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"     )&#        Yn,,,III^^^{{{&&/99DDDXXXaaavvv     ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR@%''''&%$##   [[[ERRrtt(((222===HGKQQYZ[dddmmmvvv}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: f89ffd630ea04a05b18e900086466f27Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-05-25_15-19_07913130Seq1252112521_Costion_K1337_matk_Fjmadera96-Well K1337_E10KB_3730_POP7_BDTv3.mobSeqPop7z%1C\v#174* (3:<8. 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""!           !!         !##                                "                                                   #                                                                   &*)$                        (--&  !%%"                                                                                                                                            Ie@VA{AFAdKB.bcpKB_3730_POP7_BDTv3.mobtermGAACAAATCCTGAAAACTCCCTCCCATACTTAAGACTTTCTTGTAATTCTTGTCTACCCCCCCCCGCCCATGTGCCCTCTTGGTGCAAACTCTTCGCTATTGGGGAAAAGACGCCTCTTCTTCGTTTTATTACGATTCTTTCTCAACAGTATTGTAATTGGAGAGTCTTATTACTCCAAAGAAAGCTAGTTTCTCTTTTTCAAAAGAAATCAAAGGTATTCTTATTCTTATTGCATCGGATGTATGCGAAACGAATCCATTTTCGTATATCTACCTAGCAATCATCTCAGTAGAATTAAGGAACAAATCCTGAAAACTCCCTCCCATACTTAAGACTTTCTTGTAATTCTTGTCTACCCCCCCCCGCCCATGTGCCCTCTTGGTGCAAACTCTTCGCTATTGGGGAAAAGACGCCTCTTCTTCGTTTTATTACGATTCTTTCTCAACAGTATTGTAATTGGAGAGTCTTATTACTCCAAAGAAAGCTAGTTTCTCTTTTTCAAAAGAAATCAAAGGTATTCTTATTCTTATTGCATCGGATGTATGCGAAACGAATCCATTTTCGTATATCTACCTAGCAATCATCTCAGTAGAATTAAG#.7?HPZhow+5BMU^it)4?MVjw!-9ERan{ "-8DO\gp{ )4?JValw ",8CQYcnx $0=MXeoz#.9CNXcnx  ! ) 5 ? H R [ f s    / ; J T ^ g p w  " . 5 @ J V ` i t    1 > E Q ] g t }   #.7?HPZhow+5BMU^it)4?MVjw!-9ERan{ "-8DO\gp{ )4?JValw ",8CQYcnx $0=MXeoz#.9CNXcnx  ! ) 5 ? H R [ f s    / ; J T ^ g p w  " . 5 @ J V ` i t    1 > E Q ] g t }          $#      '     (8#"""  "  #      8"!#     '#                             $#      '     (8#"""  "  #      8"!#     '#                      U ?^JI:?2D^=:IE)"cDcScB!'69<S_aM,/P+ca<O92&+<P& (4K4 MJ:,EOW,IH1:<+ ,W,SJ2ZE0N-&EM YQFA<@. .06-B(3:_W(5Q%FAN;K >(? HI/D/ L2O%9J'\K-OR+EF.\ZN)C!`.b=^\G_DGQ.Y##>3$*RAG*4/8"@$N-=YEUEWE/$ \4:>\7A'Y=X&R4Y9%.U8!1] O(0Y zg>Tyu;/\,s$  D#>uQo3K-bbLWsT<H Sko5* )vU= Z: \lzCi'n@ l#"N  tt Ys3q+MYP8S6WCz?AOW Nig,= "/:]f;~Afb@K=M _Jsr\:fem (V'?p=a{c)**1L   H :Q$@^rSGc`/>2qS*Da,o.t^[7Wx\4IONU7OZ,RA W & !  W5%T# 9*A3VEL6=( )n KRv5J @klv8&Bplp98;,BV?_%` N[Ir"'Jf!X}P'<7Yp+Ag'@ |\Kp7iJ-!nt~oe0T,pDU~,aG+$Lc5x,z^ !DIME^Y? K aK=lpYo>)kkx6X}hC]0RPxo4`/<S S f3X&7j;wdE6?Ss4j|T2wnao=CTMo5!$#.1x3gs @Bcg,o ?ud\-2urcw?