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K r V H N P Z S ^ E ^ M U Z L ` V B R M E S ] [ > g M ^ d Y c e Z ] G ] i h N F 8 > F \ g D K P H S Q W K < X _ B Q P v c \ a Z K O U @ U T U / O F b [ L e Y [ T ` Y ^ F Z V Q K E G l A H J ` x L c l l n c Y I L a b Z N N K W a V 1 i e U ^ Q ` \ M A R ] P Q R b k X M L o W ^ [ \ o N H P D V ? Z V T G S O L D F V d ^ b K S O M B X L V m f V K C K e U R : @ j Z ^ [ S R m @ S Z M j c ] V b T b f ] d _ S c Y E D l b P b l R > ` c Y S ` L T O e q q h ` I V R J H R ] ] T T P c k _ U a d p X \ L P e ] d X J c N T ] \ u c k V C Z G G W D G C A \ J : M p U H G M @ Y B V P T K E T A _ c Y e H P O G Q O W R L R a X T E S Y T g U c T 9 \ G G R S N Z H S b b P E A Y d C ] 6 . ] f [ ? J _ b B U Z 5 L Y B N V \ ^ O 9 R L Q a D c l ` O I D [ g T J J T [ b N _ H V F E O R N \ @ V c f V B O K N H J J [ X P i ] E F \ Q S e ^ O N I W Z _ e 8 L X I J Y E C K ? C R g A ] L G \ A K U 5 B E = : i K I N T K X j K E Y A H F C T o _ L B W v ] e q l a S S J C f ] a ] n x H Y Q N ] D i W W > a U Q d Q H D K M ) N S B X I I C V c E Q 8 X g H \ K Q \ \ E N V H h L d C A U J i ] B ^ X b E T ` U c ` ^ T h M X P b u ` l P d K H 7 I E I ` c a k e ^ l Q P \ P C Z W S X Q @ n _ W K d R M Q [ P N g O Y b d Z D b P ^ I g R 7 g N g d L L ^ b T J J R Y E L W > W R S F ^ G _ T J _ F @ N E C N H ^ ` G J N L A Z N Q P 3 M Z I B P < [ [ V K ( K W ` W X S > R W ] R \ ^ _ P : F Y D [ F T B Y R b H l Q Q E S R R R E S q R 9 ] g ` @ E I Y c N ^ Q N G I < O G U Z V ; P \ B P N j J \ P X K I 9 @ U J H M d X g = Z ; B T N E H ] M ^ B P A N / I F D L ; a U J D C s | s X R P Q _ g G : h Z W U U R Y L \ ^ U S < M f q P S E b R C > B Y K O N [ V ; H U T V [ O Z W T M : I < L U T L 9 Q 5 V O Q Z M A : b K < H ^ W R h @ R 0 Y ? R _ W ` H 9 D T A J O A 6 V e B Q H 1 X ; 4 < , 5 j I 4 U G U O = W _ t P a T D D C T _ ^ U V Z F = I c 4 ^ W f K M ] > D O : L S V K e I N B C 5 Q Y X ` = D > C D h 0 [ C M = W = N _ A S ] G G E p ` T D Q Q X U R ? A I X c D E R k F O ? E G [ I @ a T M B P V K 7 ? > B 1 D H P G M D ^ K B I \ F g @ X ^ Z a V G K J V S K W G N V X c P R S V e \ U N T = E ^ H = ^ U V ^ O \ 7 > _ I C W r 7 U B g < A A Y W G B D 8 M < S g Y A N G : Z E G U ] b H O j N N G _ l j > J d F e C P X X ` M O Z J C N X a _ U e X @ P E p U k e N a i A Q T [ a 8 ] H ] J Q T ? R m Y K G P 8 P J M U S L T W _ M L U 9 > R Q U ? ` M E Z L ^ R T T l T M M Y O c p X K a k W [ _ T 4 Q G Z U I j m Y ` [ A O Z n ; N N R _ B ] Q U Z U 2 O W \ M J ^ R 5 ^ K U A M K y H j 9 p Y J R I S D G P K Q Q = C X P ? S V F N X _ S D D g O M Q 6 W b _ L R J A F X ] L R J I N I c ` E @ B K > [ R B T I G [ > K Z P D H ` I X < c P E Z A R N ` A > > > \ G N B Q W R F > C R @ C @ S I N K J A ; E R O K U g 8 0 c Y D Q G 9 G M H : a N C F S T J F h S M : Q Y S [ ^ W R T M U E b W W X ? G Q S Q B \ K F ] J T G A ` . < Z Z Q . a X J J Y I K U [ N : R W a I K L Q @ > U V J ` G H [ K Q b \ \ = ? C L G W X F < A > < > C Z I e ^ M 3 D Q D I D Z K [ P f [ ` W W C \ W @ T K O Y o S R X R M L b E G ^ S d J U f 6 E s W P S T J z Y ` ^ : \ Y Q n K T I h D S D P O K ? B N I M [ t ] K J i V ( K B p K T H S J R ] L S L R _ ' P K f P [ M L N Y V R L P Y @ ] L H Q P N L Q U D ] L F T X L X Q Z X Y W Z Q ^ j S j M Z W Q T S C P n ; M 7 J A 7 5 P L ; d 7 A W ^ X C R J F R S 5 9 9 X C J W 1 ` U F M M 1 S = M Q P f K F S c K G S L 9 G R K \ D I K A E F 4 F J P L T A Y U P Y W ? c M A G F \ I = 4 C > U = c ? P S o Q T I C ? F C X J T h M J T ; N S = @ J Z U V : < N K I ? 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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: f7c9956e7efa4c868fa1f0725fe31bfeSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-05-25_08-59_0787 3130Seq 12516 12516_Costion_K1314_matK_R jmadera 96-Well
