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"                       *                  !                           $ !!                         !                                                  d,,,III^^^{{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRB)8=?@@@??><;;976521/-)($"   ]]]SSSvvv   """../99BDDNONYYYbcblllvvu}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 7835b6a47ca74b5dacf63c162969608eSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-06-05_02-28_08223130Seq1255112551_Steier_K1339_matk_Fjmadera96-WellK1339_A8KB_3730_POP7_BDTv3.mobSeqPop7z%##%+4@IMJA4& (,( 1Mdppf[SRYdovz}vU5 "8JRM?+%07;;;::<@FMRURK@3& !%#  $''$! $?[szgP8"$6BE>4-.9HW^[N:& 2BJJE@BJU^^UE5-/;JUWNA54?Sj~(%vtyyuz*oF{|:Ujsrmim})HnF8 +  j B5 _ \  [ l REpfL4 e@5Ccn=@yzRx; M'   znjkotwvrkd^WOE8) +4970&"#$'*--+%  !6M`mrpi_UOO[w.[G  P  wJt9|wrlaULKWp{tjZG5(%.@Zx I8flD / [ H`&B`==VD[bX?tJ3(>( B <a oedgig`VLC=9752.(!+5:8.!  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(9JZiwyV2 (& &D^mneVIA@BED>3&   '8CB7% 6HOJ:%   *;FHC>>FT_c[I5(%.=JQNC5(  $")6@HNOI<));D@3#      %*.011.' *7<9-   "   )0/&      ((  '.,"      #'$   &% '<O]b\M8% (+' %-.'   %&#  $/8<;6.&! $-8@C>6-*,5?DB6#!/63( $,,&+1.$ (-*!  $!'5;5& 7Shsri_YWZ_cec^VNE<649DSdrz~mZMIIID<42<SoiA 3=9)"04. $'"       %%   "##!%)("  !! $$  $,010/06AN\gnolga[RG<3-*'iQ@ A1ABAK^KB.bcpKB_3730_POP7_BDTv3.mobtermCAAGGAAACGATATGAAGAAGAGGGAAGGAGGGAGAAAGGAGGTTTTTTAGGGAGGCCCACATTTCTCTTAGTTGAAAACTTCCACCTCGGTATTAGGGCGGTTTCTGGGTAAAGTCTCCTTAGTTTTTAATCTTCCCCCAGCTGTTGGGTGGCTTTCTTTCCTCCAGCACCCTTCTTCTTTTCTTTTTAAATCCCACAAGGAAACGATATGAAGAAGAGGGAAGGAGGGAGAAAGGAGGTTTTTTAGGGAGGCCCACATTTCTCTTAGTTGAAAACTTCCACCTCGGTATTAGGGCGGTTTCTGGGTAAAGTCTCCTTAGTTTTTAATCTTCCCCCAGCTGTTGGGTGGCTTTCTTTCCTCCAGCACCCTTCTTCTTTTCTTTTTAAATCCCA "1<JS_jz!)5EMW`ks}'7DMSix)5CT`n}",6IQ[eqx'0;JXj{ #4BL[ir'5@J[dm 0@IWdo{   ! , 7 ? J S _ "1<JS_jz!)5EMW`ks}'7DMSix)5CT`n}",6IQ[eqx'0;JXj{ #4BL[ir'5@J[dm 0@IWdo{   ! , 7 ? J S _                                                                    H/= cS +8Y?Ab2.*(3Pd,b`0Sb+=``MS%c I) 5c9cDHE.I+MC @?U7G^T GF F_L$6FcO U$ YUVbNE3c$>M 7=/X V*OG%;C5*D 3SJ = Z]8A"_,a^M&6RbFV:2J. 62._%Ub &s }`^FX` C (-A$Z & to<" DA ,!?-1#{4dr~p*| ,'8 8/%k1 tsW+?U[[J,584-.R>7#ar\o c=o`~E#YBgZT0M4' "Ma>x+MF]}%(FWG@u) F38|*5Dze]+)=M68 *I{ yYqL8!x7-h_Gjb1;8+plp8Afp,x,Ft'p=Jp4Zz'"6~,sc[Qra3h7~4r`;{g(A(k7?*f:8r  tC  S^7BK$ToHu?0M2E x70()x $y.]C) G F-6 J"+ s A! Z*,xU/_bDDPh. oj ;!B.UA.?L?Sa72V RcU  CEwbPg\p.