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D L Q = a X 6 I V X [ I # G A I W > K J i B : Z ^ ] T > T a n [ U ^ b K h U S X B c g P N = W m b s f V _ ` U d f q ^ \ g e Y V T \ S : G L W 7 : ] [ = D G S Y U _ : D 4 J g N f L X a R d F I B P Y M Z m N e e K X X J L N V P Z E b W T V Q I S Q b R Y f U d e _ d ^ h j N E @ W L j F ] [ S C l K T F ] G j c i a t d H n e W } e W S O f ` J ^ a d k B S _ M \ ] \ g n g _ W Q \ [ X ` R O ^ o f Z X m e m J F a _ H i S Z D u ^ Z Z t P A I ] c h x d e f U c ^ F R ` U Z i ^ _ Q u r r c k G q | a Q S I T [ M U Q L R _ G [ T U l \ e t c l e F [ H R Z X Z S V e S S N P i f T X c c a B > e W b k M I X a < H f f a g Q V h T Q Y r h y c c r ] j a i W k H k ] Y s ` f } _ p { S H y } X Z p y g c j x u a o P v o B c B f R X W r C h a R d @ Q P N l c w b V E Y C e V R K A f f \ L W S _ ? m R N S T B H 7 C @ > N A N V U g L C f F u ] B [ P A _ g f < 7 k c N Z a L B m N K g e ^ Q a _ > \ i ` ` s f x f i b l x z p b e V c i U V m d f Z k t L M J Z Y e a p C s K H P T c Q X b a \ R W N R N [ ^ d W _ L 7 L @ ; 7 e [ ] 7 h O Z 6 ^ ` q u l m a \ p ~ c | ` b y L t a m } ^ p ~ } g n r e { l b { Z V b r [ s a W y r h z r ^ j r t x k v m r p e q r \ e z t Q i j L X D L a W j ` \ w Y X Y Q ] s o X z x j f o l u a S W P d X b T l a { e h q ` Y | | c j n b t f g z v p s w \ a w d l W ` V [ s c t m ` n k ` b ] T V O j V g h ` ] a ~ d g c t c ` a j T c U h Z Z ^ O Y v I W R c b p t l J _ n c B W b b _ b i b w \ n U [ \ k | e b d \ o j n g ` p X Y r h m d h f V i u t g \ b h R _ V Z a \ ` o b p o e j { b ` l c K | s T h n l R b C Q l q g W a d U P X N Z V ] ` Y e V W Y { \ j b c q q M ^ K ^ S e g Y R k h Z e c q _ g K _ Y N M G n f Z g O ] x N ] m Y D Y e f ] ` g f ` T H c d P | S P w d s S S ] J \ h R r i b y ] b s j f f r ] k W p h r n l o c _ j l v U P f k ` P _ X c X b p k m b p g U ^ p x b ] g ^ v } n c P a y b X V o Z v N \ Y M q _ @ Z \ d X z n n _ a r W m { [ e b l l m U ^ l ` 4 Y ` j ^ c ` M P d w a f p [ q p j w U c k R q | _ M ] c s k i ] _ i ; h p R Z ] Y R W J \ p F ` Q e ^ N _ ^ f Y ^ W | M b S C Y O U } a Y X M Y b T H h _ p D T g ^ J o N O p T \ h A J : 4 Y O h n W \ \ g g O E B W b \ F r N D ^ [ e { ` p u m q l ` i V X o ^ V b ~ g s s \ V { a V l _ d _ \ T f S Z c d n e O { [ a S Z [ c S = X V { n O L W q Y p a C ] R E : = @ R O > e ? 9 D C V X > F K g Z [ M Q = ` Y Q \ R l U [ J P M @ a d V h X ? f V c T i f Y c Y [ b K W j n a 7 h N f K i R S n P N Q O R H Q C W U ^ U U c Z b x m a T h a | ` _ l e c d Z X p I k [ R ` N K \ C R i _ M S [ p n [ \ T o m o h e \ b d Q T 7 R ` X Q K D R T Q Q ^ g U Z a v p r i W f v o Q d v k g Y m m g N d T _ S K b _ | j \ ` X i R [ S G I Y \ p S 9 : Q a a ] D 8 W B V g O \ Y n a Y P k Z c L V W P n p O Z R G J \ k i Z u n k x ^ l U n _ b v l Z R l z | l N i U ` r q d f l l _ m y h X g ` ^ r m ] [ g M k c _ p F p I t D a T E O X R b V D ] V l ` Y z J f F m f F