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[ [ [ R R R t t t #((233===HHHRRQ[[[eddnnmwvv~~~ < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 8568f0a22b1245709afd488162569cf5Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////
////////////////////////////////////////////////////////////////////////////
//////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB
AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA
AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA
AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA
AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-05-23_19-34_0766 3130Seq 12504 12504_Costion_K1315_matK_R jmadera 96-Well K1315_C12_RKB_3730_POP7_BDTv3.mobSeqPop7z + * * ) * + - / 2 3 4 4 2 1 0 / 0 0 1 1 0 / . . . 0 3 7 < A E I L N O N O P S Y a k v "Ek(Ov"R*Mo
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rAATTGTTACACACAGGAGGGGAGAACAATCTATATTATTAAGAGGGATACCTATATATACTCGGGCGGAGCCCACTATTACAACAGTATCAGATTGAGCCGGACAGACCCTCCCTCTGGAAAGACGGTCCTAGGCGACACTTTAACTCTCGAGAGACTGAGATACCAAGAGAAGTATGAGTAGGACGTCTCGATCGTACCTCATTAGAGCCCCATCGAAGAGGGAAGTACCCTCTCGGGGGAGGGGGCGCTTCCGAGGAATGCAGTGGTGCTCTCGAGTGAGAGAGTAGAGTGAGTCGGAAGTAAGAGCATATTAGAAGAATATATAGACTCCTTGCAGGAGACAACTCGTATTGTTATCACCGCTACAGGTAGACACCGAATTATACCTACAAATAAACAACGGGAAACACCTTTCCAGTTAAAACGGGATTTAAACTCCGTTACATCTCACATTGTACGTGGGAATATTCGTACACCATCAGAGGATCGGGTGGGTATTCAGAATGGACACCTCCACGGTAAGCTATAAAAACACATAGGGTATATACGCGCACGCGGGATGCTACAGGATATAGATAGCATCTGTGCGGATAGTTGGCGCAGAGTTTTGTGGATAGATACTCATACTCACATATGTTATTAATATATGAGGACGAGGGGTCTCCGTTAACTCGGGGAGAGTGAGGGGCTCTATAACTCCGTAGAGTTATGACACTACTTCTAACGCTACACGCATACGAGGGAGTCTCATTATCTGTGCTGAGAAGCGTATCAAATTTTCTATCGAGGAGAGGGAGTCATACCTCATATGGTAGTGTAGAGTATAGATATACTCTTATACAACCATTTGTTGAGGGAGGTCTTCCTCTTCCCCTABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-05-23 21:06:02 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS b BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA * U DATA
* U : DATA * U DATA * U ~ DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE \ LAST ~ ~ } LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS <| P1AM n ^ P1RL n X P1WD n e P2AM n s P2BA n n zk P2RL n l PBAS n n @ PBAS n n < PCON n n T PCON n n QC PDMF | PDMF < PLOC n Jg PLOC n C PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
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RUNT " RUNT RUNT 7 RUNT Rate RunN " " S/N% 7y!R}*D]d0oA{Qk@ /kXSE@a!)DS *n&-HOnO@5}"a'3.5s\T7}{W_z>FfM~V0Nt'u'g2R&}ulfwGw{R7R>(hZ$~I!|spN$t.D~F45$\j|KA[Lz2fJq3IxdO#rTi,B)~HFF~X|#&S)BcS[7}?ef5U_9}j~:HzZbti2]2rRU)>`V|I+!K|-o_}4]'b5 ]{7HFwMzR9vAMV^@c O*Pv#$6wC(vJKq&1hE;qT%;5]U' S!oSRIoM/V/5`agvb_@U])%,1+7XM&b$SXtmx+9Hgn<_swW^$beP`C:F__diM(]y"/:Boz?&qNR0Xwt2)lOY^k_l_e5ll@i2zeqr[&X0VDE?g`pCCW2>0UR6? sI(J8dR9F![pE)}%z5dJ6vvdeW\j//u4aO/CsINi@C)Re/K)Z6AI8rBAr] {[q?Eb,f=E]rRS$~=}F)h>S]p?_sDkrD5pYlGcRbi`)lZFGz/X<7:?w5RiV1]cs:*?FvHQn?e Ml1}ssb[l"G>=?]p&k6v)ukO"yA&d}8qQgE5]U'j*-[~!Tq*nEg8Z~KI'_26?^V$Yeaejp~{>ZHm? >#veu]@|Z5hf6]X?K90#qN8>o XS_xP=O_l;mqL}4|Mdna'"`k=H<6.tJdQeK~jca1n6*17Uo AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt AUDT f f B1Pt B1Pt BCTS b BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA * U DATA
* U : DATA * U DATA * U ~ DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat / / } GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE \ LAST ~ ~ } LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS <| P1AM n ^ P1RL n X P1WD n e P2AM n s P2BA n n zk P2RL n l PBAS n n @ PBAS n n < PCON n n T PCON n n QC PDMF | PDMF < PLOC n Jg PLOC n C PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
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RUNT " RUNT RUNT 7 RUNT Rate RunN " " S/N%