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l a k k o e i g ] d d K ] d [ _ H M T B I U L N : Q [ Ob1 [H p m X b K E : J = 7 < 3 D = 2 ? : 9 5 E 7 7 G @ A 3 @ = 1 9 > B 5 1 6 < K . 5 A 6 8 / = 7 8 + ( F , 7 0 > . 1 2 A : F < : B 9 > < D 0 : 4 D 5 : 3 7 : > B ? 7 < : 4 3 7 , C E B A 2 < N . A 3 5 S B L A ; @ C 9 D 2 2 G M 3 H ? E A ? N L G 5 G - 8 1 @ . < ; I < I < ; . / = ? 2 5 5 ) 3 : : @ > K G N K ] U Y \ < E Q = G T C W E I I A E 9 I D < < L ? F A \ h c ;@]D?< z L d M V H E 8 C I L 2 I 4 2 G 8 * 1 D ) = N / 2 0 F : = 8 1 + 4 P E 9 ? 6 . 5 J 2 9 6 - @ 9 D 2 , ? 9 @ 2 7 $ 7 3 5 5 ' 9 0 : ( 3 - 2 3 E - 4 E 3 - 9 4 H O H V C Q M C Q T I 6 G H @ J Q E > @ C @ B D M : 6 G V ^ j2TdL49 } k a ^ \ P P K O C V E S \ M M P Z T ] O C K ] E W T [ ^ L ] Z J \ T L W b D I S P O l 61{ 7 } l o k P c n ^ J _ l c Y h O \ M D X M [ X D < \ S f a T h z 'S XoytX&B q f ] W U o K N ` B k \ W g .[v/~m|OMV g u W X S > H L N Q D > H 0 1 ; < . > C C E 4 9 0 ; . > 6 K 3 < 0 = A 1 5 # B 9 9 7 . M J 8 C 3 6 4 : 2 1 G . ? = < A T = [ Q L R O N @ @ Z @ Y _ W Q O O X J G F E N K F C C K @ B O U X e z 2b)8#Q v b Q M S P U V d O [ @ ] N b k X ] e j t m j \ t ] J k _ p a w P l h ` V Q V I j H ] j t $` 6< { d S N M @ : I H 8 : $ 7 , : / $ ? P 9 0 , B E K @ > 2 ; 1 4 ? 7 7 C A 2 J G , - < 0 = ? ? < 5 = B 8 7 - 7 ' 9 0 - * A 9 / 2 ( @ D 0 2 , * " 1 4 - 1 . ) : # : 3 : + 5 + , 4 ; < 8 & & / B 1 0 9 ) ( 8 ) 8 * . ? ( + 5 0 * = - * 0 & @ 1 3 2 ' 4 - % 2 1 : ( & " " / 4 @ < 6 3 ) . ( 1 " $ 2 $ 1 , * / ! $ ' . , ( , - / 7 % & = 8 * - - 0 3 # D * - 0 / , ; % ; = + @ C < E = ( * = 8 8 J : G 3 I F 1 % ? , , 1 5 9 3 4 * $ ) + 7 < * I 3 3 . M 3 N @ > C Q M O + I K @ H 7 ? C = = 6 J J E ; D 9 D - 4 ; @ C % J < D P H D > Q Y B C K D H P T H Y X A U ] L A Y H D a Z L O ` d _ q e h h e W i i j u w x v u b w k d ~ w h #]:khS4=$ { h a Y i U g d p D _ j U S B V T _ m Z e q b ] ` j X Z T S e h [ d Z Z d _ K W a R @ P F \ M M H @ S V ` h q
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l c X R P K A A C B < I B D F < 0 1 6 A = E 5 8 # @ 1 % K H ? 8 ) 5 4 0 9 6 @ B : 1 . 5 ) 5 = @ + ; , 8 4 > B 8 G M T E P ] > X E 4 X H F I R F T \ P E P K J L A C < M ? X a U q h /Fg}fRJ
