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Yn,,,III^^^{{{&&/99DDDXXXaaavvv     ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR@%''''&%$##   [[[ERRrtt(((222===HGKQQYZ[dddmmmvvv}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 90c24c1dc7b9417b9ffb7fb60e16e840Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-05-25_15-19_07913130Seq1252112521_Costion_K1337_matk_Fjmadera96-WellK1337_H1KB_3730_POP7_BDTv3.mobSeqPop7z2211/,)'&'(*+,+)&#"#&*.12210..--,,-./1333459>EKOQPOMMPTZ`fknprtx~&Dd)`:ZsW,[z{W4J c9d=/>L\p>w{nT x E v"QbT(" G b ^ N EZDm`XPC-cG+a4MrC~yn`QD;75542/-+,/5;BIOV]enx{wsoi`UI=3+%!!&0A\ Cx  s;tifhjjg`YSPOPQRRSV[afgcYJ:+  !(-/0/026=DKPSVX[_cffbYNA6-(%$"!!%3MxT!3"SvI* !/;CEC=6.)%$$$$#     $,27=DLV`ipuxzzxqfT='%Uh]  BCpo<DmfB$fJ5%  )?YtwZ>%     '7FS[\UH6$      &.32+      )3:<7.#  $&$     !+13.%    "-586/&       !0;>9.   "        !#                                       &('#   %-22-$             %%!                                                                                                                WY\_cefdbabdgiigfegjmonjfcbdhkmmmlmopokea_agnuz||zy{+QuV/x9rK Z$B\6zyGwR6\|bD& PP|[; (Gl8vO%Y0rJ vcvI6>~`oP UPvheir,Rd`F~H`NINYgw->@0r?{lcafr 'Eb}sN%u`PF?<963/,+.5ASj Bw{KwdZX[bkt|S3hAY@xc+tXA. %,27<?@?;4-+1EkJGqy]"rP0  "'-37972*"$*-.-++,/2579<BJRY\[UMC:1*%$&-7DQ]ejmnor{vQGZWVw ) 9SP][ ybL  aS=bpfC t'}obYW]iwLElmE,p4       !&&# ,=Qe{ kB#               !$%"   %((%     *13/'                                                   !                                       #(,,("   $&%                                                                                                                                                   :;;;:878:?DJNPPNMMNPRSROLHFFHLQUWYZZ[[[YTOIECCFILNNMKIGGHIKLMMNPTZbks{)Dd D V*n@1[xkZH901:HU_ccem"\/ $ q  H < c z s T % \  j 6g1t9q|K|cZco 0 zH3K   nZ]{p:{vtssu{Q(Lck`EyE.g)_]$X!a%Sq{qX3 NILxcUMGC>:63115<GWm]1fsAMpvl9UU9nu]RNNOPQTX\bhs UK``Pc4}cOhXRPOMH@6+   (08?CEEFGMYp}rQ7{;(JpxH L\8ppdXV[f|!w'gtHY'dv0dMCSA T ^ wtt dM=4.)$ "Aga:   !#&(+,,*$     !$$!   "%+5DViv|xiT;#      "$'*-/00.+))*-159:950*$                     $%#           ""##"   !                                                   "  &3;=8." ')'!          !                                              #$   !"                                                                                     #&'&%#"!!  #')**(%#"""!                                                  "#!!&*-.../0258:<=?AEINRVY\_djqy 1E_-@P`q6Mat>`jD% Q }Z78Rn7Xw sL&uA pS:%{yxwtoh_VLC<7447<DQe Bx ~Hwqoqx,Wl?yfXMC;51//12331/-,+-15:>AB@=:51.