>D&6w^qD01&_CbR-*w./q6' <{ !0(AGCACTTATTGTGTTTACTTACTGTCACAGATCACTCAATCGTGTTAAGGATAACCTTTAATTTTCGGACCCACGAAAAAAAAGTGGGGACGCAGTTCGCGATATGGGGAGAAAACTCCACCTTCCCCTCCTAAGGGGCCCTCTTTAGCGGGGAAGGGGATGAGTAACACCACACTGCGGCCGGTGCTAGACGATCGAGAACCTGTTTTGACCGATGGCCAACTAAACATAAATTGGAATATGGAAACCGTTTGTCGACAAAGGTGTATAACCTGGTCACTGTTGTAACTAGCGCACCCTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-05-25 16:50:57 -04:00AEPt3AEPt3APFN APXV2APrN APrV1APrX}}ˆARTNASPFASPtASPtB1PtB1PtBCTSBufT'NTCTID#CTNM)CTOwDCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA #eFDATA #eF+DATA #eFr`DATA #eF*DCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANEHLAST~~sLIMS!!ULNTD2LsrPaMCHNMODF vMODL3730NAVGNLNE`NOISP1AM,X P1RL,X P1WD,X?P2AM,XP2BA,,GP2RL,XPBAS,,[PBAS,,/PCON,,cPCON,,7PDMFPDMFPLOC,XPLOC,XPSZE`PTYPLPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  RUNT 1"RUNT ("RUNT / Rate RunN""S/N%SCAN3SMED SMLtSMPL SPACMSPAC SPACMSVER4.0SVER SVER,,Scal@Scan3TUBEE10Tmpr<UserphARphCH*phDYbigphQLcphTRphTR\oThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN APrV1APrX}}ˆARTNASPFASPtASPtB1PtB1PtBCTSBufT'NTCTID#CTNM)CTOwDCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA #eFDATA #eF+DATA #eFr`DATA #eF*DCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab&VFVoc&EFWO_GATCFeat..&GTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANEHLAST~~sLIMS!!ULNTD2LsrPaMCHNMODF vMODL3730NAVGNLNE`NOISP1AM,X P1RL,X P1WD,X?P2AM,XP2BA,,GP2RL,XPBAS,,[PBAS,,/PCON,,cPCON,,7PDMFPDMFPLOC,XPLOC,XPSZE`PTYPLPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  RUNT 1"RUNT ("RUNT / Rate RunN""S/N%SCAN3SMED SMLtSMPL SPACMSPAC SPACMSVER4.0SVER SVER,,Scal@Scan3TUBEE10Tmpr<UserphARphCH*phDYbigphQLcphTRphTR'%N`!2Fu4WyYZ(Wa:nuXK|+U17ujZj_!p-">R2`Yyb oJH.`Ep2=TgVSQ<}LgU!IZHp#)+KT[^[G^%E:"*l#qN8>o Ueh,9n5NJw=;SocJyw*6TdO{7I{4^PrtC[J19D5mr+8B9.~;{lUz{19se0:=y=&e=1&!rEV(V-IAG&e"i/ud?7OBmxCB#v6|k.j H_H-CI;{dY7cI*]5cP}Gs+OStxHlYxC?(cU/~!-,'bF4$T9tR3#=cC0^,!Sk2%vAfIx+C!K4#]BN $`?"*JIf7Qv6,MZ@Y+P'#9r??8r]f.iD4ZI,|Q|T{\K71P3;fTR[fRr;-6U@;EGCfQ&HQ$ 0603XXbLmX;9 |.W162Q$OEHO6z7B-_8Es{sMV^@}Ouxg>33.BkST{!Xf] MzQd)l'[)VBe$O!&HoCdNmbcnPn&p;s_|g+XfT;KB{s2fo;LY&03t0mP`k:!.tkj^4E2s JX0bt-] 5y$47y 4orKOR`{A,}@1%i`+|*f~B]39u!2'z*Rr,Z:X=<;s_|g+VFl@i2go~WR&^.9,(B~bHjAt3_iP""#]eRWJT|eiB]%j'k0WC.%ouO{9X6!7&o.ja8G$l=b d[qm#vRRC(vJnPX=<-8 )S-F3x@M9X`|W"[XYTZM%SmvlM@=K}VX*os7}Z&[Eb4;,#|>WT?@\xoF5K.Ijd!FwMz&4-@6|k.j,PCH-p}*4d#ky`[qDr_WG\|xT;#6RlSFy!{]-j5OPh~wA[h@t?"\=QtAcP@~~JBv+c8*+C!K}Z'BNp3<(+(aQQxnJilXS:Q&:C!g61CfFc':Z^mDt4)Mz99#mJmIzp7KRV]}dc-|Hp5^KfoQP-2ZE<}g6M>PzMWhc'kp?_sJOhoN^U`dbM;Y@iGu2tG=}V7V=y`6@NBF{l9cGY@ZWeoazNpP S!oS_,y".9+5SGKI'_26tKR"\3|>oW~!Eb4;,|`Dp>H,o}Qm4'p=R .*}Lq{F}u^zq+AM&Z'K7rtlqaxkabC;Z6Ob"1{7"{2A2{UZBwE$X~}:>J5*m#mfXr9I&YD8J-o XS_9zH'zeE^O{U,Z:X=<;3On&+VDyK{t61h67A"yV+XZ