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D: ?(&# -)*     +    !  " %!    &                                 (#,"  !  .,8(!'1$ -%72'$        # !0;#/5'5.$&""yX$                     66OY7qt'x  i N } yS#Sf"C&CE:Zwnlh m a  X_R"&(*+^+*N(&#@ eC R Y   J( Q WxSpwv  A}&,{s < " \ 9PjT=3"%?L kgF;f)L:yv ",`e{eYqEYIF"$:"% !  !##)*"#3##*( #),%..#"'-%)8:=*,$$+   !     *  (<4J[ehxzs}lMC> "     !    (C=C=DJ09.;P,8B5@FA2@:+B#2)5+& %)-90:++03"<57<"-   ,1%<1&                                                                                                                !"#$%&'()*+,-. !"#$%&'()*+,-.d,,,III^^^{{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRB)8=?@@@??><;;976521/-)($"   ]]]SSSvvv   """../99BDDNONYYYbcblllvvu}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 0aa4499958f9420bb168eea9f5ad4bf5Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-06-05_02-28_08223130Seq1255112551_Steier_K1339_matk_Fjmadera96-WellK1339_E8KB_3730_POP7_BDTv3.mobSeqPop7z       "$&)+-037<BHNU^gq}-Ou Jp{W1 vYE;:AN`t0W ZV '-iJ@<U 3  # PL+ ]1*Z+&o8uR(2ZlVIC@BEHKKF<+{k^TMGC@>==?CHQ[i{ ,G[eeZF*w[G>FcZz_rN\SpP@<?GQ\ekoolg`WNFBH_br!)yRwA%&2AQaozzri`VMD;3*"                    !""""""#$&&&%$##$&(**)'$" "#$%%$#! !"""! !"!  !!!!"$'*,-,,+*)*+,-,)&"$*17=DMYl+b0Ab\~_? +i$_  ~  X  ` ` n,pyKRd d9Ow%[+  _ j 5 -y;*eUzo2,{ p116YCj7  '6AEC9+|pf^YURQSXcw0t8gtR)1qaz   z(L0YaZ< T k?% / [?65566642/,(&$#$'+07=BDDA;5.'"                              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J4@/A OA=AMKB.bcpKB_3730_POP7_BDTv3.mobtermCGGTTTTCGAGTGATACCCCGCTGTATTCGTGTATGTATTGTAATATTCTTCTTTGGGCTAAAAAGGTGGTTTTTTGGGAAAAAGAGCTTTTTTGTGTTTAGAAATTTTTCTCAAGAGTGGATTTGTGGGTTTTTTTTTTTTGATTCAAATTTTTTTTTTTTTTTTTTTAAAAAGAATGTTAGTTGTTTTTTTTGTTGGGGAGGGGTGAAGTGTGTTATTTAATTTGTTTTTTTTTTGTCTTAACTACGGTTTTCGAGTGATACCCCGCTGTATTCGTGTATGTATTGTAATATTCTTCTTTGGGCTAAAAAGGTGGTTTTTTGGGAAAAAGAGCTTTTTTGTGTTTAGAAATTTTTCTCAAGAGTGGATTTGTGGGTTTTTTTTTTTTGATTCAAATTTTTTTTTTTTTTTTTTTAAAAAGAATGTTAGTTGTTTTTTTTGTTGGGGAGGGGTGAAGTGTGTTATTTAATTTGTTTTTTTTTTGTCTTAACTA ):F_q (<JWcrz'?Uv+BRl '9DNZq*3@U]ir})8IY`s !,8BOXit~ !,:J[ex    ' 3 > J X f q {  # + : G S a k w   ) 3 = J Q _ i q }    & - : F P Y c k y    ):F_q (<JWcrz'?Uv+BRl '9DNZq*3@U]ir})8IY`s !,8BOXit~ !,:J[ex    ' 3 > J X f q {  # + : G S a k w   ) 3 = J Q _ i q }    & - : F P Y c k y      &                                                        &                                                     bS,c,Z EY(  ?X3',$[XXO56-b+/Ub W`( X3=;Jbb5T? ;UJ R+La-\D/.4c1H; IQ%Xbb (/9S>_J]'\Y ! Gb4A9 :R$^!. G[$QJ% .28 .M,8,8,8Q, /T5/25/c255/d+U5E+/25bU2/5/;'0\TL5=/22U?5L '2/2M/T5H#/50!O8# 9$W ~Y @m,^b" ! V%#)}E`o s GJOB;fs+: _A ~aJ0H+ [w!