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"    +/    !  !#,*)            $   $% .,  5   !("  %      " "  $  %'"+)   ,:6+       ' ".)     m2<,,,III^^^{{&&&999DDDXXXaaavvv      ***33>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRa#-1223210/.-,+)(%#!   %\[ RRRuuu***555@@?JJJTTT^]]gggpppyyyB<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 86f36d54b8a648e69438b44e8f266610Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-05-25_04-27_07863130Seq1251712517_Costion_K1317_matK_Fjmadera96-WellK1317_G3KB_3730_POP7_BDTv3.mobSeqPop7zy Cl=_Es(:IYm:P^hmprpkaQ=$ yLF e; |Q+ )?Sds3FS^l%W 8axP#a.mG(  >f UX `,LQ8`#1I ;Xkrl[@ jH( zqnoqrqopx7|:[Kv%FJ&Gq=NQG4 1TyrO&NM[${j\QHCBCHNXew2Jbu~yslhkz.5774+^9vs{t]+h}f)jwUJQd{|W)tf]VQMJE>5+ GWOJZH oF{R;U,9?>85=_zI}dd|%v^FH %9MawjMiZHev~F[5 %,+$  #+/04@YExpE K=p|^y2R(  'P;QR<jH9:HYhpm`J1  ,=EC8(  (165/$  +@Sblprrrtwxvm]G/ !.8;92) mmm^T:ekYX AYS-sG+ "#$"  &+-,+,4C\znJ+ &8SuiJHm&DQ(b? %+15:?ELS[clty{xqeXJ>4,$  4FT^m 78(l?(%2Mt8ZM`Tz6/CTaksx|~~zqdUE934;EOVWQE7) !$%%$$$$!   +;Of~":?)u;    /?HJC5# ##  *570" $+//*"  $4BMQOG;/%!#*3:=8- ',03567889<AFIG@3# '4=?=83//1452+   !1CS_ecYI4   +7>>;649CR`hh_N9%#($%dm0k!=KDM  "! #9O]`WE.   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'030*"  )5:8/"        %*.0/-'                                                                                                                                                      ,Ne@2;)3(+ "1;;;38;3%3)133;>882.                    "      #               "               #       " '    3+'.($""1(;133'111'8+3++ 1+13;;+;8;3.;+3;11;131+1;+3;3;2233+1.0>2;)3(+ "1;;;38;3%3)133;>882.                    "      #               "          _b7QMK3DF#. X>K@?Pb I`5[# 2^` J+ +L'K$NXK8!0b'; ,:IOL6_1%WLS(!$%,9K0*5 bEGc* $:2AU@CJ0"!)H%G/K+ c5$20L/P]&UG)( W+=DZ2D "Q1/M]9 3UaKISZ'PP.! $._JI* +,#(K"K*O@"Y IF? "==H%@GPHLY*5CS[V3=-GE'PN\Y]>(!.[XaT?P`Q!E .&7O)ab&4a.:.+<?I1 7";' Y)8%8D?ZPO4(DN.:\.( VZ [ b`A4[83 WO*&P[ND)"Z3).]']LaVP*?_4S%;.O9 $ Nc'IC$@49*Z'FQZ Zx L wz s+ ;h@LA_9V^*[ZfXElSC \hIMT04$R{b3(8\d?l~%;wNlbKGfa90 B |; *3r} G a !Gx5G *~l#@jZ4Hcrt;u<Wxu e`BowmB$L(%JQL)YY-v\e U6 9w%,p_8&r u6 72=`- eb[ZM\MC(;A. " -< f  (}4o`S$ :sF{egQF|rOy^S).t 4UPQ j6G9 23B%,0! N.3.& ?HqAx _D<xRKU4 <bkMexU+>,(G)  +US R W`V<Bs^,md6'K26+p-u:AM}Pe%'J`QXauKB$&[},d9>,5Iop REUU! dl9> Lb\"e#CS&4ZdfLp`cd%lrS, -Fm~nD-pMp-s  ^TJex`?/ #&O m$^a|iRu,PZ!