+**+,,+($   %),-./035665457<CO`x@*5$q(q`WRPPPPPNLIE@;5/)$"#+=Z5qF[C:=EOW]``_\VND9/*/@b"m9vM2#")2<HT]cec^UK?3' >}6=`\2<uVE>===:50+**,..,)&%$#"$2CS_b]P?,  1GazpXA. &043,"        '+-*$       $'+.147:?CGIJF@8.&     $&&#      $+1674-$    #(*)$      $()'#  ")-,' "),,(! ',00/+'!              #"     !##            !                              ""               ##                                                                                               _K|@A A5AKB.bcpKB_3730_POP7_BDTv3.mobtermGATCAATCATCTGTGGATCATCATTAGTATACTTCGACTTTCTTAGATGTAGTATACCGCTGTAGGACTCTTGGATCATGTCCTCGCTGTGGATGAAAGATGTATCCTTTTTGCTTTATAGGACCTTTCTCCAGAGTGTCTAGTGGGATAGTCTCACTACTTCAACTCAAGAATTTAGTTCTTCATTTTCAAAAAAAAAAGACTATTCTTTTCCTATTTGCTTGTGAAGTAGGGGAATATGATTCTGGCTTCCTCTCTCACCGTAACCGTCACTCAATAACAAAAGTTCGAATGGAGCCCTAACGAACGAATCATTCGATGGAAAAGAGAGTATCATGCTATTCCTGCAGGTATTTCAGAGATCAATCATCTGTGGATCATCATTAGTATACTTCGACTTTCTTAGATGTAGTATACCGCTGTAGGACTCTTGGATCATGTCCTCGCTGTGGATGAAAGATGTATCCTTTTTGCTTTATAGGACCTTTCTCCAGAGTGTCTAGTGGGATAGTCTCACTACTTCAACTCAAGAATTTAGTTCTTCATTTTCAAAAAAAAAAGACTATTCTTTTCCTATTTGCTTGTGAAGTAGGGGAATATGATTCTGGCTTCCTCTCTCACCGTAACCGTCACTCAATAACAAAAGTTCGAATGGAGCCCTAACGAACGAATCATTCGATGGAAAAGAGAGTATCATGCTATTCCTGCAGGTATTTCAGA#RYbnz(5=HWan{'4;AFLP\fw,7AHbl *3@MZdn'3>HRZjv+8CN[eoy'3>LV^ku "-@KVal~    $ . > J V a v    ) > J V _ i r     ( 1 = J R \ g p |   ' 0 < F P Z f p z    ! , 5 @ K ^ j u (1AQ\eq| +7?O]gq~$4AP\hs}#RYbnz(5=HWan{'4;AFLP\fw,7AHbl *3@MZdn'3>HRZjv+8CN[eoy'3>LV^ku "-@KVal~    $ . > J V a v    ) > J V _ i r     ( 1 = J R \ g p |   ' 0 < F P Z f p z    ! , 5 @ K ^ j u (1AQ\eq| +7?O]gq~$4AP\hs}   " ..*8'*% >>8!/                        "                         ""            "              " ..*8'*% >>8!/                        "                         ""            "           Z=Q9!?@^Q: @+6 ;C+.AIQ[PY^ %IF "_%A ENB:" 6 FI 0SW BW`G46_ALV_a+=7YX@#:M@JP1^Z3AP /:[ZcT&^9ZE6YYE?[Db6QPO _ BES"/8A&H,N)=PF*DRM PXF:OFT)4D=R3?>42PA^ %-)#AD>,SG;7*Q^M>-1D=ABI<6M#*@T'<P!#6 --L)b$.C ZZ!\JG-7(YT' F^K 19UGcGO 8"7CWad-I< T"ZH6"c;1a+0 L<=Z[ 0J,@U C  x&h^^mEP h T<,Z%'qMF 11XU@wQ $AuTc1^*^K*,_.a@ n!WM3U> $IJN uc= <| ){^ou63 >94e  J-J!Q;1U>W>%pY^ws#jnm"*P)WF!_S5*&q`Bkh<nPn$6Z<7(Oc^'\syH  *|}Cox'U+`Ed`e&65pQ|h^=x@~:)fzF<) 8 m (; < /4'C2O# wB<?824>  54  :pqp=Lhi}5xQpaCNk(YuAtR~mFU2p#g&)|6:#.?UHR`f8!V,h#[G9bk_$] ~eJ uL/$@Jp,d%!