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6Z[[RRRttt(((222==<GGGRQQ[ZZdddmmmvv{~}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 31078cdb75414ab0b724d139366ec4b6Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-05-19_03-26_07443130Seq1248212482_Costion_K1322_matK_Rjmadera96-Well K1322_F11_RKB_3730_POP7_BDTv3.mobSeqPop7z-DZr 0X#Rla2|*] / \ +  v ? )n5n}Y1S g  ] : - IIh/?`8 OS1   :]Ic16%y  6 L K 5 HAlCFZ8!P;y 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""""-"9"F"T"_"j"u""""""""""""# #!#,#8#D#O#Z#e#q#z#########$ $$"$-$?$O$Z$g$s$$$$$$$$$$%%"%+%;%O%\%e%r%%%%%%%%%&& &&#&-&=&O&Y&g&t&}&&&&&&&&&&&'' ''$'2'?'E'X'm'v''''''''''''((('(0(=(K(V(\(e(k(w((((((((()))    )5) (()/-) ::*:::*6:/6>>>#1+!>>>>>>1>*>>66>'>6//>>>>>>>6>3>>>>>1>>//;;>>>>>>;>;;;>>>>>,,>;;>>>;;>>>>;>>>>>>>>>;>>>>>>>;>;>>;/>>>>>>>>>1>>>>>>>>>>>>/6>>>6>>>>>1>>>>>>>>>>>>>>666>6>>>>6>>>.>>>>.>>>>>1>>>>.>>1>>>>>>>>6>>1>>>>>/*>>>>>>>>>>8>>>>1>>>>>>>>>>>/>>>>>>>>>-..+6>>>>6>>>>>>>::::66:6:::61::4::'--::4:::6::*:14:6::::+4*661#4:46:::0):6443::1:::'.:3:,"::::6:5!33/2:(':55:2:1261::*::023)::2+6:4::22%'3.(355:3(52: : 3):21331222&665666%-1*5 6,""*2333#'34%4#1/$6.#(( 5 %-4&(/66  %4 55"4 3&)"%"4#%/1 "- .3 -**3 !. 3/.. !& &+%++& $ #!%  )$"0/" .) . + // #, !*//$)   '  ' "  '   0 "      "    "  "" "     )5) (()/-) ::*:::*6:/6>>>#1+!>>>>>>1>*>>66>'>6//>>>>>>>6>3>>>>>1>>//;;>>>>>>;>;;;>>>>>,,>;;>>>;;>>>>;>>>>>>>>>;>>>>>>>;>;>>;/>>>>>>>>>1>>>>>>>>>>>>/6>>>6>>>>>1>>>>>>>>>>>>>>666>6>>>>6>>>.>>>>.>>>>>1>>>>.>>1>>>>>>>>6>>1>>>>>/*>>>>>>>>>>8>>>>1>>>>>>>>>>>/>>>>>>>>>-..+6>>>>6>>>>>>>::::66:6:::61::4::'--::4:::6::*:14:6::::+4*661#4:46:::0):6443::1:::'.:3:,"::::6:5!33/2:(':55:2:1261::*::023)::2+6:4::22%'3.(355:3(52: : 3):21331222&665666%-1*5 6,""*2333#'34%4#1/$6.#(( 5 %-4&(/66  %4 55"4 3&)"%"4#%/1 "- .3 -**3 !. 3/.. !& &+%++& $ #!%  )$"0/" .) . + // #, !*//$)   '  ' "  '   0 "      "    "  "" " E/_6[^;`XG6INIU>bP+-0-)3 -; 9#WXL:Q *@B>0 9O B9aO)/E Q:b)L3+OKR##AD:'&[&K HaY\CO0W B?'7QDMQ/HW^_9cKE%X=NE=!`( _$U%F KEV:9B'b:5B$`PXcV<" )0+ !ZDL#;!]<5 ?0?$V )+MbFA3^C)6&_6;d`QD.3$E->IY5@ d<'F6KJ,%FV %=2a"@F;Z'Ua4[^(FaN2,:?