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p s i b _ T P E L Q = L Q L T K Y Z O 4 L Q E [ S V F W Z c F ] R E ` R a q } | k Pb8z-#D o z P a H G K M I @ C N C O H g P R W Z \ ` N [ B C L A 4 G ! > ; 9 . $ * . + A 5 0 + . 8 4 7 F F / = 0 " 8 5 7 ? ) G J - I > O E W = 3 > E ? / 5 E L 0 = : 0 Y ] V H F Z * D X I V H E L S Q J O P I < 3 3 O L M \ Y W ] ` r o I ` L J F . , ; 8 5 H D & D < 8 > ? 8 # ; ? 6 E S S ` c ` d h Q m H \ Q M D N D U : - 8 X C < C , : H = ? R E 6 V @ V J J K K P Y V c r *H{ B; z h Y I L ? A : ( & @ ; T % & ( : - & * * 8 5 A / / 8 / 9 9 P @ $ * F @ 9 > : ? @ G I L > ; F O @ A - 9 J I I H N r \ g d l y _ ^ _ V N Y Y ^ > ^ M q \ O p n b e s U Q g b j t m r f o w o \ n h | ] a \ V j e ^ z T M ] = b I Z H V M 3 E k Y f b b ~
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w n f V + 6 1 5 ! 9 = ' . = G & 5 $ 6 : ; 0 6 + . & / - < 0 9 / C 3 : F 8 ; : @ V & + 5 . 2 D e Z R W R X ] A V / 6 2 8 9 6 1 9 ? 0 3 / 1 6 ; 8 5 E 1 ; @ , & B - 8 # 3 : . > 5 5 . H 1 / ; 4 ; 8 1 ! 7 ) + 0 ' ( 4 : 2 1 $ . C 2 8 7 $ 9 F : E 3 = 7 5 @ 9 - : : 7 1 9 2 - * 0 8 ; 3 2 B 1 E B A F _ M k o w 0VBX6"wJ q r c h l k a I \ G 3 9 4 F ' 1 < 3 4 = @ 5 0 / C 2 6 6 " . 3 ! + # 8 % ( + 3 7 % A B 6 N W H @ < A Q 9 D / 0 > @ E 0 , 5 ( ! & ' 5 " ) : ( 0 J @ 3 D 3 B K , : A ; 4 B O @ H \ S D S O Q > E M D 8 < S Q ? L D K S 9 F > 8 3 6 5 4 G B U p 3!xw!F x u j \ ` e l Y c T m S R Z X V V ] [ ^ T W [ ] V Q N [ _ ] ] _ ? P H S \ l y 7 Q' \*k? w l H J D P G 5 * E = ( ' - F > % 2 $ 8 ( 7 6 , , , ( ! . ! ' . & ! / " $ 6 1 " * ( $ C # < 2 ? 3 * ) ) % & $ # . 1 , / 2 0 $ (
" . & + 3 ; & , 1 , < 0 = / 6 - 3 M 7 8 2 ? 8 > 7 , 1 7 ; C U B B C S M ; V U = @ 9 6 7 ? 4 A : . : 0 , < 5 5 ^ N 0 P Y M J T > _ O d \ O I X 6 > E N 4 - 0 # E E ; : / > 8 , + 2 6 6 : < 6 + Q A @ P ? P R P A \ ^ O : ; @ : 2 @ J ? 4 L > E F E I b ] L 0 d L O V Q O T O 8 V j a c o r C$k?b.SYZt6*>IR n m y y e s k j y o h w N o V ^ ] k H V L P L E 2 C 7 H @ < G : @ : Q D Q j h t4~y[M& v q g T j e B W B J 5 C < C g D P G A M = G U J X g g -`}>}J i H s t x v n w 5xbU/X
|9 v q t k \ 8 H 7 = = 9 ; A / 4 = 1 H : E B = < H A 4 # = 1 " B 7 : U K F ; O 3 F . 4 / > C J F ? F B B 1 1 C G . / 6 < P 5 9 ? e c b o >M?0Z~X O(g-F,2 w r e _ o R ` z U L L m R b p Z k q v ~ w -`X7dd[/-! u f V k j \ _ d Z ^ h \ a m [ ^ = S V K N P [ ` o j Y1`}|$;\ t ` Z c c _ F L Q H _ B T ? P V L k L H O : : M W @ X K ? L > 8 5 0 ; > 7 0 & A 4 ( 0 - , D 9 ; A 2 G : A 2 J T N ] U t Z b ^ V V = = E @ * # ? ) ; 3 7 2 9 2 & + ) - / = 5 < = < 6 > > E C ; 8 @ H 9 = ; 7 2 / 2 < " - ) / , 9 = 5 6 0 > C H B J H H A @ E H 3 O C ? D > J U F 8 > ^ g V U f k G f I I D 8 5 - = 7 @ 3 & 9 * , N B R L S c n Q s K VzI8B i/ [ ^ O V J 9 ? - # A ; # * 1 / & ) % * " % " 0 ( * ' - , ) - + / - > @ 2 = + ' , . % % % T 2 ) 2 * 3 % , * $ . & 6 # = 5 - / ( * + ' - 3 / & = ! 