPAC " SPAC M SVER 4.0 SVER
SVER , , Satd ^ Scal @ Scan 3 TUBE F9 Tmpr < User phAR phCH @ phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN § APrV 1 APrX } } ° ARTN ASPF ASPt V ASPt V B1Pt U B1Pt U BCTS
BufT ' N { CTID K CTNM Q CTOw k CTTL t CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N DATA ' N DATA ' N " DATA ' N p DATA # GF DATA
# GF ,8 DATA # GF s~ DATA # GF DCHT DSam DySN U Dye# DyeN ` DyeN e DyeN j DyeN o DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab @ FVoc / FWO_ GATC Feat . . GTyp ! ! 4 HCFG CE HCFG HCFG HCFG = = InSc InVt LANE E LAST ~ ~ ] LIMS ! ! } LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS OfSc r P1AM P1RL P1WD P2AM
P2BA P2RL PBAS PBAS E PCON PCON u PDMF PDMF ) PLOC e PLOC U PSZE ` PTYP s PXLB RGNm C RMXV 1 RMdN - RMdV 1 RMdX 6 RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT : RUNT 0 RUNT 7 Rate ( RunN " " ! S/N%
SCAN 3 SMED
SMLt SMPL SPAC M SPAC " SPAC M SVER 4.0 SVER
SVER , , Satd ^ Scal @ Scan 3 TUBE F9 Tmpr < User phAR phCH @ phDY big phQL c phTR phTR Mr,c],~:L< zFefcXM7WX}lna] 5EX6R2? Cq4GPQFG&908]/eJ!`WBV*O.:U q<6(mu y-WPv8UUCEDFi3V[,A4#OvRio5z{,'~E{}P.Ea}_eaejp~0,{VBtn&gqr;zuhGlB5-S6C,nJ3qo8Mew.KzQ&:C=oq#g%rl(Lii$sL9,X/5`w=yBimJEIoM/V,<|ag&<{[;NR7Q@(O\?8!(nnK[vHAo?k)1mVeP *" }O^:gol%`MvO0Tl_U5TYe0tpJ_~7L3"6%4y^1Y03@a7&^LoT.iP$^=5NDzU46LD&YHE -kb+N4+0|k 1{sIRD8z{&]t[Xd:< dG#$w17h+eJ/n=Y?iQ7wDUfS*rR_V?7Z%oIlY94|j@ Ki6t{_"#kt72We<>/D?*Q| -4KD@|Q$OEH5 G,90lVinJilT>r~+:mzv&1`Z=c':Z^j?Fe6bZ( %P^gWG^D(dWKO_^h\)SQQK=`x&i9me/%ToN668d#VihGUNM4p[<2O}M=0QVkA(OHTrhV KVSFsA b5}8 v/,J6v`ZeW\j//u@8Q CKOjLH#[,bTmq/*7ag%gcmeLMaCG !9W&a:OcPC]LVn!<|'{XW,@|sF4GG\}d M;Y@iGtX[3>>B%P^gW>i;{nI YI=pI}JIXEG%w8.2_/ KRZHlEDx8_1tm9AE="TUW!\`C}B-mq5FaT9Vbr$rv/gg61CNdW)j-[YD8J)T(k"T'oA_AN;)oX6YMI)0g'^+oL.SVX*os7}Z&['Ko;X^`>EOCy KQI5m6``^,Ffn@axsA MG }OPmE$ww*{we* _ZZ^"4/GKTrQ3oS"Y|8by`AVih{3r< wvr]LaiY2]NDzU4obir8rP p'Ic|4