/ B9}ToYJz5X<Y>L.WJ.cpCFVspIIOhr]'sj?S/yJZRnIH@j sck Z)X).D 5@_mv6H<-F#e+Hr_ -y-Mo*/-9KNXKCLvnavG~`6}zl^ / L>'.n*E/ (6GJ}3&I2S{>P?1{G>[J4z@|{%Z%w Q!!i7eIu)"2e s~dx6u]0J(0Nkx+[aJ[mH5CbqfAgNTAATTGTTATGTAAATAACAACAATAATACTCGGACTCCATGAGAGAAATAACGCATTTATATAAGACTGAAATGGGCCCTTCTCTATGAATAAGGGCGACCCCTTCCCCCTTACCCGGATGTTCCAACGATCGACCGAATTCTAAGCCTCACTTTATACCACCTCAGGCGTAGATTCCTAAATGGCCGGGGGGCGTCTTGTGTGCTCCCGTTAGGAGAGGGAGAGGTGGAATCGGGTCTGACTCTATCATTCTCTCGTTGCAGGGGTATTTAAGCGTTGGTCGACATCTCCCTGAAAGACCTAGTTCTTCGGCCTCCGCTGGGGAGATCGGGTTGAGATAAGTCTTCTACCGAGTTACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-05-25 16:49:42 -04:00AEPt3AEPt3APFN APXV2APrN APrV1APrX}}ˆARTNASPFASPtASPtB1PtB1PtBCTSBufT'NTCTID#CTNM)CTOwDCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA #2FdDATA #2Fd+/DATA #2FdqDATA #2FdDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~NLIMS!!ULNTD2LsrPaMCHNMODF vMODL3730NAVGNLNE`NOIScP1AMh P1RLh P1WDhvP2AMhP2BAhhP2RLhFPBAShhPBAShhPCONhhnPCONhhPDMFPDMFzPLOCh6PLOChfPSZE`PTYPLPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  RUNT 1"RUNT ("RUNT / Rate RunN""S/N%[SCAN3SMED SMLtSMPL SPACMSPACsSPACMSVER4.0SVER SVER,,Scal@Scan3TUBEH1Tmpr<UserphARphCHphDYbigphQLcphTRphTR+This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN APrV1APrX}}ˆARTNASPFASPtASPtB1PtB1PtBCTSBufT'NTCTID#CTNM)CTOwDCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA #2FdDATA #2Fd+/DATA #2FdqDATA #2FdDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab)1FVoc) FWO_GATCFeat..(GTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~NLIMS!!ULNTD2LsrPaMCHNMODF vMODL3730NAVGNLNE`NOIScP1AMh P1RLh P1WDhvP2AMhP2BAhhP2RLhFPBAShhPBAShhPCONhhnPCONhhPDMFPDMFzPLOCh6PLOChfPSZE`PTYPLPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  RUNT 1"RUNT ("RUNT / Rate RunN""S/N%[SCAN3SMED SMLtSMPL SPACMSPACsSPACMSVER4.0SVER SVER,,Scal@Scan3TUBEH1Tmpr<UserphARphCHphDYbigphQLcphTRphTRqk+VxnD\jE@r9,V\Nz{rT'_T%`C/&m|0locKp'" H,.9)%"ef!>b7T4lpz?Wzi b!a72BZ|7W.`4A,/|8H15KK;x+;(8W)\lZh 8<"&eG1{?v2FKeS5~i2o8xOrz_lK63<%Ri24WXXp}@c[#m*Uc WUgK:=[:"yzv+r(#W3}hxv_1=|:.\_'gle#Q\WWs:rGbj8W+Sue]^WOSLj$7*pki/CF"B`@){,@Vk1$#=4]H\#D\mvH%lFU~ZYWm;2FV]#[,e/8I[X1EDE?]Rc{_@=K{_"#kt72Wer]LD_!lEH|x]0oPGlOG!>RE.$2<2oYWA8aM|I:}OUB9D!K#Z]Pk=o?eU-:.<)%,8#V>Ax`X~lTmt.qK|-o*.AQ>UA!%EeKOWQ`QV-!w2l89+uTE#!#hmmfc4hAo?e3{0+oiw87[%Vr6R[Ule,rS]RnjH,3LiPtj glX ,cW`}F)h>?[ha_DMOFU>^[?|&Rv3672Z)~6bI.J:*yEwiOM#Y\V&&OE6OLogXY@ZB 3O{ ~VJ(/on}sF{AR8=(V-OGC#!^MrrGpwQ.fB=n\N;[6_b.O_l;{IF}o-,(B~bHjAt3_iP-2ZE<}DU$~~8WYhY8|k~_D}<|hG6`VsIefaqQleA./q}.}:>JWT~Q;maHjlUOE6OLhcDW4KD@|v^&Gd\{diO}L6>U{mwYBT3?^NHEDX+9m#k@l'Z9d[TCs]jL'#k=sDa)x+7E2]^