+A$j2,G<p\9ACCACGCGGTGATGCTCAGCAGAAAGGATGAAAGAGGGAAGATGGGGGGGAAAGCTTTTTTTGAATATAAGTCGTGGTTACCATCTAATCCCCAAGAAAGACCCCGATTAAGGGCCTCTTAACCACCAATGCTACTTTTGCTGGTCGTATAATGCACTCCCAAATACTATGGAGGTCCTCCCCTCAAAGCCCAGAGGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-06-05 03:59:41 -04:00AEPt6AEPt6APFN APXV2APrN APrV1APrX}}ˆARTNASPFASPtdASPtdB1PtcB1PtcBCTSBufT'NSCTID#CTNM)CTOwCCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA 'DATA ' hDATA '4DATA '[DCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANE@LAST~~LIMS!!ULNTD2LsrPaMCHNMODF vMODL3730NAVGNLNE`NOISJP1AM/P1RLP1WDP2AMP2BAWP2RLCPBASBPBAS}PCONPCONPDMFPDMFaPLOCPLOCPSZE`PTYPKPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  RUNT :RUNT 0RUNT 7Rate RunN""S/N%BSCAN3SMED SMLtSMPL SPACMSPACZSPACMSVER4.0SVER SVER,,Scal@Scan3TUBEA8Tmpr<UserphARphCHxphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt6AEPt6APFN APXV2APrN APrV1APrX}}ˆARTNASPFASPtdASPtdB1PtcB1PtcBCTSBufT'NSCTID#CTNM)CTOwCCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA 'DATA ' hDATA '4DATA '[DCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabFVocFWO_GATCFeat..GTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANE@LAST~~LIMS!!ULNTD2LsrPaMCHNMODF vMODL3730NAVGNLNE`NOISJP1AM/P1RLP1WDP2AMP2BAWP2RLCPBASBPBAS}PCONPCONPDMFPDMFaPLOCPLOCPSZE`PTYPKPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  RUNT :RUNT 0RUNT 7Rate RunN""S/N%BSCAN3SMED SMLtSMPL SPACMSPACZSPACMSVER4.0SVER SVER,,Scal@Scan3TUBEA8Tmpr<UserphARphCHxphDYbigphQLcphTRphTRc( !j rh[Z66YM'KvT+F4:H[);@#PA+eg[gUGGN*+j:xUIc.jCxO{VZHngM0xW#nZE35ux2]DM6mtTUk1T:29"5-z]T1S6RA0W7yLlo/&U{uclQ[vL}W$P1ObYn#pwW(Y`N!:X>zt %v1${NW8:7F}wxSB#_{ykI0ouNbv&~^\mcJTGyr{[Kl_e5 }37RIBWjt(mID9`ht1A'obU2kR@ef&e}hw@q}%Q`ALIghJ*(k;:|e}OM *R@_>j23T6Y/Xh]'p8Npdo(ktnP_I[Dd@2g|5J_@A[]"p64y^M_9ci|HZ&fNlI]uuS\cXkP?g?v!YH}&8)TK72K`rxPw% e M]Pz%y;|>EYWC.%o,n`!39G,RS_%ssaP8/GC.JuCglxsQlnWH%`WZ\9~#fW~udrVLgJRv)1mfc4hApvaUGxP=O_l;mqy=Y`ge#cme/^it>QM/N'6;\JO1L;gPZRC)O1M]^2nrD5pY-7cRb[8)FTM~j3XV=K35-x>;u4,ZBE=+HqdszeOf^DR!_T6#qLQ6?ZdwYc_`kc.?e Ml1}ssb[l"G>=?]p&k6v)ukO"yA&d}8qQgE5]U'j*-[~!Tq*nEg8Z~KI'_26?^V$Yeaejp~{>ZHm? >#veu]@|Z5hf6]X?K90#qN8>o XS_xP=O_l;mqL}4|Mdna'"`k=H<6.tJdQeK~jca1n6*17Uo AEPt6AEPt6APFN APXV2APrN APrV1APrX}}ˆARTNASPFASPtdASPtdAUDT``B1PtcB1PtcBCTSBufT'NSCTID#CTNM)CTOwCCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA 'DATA ' hDATA '4DATA '[DCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabFVocFWO_GATCFeat..GTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANE@LAST~~LIMS!!ULNTD2LsrPaMCHNMODF vMODL3730NAVGNLNE`NOISJP1AM/P1RLP1WDP2AMP2BAWP2RLCPBASBPBAS}PCONPCONPDMFPDMFaPLOCPLOCPSZE`PTYPKPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  RUNT :RUNT 0RUNT 7Rate RunN""S/N%BSCAN3SMED SMLtSMPL SPACMSPACZSPACMSVER4.0SVER SVER,,Scal@Scan3TUBEA8Tmpr<UserphARphCHxphDYbigphQLcphTRphTR