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'                                                    $                                                                                                   #              $                                      %                          #                                                                                  Yn,,,III^^^{{{&&/99DDDXXXaaavvv     ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR@%''''&%$##   [[[ERRrtt(((222===HGKQQYZ[dddmmmvvv}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: f20623e2124e4993b9bde0fc675eb7e5Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-05-25_15-19_07913130Seq1252112521_Costion_K1337_matk_Fjmadera96-WellK1337_B5KB_3730_POP7_BDTv3.mobSeqPop7z()/:Nfzxst~vcXYew $ +/+''1BT`_Q<*"(:O^c[J5! 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"<OTL;,#&/792#  )35, #3=>9317F[pzl]OC6' .@JJ@0  '4FZioj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l;@AAFoA KB.bcpKB_3730_POP7_BDTv3.mobtermAAGGATGGTATTGATCAGAAGCCGCCAGTTCAAAAAAATGGGATAATGTAACACCCCCCCCCCCCCCGACATAGGGAACCTTCCCGGGGAGAGAAGGACCCCTTTAGTTTTTGAAGGTGTTTCCCTCAATATTATTTGTGCTTTTTTTGTCCACCAAACTCTCTTCTCTTATTTTAAAAGAACCCAGTATTGCCCTACCCATGGTATGAAATGAGGAAAGAATCTTTATGTTTCTTCTAATAAACCTCTTAATTCAATCATATCATCTGTAGTCTTTCTGGAAGAGCACTTTCTTTAAGGATGGTATTGATCAGAAGCCGCCAGTTCAAAAAAATGGGATAATGTAACACCCCCCCCCCCCCCGACATAGGGAACCTTCCCGGGGAGAGAAGGACCCCTTTAGTTTTTGAAGGTGTTTCCCTCAATATTATTTGTGCTTTTTTTGTCCACCAAACTCTCTTCTCTTATTTTAAAAGAACCCAGTATTGCCCTACCCATGGTATGAAATGAGGAAAGAATCTTTATGTTTCTTCTAATAAACCTCTTAATTCAATCATATCATCTGTAGTCTTTCTGGAAGAGCACTTTCTTT '7CThv #5CTdqx #3=Qcw#6AP[fw",:Mbs(9FZcny".9CQ`q,;EVhs'1?OYet   % 2 : F Q ^ n x   , ? I R b n w   ' 3 D O [ m z  + 4 > I R _ k r    / 7 F Q Z f u  )5@MXiv~ '7CThv #5CTdqx #3=Qcw#6AP[fw",:Mbs(9FZcny".9CQ`q,;EVhs'1?OYet   % 2 : F Q ^ n x   , ? I R b n w   ' 3 D O [ m z  + 4 > I R _ k r    / 7 F Q Z f u  )5@MXiv~                                                                                                                           &#Md^PPI!IEJFb(PcR[, 58$D TXc<)5CBE]#G7"A'*Q+dTb8[$GW>[8 Od0 5T@"UYY>&,][37)@?RF LK1$ Q 5P)++DC0b4^4C'V_U_@d9<K;C7[1O()TdHD +R73B7N9K;*,W0,MHM[;VY!^H8>X.2NFW*_A%L L:cZDCVSXP*!-I ,:!@\T"I@ M8K>X/WIUc CC;I 2a?FJ45!9I&x@>gKe]MQR&s o e  ] rHM E 6X5_;{%n@[At ij5gL,YmI ae rcvSj>)}B+ #bOqyaeX~_5}zpzA0 >rh m]3"acJGFo"@.BK1,F9r;@$"^<+h,1eQ3wL0Dy/ JD[;Tj%Gs~Y)AKpi$(cr O3U5#wU9/J1?LH,BSJC 8 %!"  ! Pvpt =p7F,V h;+]gYZj<Xl,,)K/Fqb8tAMKIK*:O?_%!.{@Q8?$+L?gWwR9'83],|0-=8>h@3d`Y}$|F5THkVf(%+8X;HMKU-\ -\9G> ;Vk/A%J~&N$4Dc=E.gF^,ZCCATTATTATCGTGGACTTTTATTTATAAAATCTTTTTGATTGAGTAAACGACTGATTTGTTTTTTTCGATATATTCCAACCTTGTCCCGCGAGGAATTGTTCCATTACAAAACGCTATCCCTTTCGTGATGAGAAATGAAGACGCGGTGTTTAATGTACTATCCTCTCCTGCCCCGTTAGGAAGGTGTCGTTGGGCTTATGCAGGGTTGGATGAACTGACTATCGATCCCCATCGTCCCAGGGAAAAACTTACTCTATGATATTATGAGCAAGAGAAGTTTCAGAAGAACATCGGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-05-25 16:50:30 -04:00AEPt3AEPt3APFN