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###...9:9DDDOOOYYYccclllvvv~~~ < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: db714e0d27984ade93715110336e7dbbSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-06-05_03-59_0823 3130Seq 12549 12549_Steier_K1338_matk_F jmadera 96-Well K1338_A9KB_3730_POP7_BDTv3.mobSeqPop7z 1 4 9 B K Q L < $ ( + ( ' 5 Y i 4 # : m D Q i 7 ) \ 8 * R p } ~ } Z . / I ] e ` Q > 0 , 5 J h t J #
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ALAJA
KB.bcpKB_3730_POP7_BDTv3.mobtermAAATTCACGTAGAGGACAGGGTACCTCCCCCGCATGTAAGTGAACTGTTTGTACCCCGCCGGTCTGCGGGGAAGGGGAGGGGCCCCTCGGGGGAGGAGGCTTTAAAAAAGGGGAGAAAATTCACGTAGAGGACAGGGTACCTCCCCCGCATGTAAGTGAACTGTTTGTACCCCGCCGGTCTGCGGGGAAGGGGAGGGGCCCCTCGGGGGAGGAGGCTTTAAAAAAGGGGAGA , < E R _ q *=Uax
&2:DKZdny
+=JVar| /8?HUZbk{ '5HXgt $/9 , < E R _ q *=Uax
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v=* T n \CCCAATTTCGTCGAATACTCTGGTTAAGGATAACGAATCAGACTGATGGAAATGTTTCAACCCTGTGCCCCCTAAAAGAACCGTTTATTTAAAGAAGACTCCCCTTTTGAAAAGACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-06-05 05:30:27 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN o APrV 1 APrX } } x ARTN ASPF ASPt ] ASPt ] B1Pt \ B1Pt \ BCTS BufT " D C CTID CTNM CTOw 3 CTTL < CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D v DATA " D DATA " D DATA " D B DATA # G< DATA
# G< + DATA # G< s( DATA # G< d DCHT DSam DySN Dye# DyeN ( DyeN - DyeN 2 DyeN 7 DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE O LAST ~ ~
w LIMS ! ! E LNTD 2 LsrP a MCHN MODF f MODL 3730 NAVG NLNE ` NOIS OfSc j P1AM t c P1RL t { P1WD t K P2AM t P2BA t t
P2RL t 3 PBAS t t O PBAS t t PCON t t PCON t t PDMF PDMF PLOC t PLOC t PSZE ` PTYP ; PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ; RUNT RUNT RUNT * Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , Satd ^ Scal @ Scan 3 TUBE A9 Tmpr < User phAR phCH phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN o APrV 1 APrX } } x ARTN ASPF ASPt ] ASPt ] B1Pt \ B1Pt \ BCTS BufT " D C CTID CTNM CTOw 3 CTTL < CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D v DATA " D DATA " D DATA " D B DATA # G< DATA
# G< + DATA # G< s( DATA # G< d DCHT DSam DySN Dye# DyeN ( DyeN - DyeN 2 DyeN 7 DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab Z FVoc I FWO_ GATC Feat . . GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE O LAST ~ ~
w LIMS ! ! E LNTD 2 LsrP a MCHN MODF f MODL 3730 NAVG NLNE ` NOIS OfSc j P1AM t c P1RL t { P1WD t K P2AM t P2BA t t
P2RL t 3 PBAS t t O PBAS t t PCON t t PCON t t PDMF PDMF PLOC t PLOC t PSZE ` PTYP ; PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ; RUNT RUNT RUNT * Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , Satd ^ Scal @ Scan 3 TUBE A9 Tmpr < User phAR phCH phDY big phQL c phTR phTR ITNn%&nJ9."G>H15KK;x^OSvqy4=(Wz`&t$SUf^3i&6j|z[vRP|ZzrA^iaHtV2}2?Yk6]DZSPW~VlWj+8w*<_swW^$oVv+rrbN<6h8b`[.b;$,PCH-p9b6mtTUk1T:29"5-z]T1S6RA0W7yLlo/&U{uclQ[vL}W$P1ObYn#pwW(YJ_93dy5*=gb_nwkI6dNNwM@B8{A)P|p$S!T,lwd-l#fr2i:}WL~?O./bE:#&PV)ob&6O`Z?5t}"^wuCt(3ksKp/(ZU&benN#;98wXu0oPnBT16Y-e+&54bn%&|CO1h,9n5N`!1;ULWyS#|Y;;y+Y3y'EV}sRCkSh!0i9>Yn2Pajeo4]tRi^as>0cPX*F8Bir!3EY$9eKoQD5pYHT"Hf.Q*s`AtI_hv"9P3&=;(=8GzUSTb<7~tr%3bbcy[*oCf!`LY&0AS_7eaPt;LOx,T2PYbWF$t FMOiiX>VL-&]S9Hgn_RDO6',>71B!Hiwe2{sGitGy;`4E~G{WhcS]p?_sJOh^&6X`??}@8jbX-@^"qrB%P^gWTb*\mvH%lFDI| sYq-f^DR!_TcS{[}7&0E(_y"LAX;:.z9W-y]l0Uo~e8HJj`$dUW7.m/J[Y^%X\HifcJkhV^!iaB69yKy\rm#ulc*{d&]?#(eaK|`P@R(RlSR5y=Y`gXBY'^n7a;A{%i7#W yZE!e~8WYH|O[nYL.wJiBwH~?p MJM*{B]9[ikI0o6Gzv&1`Zlc*+?_u';fQGKerctp(kA;M4[!f<,hm<7eaAvG9 HtWo0>B|::zWT|O\rwHB*@Y%H_TQFGa^w^8/H AEPt 3 AEPt 3 APFN APXV 2 APrN o APrV 1 APrX } } x ARTN ASPF ASPt ] ASPt ] AUDT ` ` *- B1Pt \ B1Pt \ BCTS BufT " D C CTID CTNM CTOw 3 CTTL < CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D v DATA " D DATA " D DATA " D B DATA # G< DATA
# G< + DATA # G< s( DATA # G< d DCHT DSam DySN Dye# DyeN ( DyeN - DyeN 2 DyeN 7 DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab Z FVoc I FWO_ GATC Feat . . GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE O LAST ~ ~
w LIMS ! ! E LNTD 2 LsrP a MCHN MODF f MODL 3730 NAVG NLNE ` NOIS OfSc j P1AM t c P1RL t { P1WD t K P2AM t P2BA t t
P2RL t 3 PBAS t t O PBAS t t PCON t t PCON t t PDMF PDMF PLOC t PLOC t PSZE ` PTYP ; PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ; RUNT RUNT RUNT * Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , Satd ^ Scal @ Scan 3 TUBE A9 Tmpr < User phAR phCH phDY big phQL c phTR phTR