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F W W V Q N [ S Y M I Z N \ R N A < I V R Z _ O ^ F a \ J H I O < B O J H L L O d D B S ] m X a \ b Z V ^ z P ^ X z } { } d x x g h j J I T H G c N B G ; X P O @ N \ > Q X P = 6 S D ; A D 0 = 8 9 8 ; A = ? * 1 7 / / B B ? < B 6 A + < 5 < > 5 2 D = 8 . E ; C = L < ? M D Y ^ d i s ~ u ` j c T I J J H < H H 8 4 W O J B $ : . 2 F 1 - ; 1 / < F 5 5 + ; = 1 1 = @ E U T a T d s n j y l d l b y Y V W O F X \ @ P @ 7 I F Q A > 2 K 2 7 H 6 > K Q $ > D 7 : 9 7 4 < G ? 7 @ . B N J E E E 9 3 U 1 M ^ H k N ^ O K ` i \ \ l c g f Y \ [ a T f ] R T P I Z G L a V [ Q c c j { v n p w Z ` T ` U I m W K Z e Y R a V l L @ J U M R B H M H M A > > A 8 7 4 D ? B 3 K 7 < F U 2 F = / P B F O A J O J ? K D N U K @ M V T @ P f K R V J U P L V B X S K Y T R > U = I A N U K H M C D L > > ; Q B D ; ; H : K 7 = * G H 9 ? D ? - 6 A 8 : @ V = = E 9 R H O B Q ` Z ` y u x W x d W M R m G Z F ^ O O I G J 7 G J = H N _ P F F < 5 9 3 Q 9 : A A X 8 = D 4 0 < @ Z M N Q G Y M E J S M F ? R ; 2 = H A 7 F > : > A C E 0 D O F Q = I M C ] \ a | w | s v n | w g [ ` W O \ O L 5 < > : L C 5 D . D 5 7 C 7 e ^ L k b m w } l m V R a _ O 4 5 O 3 . @ E 8 < < 2 ; 4 Q * G 1 @ 5 F @ 1 I G G G ; 4 A = 3 C D C 9 ? 8 ) A ; C @ 2 G K 5 8 A 3 I C @ O < C H H = F D = A K E @ I U K U W H U [ O I U C P R D M 9 ; ; - < 2 % 3 = 9 4 ) 2 ' 4 7 3 2 M + F ? = = < > - ; < ? 1 M Q R U O W j x z p v x r q { n Y o m P X Y E H S P ` g V ^ G W X T J K Z H Y W T @ < B G Y T G W Q > N K R ` L M H W U E J S T \ N W W @ I J O T S S H C L 6 : C 7 K @ 9 9 2 G B C 8 8 D G 1 L B R E 4 P K @ @ 9 I K K [ M P V S ` \ f W Z _ L ` k b Y ] j o c u y u o b Y ` ` M [ M G ^ + , ? M G B O S M V [ S [ ` I Y R b P _ W I F X R ] < L F W ? I F T Z Y z _ i u h \ d a b _ \ e U L _ Q G A C I K I F 3 D ? ^ P : = A F 6 H E I P O M V G P = P J B E M M ; ^ Z O D _ [ I L ] Z a Y O I J B K Q \ V ` Y W ] f f a d n g z l u l r V P C Z A O > J ; M * 4 2 ) 3 . + 4 * > / = . - @ : E L b f Y p h x r x k r z b Y _ S C L L 6 J 2 / ' $ 0 5 6 : 8 . 2 6 0 $ > 5 * D : O j \ \ n b u p z { e ` o Y D P O > M 6 @ A < L = K < ; 2 ; 7 9 5 8 : G 5 H = E 7 : 6 6 N F D O 0 : : / < G 8 J 0 > . 7 1 0 , , A 2 H / 4 1 @ ; > = 3 1 = U A C ) 7 = C 4 C 6 = G N L \ _ r i i o o v n S h l V ^ I W I Q E D ; F > d Q S X d j } c x y | y u f j L i h Z D A ; = 3 F N 2 > ? > C ? 6 ; = C ; = N @ @ M @ ? H A 0 I J B 7 V Q H @ D E 9 8 9 N M P , < [ 7 8 2 3 " 6 < % + , = = 3 A A : : 2 H 8 % 8 + . D ; 9 B ? 