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[ [ R R R R s s s '''111<<<FGGQPPZZZdccmmlvvu}}} < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: d44686a538c044059bd280c1205a56e4Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-05-24_08-39_0774 3130Seq 12506 12506_Costion_K1316_matK_R jmadera 96-Well
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2% xd s ` H -T 8): /i rY? ^ $@ATTATAAATCACTCAGAAGGGAAGATAGGCGATATTATTACGAGGGATAAATGCACATAGACGGACCCAGCGAACGATGATAACAGTAGCCGAGGTAACCGGGCGGAGCCGCACTATTGAAATAAGGTCCCATGAGATACCGGAAATCGCAAGAGACGGAGGTACCATCAGAAGTATTAGTTGGAGGAGAGGATCCCTCCTCATGAGAGGGAGAAGGAAGAGGGAGGGGAGCTCGAGCCAAAGGCTGCCCGGCGGAGGAAAGGAGTGTTGCACTCGTGGGAGAGCTTACCCTGAGATGAAAGGAAGGGAAGGGGAAATGGATATAGAGACTCCTTGCCCGCGCGGGCAGCCGGGGGTACGATAGATTCCCTTAAATTCGAAGGGATAACTTCAAATAATCAACCGGAGAAGACTTTACCATTGATACGCTAAATAAACTTCGTTATTTATCACATTGAATGTGTAAATATTCGTGCACCAACAGAAGGTCGGGTGGGTATTCATATCGGACACGGACACCTCTTGACATACTATAAAACTTCATAGGTATATACTCGCTCGAGGGACGCTACAGGATATGGTTAGCATTTATGAGGATAGTTGGCGCATAGGGACAAGCTTGTATACTCATACTCATATATAAGAGTAATATATGAGGAGGAGGAGTCTCAGATAACTCCAAGAGTTGGAGGAGATATATGCCCTCACATAATATTATGATAATACTACTACACGCGTCGAGGGAGTCTCATTATTTGTGCGGAGAAGACGTACTCCCTATATTCCCGAGAGGAGAGAGAGCAACGAATAAACTTTAGATAGCAAATGTCCTGTGGGAGATATATCCCACACAACTACATCTGTGAGGGGAGGTGCTTTCCTCTCCCCCTATGTAGTGGGTGGTGAGTAGAGABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-05-24 10:10:22 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA ,1 Xb DATA
,1 Xb < DATA ,1 Xb _ DATA ,1 Xb DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE G LAST ~ ~ ! LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS F+ P1AM " j P1RL " b P1WD " q* P2AM " n P2BA P2RL " xL PBAS I PBAS F^ PCON _U PCON [ PDMF { PDMF FB PLOC " T PLOC " M PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ' RUNT
RUNT
RUNT
1 Rate RunN " " S/N% F# SCAN 3 SMED
SMLt SMPL p SPAC AV+ SPAC F; SPAC AV+ SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE E9 Tmpr < User phAR phCH FY phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA ,1 Xb DATA
,1 Xb < DATA ,1 Xb _ DATA ,1 Xb DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat / / GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE G LAST ~ ~ ! LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS F+ P1AM " j P1RL " b P1WD " q* P2AM " n P2BA P2RL " xL PBAS I PBAS F^ PCON _U PCON [ PDMF { PDMF FB PLOC " T PLOC " M PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ' RUNT
RUNT
RUNT
1 Rate RunN " " S/N% F# SCAN 3 SMED
SMLt SMPL p SPAC AV+ SPAC F; SPAC AV+ SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE E9 Tmpr < User phAR phCH FY phDY big phQL c phTR phTR mkYbCK)Ul_pD8Jq,./b _.uKxh6O1w^{l{iED>{?oH^}nZ##A+]WJyKPy]Y !T_*|QK:jin'D5?C7bJeoiQ]%[p3}$r&kUV'4MYkftTI|Z{Z[{%;5]U'Q>?";!8L[2Rr`gPV]O_,~])RpikE:b1AmX-RMLr92U*a!(G2MZLNN1O3W2]Q{Y^@>=,8,?qm#v6F[(vW3TA=]I:<{i(os1tog,s48h(PW[9?Db2@&2$U^X$^(`])>mJGVGkB*Sig]r;a4xG57'!>&7.{Mo XSr$LVYyo6-I1]f)yA@r9#(2g|ea}w*ohj4Mj2Y6YZ_SGmm{k=!.t;Z\3|7hhSc%bVm? : wT_{.BWr4O%\4G(^oL%i-{mwYo{A(PmE$ww*{we* _ZZ^k_o5qS+SP7T]^q;,`h?&X`hB`}_AvG9 HtWo0<8WFoe~8WYH!KPk;+s4U5hf6]X?K90lVSUFGsbrudrV3]wnF){xD\4AFU?-4$&#S%TwLhl69e >.8#9!yv`R/ Tgfyml{W7;rRU)>`V2]<|'kp?_sDk`457^^s?92>@iG-@,P> u)v/|vu~CM#Y\Vd*U5aO/CsINi@C,{s2fo;LY&S2Bjr<|Ate1}1"/"@-bWF4oFo;X.: wT_|owoqiY.] z,&0@1>X`]yVlX/-f>P5CRV1]cK=i~f&X@EFF~lbq&S)BcS[7}?ef5U_9}j~:HzZbti2]2rRU)>`V|I+!K|-o_}4]'b5 ]{7HFwMzR9vAMV^@c O*Pv#$6wC(vJKq&1hE;qT%;5]U' S!oSRIoM/V/5`agvb_@U])%,1+7XM&b$SXtmx+9Hgn<_swW^$beP`C:F__diM(]y"/:Boz?&qNR0Xwt2)lOY^k'eaK|`Bt#2ze/z,(,y]18`F7IOo_3@/C 4O$ AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt AUDT d d B1Pt B1Pt BCTS BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA ,1 Xb DATA
,1 Xb < DATA ,1 Xb _ DATA ,1 Xb DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat / / GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE G LAST ~ ~ ! LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS F+ P1AM " j P1RL " b P1WD " q* P2AM " n P2BA P2RL " xL PBAS I PBAS F^ PCON _U PCON [ PDMF { PDMF FB PLOC " T PLOC " M PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ' RUNT
RUNT
RUNT
1 Rate RunN " " S/N% F# SCAN 3 SMED
SMLt SMPL p SPAC AV+ SPAC F; SPAC AV+ SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE E9 Tmpr < User phAR phCH FY phDY big phQL c phTR phTR