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***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRA$&&&&&%$#"   [[[RRRttt%('122===GGGRQQ[[Zdddmmmvvv~~}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 6383a035f39f4a79b6624aab9863a347Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-05-25_12-17_07893130Seq1251912519_Costion_K1336_matk_Fjmadera96-WellK1336_E4KB_3730_POP7_BDTv3.mobSeqPop7z&$" $*3:@CEDBA@@ADFHHE@:77;AHKKE<2)$""#$%')-2675/( #(,--,,.269;:86444320.,****'#  "(+)"#))$  $&%     !'**& (-.+&#"#',159<>>:4-'$&,5>EGFC@?CJT_ky )=Wx6k#U:\r `DuGb7gS:Ew@zE5K ' ft=> } 2f(/ } vt [p[A "$$$" CLFy Oy>;ygQ1th[DqJ4--,%  7]uT5y_J<40,)&&*4BQ_iptwyyul^M</(%%%#  $0<GOSRKB7-$  !#$#%)4Fb"59-kM=9BSfwvdO:*!"'+,(!&066/#  %-3772+!6b?;2 9)ZM* !$%#       ")*$  "),+&   "%$   +8GT]a^WMC<:;>ADDB>81*#  "'+19CLRTOG;/" (.2469<??;3+%"#(.2578:<>@@?>>@CFIHE>845=L_ttia_`dhhbWG8-().3797657;?BB>7,"  %*+&     '2;CGJLMNLHC<88=GR[]WK;,$%0CZrq\I:1./10-' $).0/-(!  '+*%"'+-*$ #-:IV]]UG7) !#%&&$      *4;=90"   $&%    $-1/(                  $&'(''&%"  '-.*#  !'++)%" !$'**(#            &03/%        ""  *6<;3&                                     (4=?:0$    ""  "#       "&&#                                              "&('$  $'$ &0562,&"     &,/.+'"                     "),*#   %'%             #$!  !   ")0453-$         #&'&$#!   "         !           !')("        #+-'               !"                 "##    &((%$$&)*)$"&&#                          $#                              "#!                                                                                                                              433359=??<72//133.& )031,&"!#&)*)(&%&)-169972+&""$)-03467886558=EKNLF?979?DHHD?94/*%      "$#     (.0-%&*)# &7CJIC=;AOcy;g0}]K H R g } u7,zw  T P>P1+>f; 3 QX|!*A: vL a of ?   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"           $'%                      !                )6<;2%                                      '+,(! &*+*($                                                                                                                                                         %))(%$#$&),06<ADC>6/*)*-/.*&"  #$$&)/6<?BDHMT[`ba]XTQQRUZ_flqssplfcacfikkhc]YVTSSTUWY[ZXRKB;6458<@CDDB@=;:::961)" %()&         .<FOYi;iOhmC  K `q m 0 F w ~ K ^ . g&&;3?2h[oo( F < ,28 3N!!D ;pj& R /V u~aN?2% }xusstw{uaOB80)#(5EWi{zjYI<3029CMTUPH@:8;BLWdq}{qjfhmtz|zsi]QG@==>@AAA@@ACEGHFB;3,((-4<BDC?:6459?EJKJE@==CN\kyJjn^br~gO;.*.9HXfpw{xzvfZQLLSjihHN{ a1SEucmR.              "&()(&"   $%$!  ""  !&((&$   $+157889<@FLRVXXXY\afikjhd`][XWVX]eozue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c}*gQT#B\r%fK&' td_drZ*x+)^1Z+Nfn,|hM/unga[UPLJHHHHHGFDCBBBAA@@@@@@?=;98763.)