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###...9:9DDDOOOYYYccclllvvv~~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 82ac835f90f64b28901d0b5ce02d63f0Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-06-05_03-59_08233130Seq1254912549_Steier_K1338_matk_Fjmadera96-WellK1338_D2KB_3730_POP7_BDTv3.mobSeqPop7z("%1;:+ <{ wZRxiCiy =;.$y }'%$5YH&qV(WIDb  q  F 9   ! m  @ : d  M  u 4P{:|FEA{ep ) e  S   1  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Fs`W/3HgOM{[r:$]  {#:p|L $ -ISO  *VCwfH5gppRm%}u,eM rdQ,XTTtl6WWF/j.w77:,& g2}4I~^wk=ZC aiBcGSuN8~M eL/B7u!}K<{+r%fsr6&pRX}V>QnW 5 !3wIvo t QlN%x72c# Bx"i n%% `8?|oSsi&c K32HK]PdSun %\!9/>8sN CpE% %I&GBVQbqqkzy+i_Z`d#s*"XL|tjrk tzS@yATCATTTAAATTAAAAATAAAGAAAATTAGTCACCATAAATGGGTGTCTTTATTCACGATTTTTTCCCAGTCGAGGACCCTATTTAGTCTACGCCCTCCACGTAAGCTGATACCCTCCTCTAGCATCACAAACAGGACCCCTGTCGCGTGGTAAGAAGGAGAGAAGGCTTGGTTGACAAAAACCCTGACGTCCTGGGACTATCACCGGCGAACCACTCGCCAGGAGGTATAAAAATGATTGTACCCGAAAGAGGAAACCTCAATCTATCTAGACCTCCTATACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-06-05 05:29:26 -04:00AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTSBufT"D+CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA #.F\DATA #.F\*DATA #.F\qPDATA #.F\DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~DLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISP1AM6 QP1RL6P1WD6 P2AM6P2BA)P2RL6 PBAS^PBASCPCONPCONPDMFxPDMF'PLOC6PLOC6yPSZE`PTYP#PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT ;RUNT  RUNT RUNT *Rate RunN""S/N%SCAN3SMED SMLtSMPL oSPACMSPAC SPACMSVER4.0SVER SVER,,nScal@Scan3TUBED2Tmpr<UserphARphCH>phDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTSBufT"D+CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA #.F\DATA #.F\*DATA #.F\qPDATA #.F\DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab#'FVoc#FWO_GATCFeat.."GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~DLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISP1AM6 QP1RL6P1WD6 P2AM6P2BA)P2RL6 PBAS^PBASCPCONPCONPDMFxPDMF'PLOC6PLOC6yPSZE`PTYP#PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT ;RUNT  RUNT RUNT *Rate RunN""S/N%SCAN3SMED SMLtSMPL oSPACMSPAC SPACMSVER4.0SVER SVER,,nScal@Scan3TUBED2Tmpr<UserphARphCH>phDYbigphQLcphTRphTR@~A67OQ1{zK90plkm$48V^p$&:?wMB>-j%;U*E^h9i_Qw|k8 <.(P-S"EnKl/:5t9z"DEoh9ZzZW-W 7W}$wFTN]%jEX@u]YMAmB] ]ddc}PMfOl>o UekB}qHqHStXK#XTE-=[c}Sn2M5FiK&=RUR6? sdWUW>|3);o]_6k4yH0@]g(=%`MU3\U5|8e7s+]i@C,TAq;vn;l|t"eAK^,0t;L,p2i#G&RcP3eZHBa+b8ENIRJ*Wi4GG\}d M5_@{&]t[X&rd+'n#wCdNmbcnPkIp?Hdsgbk1^DR!_Tr%,yPyN+8ds16;evr./b?Ay0]:jjMSDDl[zLkZA~vOE;HA>iv<("Hf.QbU`AtG>udrV|gwCiOfF{\)lOYW|_nw|]jTj.uFPqWgZ*me2&dArXx6m}3,~~+N@c'v+gK >o>s1HT+O}L6>U{mwY1XV7:>JW*z(KC,Zy`[qDr_WG\|xT;#6RlSR5[h-er5!_uJUo6X?/WZ#3|5Dfqcd o;X.>Sxp/YgZir$G,f *"y&bJ~FT,{12.Wh?7kx`rx).fcEK$J39OQJM~EbrB%P^gW7~+lF{ryE^STt4)T(wfT;C,_,qz7.5k|_^Z0b>"EJX.=DaJ2`>nX$t!TQqvLWHt$OEZA/em]ECGKXW .C5wz^`ZdD\4ApZTFS$oa$Fl@i2go~WR&^.|bEdG;_{8@hh/WZ#3|5O-U[dUDNxGq@p'Ic62v^&Gd\{diO}L6>U{mwYBTW .Nio8-I1]f\+P4avFwn&K2f_bA[]/|;vn5mr+8/Yb~;H7.N{CG2CQ>b{`$dbG_?lE8$kabCMV0|>kij7!}WQ`Q&]t[XS:.\_'[wA#Q9Tc T-*<^m9,NzVDSBrRL8~f)NKU|t2#phDYbigphQLcphTRphTR