C d U W C M X R R T N l B L \ j N M V J J P I H F U X Z O ? a s ` P k c Y N m Y a _ \ ` I X X V g ` U d j n ] W ^ d U h a v H x ] F c K F U \ O V h N R b y ` j e [ s j ] L c F ~ Z R \ W y [ f r Y c \ X z e N ? d H X R M W T b b d k A I W X h S Y i O \ M \ ` W k T J ` _ [ N M \ + [ ` I \ P Z S [ W T _ ] c o ? D Z G O O X E O E \ A P = S U ` f ` ~ e e j h ^ [ r v s l N L O ` a ] U Y W ` Y l R e z d O o P j ` \ r } W z M W ` Y R a V K V \ U k J N c S G \ n ` k X S Y N ` f M \ Y T y o d ^ S ` m d b n U U Z R \ H B M E @ @ [ ` L R N S \ O X ` s ] P } U G l [ V j q n a Q N U Z c A O T Q D D [ o N T B > \ 8 F > F k N N N Y T ^ e W Z ` S [ h K k S [ Z O W T e ] d g L R c m k l Y p T a O d S n = E w L h e l L Y O d l p \ T e H R ~ t _ \ p O d l b y R a ] n y x r ` R d b [ H c d Z X c B J o b e z q r a l b _ @ U X d V ^ B N T G f T D A O Z E | B R @ k H b U T ? L T < K P X M U ] ] i Y R F S 8 B y N \ O Z \ Y X M N N Q b \ a S N N Y [ f o O U p Y a A X Q S N i o : @ L x Y Z f V W i i H V Z _ t O J m 1 ^ Q U J F B J E ? 7 J @ M S = A Q . C : 5 Y K Q Q V H R R B @ @ O ; C 9 2 ; 6 R T G I E O p @ 1 : < R . ? W < C C W 8 ] W G F F I ] J M = D J O P _ L D @ = K ; . : : 1 8 < J 2 P L A = 9 [ V X ' U R Q K 9 N M 1 < = 9 9 ; 5 4 G 9 0 . G B G K R Z a M B J W G C N B F P 8 B I D : 2 8 > , 6 : = = E H 9 > D ^ H ? U ] S f g e Q G B f M O Q M B P < B 4 7 O R I V ; N E 4 ? G @ _ ( : I 8 . G J 9 3 B + - % 3 K 4 Q H R @ 1 X > N D / G E B @ G 4 6 F G H . P < G O P 3 N $ : > : . A K @ ; ? = E I 1 J O : : V : 2 D O @ J = 5 ' > E O 5 [ J [ J Q > J P B < D I J W V J J H = ? M N ) C B ? 5 * 2 X L 7 1 ? . 0 4 @ T 5 E 1 Y H 9 _ O f W U W M W ] > C ^ ^ ^ H X ^ D E _ N P ? 5 8 W 3 - L / = N < / < * > I 7 5 " C 7 8 $ @ 9 C 8 < = K 4 : [ : 8 I - 8 3 % > & 3 9 G * T 7 B V > % A F 8 A A O Z 9 H E T J = T H X P E e C Q E 7 J \ V N = Z h ? L [ 7 Q F E W 3 A C M 5 T U < P E * > ' # B @ D D A 0 ( 5 ! @ = @ A ; 5 > 8 & * 7 1 / = > 1 C 0 ) 7 8 A Z : ; R 9 @ M X F > 8 E H J > ? A * 6 ' I 5 Z Z 8 : C B 4 E B ) N 4 6 5 0 @ 4 B 3 ? 1 G H ) = C G ' 4 R K X 6 K F G Y D 4 ; B . 4 9 C C U I R E W B I a * ! G = 2 7 1 L A ' G Y , E B 0 2 9 0 ] R < 9 D C ; J 4 ? = / 1 / & G : - @ = 7 3 M 1 + P E A + 7 I : A F ? 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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: e8a0ba42007c40d5aaa6442ad701f56aSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-05-25_15-19_0791 3130Seq 12521 12521_Costion_K1337_matk_F jmadera 96-Well K1337_H3KB_3730_POP7_BDTv3.mobSeqPop7z~7Ykw Ek1^*`FtOF 9 l
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P z@ A#AA/A bNKB.bcpKB_3730_POP7_BDTv3.mobtermCAGGTCCTGCAACCTGCGTCGTCGGTCTACTCCGTCTTTCTCGTGTCCGTCTACTTCGACCCCCTTGTGGATCATACTTCGACTTTCTTGTTAACCGGGGGAAAGAACCCCCCCCATGTATTAAAGGAGCTCTTTTCACGAAAAAAGTGTAGATAGATTTTAAACCCAATCGTCAAACTTATTTAGTTACAAACAAAAAACATCTGACATACCGGGGCATAAGTGCCCGTAACTCTCTTCCCTAATCCACTACATTTCCGACAATTATTTAGCAGGTCCTGCAACCTGCGTCGTCGGTCTACTCCGTCTTTCTCGTGTCCGTCTACTTCGACCCCCTTGTGGATCATACTTCGACTTTCTTGTTAACCGGGGGAAAGAACCCCCCCCATGTATTAAAGGAGCTCTTTTCACGAAAAAAGTGTAGATAGATTTTAAACCCAATCGTCAAACTTATTTAGTTACAAACAAAAAACATCTGACATACCGGGGCATAAGTGCCCGTAACTCTCTTCCCTAATCCACTACATTTCCGACAATTATTTAG 2 > L T \ i w (/:L\bt )6BMXfpz '.9EQ]fo{ )8COVj~ /BS]gq