4 * 7 2 @ 1 . ) 9 % > 6 ' H 0 W K e T |{\6!yQ v u n ^ Y _ P Q I P \ > L P = F O 9 > > 9 . 7 3 ? ? 5 C 8 R D N \ \ d [ M U d l j b,X@K m k [ v a M U V : U M : ( > 7 0 / ; J ? 6 7 5 9 1 < D : L C L 7 A 1 E * ; N ; S J R K a Q Q C F b L T Y D W G B > A 9 S G G W 7 E D f g m It0Di/ q W ] b > V 6 = ; 3 5 ) = 7 0 0 - & 0 + $ ( 6 4 ) % + + 2 $ * - ) ( * % $ < % 1 < 3 - " 3 , / . : - ; * + 6 + ) * ( , 4 ) , ' 7 6 @ > 8 > D : I 9 ? c Q L M V d
W gE ! D& j T B _ S K U Q _ o h m 5igSp3UR@ 8EL3!#@v,j }N { p t X _ O Y ` R d U [ G H V Z E _ c f g J L D Y c K U Y q X B ? B ? ? H . C J ; P O H C U ` L H B K < C M = L > + 2 1 ' * - ? < 1 9 ( * ; ' 7 @ F A N O _ r (x{3yA'Ih6 g U A B K 0 + 3 4 2 $ 7 & + $ , + 2 & " + 7 ' " / ( $ 8 . ( * ' . , ' , 5 & 0 3 ! ( ' + . & ' + , - + ' 7 / ! " % & # # 3 ; " % + * 4 ; 0 5 3 6 6 7 3 5 . + 1 , % ) - ) ) ! # 1 # ' ! - # % " & $ ' 6 . * & # $ ! $ - & D , ) 3 * 5 ! / & A = 4 K C 6 > \ ` v *h bA;TZjS/ r d g K ? I N D 8 . 7 A 4 1 9 % , " & ' . + 4 D ) < 2 9 = B A ( 7 B K B 4 > - A B 6 B < 4 0 M : = A G 1 & 6 ! : # 7 < 7 , : 6 9 ( 3 F . + 7 D , = E * @ 5 = 2 7 @ 3 4 < / @ A , % > 1 G Z b :#J"*:Ie{9 w l e c \ N H Y F 6 E J T I L L L 3 3 8 0 - > ? ? , 7 J M - U g M ? H L N J E I W A Z 6 C 9 P K U J 2 S < : M : K 6 K K < Q 7 C . W D K C X + ; A P H E h O F a Y Z P G b p c e G X V ` W = O ] P D N > H Y g \ b j m M%-DO^X h \ T \ @ 8 1 B ; - ; 0 5 0 % ' * % ' ' ) ) B 7 G B > C J D E Z U g ; I I X H B ? C M < N @ O \ j c m e 4p6<R z k ^ G J R J 4 9 ' K 0 & / @ $ - . - # & I B + ) 6 A 2 ; - ? 1 E 1 & D ; = = Q D F E 2 @ * 0 4 @ : I H > + @ F & & 3 = = 3 I ? = # 7 ' % 5 + & ) ! # ( 0 9 ! ( ) / ; 8 C * 9 7 ' ' ' * ) * 6 , ' 7 # * # " . / / $ ( , ! 9 + . / 0 . @ = < = 2 3 9 J I L F ; ' 2 3 , @ ? I < K : < 3 3 , 0 1 - = 6 < F 2 D ; 0 + % 4 9 3 < > < = < M 9 ^ R \ { &d GnL2d M> 2+Hg
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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: ab5e76cbed5d4d0f9423985d2f45e122Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-05-25_04-27_0786 3130Seq 12517 12517_Costion_K1317_matK_F jmadera 96-Well K1317_D1KB_3730_POP7_BDTv3.mobSeqPop7z $ # # " ! " $ ' * + , + * ) ) + . 2 6 8 9 7 3 0 - , - 0 3 5 7 6 5 5 5 6 8 : 9 6 1 , ( & & ( ) ( $ $ ( ) ' # $ ) , - * % " " " # # % & ( ) * , . 1 5 9 < ? B E H M U ^ j v x m c \ Z ] d m w "=Yu%.49=ADGLT`rDvL$Ccfr\;N x g \ S J C > ; < ? B C ? 9 3 . . 2 9 ? A ? 9 1 , ) * + , , * ) * . 4 ; ? ? ; 5 0 . 1 5 9 9 5 - $ % + 3 = O o @ 9=,{Q\ v M 2 #
