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a @ ^ H H E [ G 9 9 8 F @ G < 5 > E N B 1 J = Y E Z W J > H _ E L > L T @ A @ H > ? G - < = = . B % , N = . 3 0 B B @ F O H ; @ D < = 6 C = 1 P B E @ ; C J U D Q B @ _ ) C A J D K B D L M 6 @ M # D O A < 2 L B C H Y @ : I > X B C @ 5 : 2 9 : @ = 4 + 7 . C = E + D 5 B C : H 8 ? O 8 @ A A A @ J L ? L M ; E ? I C : C T H < : F M R @ F = T R V E C T B J > F R S R @ B > F H 5 O 1 = G J I O D G > G 5 P B @ 0 < > G : ; = 8 ? I N K 9 < G , + B B ; 9 C ; ; J G L V A 2 F H ; H H T 5 1 ; C ( 5 = S G Q @ @ = 7 3 N : 2 : S H B G C = 8 Z T J L D K 3 H J @ I 3 : V V L F 3 7 4 L A I E O J K E G S L B P G P I E R < X @ L K I D R A B G H 9 = G . ; K 6 ? / 9 5 > = B Z 9 1 5 , @ K : 6 E @ : 7 S 9 O N \ [ L J T C L R I a [ N r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i Z Y Y G B A U B Q O 8 ? < C 5 3 D 9 U U C 6 K H D N M @ Q C C Z R U [ I ; 8 R K F I \ R 3 D < = J Q E = @ ? - > N O > : 4 3 G 0 < A / ? > 6 I B : < @ O Q ; 7 2 ? > L K = < 6 ; > A < H ( # F 2 F = K 5 5 ? @ D 6 6 0 0 D B ? 6 4 3 < 1 B 4 ; A 6 < ' + F 8 B ; 6 5 ? ; ) ' 5 : = 8 A M E ? : 8 . 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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: db59c7dab4a64635a5174b0c4a530e4fSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////
////////////////////////////////////////////////////////////////////////////
//////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB
AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA
AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA
AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA
AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-05-25_08-59_0787 3130Seq 12516 12516_Costion_K1314_matK_R jmadera 96-Well
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/ A L (@]AA<-AKB.bcpKB_3730_POP7_BDTv3.mobtermNNNNNNNNNN
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" . : NNNNNBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-05-25 10:47:02 -04:00AEPt 3 AEPt 3 APFN 0 APXV 2 APrN o APrV 1 APrX } } x ARTN v ASPF ASPt ASPt B1Pt B1Pt BCTS
l BufT D CTID CTNM CTOw 4 CTTL < CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA ( DATA DATA DATA # G 9 DATA
# G 3= DATA # G zA DATA # G E DCHT DSam DySN Dye# DyeN ( DyeN - DyeN 2 DyeN 7 DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT Dz EVNT Ǿ EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 9 LAST ~ ~ LIMS ! ! E LNTD 2 LsrP a MCHN MODF f MODL 3730 NAVG NLNE ` NOIS Q P1AM
P1RL
P1WD
P2AM
P2BA P2RL
PBAS PBAS PCON PCON PDMF PDMF h PLOC
PLOC
PSZE ` PTYP < PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ; RUNT
. RUNT % RUNT
+* Rate RunN " " S/N% I SCAN 3 SMED
SMLt SMPL SPAC M SPAC a SPAC M SVER 4.0 SVER
a SVER , , dž Scal @ Scan 3 TUBE D7 Tmpr < User phAR phCH phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN 0 APXV 2 APrN o APrV 1 APrX } } x ARTN v ASPF ASPt ASPt B1Pt B1Pt BCTS
l BufT D CTID CTNM CTOw 4 CTTL < CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA ( DATA DATA DATA # G 9 DATA
# G 3= DATA # G zA DATA # G E DCHT DSam DySN Dye# DyeN ( DyeN - DyeN 2 DyeN 7 DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT Dz EVNT Ǿ EVNT EVNT FTab FVoc FWO_ GATC Feat . . GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 9 LAST ~ ~ LIMS ! ! E LNTD 2 LsrP a MCHN MODF f MODL 3730 NAVG NLNE ` NOIS Q P1AM
P1RL
P1WD
P2AM
P2BA P2RL
PBAS PBAS PCON PCON PDMF PDMF h PLOC
PLOC
PSZE ` PTYP < PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ; RUNT
. RUNT % RUNT
+* Rate RunN " " S/N% I SCAN 3 SMED
SMLt SMPL SPAC M SPAC a SPAC M SVER 4.0 SVER
a SVER , , dž Scal @ Scan 3 TUBE D7 Tmpr < User phAR phCH phDY big phQL c phTR phTR f\mci2aqN8>o Ueh,9n5g~Ri6(*24;x$}FNVUCs-113xT]o7U"y6^i_y>]y&m"o`^ kS\KQpYp&!gL-GI&7.{Mco6m~2HBIUbe1Cd}FxQZEZ,}4HUXYnuPQhoNePv7b{Wj2oFtXyy3@=?]p&)C^DZ=CHTA,h{|dsYuTF}wK[*oCfo;LY&p5S_3I|o6HvFHZ( g>}WqD@~TlW+b@cT]`AqhDYA`N($OWtI2c+-@[3>>B%P^gWT^Fu*t&:_Gr9#(+gbk1^/.S3xCmqy=Y`gsB0PPOw[h@tC]Q#V>Ax`X~lTmt.qK|-o3o*oUnY!9s!&&v_QjXaM~3>>//x3L#Id4|Ca$"T'MT,Tc%ToN668d#w>6?xHf.QR9JK[YlyjSu30&reA 03OZgi4$mXArV/^Z+:hN{+eqL}4|Md'ac_`k(U.*C]J=E]1|eKW3CNIG2gbXW,VE+0&'7%4X+*fl979v$sp3@=?]p&q!R;{?kJ:h+p?H(+Q;T`h3/[ze/G,y"o-,(B~b) e:=H)RiA9GtXWJoe`rlx%b@cTY_eM6^sO \}!&R2{bP+z,2L]iw)9Elwe*vddD\4A,h{|dsgbk1^/.S3xCmqL}4|Md^_w&;H26.?bFW?E)X~6"den?,}&8)TK7\4v3s'kY6C}B-<`}l#[GVsI.J:*yEwiOfFD\4**+^cfoQek>HO7T3|2f[F6.9bEd9gP`koGhnWe^kQtAqjM>P\;Er|Dnm$7uM2^7f!]47.<2*VSU(]y"/:Boz?5wz^`Z#XTE-yh;}S$OKOfx&iJmW+E+n[yrA]#7w"OMbI.*R@_ajF=j&3d}7&I]N@c'v+gK >xPo\W'KFkaqQltX>V