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" %        $ "          /%$%! %                             %9:;Z~R l X{NlhEE<$jYdiQ7z~wus\a`_OL>IE><7427(CNPHi  yyXI*+4&       " !/*Qam{qhON@5'+   #"#,%))-)&*  10-0/'=SarW<?%(*>RfszU< $                 0(/),              $# "   %                                                  )               ,,,IIT^^{{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR(    WZZQQQrrr   %%$///:99DDDNNNWWWa``jjjsss{{{ !!!""""###$$$%%%%&&&''''((())))****+++,,,,----..../////000011112222233334444455555666667777788888999999999::::::::::;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: bec51245ccfd4cee989dd367075f157aSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-05-25_08-59_07873130Seq1251612516_Costion_K1314_matK_Rjmadera96-Well K1314_D10_RKB_3730_POP7_BDTv3.mobSeqPop7z7\&VfB}8w  ; T$a/1?13 j !6!S!M!=!2!0!(! 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P@˒APHAB&A KB.bcpKB_3730_POP7_BDTv3.mobtermCGCCCCCGTGCCTCTGAGACGAGTGATTGCTGCGTATAATAAAATAGTATCTGCTTCGCCCATCGGTCGTTTATAACAGAATCTGAATGAATCCGTCCAGGTCGATTCTAATGGAATGCCCTAATACATTAAAAAATTTCTCTTTAGTCATGTTCCATTAATGGAACTATTGGAATTTTTGTATCCCATATCTTTCTAACGCCCCCGTGCCTCTGAGACGAGTGATTGCTGCGTATAATAAAATAGTATCTGCTTCGCCCATCGGTCGTTTATAACAGAATCTGAATGAATCCGTCCAGGTCGATTCTAATGGAATGCCCTAATACATTAAAAAATTTCTCTTTAGTCATGTTCCATTAATGGAACTATTGGAATTTTTGTATCCCATATCTTTCTAA=DWcv$8GU[jx $/6@FR\u-@N\kt&/=ELSamz!4IS^ir",:ELZgr{ '3BJU`jw &1:CMYeny   ( 1 <=DWcv$8GU[jx $/6@FR\u-@N\kt&/=ELSamz!4IS^ir",:ELZgr{ '3BJU`jw &1:CMYeny   ( 1 < #     ; !                     "   "          #     ; !                     "   "         MC'B2HM:<,%FG)7Z<S&UT'+;%I,+*!& J ;  ]QaPFK441),P $,U(,7 1S "649T'B(*,H[ .&`TR%L IPD*`HRVHD0XDTD57P3M7% b3]P9=8:+@T.2C:K&_5 (-UB]6J 9  7 H! <+HU7=p&d@~UH? *  %Dt=*bcd^dk[wJzP zZ:T2D!t4HOWAJ\4mk #u.+]#>OM>GNv,# b9 CKdtTT%i8av8f "T{GWbno IRTk13F=r0z 1+DU)$ Pu tIppLpH)xP 'z& + L\]vdBe@ZBwYxq<\F{&^>-m_k e?~4>t<)f8x8.hF' :;j1g[$PInD"a!.kU-uC(- g/h8a@,7hPH:-zrL&[xzyrT^F H$X qV:f^R0<3N\fO~u*.Ao <4v,?V c]>'>#{GTAAAAAAAAAAAAATGACAATTGTTAGTTCCGTATATTATTGGAGAAGATCAAAGATTAACATCCCTCAGATATTAGATTAACTGGCACTAAAACTACAACTCCCATCCTATAGGGCGTACCTCGATGATCTTTTGACTCTCCGTTCTTATCCTTCAACCCATGTAATCGTCCCCAGGATGCCTGATCTAAAAATAGTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-05-25 