9 6 J 6 ; L Z 8 _ U k ] _ ^ p t m | y s q z v b f ` c ^ Q _ [ H U F H C M 5 8 % D 7 : + : : ( / ) / , 4 6 3 1 L H . & , - ; 3 7 H 0 C A = 9 = < 3 3 = . E : H / < J > 6 5 @ F N A < C S O 3 J 7 5 V D H ? L - I O B < @ I 3 A 7 + ; . A 8 M ? / 9 0 H < A > 5 - . / ' ' + ? P @ % : ? 6 B 8 2 C = 9 0 4 9 = H < 1 = ; 6 3 B O > / / 7 * 9 ' $ A ' < 8 B R H E K 3 2 F = E > I E D C S d g r v x c { { k u a Z _ f Z ^ W r [ S f N H [ J \ C C G K H Z I Y G > I 6 B A I O C ? ^ H [ c ` ^ b h u m x y | | u } z v e f c X a c F I A T C 5 < F 5 4 L : 7 F / / 5 ) 6 B : > 0 7 H 6 B < ? K ( F < . 6 @ F / . ' 4 : 5 . 3 8 6 8 @ . 2 5 , 0 , # / 0 5 9 . / 5 4 : 9 ( : 1 1 % ) % 0 & # $ * ! - ) / 1 1 ? 5 , < I K < 9 D ) F I K G G C L T M V e O Q Y Q L L M L [ F B J B 7 [ Y L U n d T i x t } t o } d w s M Y M S ? ? 5 D D 2 & 3 , 0 3 5 , ) = ! $ 4 5 ' / 0 ) ( ) ( - " , 5 5 - = 0 = & ' 1 / * + F 8 > 2 ; 3 D + L 8 - B 0 6 3 3 - , 8 1 9 7 F : 9 K M P 2 G > 7 H = 8 7 = 6 6 ; = B 1 # " 6 & & + % * ) ! ' / " ( + % ) 2 ) $ # # : ! 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Z [ [ R R R t t t (((332===HHHRRQ[[[dddnmnvvv~~ < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 1b38a08db3db448095a9aa949aec9558Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////
////////////////////////////////////////////////////////////////////////////
//////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB
AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA
AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA
AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA
AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-05-25_13-48_0790 3130Seq 12520 12520_Costion_K1336_matK_R jmadera 96-Well K1336_G12_RKB_3730_POP7_BDTv3.mobSeqPop7z $:LZfr 7Me~0Ru
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kv~ ]lj% ]v\ _ [ xy~ ` [wsC {S ; d^Yn x g-a )YCGGGTTGTTTGGTGATTTTAACTGATGTATAATATAGTAGGTCGGTAAAGTTCCGAAATAGCAGCCTGCACCCTGAAAAGGTTATCGGTTGAGACCCTCCTATTTCCAATGATGTATGGAATGGCAGGACATAAATATTTCCAGATTTCCAATGATCCGTGATTAAGCCGGGCCTCTTGGGGCCATCTGTCGAGATTCCCATAGGGGAGGAATGGATGTAGAGAACTTCACCTGGTTCAGGGGCGCTGTCTTAAGGTTTTGTTCACAAGTAACCTGAAGCCTCTTGGGGAAAGGGCTGAATTCTTCCCAGGGGAAACATCACATGGGGGGGTCATTGACCCTTTGGAAGGGCATACCCGGGAATGAGTGGACTTCCCTCTCATAGAATTCAGTAGAGATCCCACCTATCTGGGAAGAGAATGGTAGTTGGGGTTCGAGTGAAGAAGAAGACTGCTGCAGTAAATAAGCATATCGGAGAAGAAGACCTTCCGTTCCCGTCACTAATGATGATATGCTATAAAACGGTAGACTGGATCCGTCCAGATTATCTCAACATCTTATAGTTAGCGCGTGCGAGGTAGTTGACACTGGGTGGATTGTCATCTCTTACTCTTATATAGCGCAGTATAGCAGAGTATAAGCTCTGATATCTGGACATGGGAGTTCTCTGGGCTCCGTTAACTGGAACATACAATAGACTGATGACAACTCATAGAGGATATCTTATTGCGCGAAGAGCACTTCGATCTATATAGAGGTACCTGCACGGTACTTAGTAGCCTCTAGATGAGATACCTAAATTTAATTAGTAGCGTGGTTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-05-25 