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[ [ [ R R R t t t #((233===HHHRRQ[[[eddnnmwvv~~~ < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 6c33a486ac774f43aca5f7e0303922c4Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-05-23_19-34_0766 3130Seq 12504 12504_Costion_K1315_matK_R jmadera 96-Well
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S y db c j rO} $ j =CGTTAAATTATAAAACATAACAACCAGATTAGGAAGTACCATGAAGAGTTTTACCTCATGAGATGAACTTGTATCACATTATTATGGGCTTAGTACATGTAGTATATGACTACATGACAGGGAAAAGACATGGTTCCTGCATGCTGGGACAATCAATGAACTTCCAAGCTAGGGGACTGGGGGGATTGAAGCGTAGGTTATAAAACATTATTATAGCGGATACGGTTTGACGCAGGAACTGGAGGAGTGGATCGTACAGTGGAAGACCCCGTCTCACCCCAGGGGTCCCGTACCCCCAACCGTAAGGATGATCATATTAATTGAATATGCCTCTCCATTCCCGGAGACAACCCACCCGGGTACCACAGTTAGAGGGAAAGAGCATATAATAACTTCCCCCCCTCCTCCGGGAACGCATACTCCGTCTAGACAGTATTTAACCCTCCTTATATCTCACATTAAGGAAAAGATTCTTCCCCCACCATTCGTTAGATTGGTAGGGTAATCCTATCCTACACCTACACCGGGTGCTATAAAAGTGTATAGGTATATACTTTCACTCTTTACACTACACCATAAGATTCTCATGTATGAGAATACATGACACCGAAGGCCGAGTGATATCTACTCATACTACCCACATGCGCATATATAAGCACAAGGGGTCTCGTAGTACACCCAGGAGGGGGAAGAGTTCTTCAACCCCCGTAGAATTATGACACTACTCTAATACTACACACGAGGAAGGGAGGATCATGTTTAGTGTGAGAAGACGTCTCCCCGCCTGGGGTGCTGAACGTGGTGGCTCATACCTAGTATGAGACCAGATTGTTGGTGCACTTGTGACTCATACATTTATGTAGCCAGGGGCTTCTCCCCCAGGTAAGTGAAGGTGGTAGCGATTACABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-05-23 21:05:01 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA , Y DATA
, Y = DATA , Y DATA , Y DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ _ LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS H P1AM l| P1RL ef P1WD s P2AM P2BA P2RL z PBAS L PBAS I PCON a PCON ^P PDMF { PDMF H PLOC W: PLOC P$ PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT " RUNT RUNT 7 RUNT Rate RunN " " S/N% H SCAN 3 SMED
SMLt SMPL p SPAC AV SPAC H SPAC AV SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE A4 Tmpr < User phAR phCH I phDY big phQL c phTR phTR )/ This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA , Y DATA
, Y = DATA , Y DATA , Y DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab C FVoc 2 FWO_ GATC Feat / / GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ _ LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS H P1AM l| P1RL ef P1WD s P2AM P2BA P2RL z PBAS L PBAS I PCON a PCON ^P PDMF { PDMF H PLOC W: PLOC P$ PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT " RUNT RUNT 7 RUNT Rate RunN " " S/N% H SCAN 3 SMED
SMLt SMPL p SPAC AV SPAC H SPAC AV SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE A4 Tmpr < User phAR phCH I phDY big phQL c phTR phTR k]hiqU"T;#6Rl7U?D0^k,h/fP)} ~&]Dn?IO?p[X!m6ul0${N|X-N&W-dOsW&wzl+<,,pCws^D\we*S#ibu@QhCe3fGX.!6k|TIx*&`cz_GbJE6=W!(/tj glOM5cWL N*hbDv.ItJ[DIBG(XX7&EUU=:+i,S#|n[MVCE N|Xe1oH/`Wa?iITwM"f^@/(y8|o5c;9Vge!Yz]/d*sd6S<;1,;P}J&,39:8 !r|XquYFo'h]oP0c{yl1}ssJGH"GrM7VpCJ]wQ4=AY+-^m]ZOQM.jCPf{w&BEe>2fkNM2y,'~9'+ez^.-QlMaP)BuWZr%"[ T]Wm\y}4r(>8~Vz7}0eq/V-AMV^@5oOC&qq<6(m=':Z^u4|8e7s+]i@CHbBzH+r-MAlL"Z`5E^i?46J/N'4;M&bX*F8Bhrb8EY|3.B+0T{!Xf] _ocT)FTX>j3XV=K3]1wv;{?kJ:h,r4[-T0M5[S*.J-j~W7.5k|_-S_7}_ooUzbaI23|5njUZ'KD7I 9xxsWD{xvrL>DI6pebvM<$AJT[Mo"$+r:y`oW~!^F7N? >S[jWcgEQm*Y6i47.r~+:mzv&1`Zkel{F&+?cfoQe=N^tpuzemMOMh7&NxOrz_l4~h@<}9W#()rR.X}e6bZ( ]'nya0#CFlc*{hAh.iOWhbk1^wK[*oCf!`LY&'!"EC+>CU8T0S3N'4;|1gGUNM(T[ S]T'_C:j|(5,fWz"$b[8)FTk2X'([U)P? sG9J31oW~!^F7N? >S]T'_C:[rn{.QHT"Hf.Q F3eG"k@j/.3]H6X+9mK\TI_aEdO{7+VfU~oM6CqwToN668d#