APXV2APrN APrV1APrX}}ˆARTNASPFASPtASPtB1PtB1PtBCTSwBufT'NTCTID#CTNM)CTOwDCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA #GDATA #G,DATA #GtCDATA #GDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANE-LAST~~LIMS!!ULNTD2LsrPaMCHNMODF vMODL3730NAVGNLNE`NOISP1AM(PP1RL(P 6P1WD(PP2AM(PvP2BA((P2RL(P&PBAS((PBAS((PCON(( PCON(( PDMFPDMFPLOC(PPLOC(PFPSZE`PTYPLPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  RUNT 1"RUNT ("RUNT / Rate RunN""S/N%SCAN3SMED SMLtSMPL SPACMSPACSPACMSVER4.0SVER lSVER,,Scal@Scan3TUBEB5Tmpr<UserphARphCHphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN APrV1APrX}}ˆARTNASPFASPtASPtB1PtB1PtBCTSwBufT'NTCTID#CTNM)CTOwDCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA #GDATA #G,DATA #GtCDATA #GDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab)FVoc)FWO_GATCFeat..)GTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANE-LAST~~LIMS!!ULNTD2LsrPaMCHNMODF vMODL3730NAVGNLNE`NOISP1AM(PP1RL(P 6P1WD(PP2AM(PvP2BA((P2RL(P&PBAS((PBAS((PCON(( PCON(( PDMFPDMFPLOC(PPLOC(PFPSZE`PTYPLPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  RUNT 1"RUNT ("RUNT / Rate RunN""S/N%SCAN3SMED SMLtSMPL SPACMSPACSPACMSVER4.0SVER lSVER,,Scal@Scan3TUBEB5Tmpr<UserphARphCHphDYbigphQLcphTRphTRFB}}Cg/D&Wr`s;bqz^]/r$^.>6pe:*>^Jp@48Pl: &Pwi~69b[dWUlh{.4eY\`;w>xPqL6`nCRNX}a/JBcw*jEth@t#Rt+7+:}JcLL-GIA2oz:4JA^V\h}c%kU?-}U&0;S3ZfYUgB\(<({]kId`Po<_+MWQ}VX*oE}`Z&oRLV;);-ags5)9mW'>YP~jhM^,%bkb |{c|5<&n5N`!1;U*W]S#|Y;;y+Y3y'E3ro{`qaL`hlZZdBc'@wMXC5K;@WJG7jVlG0$_9'mSTve!@;Ps>4]F%65r#W3}hxv_1=|8Ifz2wHiD(g=0ia}yI?t_tG|T`S'_[PTcM#yKAu22`ArIxT6e&?bFW3|>oW~!Te4;,: TZc:=?t)b"#\Fr+4WA*mPp$.jc_T3 #pJE,#VuU,YTl_u4TYe0tpJh&j43ofR5*6Y?Qs?]t~1{[q?Eb,T2|5iZFod>7:I12mxXYnuPQh0{!X<(&}J39OQJM~6Hrd+v/gg61CM#Y\VL&p?)_QQe|vH[p!hjqf[MH-YtDSYcJ|_nO*r<4,v`Ws(Ou]9g~~hY8|kb+N4+0Oj!Q4M"}JMzR9vAMV^@E P:i~8m+adJsOlG6]}fWG\|J_b.O_l;,[[`7VAd#RBK9JX@{F`!~6I57%6C}B\I/v]-@6|k.j,PCH-pC+lkfc4hACI8-W=;OF>BrRL8~f)NKo-j5t9z"H~:HziPtJdQex-U[dPz;+hj3T'7_6t;:F8/FThhsUgi;]^15F:NI"9,)&'m#ulc*{g@]?#(lCKOJ_H#[,bTmoNH'a,k){_&jEfF:,\@\=5NDzU4E~z%QrcDpN$t.Dkln{HbX\"BmxPQ1V'.$b=}$A>AR8d}$7|:zNz~vdlc*+?_u4ZfoQe=N^tpups'MvK}7V,2{+w" 4*39nbeWo0<:LP@]Xi-]js3W4KnuPQh^>kij7!}WQ`QEJ9."G>}l)-l&T|*1fCy]_^ave%e0MSaywKB3x[f9;"Y+Ax8aPI^QJH:,ptJdQex-U[dPz%I!|spKa3gDkS${N|X-N&W-dOsW&wzl`O_mzAEPt3AEPt3APFN APXV2APrN APrV1APrX}}ˆARTNASPFASPtASPtAUDT``7B1PtB1PtBCTSwBufT'NTCTID#CTNM)CTOwDCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA #GDATA #G,DATA #GtCDATA #GDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab)FVoc)FWO_GATCFeat..)GTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANE-LAST~~LIMS!!ULNTD2LsrPaMCHNMODF vMODL3730NAVGNLNE`NOISP1AM(PP1RL(P 6P1WD(PP2AM(PvP2BA((P2RL(P&PBAS((PBAS((PCON(( PCON(( PDMFPDMFPLOC(PPLOC(PFPSZE`PTYPLPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  RUNT 1"RUNT ("RUNT / Rate RunN""S/N%SCAN3SMED SMLtSMPL SPACMSPACSPACMSVER4.0SVER lSVER,,Scal@Scan3TUBEB5Tmpr<UserphARphCHphDYbigphQLcphTRphTR