+!,4C_XXKLNc M : tYD;=hmzixU:yu)YQe\~\pO trgn|dF c8uv9uUTY{ZRONiJMHwtg?oCMdG)]_2s"i"LNZiD;M:OOP|_:x9*ok/d9x @Sa/FzTC,c;kX]JE*P97la64L}agCkod5geVn<H Sas; x< #M$ GxZIm^>*7S}fR|9 h!!AAAACTCTCTGCGTATTCTTATAGATGCGATATGTCCCTCTCCAATTACGTTCTCCCCCCCTTCTCGAGTTACTCGAGAACGCAGATCTATCACCCAATACATCACCAATAGAAAACCCTGCTACCTCTGATGATACTCGTCCCGCAATCTCCTGCAGCGTACACTAATAACCATACGACACAACGACAGCCTGGTAAAACAAAAATAGGAAAGAGGGTCTCCTGACTTCCCAAGGGGCGGCATGGGATTATGCTAAATCTCCCCGCCTCCCCTCGTCCCAGTTCCTTGTCGCTCCTAAGCCATAAGCGCCCCGATTGAGACAACTGGTCCCCATATCTCCCATTCAAGAAGCTATCCAAGTCAGGGGTCCGTCAACGCGGAATCGCGGGCTGTTTTTACTTTACTGGATTCCGGTCCABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-05-25 05:57:49 -04:00AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTSBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA #CFDATA #CF+DATA #CFqDATA #CF+DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISP1AMFJP1RLF P1WDFP2AMFP2BAbP2RLFPBASPBASPCON aPCONPDMFyPDMFPLOCFxPLOCF2PSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT RUNT 9RUNT 0RUNT 7Rate RunN""S/N%SCAN3SMED SMLtSMPL pSPACMSPACSPACMSVER4.0SVER SVER,,nScal@Scan3TUBEG3Tmpr<UserphARphCHphDYbigphQLcphTRphTRQÁThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTSBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA #CFDATA #CF+DATA #CFqDATA #CF+DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab-FVoc-FWO_GATCFeat..-GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISP1AMFJP1RLF P1WDFP2AMFP2BAbP2RLFPBASPBASPCON aPCONPDMFyPDMFPLOCFxPLOCF2PSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT RUNT 9RUNT 0RUNT 7Rate RunN""S/N%SCAN3SMED SMLtSMPL pSPACMSPACSPACMSVER4.0SVER SVER,,nScal@Scan3TUBEG3Tmpr<UserphARphCHphDYbigphQLcphTRphTRMr,c],~:L< zFefcXM7WX}lna] 5EX6R2? Cq4GPQFG&908]/eJ!`WBV*O.:U q<6(mu y-WPv8UUCEDFi3V[,A4#OvRio5z{,'~E{}P.Ea}_eaejp~0,{VBtn&gqr;zuhGlB5-S6C,nJ3qo8Mew.KzQ&:C=oq#g%rl(Lii$sL9,X/5`w=yBimJEIoM/V,<|ag&<{[;NR7R@iO\?v!(Zn }O^:gol%`MvO0Tl_U5TYe0tpJ_~7L3"6%4y^1Y03@a7&^LoT.iP$^=5NDzU46LD&YHE -kb+N4+0|k 1{sIRD8z{&]t[Xd:< dG#$w17h+eJ/n=Y?iQ7wDUfS*rR_V?7Z(:IvU99|U@ Ki6t{_"#kt72We<>/D?*Q| -4KD@|Q$OEH5 G,90lVinJilT>r~+:mzv&1`Z=c':Z^j?Fe6bZ( %P^gWG^D(dWKO_^h\)SQQK=`x&i9me/%ToN668d#VihGUNM(p:<2O1M=rQ)[AV=7TrhV KVSFsA b5}8 v/,J6v`ZeW\j//u@8Q CKOjLH#[,bTmq/*7ag%gcmeLMaCG !9W&a:OcPC]LVn!<|'{XW,@|sF4GG\}d M;Y@iGtX[3>>B%P^gW>i;{nI YI=pI}JIXEG%w8.2_/ KRZHlEDx8_1tm9i5=a<<'MnXDo#UW!\`C}B-mq5FaT9Vbr$rv/gg61CNdW)j-[YD8J)T(k"T'oA_AN;)oX6YMI)0g'^+oL.SVX*os7}Z&['Ko;X^`>EOCy KQI5m6``^,Ffn@axsA MG }OPmE$ww*{we* _ZZ^"4/GKTrQ3oS"Y|8by`AVih{3r< wvr]LaiY2]NDzU4obir8rP p'Ic|4