}H64#+|UThFXBQZVNy=A x *CSc17T, CQ>~JV&(Vu<: 05Vj m e TPO.i wpb|u6)L@pN]OU>;>]i8 Ywm4A+(&;H;\/ 4 793 3 BKp,6188Msu-e $4ITpn'yx`PTUf58}C'b*8fda_1DF N&s\jSv%^!8K8%B Q5Mad/ Q>{gT,<]]5k{ $FJXJi'dK~n_&Mk4=  5 ) E qAyO>$0%F=w%e  -*AbZ,) d:('c==^nO% )4/? vv'~C*b9BOW r9;j:Ru{_lU.vWfa!cELs ~;90< )1IqFem|' <eGpu4yub!H`jgAATAAAATATAATGGTTAAAAAAAATAATTAAATAAATACTGTTATACTCCGCCACTCTCTTTGGTTAATCGCCCGTATGGGGGAGTTCCCACGTATCAATTTGTAGCCCTCTCCTGAGTTCGAATGTAAGGGAAAAACCAAAGACTCTTGAACCCGAGCACCCGAAAAGTGGGAGGACACTAACACCCCACGATGGATAATCTTTGAGTAGAGAAATAAGTTGGCTGCGCCCCCCCTCGGGTCGGGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-06-05 03:59:33 -04:00AEPt3AEPt3APFN iAPXV2APrN 'APrV1APrX}}0ARTNASPFASPtCASPtCB1PtBB1PtBBCTS#BufT'NCTIDCTNMCTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'NDATA'NTDATA'NDATA'NDATA #GfrDATA #Gf,DATA #Gft>DATA #GfDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT jEVNT vEVNT‚EVNT•FWO_GATCGTyp!!´HCFGCEHCFGHCFGHCFG==InScInVtLANE8LAST~~LIMS!!LNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOISOfScTP1AM P1RL P1WDP2AMP2BAP2RLPBAS<PBASEPCON PCON PDMFRPDMF)PLOC!PLOC3PSZE`PTYPPXLBRGNmRMXV1RMdN ԭRMdV1RMdXԶRPrNܑRPrV1RUND RUND RUND RUND RUNT  RUNT :RUNT 0RUNT 7Rate ¨RunN""ܡS/N% SCAN3SMED SMLtSMPL ISPACMSPAC"SPACMSVER4.0SVER SVER,,>SatdT^Scal@Scan3TUBEE8Tmpr<UserphARphCH@phDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN iAPXV2APrN 'APrV1APrX}}0ARTNASPFASPtCASPtCB1PtBB1PtBBCTS#BufT'NCTIDCTNMCTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'NDATA'NTDATA'NDATA'NDATA #GfrDATA #Gf,DATA #Gft>DATA #GfDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT jEVNT vEVNT‚EVNT•FTab%}FVoc%lFWO_GATCFeat..%>GTyp!!´HCFGCEHCFGHCFGHCFG==InScInVtLANE8LAST~~LIMS!!LNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOISOfScTP1AM P1RL P1WDP2AMP2BAP2RLPBAS<PBASEPCON PCON PDMFRPDMF)PLOC!PLOC3PSZE`PTYPPXLBRGNmRMXV1RMdN ԭRMdV1RMdXԶRPrNܑRPrV1RUND RUND RUND RUND RUNT  RUNT :RUNT 0RUNT 7Rate ¨RunN""ܡS/N% SCAN3SMED SMLtSMPL ISPACMSPAC"SPACMSVER4.0SVER SVER,,>SatdT^Scal@Scan3TUBEE8Tmpr<UserphARphCH@phDYbigphQLcphTRphTR-'Icf]h_m7^1V .Uw.PmaSj4q+E[7VGJlqbQ9dHUslGW[#xjT|T6q32&L"p;I`NjYid{0PajeoI.*R@_aA+|1hygSHmT;P6[s$\o}Z'BNpW8:7F}wxSB#_{ykI0ouNbv&~^\mcJTGyr{[Kl_e5 }37RIBWjt(mID9`hp}"^wuCt(3ksKp/(ZU&benN#;98wXu0oPnBT16Y-e+&54bn%&|CO1h,9)]ZlB2K4]J7UZGfC@LW&-3DLVzMysP!b`}9(2&Sy'"jet?[)1-lHLl}gF$eD:91VuT(A|!Y<,h``z&^7a."sWir;krV=:r&YPHwZrm$.uq_6k99/qNE^O{3fJ:hA?I| )TuTF}T\Jpx _,y".9W2Bj:w`kPhL`A(^:go8m+adJs _ZZ^S_ngZ-3$tir"8|oTq}A|1D@<7>{AXG9{$5t{nn&5Gp"6QP!gFS]T'_C:rF(5,f *"[OCy KJFU'.QHT]R06`NV%]?EZX/-k'iJ4~CubWk@X=<-8 )T;Fi$t_SF`|zo;LY&PE!am&,*oe?F[Z*`E }Z&[g;z%!SNmW.$7KO],n`!3o %> DATA #GfDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT jEVNT vEVNT‚EVNT•FTab%}FVoc%lFWO_GATCFeat..%>GTyp!!´HCFGCEHCFGHCFGHCFG==InScInVtLANE8LAST~~LIMS!!LNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOISOfScTP1AM P1RL P1WDP2AMP2BAP2RLPBAS<PBASEPCON PCON PDMFRPDMF)PLOC!PLOC3PSZE`PTYPPXLBRGNmRMXV1RMdN ԭRMdV1RMdXԶRPrNܑRPrV1RUND RUND RUND RUND RUNT  RUNT :RUNT 0RUNT 7Rate ¨RunN""ܡS/N% SCAN3SMED SMLtSMPL ISPACMSPAC"SPACMSVER4.0SVER SVER,,>SatdT^Scal@Scan3TUBEE8Tmpr<UserphARphCH@phDYbigphQLcphTRphTR