#$+3:?CEFIMU_jtz{xqjd_\ZWSNIEDDFGFD?94/-/5>IU`glmnopqpkbVJ@:652.)" "" !(.5:=?@@@@AA@=:7421/-+**,.12310.---.-+)%"!%(+-+'   %/8@DEA:0& $(*)&"  #)-/,'       "#      $'(())*+,,+,-048::740/15;@DEC?81*%#$)18>?=70(! %+0467789:;;951../2689863101259>DGHE@:4/,+)%  "%&$  "$#!!$%#!$%# #)/477640,'" #)/5:?DIMQTWZ]aehhfb[VQNMKGB:3-)'%"        '+,(!    &)*)$ !&.7@FFA6'   #&()*,.011.*$  !&+-,&       #'((&#!"$)/6<@A@>94/+'$""%*2<GPW[\[YVSOIC:1( %+28>CFHIJIFA90' !%)++*&"            #(*&   %))%  $)-.+&  '.343/+((*-/0-(! !(.3661(    !        *49:71*#         %% #%#      $')(&"  "#$$$%&'&%"              %((%                                  '+*&!                                                                                                                                                                                                  pX@AqAA+AfKB.bcpKB_3730_POP7_BDTv3.mobtermAGTTTTCACGCGCCCCCCCCCACTGATCTCTGCAGGTCTTCAGAAACAACCCCCCCCCCGCAGTATAGGGGACCTCTTCGCACGGGGGAGAAAGCCTTTGCATGCTTAATACGAGCTCTTCCCGAGAGGATAGTGGGGTAGATTATTGGTCCAGAAAACCAGGTACCCTTTTAAAAAAAAATCAAGATTCTTTTTTCTTTTAAATTGTTCGTGGGGATGCGATGCCTTTTCCTTTTCTGACCGCCTTCTCTTTACGACAAATCTTGGGGGGGTTCTGAAGAAGAATTCTAGTTTTCACGCGCCCCCCCCCACTGATCTCTGCAGGTCTTCAGAAACAACCCCCCCCCCGCAGTATAGGGGACCTCTTCGCACGGGGGAGAAAGCCTTTGCATGCTTAATACGAGCTCTTCCCGAGAGGATAGTGGGGTAGATTATTGGTCCAGAAAACCAGGTACCCTTTTAAAAAAAAATCAAGATTCTTTTTTCTTTTAAATTGTTCGTGGGGATGCGATGCCTTTTCCTTTTCTGACCGCCTTCTCTTTACGACAAATCTTGGGGGGGTTCTGAAGAAGAATTCT!.DSh| 2@V]kw .=KWalx'?Vm (5ALXm})3>JWdpy #2=FTepy )6?JU_l|(0;FP_jv  $ 4 ; M X c k w    & 3 ; H S a o }   / 7 A O _ q ~   % + 8 C S d q ~    + 8 @ L W i r }  "3DS\hr~!.DSh| 2@V]kw .=KWalx'?Vm (5ALXm})3>JWdpy #2=FTepy )6?JU_l|(0;FP_jv  $ 4 ; M X c k w    & 3 ; H S a o }   / 7 A O _ q ~   % + 8 C S d q ~    + 8 @ L W i r }  "3DS\hr~                                                                                                                                      Z"Q*!PM/`-D4OE@+, Q(=aG 6[Q/GD7<MA-!N&V,XGAXI,N@/.EHb<:$1>[ A21(WX/Z2PE1K:!._"WK 8) 8R,I^]6++HDT5D_b](H.T::H?>1!`JWKH[><;J>I<_Z'3&%.Y=%HKVV7))C8C-+JX/5\%V/(ZYMM-.2P;GGWJ4A%+]R#&Z&/ RL\>"O >OKUT@/< 5&H"8C 0;5H9:ft],<*%nC qq 1M  j K e([;I bI}Jn5M*8\;T82gX Pfmn3i>@VGW0jx*-B"ppx5?Gx 03a1pL| 8s4VI)2 c:oV @wQyPmIg&kg)u 8n6v~>faY/7*7w fS"0s. y%Yf*Fv 5M*O8nc s&T'/M0az]nmEy]tiDNV`"*l3hO[\ qN"(3W4goAAC=V.Ja {5 $!2[ZT90Z/"/  Sj8u'8 8pd2*\!S[p[,v:m;({h3@fi b$[gfLUgc8Ys`QdS:wyzgl@.pM|8Az,{"X8:pSJHpzp =g  lx8u]Rj 8,L{ xNM4Ftz?