K1314_D1_RKB_3730_POP7_BDTv3.mobSeqPop7z 5 H T U K 8 # * , & 1 N i { u ` H 1 ( V \ ' / W q v k [ X k H ; Q b v .>* z P A K e q H 0 8 b d 8 ( . > J L D : 1 , % ; i h : ) ? K H : ( ) E g x b U Y n :F7 T RlX%*;b^5Z9~o f
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" . : NNNNNBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-05-25 10:46:17 -04:00AEPt 3 AEPt 3 APFN 0 APXV 2 APrN o APrV 1 APrX } } x ARTN v ASPF ASPt ASPt B1Pt B1Pt BCTS
l BufT D CTID CTNM CTOw 4 CTTL < CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA ( DATA DATA DATA # G 9 DATA
# G 3= DATA # G zA DATA # G E DCHT DSam DySN Dye# DyeN ( DyeN - DyeN 2 DyeN 7 DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT Dz EVNT Ǿ EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ LIMS ! ! E LNTD 2 LsrP a MCHN MODF f MODL 3730 NAVG NLNE ` NOIS Q P1AM
P1RL
P1WD
P2AM
P2BA P2RL
PBAS PBAS PCON PCON PDMF PDMF h PLOC
PLOC
PSZE ` PTYP < PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ; RUNT
. RUNT % RUNT
+* Rate RunN " " S/N% I SCAN 3 SMED
SMLt SMPL SPAC M SPAC a SPAC M SVER 4.0 SVER
a SVER , , dž Scal @ Scan 3 TUBE D1 Tmpr < User phAR phCH phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN 0 APXV 2 APrN o APrV 1 APrX } } x ARTN v ASPF ASPt ASPt B1Pt B1Pt BCTS
l BufT D CTID CTNM CTOw 4 CTTL < CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA ( DATA DATA DATA # G 9 DATA
# G 3= DATA # G zA DATA # G E DCHT DSam DySN Dye# DyeN ( DyeN - DyeN 2 DyeN 7 DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT Dz EVNT Ǿ EVNT EVNT FTab FVoc FWO_ GATC Feat . . GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ LIMS ! ! E LNTD 2 LsrP a MCHN MODF f MODL 3730 NAVG NLNE ` NOIS Q P1AM
P1RL
P1WD
P2AM
P2BA P2RL
PBAS PBAS PCON PCON PDMF PDMF h PLOC
PLOC
PSZE ` PTYP < PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ; RUNT
. RUNT % RUNT
+* Rate RunN " " S/N% I SCAN 3 SMED
SMLt SMPL SPAC M SPAC a SPAC M SVER 4.0 SVER
a SVER , , dž Scal @ Scan 3 TUBE D1 Tmpr < User phAR phCH phDY big phQL c phTR phTR ,*^(`P. O~JcW`@0'q qoVU-9k *c@{UaG6EG>dcnxgJB7`DK,*Tijh.iOW/D-]$/pTFV2TVyLyD,kSagv9'i?oX$^io4)X^UM]|ny!.qK|C!^g:G1|*dc0U+O}L6iyNmwP@rB ]:l=&3#Veqy{g_hD6z\[N.6| Vkyc p7xMO9j;q6TIUwch.sQ@_=!)}W}oQln:~]a:WkR,uKsH"Kg5!O$AUI/vO3G`8hC0rOHJJr9b6mtTUk1T:29"5-z]T1S6RA0W7yLlo/&U{uclQ[vL}W$P1ObYn#pwW(YzXr$cn?:;wWG{uHYs+/e_'UvBVvwp#_T99Wq3F:{SocJy>@ YlGcRb[xy8J9tG>udrV=y`6@TmkcDynWa9?QLwUF3TP.&"D|;f[5kr+E{@2KmU1F*+e<7McXDo#UW!\`C}B-mq5FaT9Vbr$rv/gg61CNdW)j-[YD8J)T(k"T'oA_AN;)oX6YMI)0g'^+oL.SVX*os7}Z&['Ko;X^`>EOCy KQI5m6``^,Ffn@axsA MG }OPmE$ww*{we* _ZZ^"4/GKTrQ3oS"Y|8by`AVih{3r< wvr]LaiY2]NDzU4obir8rP p'Ic|4r;4r.=:!rY,4wb9mz~oq_6k99/qNE^O{3fJ:hA?I| )TuTF}T\Jpx _,y".9W2Bj:w`kPhL`A(^:go8m+adJs _ZZ^USn&vrOU[.(3`C/fshkr+E`_W>WM@A<}1X*#PE|G#]9Ug#jmIn(?$OA2nrD5pYlGcRb[q5FaT9Vbr$rv/qNE^O{|cHT?Ou4|8e7spFp[O6h"4#O|!f|bEdG;_{8@hh/WZ#3|)l>,S]ZD>tSEH$*YYo%+0D5p>?e t}1%jF}up"GrM7VpCJT|Fu*qcjhAhE;qT%;5]U' S!oS\[Z*YaRsgGw>2Mcr.Lk@_aP4rRj"SD=y!.qK|N$t.DkFhwD~EG7&o.AiG3mwYB{,9)-]HqQIxw}H{-*