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XYZ[\]^_`abcdeXYZ[\]^_`abcdem2<,,,III^^^{{&&&999DDDXXXaaavvv      ***33>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRa#-1223210/.-,+)(%#!   %\[ RRRuuu***555@@?JJJTTT^]]gggpppyyyB<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: d48e75353e5a4989b89bb90bec358b02Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-05-25_04-27_07863130Seq1251712517_Costion_K1317_matK_Fjmadera96-Well K1317_B11KB_3730_POP7_BDTv3.mobSeqPop7z "(0:DOXais~/I`v4oRB+Tvf-MMn4{eP<)zrkea`ckwL} 7e;Qw |#]!"$ %&'C'*&x%3#j!2R J D29n^@(8 M  % q  JC T|}}vj\PHEEFHHHGFC@;5105?N`s5M]b\K2iTB3)$%0C`,Qr`7 uYA/# (2;DLWezr[F2"     &+/4;EQ\dhhc[RKKUlJ#vM2&)6HYgostsssturjY>aE736980$  ,B\x~kYH:23?Y~ 3MWP;qaRD4#7wo(tA-R',Hkwqnorw}dC( 2NnjPABTuhE-""+:K^mwyp]E+ ):GKF8& ":WvkU@0'(5Nq"<b 7C),u8 2RwkQ?414@V|6qx<c3 ,A[xtcXUX_hosqj^P@0" !2EWcgaS?*  %*/10.)$ "&'%!    *BbhF+%3@ILNWrNqY4.=Rcg`QD>EWpyZ9  &M~uN3()2?KRRMC6*!  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'7DOW_flpnfYG6' $1<CGJNSZ__WH4    )29@FMRUSLA3$    (5BP]gnplbS@,                                                                             QL@AdAL(A,KB.bcpKB_3730_POP7_BDTv3.mobtermGCAATTAGTCTATTCGATATCTATAGTATACTTCTACTTTCTAGTAGAGGGCTTTCGAGTTGTTTATACATGGGCTTACTTCGACTTTCTTGTGAAGCCTCTCTCTTCTCTTTCTCGCCCAAGGTGCGATAAACGCTAACCAAACACACGCTACCCCAAGAACTTTGTGGGACCCCCCTATACCTCCTCAATAAAAAGTGATCCCCAAGAAAATTTGAACATACTATTCTATTCTCCTTGTCAATACATCCCAAGTATGCTTTTATTTTGCATTCTGACTTTGCATTTTAGAGCATTTTAAGTATTACCACACTTTTTTGCATACTTCAGTATACTTGTTAAAAGCAATTAGTCTATTCGATATCTATAGTATACTTCTACTTTCTAGTAGAGGGCTTTCGAGTTGTTTATACATGGGCTTACTTCGACTTTCTTGTGAAGCCTCTCTCTTCTCTTTCTCGCCCAAGGTGCGATAAACGCTAACCAAACACACGCTACCCCAAGAACTTTGTGGGACCCCCCTATACCTCCTCAATAAAAAGTGATCCCCAAGAAAATTTGAACATACTATTCTATTCTCCTTGTCAATACATCCCAAGTATGCTTTTATTTTGCATTCTGACTTTGCATTTTAGAGCATTTTAAGTATTACCACACTTTTTTGCATACTTCAGTATACTTGTTAAAA(/<CXjy !2=JVajv~ *5GVan|(3?Q]ir(4?KVanx $1@MYdp{ "-7CNZblw%0;GO[gr~  +6AMXbmx   ! * 6 A M X ` k u   ( 3 > I U ^ h s   & 1 < F P [ e q |    # , 8 F O Y d n x   ! , 7 B K X b k w  ,:CNXcoy"0=(/<CXjy !2=JVajv~ *5GVan|(3?Q]ir(4?KVanx $1@MYdp{ "-7CNZblw%0;GO[gr~  +6AMXbmx   ! * 6 A M X ` k u   ( 3 > I U ^ h s   & 1 < F P [ e q |    # , 8 F O Y d n x   ! , 7 B K X b k w  ,:CNXcoy"0=       "      $  ")"+)    "  "       ""     " ' "                   ""(                 "      $  ")"+)    "  "       ""     " ' "                   ""(          \H0a0+&?!4G -&(OU/ O? GL0/P`X&KXU:9QAY + /Q!,)H9QEA >P4H) 4B<T?7.