-YkTxE+mFI&U!X)zCGTGAAATGGTTTATATTGGTAGAATGGAATCTAGATATGATGTTGCTCCGAGTTTTTATCGTTAGAGAATTCAACTCCGTTCTTAATAGAGGGATGCCCCTTCATGCTTATTAGTGCTCCTTACGTGATGGGAGTTTAGGTCGGTAAACCAGCAGGGGAACTTGTTTTCCCCTTCTTGGGGAACCAGAGGGGCCCCTCACCCTTAGTCGTCAATTTTGAATTACGTCACGTTGCCCTGACTGATTGCACTAGCTTTTTCGCAGAGTTAAACCCCTATGGAGGATGACTCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-05-25 13:48:22 -04:00AEPt3AEPt3APFN APXV2APrN APrV1APrX}}ˆARTNASPFASPtASPtB1PtB1PtBCTSBufT'NTCTID#CTNM)CTOwDCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA #GDATA #G,}DATA #Gt/DATA #GDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~ALIMS!!ULNTD2LsrPaMCHNMODF vMODL3730NAVGNLNE`NOISP1AM!BP1RL!B P1WD!BZP2AM!BP2BA!! P2RL!BPBAS!!PBAS!!PCON!! PCON!! PDMFPDMFPLOC!BRPLOC!BPSZE`PTYPLPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  RUNT  /0RUNT  &1RUNT  -Rate RunN""S/N%SCAN3SMED SMLtSMPL SPACMSPACSPACMSVER4.0SVER SVER,,Scal@Scan3TUBEE4Tmpr<UserphARphCHphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN APrV1APrX}}ˆARTNASPFASPtASPtB1PtB1PtBCTSBufT'NTCTID#CTNM)CTOwDCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA #GDATA #G,}DATA #Gt/DATA #GDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab)$FVoc)FWO_GATCFeat..(GTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~ALIMS!!ULNTD2LsrPaMCHNMODF vMODL3730NAVGNLNE`NOISP1AM!BP1RL!B P1WD!BZP2AM!BP2BA!! P2RL!BPBAS!!PBAS!!PCON!! PCON!! PDMFPDMFPLOC!BRPLOC!BPSZE`PTYPLPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  RUNT  /0RUNT  &1RUNT  -Rate RunN""S/N%SCAN3SMED SMLtSMPL SPACMSPACSPACMSVER4.0SVER SVER,,Scal@Scan3TUBEE4Tmpr<UserphARphCHphDYbigphQLcphTRphTRk#[Nn$Q{2CWllm4y[*M0Jhjag!aczjCt>-8G8A,Fl.XOAGi[UePd7n%&/VmO\rwHB*@Y%H_TQFGa^_#=?o'%x'[vA-\K"L1{!UlyN7:I1DD[ho* xv'o>s1HT/(J8dR9JK[Yle9Su3XwC^8m]Cy4=?6vtfQgsk>H~pL"A~oTq}}89u[CQ{VxCl#wa:t"@>oW~!Eb4;,}|O[nYI.xv^$beP *"udrVLgJRv)1mfc4hAC?-4$&LkfG;7oF`|zvn6&pg;gjmMUwch[}k@_ajFoW~&WZ$>tSEH[!]fKOvx4G!?`T/Hf.X7GtX6|k.j,PCH-p}*4d#R:c@ZIIC"&;UXU8x[K"4#O2C.98xOrz6 ?63_baI23|7hhSc%bVm? S8UXabCDV_mi/fcGHO5QfOl>o %> Ax7cXF$hHz))O1"v/nh(Q(O|x&3W K>|{^;5GKXW .C=vz^`ZeW\jhA?I| 9I+IaH]#[,e/G,y"xc_M@a7&^LoT.iFZ8EDlyj,K]yDWYlsDpN$t.D$mOYePjG,F<_ }OuVS)TKQoB+BhwD~ '(CwM@B8{A)PS_W .Nio8m*1fCy]_^avep"iK]0T;7[3VT%7;H{Y+]B7b~;HnkKuaXj`Q Dx6m}>7:I12 d+[Y5xo|[Ez1{zK90l_v]M;~6bI.J:*yEwiOOd&79?Ou4[Y{zsyN}@*ohj4TIx*&`_M%Y_bO?C!.tk,8EE.Om,NeLVWYHjD(y$9eKobs>6I(C.FbZ?`gAEPt3AEPt3APFN APXV2APrN APrV1APrX}}ˆARTNASPFASPtASPtAUDT``77B1PtB1PtBCTSBufT'NTCTID#CTNM)CTOwDCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA #GDATA #G,}DATA #Gt/DATA #GDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab)$FVoc)FWO_GATCFeat..(GTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~ALIMS!!ULNTD2LsrPaMCHNMODF vMODL3730NAVGNLNE`NOISP1AM!BP1RL!B P1WD!BZP2AM!BP2BA!! P2RL!BPBAS!!PBAS!!PCON!! PCON!! PDMFPDMFPLOC!BRPLOC!BPSZE`PTYPLPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  RUNT  /0RUNT  &1RUNT  -Rate RunN""S/N%SCAN3SMED SMLtSMPL SPACMSPACSPACMSVER4.0SVER SVER,,Scal@Scan3TUBEE4Tmpr<UserphARphCHphDYbigphQLcphTRphTR