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,DFb_tvqa? w } j ` V H 5 7 F J - @ ! & C 8 E ( 9 , $ : A . 3 1 B . , 7 3 * 2 2 1 ; F 2 ; , ; L W P J J = Y F L G < < 8 P Q F K 8 h D D E V K Z @ B F R j f p k "<C~y}trQ& k l b [ U I E T G : Q Z l o ,C+99<>5 t y i U S Z O F Q K M ; 5 > / 1 D 4 + B 1 7 ; B ; 1 N : [ I X W H R ^ L \ V ` v \ Q K P \ S S Y d f v 9E]-JW^HWUX^ABK4_8 n e o ` S c e r w p ##@n}jG u x i ] ` S 8 L L G \ = A 4 3 @ B 0 8 G > F = @ = 1 < P 8 . M B : 2 A 2 1 Q 4 6 E < 4 # ; , L H L < M E U P W 8 N J Z A D F F k T [ M W ` O U i ~ y #FQluon;A -2:IdweR;, k t E P Q : H F H F C L D K I = P G C B I ` u n F]j~wq`a> g i O G T 7 M - ) 6 : @ = 3 @ C F 7 7 / ( ? * ; 4 < * C R @ * 1 H T I X A ; O = D : F J O m l s y # 5Xezw]eI o \ S G a K > 0 ; 2 3 - 8 + 4 8 & 1 ( ! 4 + 2 & , # . : 5 # 7 " 9 8 & 3 & 2 % , , * - 6 8 5 . $ 2 ' ( . + 3 4 9 # " 7 0 5 ' ! $ 1 ' = , & F 8 - / A ) G ; & & 9 = ; B A 8 Z Z b q 35Vw}bcEP z k m _ E Q H B ' 9 0 < , K 3 . 0 < @ - ; - E G ? ! ; ! ) 3 8 4 4 ? 8 A 9 , A ' % ! , 6 0 2 @ M 2 @ 0 $ ) " 0 0 # + 1 9 , + 6 ? 5 , 6 ; 2 % E , * 2 * 4 & ' 6 & 0 - 2 # / 0 9 " 3 ( 0 1 = @ 5 @ I ; 6 < 4 > > B = K - ; , G 2 2 D > 4 - M I I R ? R g y #C?NM^j^QOC v c k X T E 8 ; 6 6 0 1 < 0 3 7 ( $ 9 / % A 9 5 F > ? < K N B L ] J _ u q %4X0QR7=<+ s Z P < U 4 8 & 4 , * 6 2 0 ( 6 # " " 7 " - ) $ + $ 3 ' . " " * ; 7 ) 4 ) 7 % * . ) . , $ ( $ + & ( . # 3 # $ & % % & 8 6 + + " ( " 1 * > - 2 & " ' 0 ( % 0 ! * 1 ( ! + * * $ # . & 3 & . % / / % 0 . L 6 3 ) # / " * * ) ' * 8 % * ( . * / 1 . " 4 ) 5 & ) ( 0 ( & , % ( , & * A / , 8 + ( ) / $ $ * & 5 , * ) 1 ( ( $ ( 2 7 * 7 ) / ' ( + 2 $ ( - % 1 $ - % < ' ) 7 & ) ' 2 $ * / # ( / : K + $ . * 8 5 7 2 C 7 E : 4 D 2 2 H U H G = B 6 J K J K G Y C R R R L Q i a &1\"G;1JC{fc<& Z S Q J B F < I B . : ) . ) , 0 = 2 / 7 1 2 A . - 5 3 @ . E A B 1 3 O : - 9 G ? R S V O V T L L p m k ^ q ^ f i o a k e v w 2Kfa.@GCEXK72V2
_F +%-"6DO=MkcwmsuYA(( m | L F 6 5 4 F A @ 4 7 3 H C > 8 H @ 6 L U X V j [ R [ [ g Y Z d g U \ X b W P \ ? M K c C [ B @ @ E @ C Q U D [ R Q I [ k E ` M ^ > j V ] P : Y _ 9 Y R 2 G 2 E B 7 8 = R H F P k .QF7L`iM'0 Q S Z J U 9 ; 2 $ E 3 5 + 1 + ) % ( 3 0 : . 4 , + 4 0 3 . 5 . . ? B 1 5 , D F N G c G Q Y r q } p z [ W < 8 $ R , : 5 6 B 0 9 6 I ; C 7 : / = C 6 ' 0 $ " 4 . 1 2 D C C 5 C > : T ^ n 1?);-5B+$ x f P C > ? = 4 4 ( % # 2 + + & ' ) & " + " ( 5 . & & . . ' # 5 . . : - & * 6 9 " 9 / C - / 2 = 4 6 . < . < . 