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###...9:9DDDOOOYYYccclllvvv~~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 1c066c96150843058abdbe65f78d723aSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-06-05_03-59_08233130Seq1254912549_Steier_K1338_matk_Fjmadera96-WellK1338_H8KB_3730_POP7_BDTv3.mobSeqPop7zUvp@+he^ ^:7IZV9 K7$ocKQpL."9]~jC&!9f~T86ObE749AIPZi`>2:OdngS<-1M{W-";jg@1Ai\C?RvK )RuxY2:f  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(Os{Z5&6>:- 0LaeX= %,6BIJ@,#Jri@'PtpK&  3GPJ7 :YspV<--:JTQB, !DbphL'/CG8+W{~^6"5AD@70.13/".IVN5CxuB ))+ARWO>( ChnQ=;KhoVFAFLPPOS^n{rX7Eue7  )AOJ7  !/Ofl`F( .:>5%#% )Z](%Kj{xl_RC1 7]|kH$   5pc+ &YK $Dcx~vi]UOF5Acus]= 5JVWK6  $/2,#.31' +@LMB/-KcngQ38aZ1"2:7* (,%-@F>+ 2Bvr7t%Bh|x^8,==,-EQND=BQ`cS4;U[I* >Wceem~~S%%T~m?D_eS3 #" +CYl|c( i[0'=bc-  $  *Sx}[.BoZ0    .^}I   ,V{e;  H~xil|lB:e`: 4a~W.';B7")G`fW:/J]cX@$  !&.9CF?/  #"  ,8:2" '/-$"09<80% $+./4@P`h_E! ;~c !an4 $=PYUC( AhjF 8LUQA* =gwI8GB+#Xg=%"-9:. &?ORH4  &.-%   1lG#%hY "/. !Tl/WhI6---'0%B%x#!_v@'W]. && 2[y}eB! x@c AAPAbNKB.bcpKB_3730_POP7_BDTv3.mobtermCCTTTCTCCCTGGGGTAAAGCCGGGCACTTTTTTTTGTTTCTTCCCCTTGCTCTGTAGCGATAGCCCCTTCAATGAAAAGCCTTAGTTAGGTATAAACTCCCCCTTCCTTACCATGAGTAAGTCGTGTTTAATGCGGATCGAGGTAGGGCCCCACTCCGCGTTGTTCGCTTTTTAGTCCCTTCAATGCCTTTCTCCCTGGGGTAAAGCCGGGCACTTTTTTTTGTTTCTTCCCCTTGCTCTGTAGCGATAGCCCCTTCAATGAAAAGCCTTAGTTAGGTATAAACTCCCCCTTCCTTACCATGAGTAAGTCGTGTTTAATGCGGATCGAGGTAGGGCCCCACTCCGCGTTGTTCGCTTTTTAGTCCCTTCAATG#0<NZhw!/<MYdu )5JXcr#.8EV`m~ '3=DR[gu #,6FVgx ".:DM[fpx *7DP_mz.7?KUbkv    * 6 B N ^ q #0<NZhw!/<MYdu )5JXcr#.8EV`m~ '3=DR[gu #,6FVgx ".:DM[fpx *7DP_mz.7?KUbkv    * 6 B N ^ q                                                           @(#/`C`A.^6 *FH/&/R=Z22L:H(>H;;R2VYK&U F&A?><1MQE+Y=C^<:9'U>W"D$F 'Q>9/79L;KU* UKMD#dY,N'?[a(00,@^+RV62DSKLb. _5NF )G2M X2"T7W447010.F2<-3>] }E@*(4r(#   )` /ofX> Kv]8l]~7rq48  IN/ U|'a>&  bF 9   pw)2i&u]S[ bw M<)BiBO )aD5 ]xv  9 #KtF) (;? }112u*,4eZ64RCuP&jY;_:Ei.%r 9[H%a+-@: %/5d<.)+-5yn|@pNa*X7$Vjp$/Ybgn-Z>fbw3B+fdad+MvKc8DwrE x/igU@MpZ;T,q-hiJ;vy5@R q?pQ cp'j3{d/Z&j!^#6Xx65*"Y!