15:20:02 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS =! BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA #? F~ DATA
#? F~ + DATA #? F~ q DATA #? F~ DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE T LAST ~ ~ ; LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS P1AM 3 f P1RL 3 f g P1WD 3 f %3 P2AM 3 f 1 P2BA 3 3 8e P2RL 3 f + PBAS 3 3 PBAS 3 3 PCON 3 3 4 PCON 3 3 PDMF | PDMF PLOC 3 f PLOC 3 f 5 PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
0! RUNT RUNT RUNT Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL p SPAC M SPAC SPAC M SVER 4.0 SVER = SVER , , n Scal @ Scan 3 TUBE G12 Tmpr < User phAR phCH phDY big phQL c phTR phTR { This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS =! BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA #? F~ DATA
#? F~ + DATA #? F~ q DATA #? F~ DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab K} FVoc Kl FWO_ GATC Feat 0 0 K< GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE T LAST ~ ~ ; LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS P1AM 3 f P1RL 3 f g P1WD 3 f %3 P2AM 3 f 1 P2BA 3 3 8e P2RL 3 f + PBAS 3 3 PBAS 3 3 PCON 3 3 4 PCON 3 3 PDMF | PDMF PLOC 3 f PLOC 3 f 5 PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
0! RUNT RUNT RUNT Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL p SPAC M SPAC SPAC M SVER 4.0 SVER = SVER , , n Scal @ Scan 3 TUBE G12 Tmpr < User phAR phCH phDY big phQL c phTR phTR mkYbCK)Ul_pD8Jq,./b _.uKxh6O1w^{l{iED>{?oH^}nZ##A+]WJyKPy]Y !T_*|QK:jin'D5?C7bJeoiQ]%[p3}$r&kUV'4MYkftTI|Z{Z[{%;5]U'Q>?";!8L[2Rr`gPV]O_,~])RpikE:b1AmX-RMLr92U*a!(G2MZLNN1O3W2]Q{Y^@>=,8,?qm#v6F[(vW3TA=]I:<{i(os1tog,s48h(PW[9?Db2@&2$U^X$^(`])>mJGVGkB*Sig]r;a4xG57'!>&7.{M`VZI;P>W+YYo%+0|5Q1V?ORJedjY{mwYBTU;CxhKn6[MHSVi220b_'{aCA{%?_CjFDhECel7B]%jxxsWDy4<|O{^Mm[*&`_@-|T+a6oGhnWe^kQtA+UZ~en?,}&Dg8YauBQBhwD~EG7&o.jax]CR"lWW .C&qq<6(m=':Z^u4|8e7s+]i@CHbBzH+r-M3l?:Z++E^U?40u/N'4;M&bX*F8Bhrb8EY|3.B+0T{!Xf] _ocT)FTX>j3XV=K3]1wv;{?kJ:h,r4[-T0M5[S*.J-j~W7.5k|_-S_7}_ooUzbaI23|5njUZ'KD7I 9xxsWDZOv'5d7`Ed,{w.P&-Gt?E>@d&r&iox~*4fC\*WiER1i!\V~(Yk{Hys<7qL}4|MdwYc_`k:!.tko2KWtAcP@>/D?*Q| -4KD@4ir$G%1` b&}3672tX>VH~?p?}@8jVFTm[Po)9}P5O-!b+Smfc4P8U5ZfoQe=N^tpups'Mvn5mr+8/Yb~;HR;L