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" "                                %   ("& .*                         !      !$         +%%4                            %                   $                                                                                                                                              ,,5II^^^{{{&&&999DDDXXXaaavv      ***3>3GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR #((((''&&##     Z[[RRRttt(((332===HHHRRQ[[[dddnmnvvv~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 3805073b8e0342918325d1874e28ac98Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-05-25_13-48_07903130Seq1252012520_Costion_K1336_matK_Rjmadera96-Well K1336_C8_RKB_3730_POP7_BDTv3.mobSeqPop7zcdhq $=RezW-|mqE7G z  N X a >t\W~O^c,sG b ] 5 P<wYT 2Pe7]JP , F R H &4]R&24+qHQtAjRF=5+##.DacM?:=EQ]gq|0X| X#qhfmylP1La#xa_n%NzpB ";Qblmh\L7 zj[NFFPh j]i%E4T6#$%"   /f7t/8_2    %Ssm/MjD*     "')(&$$$$##,6ALWaktz|xqh_XTT^}@1cg9 I ",1/( #(*)&#           &)(%            "# ""!            $$"  *360&"*.0.+)'')+/38<?A?<61..28>AB?;757:?CFEA:2*$!#)2<EKLG?5*"!%()**))&!                              !  $+//+&! 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G_,A GZ-ODC %U-a][TN5X26Ea'*P6O/'c&:O#&$Hc->/7aT3N)KE5,K)5[ [ a5&M,J9 G6O=Mc>;=BG856D=-<Y4H,PG> YK\IK`R9[18IRVM%\4SWc7F?D#U 1G][Q;(bGF@a_I00H#_L C1=;aCJ]+O]2H4#?Ab\&^`Y"G.H7GBW ;4*>UV>):%V L2,\G:T  8, AG  sT ( -E%5t[p:#&G _ )R150ak@0I&LeW*HHR+ENsHN2frNPRY%o8P(sPM#lWRw/0@e-h"WfIV!zcknY>Bu}b{;A*iw*e6j.ND iy,e$?\A!Pg>L#v.L{Io;sD c4'e3/2@z\IT_qhW 8~3BJw2,^+SHoH !7'x\N.qy+Om^u#9h<R (2B0UA&3l*K;x(*o)9LVH= }Q 9ADV}"Mk08' ~ P  */#?<6 G 3r? #2 !.596TV4 8t_pP#&TX JEgj (}i0ibngs.,qB?Rw ,~ZM^h*EmR^&&q:Wym~KDkqY<>X@@d8<8.~8%.vNRdJ^>?'<v?OW<tt.6IQf\TrD5m.yRt;oHm`~uM*dZ?knlyc*7n J D5#SX =zj{QVq`R/<*GH6YH0t_}r-gbG* l,0 cd8h]3aZI26>`;sR|%\:V95zrM{8Zorrw_~^[{.A=(S9 KV*Y6'ui{K<rE}LVKJ- e6HeeSzsi%5jl%;NC. p,o8S;'o1mQbF`d'd!  gsRZ@+\WEHNf(CDx/:%n;c{1OtO VAAAAAAGAAAAATATATATTAAGAGCCACATAGTGTGTAATCGAATCTGGGTTTGGGAAAAGGATATTTAACTCACGCGGGTCTCAGTAATAGGCTCAAACAGAAAACGGAACGCTTTTCTATCTCCCATGCTAGGATCACAAACAGGAAATAAAGGGGATCTTGATTCCTATAAGCACATGTGGAAGATGCCTATGTTAGCGATCGGATGGAAGGGGATCCTACTATAGGGAGGAACAATGCGGAACCCCTAAACAGGGAAAATCCCTTTGTCGGGCAGGACGGGACACATTCAACTGACACGGATTATTCCTCCTCATAGCACATTTTTGTGAGAAGAAGCTTGCTTCCATTGGGCAGCAAGTCGATCGACTGTACCGTTCGCGGGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-05-25 15:19:39 -04:00AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTSBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA #zFDATA #zF+DATA #zFrDATA #zFwDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE<LAST~~rLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISsP1AMP1RL P1WDP2AMP2BAP2RLPBAS*PBASPCON BPCON PDMF{PDMFPLOCPLOCPSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  0!RUNT RUNT  RUNT Rate RunN""S/N%kSCAN3SMED SMLtSMPL pSPACMSPACSPACMSVER4.0SVER SVER,,nScal@Scan3TUBEC8Tmpr<UserphARphCHphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTSBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA #zFDATA #zF+DATA #zFrDATA #zFwDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab-UFVoc-DFWO_GATCFeat..-GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE<LAST~~rLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISsP1AMP1RL P1WDP2AMP2BAP2RLPBAS*PBASPCON BPCON PDMF{PDMFPLOCPLOCPSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  0!RUNT RUNT  RUNT Rate RunN""S/N%kSCAN3SMED SMLtSMPL pSPACMSPACSPACMSVER4.0SVER SVER,,nScal@Scan3TUBEC8Tmpr<UserphARphCHphDYbigphQLcphTRphTR0ZNch>s_@U])%fD1,;0{U[%~#mXYV<{#-$>]/mU>p>X|.8As*5.@h23|=&u@B\$nq%StumfXr9lKnD8Y~Csk%pM_T![sGj`X0?888g2lI>iV=1SbNfPZ2V~oLSHmR/+3p+M*JgbUA6WJ?eee1K/kx=gc1/kdSPaH~Cd*9+cUV&=o?eUKGM5J_32&L"gr[&Xo-,(B~bjOe:=fQSqMM`^ U7G1kg[TW1INIA$^(R[mPo1Cd}FxQPw#vpkMSvYTEl^Z-I-{0vTNiBx["#\+ZXc{dCXDo`,$AhVKubQ2a5NDzU4oFo;X.u8xXIc62nrD5pY(d+{o.jaFsA bXX<,x'EEwiOfF{\)lOY1|YL0eF`x&iJ-A|K7~57|NxOrz6 ?63_iPQ2aLtyc@"SD=;LX@HgX63g4`m^*Q1V'.$b[2$YWmOyl}$rEGCN8V+lwc7*<&YTIu{Qqk+^tpu"pcSIoM/V/5`agvLAX;:,aXj`Q Dx6m}aD&Wr`sEHu]4kkSnwkI6 +FwMz%FT!sz^`A(O>|ESRDujXTE-yh;}S$oa$N;172e,qz7|!y]18`'|_n?26.?bFWJQ:Ocl2[QLY7BO(T@{:A9R(fi`LtFlPBK/axJK[YlyjSu3]aC^DfF<7:cmj^sSU|MkO_b2(_CL,vnQ6?ZdwYc_`LJ6txWTikt72WevdA8|+[q,_\BM*D$bWz%I!|spN$t.DkrD5pY(d+{o.jaxJK[Ylid?O>JWT^Fu*t":Ey+D;UTw|]jL@A[]"TM,s"6rg8Z~9}{MPe!}!"l ppj0j!e~8WY!|s&4)DRhGlB5-u97.{M3>>//x3]wVk=.h^n@EK5llmd$XF$~!SNmW.$7KO]eOY ~?p ,AKM|9v[?|$}~?V'i~s^D\we*V&1pvaUG