+ ]zDE~zx9R&52  a ^[}'Ql ;F(R-RGd.2@j?mA;99 iM 'GCGGCCCTCTTGGTTTCCTCTTTAATTTCTAACGCCCGTGCCAAGTTAACACTCTCCGCCTCCCTCGGGTCCTTTAAGTTTAGTCCTAGGTTCCCATGGTGTTTTTCCTTCCCAATGCTAGTGAGGTCCGAACCCTAACCGTCGCCGTAATTAGACTAATTAAGCCACTCAAACCATTCTTTCAATTCCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-06-05 05:30:02 -04:00AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtaASPtaB1Pt`B1Pt`BCTS BufT"D+CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA #G4DATA #G4+DATA #G4sDATA #G44DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE2LAST~~LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISpP1AMvP1RLv{P1WDv gP2AMv SP2BAP2RLv PBAS^PBASPCONPCONPDMFxPDMFPLOCvPLOCvPSZE`PTYP#PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT ;RUNT  RUNT RUNT *Rate RunN""S/N%hSCAN3SMED SMLtSMPL oSPACMSPACSPACMSVER4.0SVER SVER,,nScal@Scan3TUBEH8Tmpr<UserphARphCHphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtaASPtaB1Pt`B1Pt`BCTS BufT"D+CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA #G4DATA #G4+DATA #G4sDATA #G44DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabgFVocVFWO_GATCFeat..(GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE2LAST~~LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISpP1AMvP1RLv{P1WDv gP2AMv SP2BAP2RLv PBAS^PBASPCONPCONPDMFxPDMFPLOCvPLOCvPSZE`PTYP#PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT ;RUNT  RUNT RUNT *Rate RunN""S/N%hSCAN3SMED SMLtSMPL oSPACMSPACSPACMSVER4.0SVER SVER,,nScal@Scan3TUBEH8Tmpr<UserphARphCHphDYbigphQLcphTRphTRU[lWQXb^kOptpuD:\E+mHY`/&mayX9'-vyNJRA=57AMjCW`D/z\|DX&_lp2]m"]P"F77sI_6sKGol83p{ ]GxNu~)B*HUrG\W\lYT`M=4T*R7R8~f)NK[>Id`Po<_+MWQ}VX*oE}`Z&oRLV;);-ags5)9mW'>YP~jhM^,%bkb |{cokB}qHqHStXK#XTE-=[c}Sn2M5FiK&=RSbXW,62DmOYX/D?**s$[-p+D42F7f6QuA!9@1%ilJG82G [7P^E$62:gtGCuet$Jbr%AM&Z'K7rtlqaxkabC;Z6Ob"1{7"{2A2{UZBwE$X~}:>J5*m#mfXr9I&YD8J-o XS_9zH'zeE^O{U,HB*@Yy-4$&+VFl@i2+`'Mvf0ido!z#`5D^KfIQ.lP+Z9mHh7L6M~~hY8|kRYN4+0T{!XHTf{fzn V'o8l3XVO!DZNuOUfFhyjE^STt4)m\VD"tO(Fr~o[|!/EZdwYc_`0t;L,p$^=5NDzU4o-vNIG>S[jWcg;Z6Ob:``?(GIMfi83m ;op$SqwgE6FM;v&KT_ZO~|Qg\cK9Y\kw\czEI"m.9,(B~b) e:=K{_"#k<>Dj&['K2;+hj3jT$3o%+0Oj!Q4]"{vz6$l>o XS_d.u?zD6~hlwXs9?Ou4FS7&3-OT5ro{`N%7`h?&0(B~W^17]GhnW"K-[llj_odF4NYHY8|!I,62Z5hf6]X?K90j2oFtZ[pZX<-KGzwx?Qx]c){-*<\m(+<-F3x[R2LxC{IF}o-8xOrz6 ?63_1bTio0