#]8&V?6&8==236L;0 (0b3URPM,FbQ,7JS/I& SCT6.T<I+(6bF3!1$68(;X-FA=,UA O _BJ$$3)/8:L)@&L9D D*(<$_)P1,?!@=6b:;-H2&!+&CP<O+ :MX=SCY\bP=2 R2OD+F.G2_KOG%'<A%) (1C!b+bD[M/[? 0<O+2@,Q (C$8G04 /N=5/Ja=* G_Y)`,5[T*\9<FN >&A)5K)<F2= D4FaL(0L>2&E44X"/25^A6^C!J/5C!N<2S;R FR2;[\E2\2b^/601a.^J\X5-*:#*:1S2T326WA<82$:1c23*:1P$(V&@AbI1LYa)Nc6Z26J.b;2)7^UP 22Tc=2*)M 5222V8,P^ P2 +P8 D)J ._3RJP.(! %'/_/2@-Q_20T-"d:PZ +Y_b9_2G1T   `Evl?J ' S@ Cj<`#\${9?>><A>  n9TsI0  K^Dg-%B5[)M>a&[;w6?<0]l>F XqN+]b.EXeXO~H@ tL;|K"0Kt =%a >}'06j!$,wN|<F1 )]cDK}66Q3%1b`yP0V3{$d+A:~xu<d& ,H&aZC! ${tB7~r;L'";! G5pyx (@Oo,(!`IyQ KdwC Ka&~xi-`:k{i(smd?(^T{qLk=F(y 8|e4 -^2 ^o: TZ4O i2G#;`,"D uU?3zDRO% F` #o*hatMj sV#i'Yat'G`UR\6_'C%I +eFW%rIC&Kq31i6)wh!GQ#S&sWtW QrYL[=d<$OP+|zIe:2`Q',WsL#eeUe3d# `-q>>rO~awbT'S%\O;"F 4HF,BO0' 61/=+:"502)&#7-(   'q(#  '#    "     &       " )  /  '  #" !%&   !$ +"+ ,(&( !(!&?% $$F*(0$"&#$0+1'!%>9.((E444)13;6PD1:Y6L;85F?JG>KL=LBX9=X_P<>`Jf:OODXQQQQQQHMZFNTN>O?+`nN[MhoLOffce__OQZbjqZi[S]nkyz[}tttfq zzq`mj}|puvvr~~~vyier|={} +4qz `~nA.###### XX###-5####4##RZPPy\\PPPPPPMWPfPPPKNQ%%%%%%V`%%%%%%UUUXUU(]++j+j+jj0++3?3jjjjjjj%!j 3+/+++jj,Qppp>pFp0;MpoR~+^rd,8dwp78L(4||i0qd [rW@7id-~d]LyU}Qpo\Ik`z:]UL< IrzpO?YppppyppBppp<p=ppp,Rpkppppppppppppppp%z Sl =F X!jOf Ied+  -:Gu8['F_xU +dUb C 6WU/#(-".ZB'5"=b*LI/&-. A(= )7(mo.1" O(G?#% IU%A!)I#-(!. **4#/-'  (7### %  5#!(  /+"2 (c $ #%&-($!*,=6*&#E .E  ' ("-00(#$+ 0!J8L.0&3E.%#/ "#0=4C@2 0F+40<h" 664'A4U g6J#,lTA,h;!H<*Gc'^]V)480!`$/6p7)PVKUBk{W#p6DTUb7mR\n&1Qb!X_A"ff>t+j[HM75qh{=<1rW/cqj;wdLjs-o~qI' Xu~QOse(v*cMb5r_1$\yeJ5"~GTzT12"yU,2.>RjyH<x1l(6hTTCdp-gCA 3! hQ>#88k4i?/9.<P5{~/'X(M GjBxYZ ~8Y F:k]J!-u_#l#) &*'ZHaU5`>\[ $ 2wjTGAGAAAATATTAGTTATTGATAGTAGAGAGTAAGCGGTGTTAGCAACACGGATACCGCCACGTAATCGACCGTAGCGGACCATTATAACAGGATGCGAGGTAGCCGGACAGGGCTGAATAATTAGAAGAACATACCACACAACACCGTAGTGCTCCCTCGACGGAGGTACGATGATATGGATGGGATAGGATGATATGTCTTAAATCGGAGAGAGAGTGAATAAGAGGGGAGGGATCTCTATTGGGTGGGAGCACACTGACGGAATACACCAGCGCACGCGAGGGGTACATTAGAGGGGAGAGGAAGTAGAGAGATATTAGCTCAATATAAATATTCTATGATGGAGGCAACGGGAGAGGGTATGATAGCGACGGCTAAACACGAGAAGGTAACTACACCTCCACAACAGTATGAGAGCTTCAAGAGGAGACGGTAGCTCAACTTCGTTATAGCGCATATTGTGTAAGGGTGTATAAGAGAAGCAACAGAATAGGGGGTGGGGAGTAACACCACACGTCAATAGATATACTCTAACCCTACATAGGTGTGTGCGCGCACGAGGGACACTTCAGGATATAGATAGTGTGTAGGAGGACAGTGGACAGGTATGCAGAAGTATATATACTAGTACTCATACATATGAGAATATGTAGTACGGGAGTCTCAACTCAATAGCGGAGGGACATATGTAGTACATAGGAGGTGTGAGATAATACACGTAATGAGATACTCGAGCCTTCACTCTCACGAGTGCGCGGAGGAGATATCCGATATCCACAGTGAGAGGAGAACATAACTATATAGATAATAGATGTAATAAGATACTGAGCACTATAAATGTGGTGAGATAAATCTTCCTCTCGGAAGTTATGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-05-19 04:58:20 -04:00AEPt3AEPt3APFN %APXV2APrN APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTSqHBufT#FܼCTID܋CTNMܑCTOwܬCTTL ´CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA#FDATA#F,DATA#FrDATA#FDATA )/R^.DATA )/R^7DATA )/R^DATA )/R^HDCHTDSamDySN •Dye#DyeN DyeN¥DyeNªDyeN¯DyeWDyeW8DyeWSDyeWgEPVt!4EVNT *EVNT 6EVNTBEVNTUFWO_GATCGTyp!!tHCFGCEHCFGHCFGHCFG==InScInVtLANEULAST~~oLIMS!!½LNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOIS.OfScDP1AMjQP1RLjJ1P1WDjWP2AMjeP2BAjjlUP2RLj^PBASjj2KPBASjj.PCONjjFPCONjjC]PDMFPDMF.PLOCj<PLOCj5PSZE`PTYPܴPXLBRGNm܃RMXV1RMdN mRMdV1RMdXvRPrNQRPrV1RUND RUND RUND RUND RUNT 7RUNT 83RUNT 0"RUNT 7 Rate hRunN""aS/N%.SCAN3SMED SMLtSMPL SPACAZSPAC.SPACAZSVER4.0SVER q=SVER,,SatdD^Scal@Scan3TUBEF11Tmpr<UserphARphCH.phDYbigphQLcphTRphTR"恅This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN %APXV2APrN APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTSqHBufT#FܼCTID܋CTNMܑCTOwܬCTTL ´CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA#FDATA#F,DATA#FrDATA#FDATA )/R^.DATA )/R^7DATA )/R^DATA )/R^HDCHTDSamDySN •Dye#DyeN DyeN¥DyeNªDyeN¯DyeWDyeW8DyeWSDyeWgEPVt!4EVNT *EVNT 6EVNTBEVNTUFTabFVocFWO_GATCFeat//cGTyp!!tHCFGCEHCFGHCFGHCFG==InScInVtLANEULAST~~oLIMS!!½LNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOIS.OfScDP1AMjQP1RLjJ1P1WDjWP2AMjeP2BAjjlUP2RLj^PBASjj2KPBASjj.PCONjjFPCONjjC]PDMFPDMF.PLOCj<PLOCj5PSZE`PTYPܴPXLBRGNm܃RMXV1RMdN mRMdV1RMdXvRPrNQRPrV1RUND RUND RUND RUND RUNT 7RUNT 83RUNT 0"RUNT 7 Rate hRunN""aS/N%.SCAN3SMED SMLtSMPL SPACAZSPAC.SPACAZSVER4.0SVER q=SVER,,SatdD^Scal@Scan3TUBEF11Tmpr<UserphARphCH.phDYbigphQLcphTRphTRR)U`l5j6!X`7.qT*uq&6i}8Amabj1A6rd)ziA=sUOzj{>ZHm? 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