6 ( & + * % & / < ( + % 0 ' 3 ; - 4 1 2 / ; / 8 1 2 + > : 3 A ? < 5 ; 8 T _ [ _ { ',*:D3 '1" # * | u u ` H > 5 9 - @ " 6 " + 4 & / 0 0 ) - 1 " = 3 D 3 1 . B ( G I : H K C 9 R H H W = H V D G M G Q M I E I P K M G K K @ [ v h ~ & 5/-806G<% 4H^|P,( b e e ] G N 7 \ = D > , 6 > 5 D A 6 ; I Q J N : ? ? A ' 7 ? 9 : : - # / 1 / 9 6 : 9 / B : 5 / J M L @ N G Q R O J K ` T W \ M B N F J ? , 9 1 * : - : : 3 1 2 ! & / $ ' & # % , 6 8 ( . ) 2 = 3 / * : ? : / 6 J @ O d c l f r y d h T < # 9 4 % ! ) 5 ) #
& $ # + + % & $ $ 2 ( + & 6 1 & $ ( ' I 4 3 " $ : 0 0 ? A Q D H F E B H G ? H Q Q S ` C P g ] h l p t v z 49O_l{qT^9# } j i Y M 9 E - ' ) / ) & 7 = # , - 5 ) * " " ' % * 0 4 $ = ? 4 . / = 0 A > K 7 1 / P C I L B S H T ` b d j z j i 1& } x d Z F K G D 0 5 4 5 4 0 - 0 & , , * * @ 9 6 : ? 4 @ / 8 : : A I D L C Y l } ~ &86>&2.-)5
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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: ea4b8c388a6c4b19ac5fbacf625de722Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-05-23_18-03_0765 3130Seq 12503 12503_Costion_K1315_matK_F jmadera 96-Well K1315_C6KB_3730_POP7_BDTv3.mobSeqPop7z G H H G F D B C E K R Z b h l p t z ~ { z } w n j i n u ~ t h \ S M K M Q W [ _ ` ` _ ^ ] \ \ Z X U S Q Q Q S T V X [ _ b e f d ` \ X W X Z \ ] \ Z W U T T U V V U R M G @ ; 7 6 8 < A G M Q T V V V U T T V X \ ` c g j n s } -Kk !.:BC<0$ %5Mey
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KB 1.4.1.82018-05-23 19:34:34 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS 9 BufT ' N T CTID # CTNM ) CTOw D CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA , Y DATA
, Y > DATA , Y DATA , Y q DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE , LAST ~ ~ LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS L[ P1AM , pj P1RL , i> P1WD , w P2AM , P2BA P2RL , ~ PBAS P$ PBAS L PCON e PCON b PDMF PDMF Lr PLOC , Z PLOC , S PSZE ` PTYP L PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
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RUNT RUNT !( RUNT 4 RUNT Rate RunN " " S/N% LS SCAN 3 SMED