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=0QB-F /R ([_=_~@0 f#E~no b 11V$'YO)s) qQ2I"P3I J]C!ni4% 7kACCCCTTATTTTGGAAAAAATGTTTCAACACTTTCAGGGTCAAAAAAAACAATTCCGACATTTAAAAAAATAACATTAATAGTCCCACACGACCAGCCAAAGGTAGGATTTTTGTTCCATAATTTAAGTACTCCCCTCACGGTTGGTAAGGAGATTCCCCCTCTTTACCAAAGTCCCACCTCACTCAACAGTTAGTCCCGGTCGCTCTCCTAGTTTTGCGGTTGAGAACCGGGCTGTACTTGCCTGTGTTATACCCGAAAGGGTCACACGTCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-05-25 16:50:01 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS O BufT ' N T CTID # CTNM ) CTOw D CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA #R F DATA
#R F +o DATA #R F r DATA #R F DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS c P1AM
6 P1RL P1WD V P2AM P2BA P2RL v PBAS PBAS PCON PCON PDMF PDMF z PLOC PLOC PSZE ` PTYP L PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
RUNT 1" RUNT (" RUNT /
Rate RunN " " S/N% [ SCAN 3 SMED
SMLt SMPL SPAC M SPAC s SPAC M SVER 4.0 SVER D SVER , , Scal @ Scan 3 TUBE H3 Tmpr < User phAR phCH phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS O BufT ' N T CTID # CTNM ) CTOw D CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA #R F DATA
#R F +o DATA #R F r DATA #R F DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab # FVoc # FWO_ GATC Feat . . #j GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS c P1AM
6 P1RL P1WD V P2AM P2BA P2RL v PBAS PBAS PCON PCON PDMF PDMF z PLOC PLOC PSZE ` PTYP L PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
RUNT 1" RUNT (" RUNT /
Rate RunN " " S/N% [ SCAN 3 SMED
SMLt SMPL SPAC M SPAC s SPAC M SVER 4.0 SVER D SVER , , Scal @ Scan 3 TUBE H3 Tmpr < User phAR phCH phDY big phQL c phTR phTR ~.Nbmnj.W,DK >M7NJrj(R0e`EsaP=&&y:"0kfJAROX#Cc4l(Wf|Yu)+xm_'#k=sDa)x+7E2]^=,8,?qm#v6F[(vW3TA=]I:<{i(os1tog,s48h(PW[9?Db2@&4+ez^.-QlMaP E"aeDa}r`DLXY_NwH3[`o9W'KFxz2jA<|JnFh@*;Wl&]+;X$O6%nOp$eY\PSD`^BnBCmJ1y;MmN2EmIz^{5te.ERbE2(5?fqcdsTF]lOTz9NKA\@:AVVu>\&_MBSmv^Y6UziPtJdQex-U[dPz;+hj3T'7_6t;:F8/FThhsUgi;]^;CFA|g"9,)&'m#ulc*{g@]?#(lCKOJ_H#[,bTmoNH'a,k){_&jEfF:,\@\=5NDzU4E~z%QrcDpN$t.Dkln{HbX\"BmWM@A<}1X*#PE|GBW953#u[LIxLqRJ4ir$GG\}d MfaqQl&A6PG>}O!p8Npdpu#Wc4hAZG|JWIKT+}tr2LxCmqL}4|Mde_'61Qve])%,8=0?})jZ>zir8rP p'Ic62ir$1.XH`HO5QqE`|mwP@rB ]:]HE^OXv&nP_[O~}<"&eG1h3/[zGq;)y6}c8dgp3^|o:Xz zFA\|5DhECel7B]%jxxsWD@xG57'9\47.'s1Hhl@Ft/;p<.3#xFs9$-UVi,~7Cpt`3AC?-4$&#S%TwKy3ofmq*6Y?Qs?\g>?{:LKu292CB|V&Xc|LVn!<|'@4o*MK`A #pJE@^*\vJnPk=o?eU:g;TaywK[*oCf`y6n?2x`agv`k=;A{%i7#W yZE!e~8WY;`.pr$,.[G57'9\47.e6bZ( iu2Zv&1`2h`_`&1hE;qT%;5]U'V[,A4#O|!faiw`6wPjgvG9 HtWo0U:6%4sIe0