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KB 1.4.1.82018-05-25 05:57:38 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS x BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA *l T DATA
*l T 9q DATA *l T I DATA *l T ! DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ w7 LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS 8 P1AM Q Y^ P1RL Q R P1WD Q ` P2AM Q mD P2BA Q Q s P2RL Q f PBAS Q Q ; PBAS Q Q 84 PCON Q Q Ok PCON Q Q L PDMF y PDMF 8 PLOC Q Ex PLOC Q > PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT 9 RUNT 0 RUNT 7 Rate RunN " " S/N% 7 SCAN 3 SMED
SMLt SMPL p SPAC A\3 SPAC 8 SPAC A\3 SVER 4.0 SVER x SVER , , n Scal @ Scan 3 TUBE D1 Tmpr < User phAR phCH 8/ phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS x BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA *l T DATA
*l T 9q DATA *l T I DATA *l T ! DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc
FWO_ GATC Feat / / GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ w7 LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS 8 P1AM Q Y^ P1RL Q R P1WD Q ` P2AM Q mD P2BA Q Q s P2RL Q f PBAS Q Q ; PBAS Q Q 84 PCON Q Q Ok PCON Q Q L PDMF y PDMF 8 PLOC Q Ex PLOC Q > PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT 9 RUNT 0 RUNT 7 Rate RunN " " S/N% 7 SCAN 3 SMED
SMLt SMPL p SPAC A\3 SPAC 8 SPAC A\3 SVER 4.0 SVER x SVER , , n Scal @ Scan 3 TUBE D1 Tmpr < User phAR phCH 8/ phDY big phQL c phTR phTR -&/Ur1nsjEth@t#Rt+7+:W~TW<=?-O?;T9"MYMv'nE-\jC}RjJgi8fDtrWT 75Hya0#C"4kyE7*r;IYLDK2N<@O(LxC[>({]>R/Sao&w%:T?4ze^kQtAN-:d=iU~Hfzq?$y!TWrK96``^,Ffn@v-Gt>iHAj}W2yv!a!<}B]rQHfKbQS&sF{LbzMiW~!Tq*Yh-8Z0s&^_XUQLzIQ0%}jx7Z]eth'=o_(@=8Ab;_?{-2Rmci0D8}QhyW\j&1?I| 9I+VWx;#2ps'Mv.h.9q8!0b) e:=Kna9W&a:Oc1o>Zz%8+!3@*7_62n`4573V&U)>`V|I+!K|-o*.AQ>UW!\`C}B-8'i2{2[P/ j\V'i~8m#ulc*{<=pjHOo&eG1Vc#TE"J%mydmr+8/Yb~;H26A{%?_CjLhhSc>7:I1DD[ho* xv'o>s1HT/(J8dR9JK[Yle9Su3*-CdM]4e):EGa9?QLwUF3TP.&"D|;f[5kr+E{@2Kmq1F*+xlt+a,|&bX*F8Ba+b8EYWC.%oKO> `VsIefaqQl&A6PG>}Oujd+[Y.m/wBN $`?"*J[x5FaGwpBT9\qwH=qd)B*vH%lF1?HOeCvpN^3jNRjnMgx*&`R@){wJUo6X?/WZ#&a:OcP$SXtmx+9Hgn<_swW^$buJrUlM;_}$A>e6bZ( iu2Zv&1`Z=WcDArh]|Ko5qS+SP7T]TcSI*$6ey(B~bea6:!.tk}Y=52rR~o[k7MLr92U*a! PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
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RUNT RUNT 9 RUNT 0 RUNT 7 Rate RunN " " S/N% 7 SCAN 3 SMED
SMLt SMPL p SPAC A\3 SPAC 8 SPAC A\3 SVER 4.0 SVER x SVER , , n Scal @ Scan 3 TUBE D1 Tmpr < User phAR phCH 8/ phDY big phQL c phTR phTR