$D"!" 25?:d^4= @9 E@<$\[ cdL*T5.`I PB?B$O 0DL<S=!2 MZCOU[$[PO S4A@aF^S 8**SU&+XM_.+!< ]+HX P.)HRD+JAE3>I8_9SE>W<.a@YUJFG5^_ M"5-$J/X:,9XXRJ+X>  \W#Z# 1a/ A_G<YXa"/ @A.":*_$$ .2 K7MDZCbB;"9 AaL$%?c > j Y0%kTLkS#>xXQ'<c) )hkmw_9pn q; _gLH9k wP V4b ;: K^qE! Z _t]S:<zO? EE&fp,jRQIffcu1H*cS90wd ba~ G[8mI500Bf/sB/#G|^1r0}[+Bos'aV d> V j (mVSLXimGjb@dR^b9<cxJf7?}(L0BFoB?R3 3l   ?) ## #4'j  F ;jQR ,@ ;OG"_`_zE.W3Vfr*H9p@"Z%#8@,8(p2'p 5uDhNX \p'zI/,Ua6}K~p8AI,&Mu/ ".&  :wt("~v}Kp}2DTp 0 }0lExJZR0Uup#orYL<KWu/ IEo&=,d|u, ~{Dxc@ 7" + _ _ s 04/_^ UbS:wlWl/"o5v)d3J>G(UDf&q1 =xKO|-z]([rw=i2CVj&0c`0 SttKZ*xa% gd(3Z"1!~tHt41fUK4s  ho|.?eB eIzPHrD<8-0zpy(yx``Pm l%EUe|5OovH 7<L*1pwo,T!B2r%$L\PvCATTCCCTCTGGGAAAGCCATATACCCTCCACGGGCACAGGGTAAGATTAAACCCTTTAAGAAGGTAGATATTCGGGGACATCTGCCGTCGTAATGAATAAAAAAACTGAGAAGATCTAATTTACCTTTGGGGAAAACTAACCTATAGTATATTAAAGTAGCAACATCATCCGAAAGAATGGGTCTTAACTATTTTTAAATAATAACCTGTGGAAATTGATCCTAGCATCGCAACGTCCTAGGTCTAGAGGTTCCATAAAGGCCTCCCCTTGCCACTTGGCCTACCAAGTATCACACACGGCCCAATTAGATTCCAGCATTCCCTCCTGCCTATTCGTAAGTCTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-05-25 05:59:07 -04:00AEPt3AEPt3APFN APXV2APrN APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTSBufT"DܜCTIDkCTNMqCTOw܌CTTL ”CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DDATA"DDATA"DVDATA"DDATA #*FT DATA #*FT+^DATA #*FTqDATA #*FTDCHTDSamDySN uDye#DyeN€DyeN…DyeNŠDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT  EVNT EVNT"EVNT5FWO_GATCGTyp!!THCFGCEHCFGHCFGHCFG==InScInVtLANE]LAST~~LIMS!!LNTD2LsrPaMCHNMODF ¾MODL3730NAVGNLNE`NOISbOfSc8P1AMX P1RLX UP1WDXP2AMXP2BAXXP2RLXePBASXXPBASXXPCONXXPCONXXPDMFPDMFyPLOCXPLOCXEPSZE`PTYPܔPXLBRGNmcRMXV1RMdN MRMdV1RMdXVRPrN1RPrV1RUND RUND RUND RUND RUNT RUNT 9RUNT 0RUNT 7Rate HRunN""AS/N%ZSCAN3SMED SMLtSMPL SPACMSPACrSPACMSVER4.0SVER SVER,,Satd8^Scal@Scan3TUBEB11Tmpr<UserphARphCHphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTSBufT"DܜCTIDkCTNMqCTOw܌CTTL ”CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DDATA"DDATA"DVDATA"DDATA #*FT DATA #*FT+^DATA #*FTqDATA #*FTDCHTDSamDySN uDye#DyeN€DyeN…DyeNŠDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT  EVNT EVNT"EVNT5FTab(FVoc'FWO_GATCFeat..'GTyp!!THCFGCEHCFGHCFGHCFG==InScInVtLANE]LAST~~LIMS!!LNTD2LsrPaMCHNMODF ¾MODL3730NAVGNLNE`NOISbOfSc8P1AMX P1RLX UP1WDXP2AMXP2BAXXP2RLXePBASXXPBASXXPCONXXPCONXXPDMFPDMFyPLOCXPLOCXEPSZE`PTYPܔPXLBRGNmcRMXV1RMdN MRMdV1RMdXVRPrN1RPrV1RUND RUND RUND RUND RUNT RUNT 9RUNT 0RUNT 7Rate HRunN""AS/N%ZSCAN3SMED SMLtSMPL SPACMSPACrSPACMSVER4.0SVER SVER,,Satd8^Scal@Scan3TUBEB11Tmpr<UserphARphCHphDYbigphQLcphTRphTRFB}}Cg/D&Wr`s;bqz^]/r$^.>6pe:*>^Jp@48Pl: &Pwi~69b[dWUlh{.4eY\`;w>xPqL6`nCRNX}a/JBcw*jEth@t#Rt+7+:}JcLL-GIA2oz:4JA^V\h}c%kU?-}U&0;S3ZfYUgB\(<({]kId`Po<_+MWQ}VX*oE}`Z&oRLV;);-ags5)9mW'>YP~jhM^,%bkb |{c|5<&n5N`!1;U*W]S#|Y;;y+Y3y'E3ro{`qaL`hlZZdBc'@wMXC5K;@WJG7jVlG0$_9'mSTve!@;Ps>4]F%65r#W3}hxv_1=|8Ifz2wHiD(g=0ia}yI?t_tG|T`S'_[PTcM#yKAu22`ArIxT6e&?bFW3|>oW~!Te4;,: TZc:=?t)bmZ\Fq+l5AF52p$.jc_T3 #pJE,#VuU,YTl_u4TYe0tpJh&j43ofR5*6Y?Qs?]t~1{[q?Eb,T2|5iZFod>7:I12mxXYnuPQh0{!X<(&}J39OQJM~6Hrd+v/gg61CM#Y\VL&p?)_QQe|vH[p!hjqf[MH-YtDSYcJ|_nO*rS4,vcM&3#GQbS9YH|O[nYnuPQh^5Q1V?yRl<`QV-@[3>>BZuGZ-p@vulc*{<=pjHOo&eG1Vcy[*oCf`y6n?2x`agv`kt3G/3t\#9/hV6T]==6X12O[n/bsDU5hf61FA>&oPWTRI1]f)yA@r9s_|g+*F}t.("P~fI2P&N+E/Gz^n4.6t}VX*os7}Z&[Eb4;,}s@dUa0u:W^$B!X`7.ykI0o6Gzv&1fu4WyYZFU5FS7&3-OT5ro{`N%7`h?&03ghW~^(';_/C]^HLl}5.X}=$~I!|sp#at.D>["[87PFdPxKLh{*l5L|&vBg-' 'DvddD\4A$a{|ds:MkOVc2(_CL,v.h}{ZE!]Rc{_@w,iP;YCWeq }X'>n~~2q>-kb+N4+07> YlGcRb>@iG-@yy3@=?]p&qN<;u4#k:X=<;s_|g+rapPj:,xNM,[*?BRCZcV]j?:{VxzTA=a:hVUDBW39Zm@T(ApKAbZ]"Po1Cd}F<_`VWYH|O[nY\Kwoh^*p%!++(aQQ*G]mysG(B]mzQLAEPt3AEPt3APFN APXV2APrN APrV1APrX}}ARTNASPFASPtASPtAUDTbb7B1PtB1PtBCTSBufT"DܜCTIDkCTNMqCTOw܌CTTL ”CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DDATA"DDATA"DVDATA"DDATA #*FT DATA #*FT+^DATA #*FTqDATA #*FTDCHTDSamDySN uDye#DyeN€DyeN…DyeNŠDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT  EVNT EVNT"EVNT5FTab(FVoc'FWO_GATCFeat..'GTyp!!THCFGCEHCFGHCFGHCFG==InScInVtLANE]LAST~~LIMS!!LNTD2LsrPaMCHNMODF ¾MODL3730NAVGNLNE`NOISbOfSc8P1AMX P1RLX UP1WDXP2AMXP2BAXXP2RLXePBASXXPBASXXPCONXXPCONXXPDMFPDMFyPLOCXPLOCXEPSZE`PTYPܔPXLBRGNmcRMXV1RMdN MRMdV1RMdXVRPrN1RPrV1RUND RUND RUND RUND RUNT RUNT 9RUNT 0RUNT 7Rate HRunN""AS/N%ZSCAN3SMED SMLtSMPL SPACMSPACrSPACMSVER4.0SVER SVER,,Satd8^Scal@Scan3TUBEB11Tmpr<UserphARphCHphDYbigphQLcphTRphTR