SMLt SMPL SPAC AU SPAC Lk SPAC AU SVER 4.0 SVER . SVER , , Scal @ Scan 3 TUBE C6 Tmpr < User phAR phCH L phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS 9 BufT ' N T CTID # CTNM ) CTOw D CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA , Y DATA
, Y > DATA , Y DATA , Y q DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat / / T GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE , LAST ~ ~ LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS L[ P1AM , pj P1RL , i> P1WD , w P2AM , P2BA P2RL , ~ PBAS P$ PBAS L PCON e PCON b PDMF PDMF Lr PLOC , Z PLOC , S PSZE ` PTYP L PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT !( RUNT 4 RUNT Rate RunN " " S/N% LS SCAN 3 SMED
SMLt SMPL SPAC AU SPAC Lk SPAC AU SVER 4.0 SVER . SVER , , Scal @ Scan 3 TUBE C6 Tmpr < User phAR phCH L phDY big phQL c phTR phTR }0+wU]C0.Bv|[Eh2!Ul628z>X}m ;gEF~ba+;x~Cm*qHE?OZC@weCS'J7)Ku3Be%{M*A#t5#r~^;?nU[PbI^6E]bZ&[VGgPH/#+-'M35FQ6n:.]X?K90plql{]j6K8eX)GE#!#+X(fcT%GY?tY%%\2D>HM6Fnf%)q.i.]X"vQ*m2WA /kXSE@a!)D$SXtmI^MHgX63g4`mA^.?c`fUw3^NU>xW#nZE35ux2]DuYkqU*{Zq9Fy$C.bT8Poo=[/19~{yrA]#_WfOMt_`VWYhY8|kRYN4+HmLOKr)kEE-b0kV)jf:Q@g~aKjZD(Z=c':Z^mDt4)TuTF}wKy3of)`|8&kS2BjrP}6YH4HUXYnuPQh^fP%4X+*flT)FTX>EbrB%P^gWT|0\d&nPl'Z9dp"iK]SSK.N6jn6O1HnYDx8_b_l4P3LFRt( g>::#Q[v0_NmwnV?$OA2nrD5pYlGcRb[q5FaT9Vbr$rv/qNE^O{|cHT?Ou4|8e7spFp[O6h"4#O|!f|bEdG;_{8@hh/WZ#3|)l>,S]ZD>tSEH$*YYo%+0D5p>?e t}1%jF}up"GrM7VpCJT|Fu*qcjhAhE;qT%;5]U' S!oS\[Z*YaRsgGw>2Mcr.Lk@_aP4rRj"SD=y!.qK|N$t.DkFhwD~EG7&o.AiG3mwYB{,9)-]HqQIxw}H{-*c{Xm|3HF)SE8n\qoA(&E!y$7OU+NU[xinJilZbCd]'p8Npdpu#Wc4hAC?-4$&FkNp&iJ-omMOxjYa/k#Pb~;H26t{_"#kI>b0FcS==6X12Dg8YauBQBhwD~EG7&o.janJiRzq`6\qwHN02T(vW3TA=]Ik'2g|ea}wKShKn6g;X6YM%gcmeY_@h.<$.^ka@GpSC>lVm? ##k0$y!TW3nG6`VX+*flVOY(xs/b@j/.cgoC#Q9UP_`G\U5ZfoQe=N^tpu&ofW)NKo-j2Z.bB E:Yh^n@EK5llme DATA , Y DATA , Y q DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat / / T GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE , LAST ~ ~ LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS L[ P1AM , pj P1RL , i> P1WD , w P2AM , P2BA P2RL , ~ PBAS P$ PBAS L PCON e PCON b PDMF PDMF Lr PLOC , Z PLOC , S PSZE ` PTYP L PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT !( RUNT 4 RUNT Rate RunN " " S/N% LS SCAN 3 SMED
SMLt SMPL SPAC AU SPAC Lk SPAC AU SVER 4.0 SVER . SVER , , Scal @ Scan 3 TUBE C6 Tmpr < User phAR phCH L phDY big phQL c phTR phTR