10:47:18 -04:00AEPt3AEPt3APFN 1APXV2APrN oAPrV1APrX}}xARTNASPFASPtASPtB1PtB1PtBCTSBufT DCTIDCTNMCTOw4CTTL <CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA ^DATA (DATA DATA DATA #F<:DATA #F<2vDATA #F<xDATA #F<DCHTDSamDySN Dye#DyeN(DyeN-DyeN2DyeN7DyeWDyeW8DyeWSDyeWgEPVt!4EVNT DzEVNT ǾEVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEJLAST~~*LIMS!!ELNTD2LsrPaMCHNMODF fMODL3730NAVGNLNE`NOIS2P1AM +P1RL P1WDP2AMP2BAcP2RLGPBAS,PBASePCON PCON PDMFPDMFIPLOCPLOCPSZE`PTYP<PXLBRGNm RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT ;RUNT  .RUNT  %RUNT  +*Rate RunN""S/N%*SCAN3SMED SMLtSMPL SPACMSPACBSPACMSVER4.0SVER SVER,,džScal@Scan3TUBED10Tmpr<UserphARphCH`phDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN 1APXV2APrN oAPrV1APrX}}xARTNASPFASPtASPtB1PtB1PtBCTSBufT DCTIDCTNMCTOw4CTTL <CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA ^DATA (DATA DATA DATA #F<:DATA #F<2vDATA #F<xDATA #F<DCHTDSamDySN Dye#DyeN(DyeN-DyeN2DyeN7DyeWDyeW8DyeWSDyeWgEPVt!4EVNT DzEVNT ǾEVNTEVNTFTab$ FVoc#FWO_GATCFeat..#GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEJLAST~~*LIMS!!ELNTD2LsrPaMCHNMODF fMODL3730NAVGNLNE`NOIS2P1AM +P1RL P1WDP2AMP2BAcP2RLGPBAS,PBASePCON PCON PDMFPDMFIPLOCPLOCPSZE`PTYP<PXLBRGNm RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT ;RUNT  .RUNT  %RUNT  +*Rate RunN""S/N%*SCAN3SMED SMLtSMPL SPACMSPACBSPACMSVER4.0SVER SVER,,džScal@Scan3TUBED10Tmpr<UserphARphCH`phDYbigphQLcphTRphTRc:]FN}T3pb}045@u:r*nOroz*N^YEu'+=3yHSBrRLohL)N6PagEU0T6;e=Y"sWi|Cal(i#5h1g9%TFIO?z3e]/\(fp%Jp,@jJo{l(l>6,8efPmE$w^6Rwe7sE^Z{F9LwU$<;172+C~6bfm{l|?"wdp$^HApEnFiWb)jN-:d=iU~Hfzq?;o4O0uStM44kJ#>QBQ8p[UR6? sI(J8dR9F![pE)}%z5dJ6vvdeW\j//u4aO/CsINi@C)Re/K)Z6AI8rBAr] {[q?Eb,f=E]rRS$~=}F)h>S]p?_sDkrD5pYlGcRb 2)FB[yEZ>f-6>C#!^Cu_OlYc?pTtfQgvv6=fewsB+(t#ji_~5|vroW~!^F7N? >S]T'_C:[rn{.QHT"Hf.Q F3eG"k@j/.3]H6X+9mK\TI_aEdO{7+VfU~oM6CqwToN668d#t[XS<< x3]aC^D~i#aYP+p?H(+:MkO@JC,E`fW7.5k|_%Z]R'OEte1}1"/"g7bxd$XF${WhcS]U$3g;Z6ObePWz"$b[q5FaT%ycX}nz:z5>vQSdW79ZYdj8WoQK5F1_;T,V2%4oNYkk?[v?3XdWRd+xHN+a,|&bX*F8Ba+b8EYWC.%oKO> `VsIefaqQl&A6PG>}Oujd+[Y.m/wBN $`?"*J[x5FaGwpBT9\qwH=qd)B*vH%lF1?HOeCvpN^3jNRjnMgx*&`R@){wJUo6X?/WZ#&a:OcP$SXtmx+9Hgn<_swW^$buJrUlM;_}$A>e6bZ( iu2Zv&1`Z=WcDArh]|Ko5qS+SP7T]TcSI*$6ey(B~bea6:!.tk}Y=52rR~o[k7MLr92U*a!